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The map label for this gene is vibD [H]

Identifier: 147674931

GI number: 147674931

Start: 327151

End: 327936

Strand: Direct

Name: vibD [H]

Synonym: VC0395_A0309

Alternate gene names: 147674931

Gene position: 327151-327936 (Clockwise)

Preceding gene: 147675717

Following gene: 147674202

Centisome position: 10.82

GC content: 49.75

Gene sequence:

>786_bases
TTGTGTCGTTATGCCCGATCCTATCTCCTGCACGCCTTTGATTATACCTTATTCGTTAAAGCGCGGTTAAACATGGCTTT
TATGATTTCAATGCCAGCGATTGAGCTGGCAGGGCTGACAATTCAGGTTTGCCAATTCGATCCTCAGCAATTTGATGAGG
ATCAGCAGCACTATTTAGCCCCTCCCACCTACCATAGCCTGCACAAGGCCGTGGTTAAGCGCCAAGCGGAATTTGTCGCA
GGACGTAAGTTAGCGCAGCAAGCGCTCAAACAGATCGGGCAGGGTTATGATAGGCCAATTGCTATCGGAACCCATCGTGA
GCCACTATGGCCTGCGGGGATCACGGGCTCGATCGCCCATTGTGACGGCTGGGCAGTATGCACTGTGCTTAAGGCAGAAC
ACCTCTCGCTTGGTATTGATATTGAACATCGGCTCGCACATCAAACCGCTAGTGAGGTGCAAGCTATAATCGGCACCGCC
CAAGAGTGGGCACTGTTGGCGCAACAGTTCGATCTCGCTTCAGCGGTGACTTTGCTGTTTTCTGCAAAAGAGAGTCTATT
TAAAGCACTATTCCCACAAGTACACCTCTACCTTGATTTCTGCGATGCCCAGCTCAGTTCACTGGAGCCAAACAAAATGA
GTTTCACATTGACGCGCGTACCTAATGCCGCACAACAGAGTTGTGAGGTTTACTTTCGTTGGTTTGAACAACAGCGAGTA
TTGACCGTCAGCGTGTTACCAATGCTAGGCAAAATCGAGTATCATGTCCCCACCTTACCTACTTGA

Upstream 100 bases:

>100_bases
CTGACCATCTGATTGTGTTGCAAGAAGGCAAAATCGTCGCGCAGGGCGATCCACACTCTTTGGTGACTCCAGCATTGGTT
AAGCAAGTCTTTGGGATTGA

Downstream 100 bases:

>100_bases
GTTAACTTATTGATTATGCTGCGTGATTATGATCTAAACCTGCTGACGGTATTTGATTCTGTCATGAAATTAGGCTCAGT
CAGTAAAGCAGCGGATAAGC

Product: vibriobactin synthase component D

Products: pyrophosphate; AMP; enterobactin; aryl carrier protein/isochorismatase; 3',5'-ADP; serine activating enzyme + [C]

Alternate protein names: Enterobactin synthase component D; Enterochelin synthase D [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MCRYARSYLLHAFDYTLFVKARLNMAFMISMPAIELAGLTIQVCQFDPQQFDEDQQHYLAPPTYHSLHKAVVKRQAEFVA
GRKLAQQALKQIGQGYDRPIAIGTHREPLWPAGITGSIAHCDGWAVCTVLKAEHLSLGIDIEHRLAHQTASEVQAIIGTA
QEWALLAQQFDLASAVTLLFSAKESLFKALFPQVHLYLDFCDAQLSSLEPNKMSFTLTRVPNAAQQSCEVYFRWFEQQRV
LTVSVLPMLGKIEYHVPTLPT

Sequences:

>Translated_261_residues
MCRYARSYLLHAFDYTLFVKARLNMAFMISMPAIELAGLTIQVCQFDPQQFDEDQQHYLAPPTYHSLHKAVVKRQAEFVA
GRKLAQQALKQIGQGYDRPIAIGTHREPLWPAGITGSIAHCDGWAVCTVLKAEHLSLGIDIEHRLAHQTASEVQAIIGTA
QEWALLAQQFDLASAVTLLFSAKESLFKALFPQVHLYLDFCDAQLSSLEPNKMSFTLTRVPNAAQQSCEVYFRWFEQQRV
LTVSVLPMLGKIEYHVPTLPT
>Mature_261_residues
MCRYARSYLLHAFDYTLFVKARLNMAFMISMPAIELAGLTIQVCQFDPQQFDEDQQHYLAPPTYHSLHKAVVKRQAEFVA
GRKLAQQALKQIGQGYDRPIAIGTHREPLWPAGITGSIAHCDGWAVCTVLKAEHLSLGIDIEHRLAHQTASEVQAIIGTA
QEWALLAQQFDLASAVTLLFSAKESLFKALFPQVHLYLDFCDAQLSSLEPNKMSFTLTRVPNAAQQSCEVYFRWFEQQRV
LTVSVLPMLGKIEYHVPTLPT

Specific function: Catalyzes the transfer of the 4'-phosphopantetheine moiety from coenzyme A to apo-domains of both entB (an ArCP domain) and entF (a PCP domain). Plays an essential role in the assembly of the enterobactin [H]

COG id: COG2977

COG function: function code Q; Phosphopantetheinyl transferase component of siderophore synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. EntD family [H]

Homologues:

Organism=Escherichia coli, GI1786797, Length=188, Percent_Identity=38.8297872340425, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278
- InterPro:   IPR003542 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 29419; Mature: 29419

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRYARSYLLHAFDYTLFVKARLNMAFMISMPAIELAGLTIQVCQFDPQQFDEDQQHYLA
CCHHHHHHHHHHHHHHEEEEECCCEEEEEECCCHHHCCCEEEEEECCHHHCCCCCHHCCC
PPTYHSLHKAVVKRQAEFVAGRKLAQQALKQIGQGYDRPIAIGTHREPLWPAGITGSIAH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEC
CDGWAVCTVLKAEHLSLGIDIEHRLAHQTASEVQAIIGTAQEWALLAQQFDLASAVTLLF
CCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
SAKESLFKALFPQVHLYLDFCDAQLSSLEPNKMSFTLTRVPNAAQQSCEVYFRWFEQQRV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCE
LTVSVLPMLGKIEYHVPTLPT
EEEEEHHHHCCEEECCCCCCC
>Mature Secondary Structure
MCRYARSYLLHAFDYTLFVKARLNMAFMISMPAIELAGLTIQVCQFDPQQFDEDQQHYLA
CCHHHHHHHHHHHHHHEEEEECCCEEEEEECCCHHHCCCEEEEEECCHHHCCCCCHHCCC
PPTYHSLHKAVVKRQAEFVAGRKLAQQALKQIGQGYDRPIAIGTHREPLWPAGITGSIAH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEC
CDGWAVCTVLKAEHLSLGIDIEHRLAHQTASEVQAIIGTAQEWALLAQQFDLASAVTLLF
CCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
SAKESLFKALFPQVHLYLDFCDAQLSSLEPNKMSFTLTRVPNAAQQSCEVYFRWFEQQRV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCE
LTVSVLPMLGKIEYHVPTLPT
EEEEEHHHHCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate; Coenzyme A; apo-EntB; apo-serine activating enzyme [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin Coenzyme A + apo-EntB = aryl carrier protein/isochorismatase + 3',5'-ADP Coenzyme A + apo-serine activating enzyme = 3',5'-ADP + serine activating enzyme [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]