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The map label for this gene is leuO [H]

Identifier: 147674202

GI number: 147674202

Start: 327946

End: 328872

Strand: Direct

Name: leuO [H]

Synonym: VC0395_A0310

Alternate gene names: 147674202

Gene position: 327946-328872 (Clockwise)

Preceding gene: 147674931

Following gene: 147673392

Centisome position: 10.84

GC content: 47.03

Gene sequence:

>927_bases
TTGATTATGCTGCGTGATTATGATCTAAACCTGCTGACGGTATTTGATTCTGTCATGAAATTAGGCTCAGTCAGTAAAGC
AGCGGATAAGCTAGGTATGACTTCCGCAGCGGTGAGTCAAAATTTATCACGCTTACGAGAACAAGTTGGCGATCCGTTGT
TTATCCGTCAGGGACGTGGGTTACAGCCGACGCAGTATGCGCTCAATATGCACAAACATGTGGCGGAAGGCTTGAGTGCC
ATTCGTTTTGGTTTAGAAACCGATGCCGGTTTTGATCCTGCAACCAGTAAGCGTGAGTTTATTATTGGCGGTCAGGGCTA
TTTCGATTTGGTGGTATTACCGCCACTTTTGCAAAAAATCAGCGACATCGCCCCGCATATCACGGTGAATTTAAAGTCTT
ATGAGGACGATCATTTCACACCAAGCCAAGTATTAAGTGAGCGAGAGGCGGATCTGTTTTTGGCTGCATTACCCATCAGC
CATCCCTCTATCGTCACCACACAGGTGGCGGAGGAGGCCTTGGTTGTGGTGTATGCCAAAAATCATCCGCGTCTTGGAAG
TACCCTCAGTTTTGAGCAGTTTTTTGCAGAAAAACACACTGCATTAACCAGTCGTCGCTTTGGTAATTATCTCTTTTCTA
GCCTAGTCGATCAGGTGCTGCCCGCACGTCGAGTGCATTATCAAAGTGAGTCGATGCTCAATTTGATGGCGACCGCATCC
GTCACCGATCTGCTCTGTTTTACTCCAAAGCGTTTAGCGGATATGTGGGCGGAGAAATTGGGTTTACAGATCCAACCTTT
GCCGTTTGAAATCCGCTCGGTTCCGACTTTTATGTGCTGGCATAAAGCCAAACAGCAAGATAAAGGGCTGATTTGGCTGC
GCGAGCAAATTGAGGCGATTCTCTGCGCCAATCCTCAATCTATTTAA

Upstream 100 bases:

>100_bases
TCGTTGGTTTGAACAACAGCGAGTATTGACCGTCAGCGTGTTACCAATGCTAGGCAAAATCGAGTATCATGTCCCCACCT
TACCTACTTGAGTTAACTTA

Downstream 100 bases:

>100_bases
GGTCACACAATTTATTGGCTGCGTTCTTGTTTCTCTCCATGCCATAGCGTGTCTTCGCGTATGGTGATTCACTCAAGTGC
TCTCTCGCGGCAGCGCCAAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MIMLRDYDLNLLTVFDSVMKLGSVSKAADKLGMTSAAVSQNLSRLREQVGDPLFIRQGRGLQPTQYALNMHKHVAEGLSA
IRFGLETDAGFDPATSKREFIIGGQGYFDLVVLPPLLQKISDIAPHITVNLKSYEDDHFTPSQVLSEREADLFLAALPIS
HPSIVTTQVAEEALVVVYAKNHPRLGSTLSFEQFFAEKHTALTSRRFGNYLFSSLVDQVLPARRVHYQSESMLNLMATAS
VTDLLCFTPKRLADMWAEKLGLQIQPLPFEIRSVPTFMCWHKAKQQDKGLIWLREQIEAILCANPQSI

Sequences:

>Translated_308_residues
MIMLRDYDLNLLTVFDSVMKLGSVSKAADKLGMTSAAVSQNLSRLREQVGDPLFIRQGRGLQPTQYALNMHKHVAEGLSA
IRFGLETDAGFDPATSKREFIIGGQGYFDLVVLPPLLQKISDIAPHITVNLKSYEDDHFTPSQVLSEREADLFLAALPIS
HPSIVTTQVAEEALVVVYAKNHPRLGSTLSFEQFFAEKHTALTSRRFGNYLFSSLVDQVLPARRVHYQSESMLNLMATAS
VTDLLCFTPKRLADMWAEKLGLQIQPLPFEIRSVPTFMCWHKAKQQDKGLIWLREQIEAILCANPQSI
>Mature_308_residues
MIMLRDYDLNLLTVFDSVMKLGSVSKAADKLGMTSAAVSQNLSRLREQVGDPLFIRQGRGLQPTQYALNMHKHVAEGLSA
IRFGLETDAGFDPATSKREFIIGGQGYFDLVVLPPLLQKISDIAPHITVNLKSYEDDHFTPSQVLSEREADLFLAALPIS
HPSIVTTQVAEEALVVVYAKNHPRLGSTLSFEQFFAEKHTALTSRRFGNYLFSSLVDQVLPARRVHYQSESMLNLMATAS
VTDLLCFTPKRLADMWAEKLGLQIQPLPFEIRSVPTFMCWHKAKQQDKGLIWLREQIEAILCANPQSI

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=297, Percent_Identity=33.6700336700337, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1786820, Length=284, Percent_Identity=30.2816901408451, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI87081766, Length=265, Percent_Identity=25.2830188679245, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790147, Length=300, Percent_Identity=25.3333333333333, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34503; Mature: 34503

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMLRDYDLNLLTVFDSVMKLGSVSKAADKLGMTSAAVSQNLSRLREQVGDPLFIRQGRG
CEEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCC
LQPTQYALNMHKHVAEGLSAIRFGLETDAGFDPATSKREFIIGGQGYFDLVVLPPLLQKI
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCEEEEEHHHHHHHH
SDIAPHITVNLKSYEDDHFTPSQVLSEREADLFLAALPISHPSIVTTQVAEEALVVVYAK
HHCCCEEEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCEEEEEEEC
NHPRLGSTLSFEQFFAEKHTALTSRRFGNYLFSSLVDQVLPARRVHYQSESMLNLMATAS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
VTDLLCFTPKRLADMWAEKLGLQIQPLPFEIRSVPTFMCWHKAKQQDKGLIWLREQIEAI
HHHHHHCCHHHHHHHHHHHHCCEECCCCCHHHCCCHHHHHHHHHHCCCCEEEHHHHHHHH
LCANPQSI
CCCCCCCC
>Mature Secondary Structure
MIMLRDYDLNLLTVFDSVMKLGSVSKAADKLGMTSAAVSQNLSRLREQVGDPLFIRQGRG
CEEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCC
LQPTQYALNMHKHVAEGLSAIRFGLETDAGFDPATSKREFIIGGQGYFDLVVLPPLLQKI
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCEEEEEHHHHHHHH
SDIAPHITVNLKSYEDDHFTPSQVLSEREADLFLAALPISHPSIVTTQVAEEALVVVYAK
HHCCCEEEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCEEEEEEEC
NHPRLGSTLSFEQFFAEKHTALTSRRFGNYLFSSLVDQVLPARRVHYQSESMLNLMATAS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
VTDLLCFTPKRLADMWAEKLGLQIQPLPFEIRSVPTFMCWHKAKQQDKGLIWLREQIEAI
HHHHHHCCHHHHHHHHHHHHCCEECCCCCHHHCCCHHHHHHHHHHCCCCEEEHHHHHHHH
LCANPQSI
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3413113; 1630901; 9278503; 3519576 [H]