Click here to switch to the map view.

The map label for this gene is citF [H]

Identifier: 147674991

GI number: 147674991

Start: 346114

End: 347718

Strand: Direct

Name: citF [H]

Synonym: VC0395_A0326

Alternate gene names: 147674991

Gene position: 346114-347718 (Clockwise)

Preceding gene: 147673483

Following gene: 147674374

Centisome position: 11.45

GC content: 52.21

Gene sequence:

>1605_bases
ATGGGCCCATCATCGAACGCGCACGTTGGACACTGCAACGTGCTGAATCCGGCACCAAACACTAAAGGTCATCTCATGAG
TATCAATACATTAAATAACCCTCTGTCTGTGCTGGAAGTGGAACGCTATGCGTTGACGCCTTACACCCAAGCGCACGCGA
TCACGCCGCATCTGCTGGATGAGCCTGCCAAGAAAAACCGTAAGGTGAAAAGTTCGATCCGCGAAGCGATTGTCGATCTT
GGCTTAAAAGATGGCATGACCATCTCTTTCCACCATGCATTTCGTGGCGGCGATAAAACCGTCAATCTGGTGATGGAGGA
GATTGCTGAATTGGGGATTAAAAACCTCACGCTCGCCTCAAGCTCGCTCACCTCTTGTCATTCGCCTTTGATTGAACACA
TCAAAAACGGCGTGGTGAGCAAGATTTACACCTCAGGGATCCGCGGAGCGCTGGCCGACAGCATTTCTAACGGCCTGCTT
GATGAGCCAGTGCATATTCATTCGCACGGTGGTCGTGTACATCTAATCCAAAGTGGTGAGCTGTACATCGACATGGCGTT
TATTGGCGTGCCTTGTTGCGATGAGTTTGGTAACGCCAACGGTTTTAAAGGTAAATCCAATTGTGGCTCACTCGGCTATG
CCAAAGTTGATGCCGAGCATGCTGAAAAAGTGGTGATGCTGACCGAAGCGATCGTCGGTTTCCCGAATTTCCCTGCATCG
ATTACTCAAGATCGTGTTGATGCGGTGGTACAAGTAGCCGAAGTGGGCGACCCAAGCAAAATCGGTGGGGATGCCACGCG
CATGACCACCAACCCACGCGAGCTGTTGATTGCCAAACGCGCTGCGGAAGTCATTGAGCATTCTGGCTATTTCTATGATG
GTTTTTCAATGCAGACTGGTTCGGGCGGTGCATCGCTCGCGGTTGCGCGCTTTTTGAAAGAGAAGATGCTGCGCCAAGAC
ATCCGTGCTTCTTTCGCTCTCGGTGGTATCACCGCAACCATGGTCGATATGCATGAGCAGGGGCTGATTGATTACTTGCT
CGATGTGCAGTGTTTCGATTCGGTGGCGGCGGGCTCGTTAGCGCGCAATCCGCATCATCTCGAAATCTCTGCTAACGAAT
ACGCCAACCCAAGCTCGAAAGGGGCGGCGGTGGATAGGCTGGATGTGGTTATCTTGAGTGCGCTGGAGATTGATACCCAG
TTTAACGTTAACGTGATCACCGGCTCTGATGGGGTAATTCGCGGTGCATCCGGCGGCCACTGCGACACGGCGGCTGCGGC
AAACTTATCGATTATTGTGGCGCCTTTGGTGCGTGGCCGCATTCCGACCTTGGTCGAGAAGGTCACTAACGTCATCACCC
CAGGATCCACCATTGATGTGTTGGTGACGGATCAAGGTATCGCGGTAAACCCGAATCGTCCCGAACTGAAAGCACGTTTC
ATCGAGGCGCAACTGCCCGTTGTCGAGATTGAAGCGTTGCAACAGCGTGCCGAGTTGCTGACGGGCAAACCCCAGCCGTT
GCAATTTGAAGACAAAACCGTTGCCTTTGTTCACTACCGTGACGGTTCGATCATTGATGTTATCAAACAGGTAAAAAGCC
TATGA

Upstream 100 bases:

>100_bases
ACCCAAAAAGAGGTCGATCACGCCATCGCAGTCATCGAAGCCGCCGAAGAAGCCGCGGCGAAAGGGCTGGGCGTGGTTTC
CCTTAACGGCAAAATGGTCG

Downstream 100 bases:

>100_bases
GTCACCATCCGGATCTTTCAGTCAGTCTGGACCAGCTCCTTCTGCGTAAAGAAGTGCGGGTTCGGCAACAAGGTGAGTGG
CTAAAGCGCCATTCACTGCC

Product: citrate lyase, alpha subunit

Products: NA

Alternate protein names: Citrase alpha chain; Citrate (pro-3S)-lyase alpha chain; Citrate CoA-transferase subunit [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MGPSSNAHVGHCNVLNPAPNTKGHLMSINTLNNPLSVLEVERYALTPYTQAHAITPHLLDEPAKKNRKVKSSIREAIVDL
GLKDGMTISFHHAFRGGDKTVNLVMEEIAELGIKNLTLASSSLTSCHSPLIEHIKNGVVSKIYTSGIRGALADSISNGLL
DEPVHIHSHGGRVHLIQSGELYIDMAFIGVPCCDEFGNANGFKGKSNCGSLGYAKVDAEHAEKVVMLTEAIVGFPNFPAS
ITQDRVDAVVQVAEVGDPSKIGGDATRMTTNPRELLIAKRAAEVIEHSGYFYDGFSMQTGSGGASLAVARFLKEKMLRQD
IRASFALGGITATMVDMHEQGLIDYLLDVQCFDSVAAGSLARNPHHLEISANEYANPSSKGAAVDRLDVVILSALEIDTQ
FNVNVITGSDGVIRGASGGHCDTAAAANLSIIVAPLVRGRIPTLVEKVTNVITPGSTIDVLVTDQGIAVNPNRPELKARF
IEAQLPVVEIEALQQRAELLTGKPQPLQFEDKTVAFVHYRDGSIIDVIKQVKSL

Sequences:

>Translated_534_residues
MGPSSNAHVGHCNVLNPAPNTKGHLMSINTLNNPLSVLEVERYALTPYTQAHAITPHLLDEPAKKNRKVKSSIREAIVDL
GLKDGMTISFHHAFRGGDKTVNLVMEEIAELGIKNLTLASSSLTSCHSPLIEHIKNGVVSKIYTSGIRGALADSISNGLL
DEPVHIHSHGGRVHLIQSGELYIDMAFIGVPCCDEFGNANGFKGKSNCGSLGYAKVDAEHAEKVVMLTEAIVGFPNFPAS
ITQDRVDAVVQVAEVGDPSKIGGDATRMTTNPRELLIAKRAAEVIEHSGYFYDGFSMQTGSGGASLAVARFLKEKMLRQD
IRASFALGGITATMVDMHEQGLIDYLLDVQCFDSVAAGSLARNPHHLEISANEYANPSSKGAAVDRLDVVILSALEIDTQ
FNVNVITGSDGVIRGASGGHCDTAAAANLSIIVAPLVRGRIPTLVEKVTNVITPGSTIDVLVTDQGIAVNPNRPELKARF
IEAQLPVVEIEALQQRAELLTGKPQPLQFEDKTVAFVHYRDGSIIDVIKQVKSL
>Mature_533_residues
GPSSNAHVGHCNVLNPAPNTKGHLMSINTLNNPLSVLEVERYALTPYTQAHAITPHLLDEPAKKNRKVKSSIREAIVDLG
LKDGMTISFHHAFRGGDKTVNLVMEEIAELGIKNLTLASSSLTSCHSPLIEHIKNGVVSKIYTSGIRGALADSISNGLLD
EPVHIHSHGGRVHLIQSGELYIDMAFIGVPCCDEFGNANGFKGKSNCGSLGYAKVDAEHAEKVVMLTEAIVGFPNFPASI
TQDRVDAVVQVAEVGDPSKIGGDATRMTTNPRELLIAKRAAEVIEHSGYFYDGFSMQTGSGGASLAVARFLKEKMLRQDI
RASFALGGITATMVDMHEQGLIDYLLDVQCFDSVAAGSLARNPHHLEISANEYANPSSKGAAVDRLDVVILSALEIDTQF
NVNVITGSDGVIRGASGGHCDTAAAANLSIIVAPLVRGRIPTLVEKVTNVITPGSTIDVLVTDQGIAVNPNRPELKARFI
EAQLPVVEIEALQQRAELLTGKPQPLQFEDKTVAFVHYRDGSIIDVIKQVKSL

Specific function: Represents a citrate:acetyl-ACP transferase [H]

COG id: COG3051

COG function: function code C; Citrate lyase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786832, Length=468, Percent_Identity=71.3675213675214, Blast_Score=695, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006472 [H]

Pfam domain/function: PF04223 CitF [H]

EC number: =4.1.3.6; =2.8.3.10 [H]

Molecular weight: Translated: 57149; Mature: 57018

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPSSNAHVGHCNVLNPAPNTKGHLMSINTLNNPLSVLEVERYALTPYTQAHAITPHLLD
CCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCHHHEEEHHHCCCCCCHHHCCCHHHHC
EPAKKNRKVKSSIREAIVDLGLKDGMTISFHHAFRGGDKTVNLVMEEIAELGIKNLTLAS
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECC
SSLTSCHSPLIEHIKNGVVSKIYTSGIRGALADSISNGLLDEPVHIHSHGGRVHLIQSGE
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCC
LYIDMAFIGVPCCDEFGNANGFKGKSNCGSLGYAKVDAEHAEKVVMLTEAIVGFPNFPAS
EEEEEEEECCCCHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCH
ITQDRVDAVVQVAEVGDPSKIGGDATRMTTNPRELLIAKRAAEVIEHSGYFYDGFSMQTG
HHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCEEECEEEECC
SGGASLAVARFLKEKMLRQDIRASFALGGITATMVDMHEQGLIDYLLDVQCFDSVAAGSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARNPHHLEISANEYANPSSKGAAVDRLDVVILSALEIDTQFNVNVITGSDGVIRGASGGH
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHEEECCEEEEEEEECCCCEEECCCCCC
CDTAAAANLSIIVAPLVRGRIPTLVEKVTNVITPGSTIDVLVTDQGIAVNPNRPELKARF
CCCHHHCCCEEEEEHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCHHHHHH
IEAQLPVVEIEALQQRAELLTGKPQPLQFEDKTVAFVHYRDGSIIDVIKQVKSL
HHHCCCCHHHHHHHHHHHHHCCCCCCCEECCCEEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
GPSSNAHVGHCNVLNPAPNTKGHLMSINTLNNPLSVLEVERYALTPYTQAHAITPHLLD
CCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCHHHEEEHHHCCCCCCHHHCCCHHHHC
EPAKKNRKVKSSIREAIVDLGLKDGMTISFHHAFRGGDKTVNLVMEEIAELGIKNLTLAS
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECC
SSLTSCHSPLIEHIKNGVVSKIYTSGIRGALADSISNGLLDEPVHIHSHGGRVHLIQSGE
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCC
LYIDMAFIGVPCCDEFGNANGFKGKSNCGSLGYAKVDAEHAEKVVMLTEAIVGFPNFPAS
EEEEEEEECCCCHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCH
ITQDRVDAVVQVAEVGDPSKIGGDATRMTTNPRELLIAKRAAEVIEHSGYFYDGFSMQTG
HHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCEEECEEEECC
SGGASLAVARFLKEKMLRQDIRASFALGGITATMVDMHEQGLIDYLLDVQCFDSVAAGSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARNPHHLEISANEYANPSSKGAAVDRLDVVILSALEIDTQFNVNVITGSDGVIRGASGGH
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHEEECCEEEEEEEECCCCEEECCCCCC
CDTAAAANLSIIVAPLVRGRIPTLVEKVTNVITPGSTIDVLVTDQGIAVNPNRPELKARF
CCCHHHCCCEEEEEHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCHHHHHH
IEAQLPVVEIEALQQRAELLTGKPQPLQFEDKTVAFVHYRDGSIIDVIKQVKSL
HHHCCCCHHHHHHHHHHHHHCCCCCCCEECCCEEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]