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The map label for this gene is lysS [H]

Identifier: 147674731

GI number: 147674731

Start: 201361

End: 202899

Strand: Direct

Name: lysS [H]

Synonym: VC0395_A0195

Alternate gene names: 147674731

Gene position: 201361-202899 (Clockwise)

Preceding gene: 147673826

Following gene: 147675556

Centisome position: 6.66

GC content: 49.77

Gene sequence:

>1539_bases
ATGCAAATGACTGATGCTGTTCAAAACGAGATTAATCAAGAGCAAATCGCGCAAGAAGAAAACAAACTGATTGCAGAGCG
TCGCAGCAAGTTGGATCACATCCGCAAGAACTGTAAAGCAAACGGGCACCCAAACTCGTTCCGTCGCGACAGCCTAGCGG
GTGATCTGCAGAAGAAGTTCGGTGAAAAGAGTAAGGAAGAACTGGAAGCGCTCAATCACGTGGTGTCTATCGCTGGTCGT
GTGATGGCAAAACGTGGCCCATTCTTGGTGATTCAAGAAACCTCTGGCCGCATTCAGGCTTATGCATCGAAAGAAGTGCA
ACAAGAGCTGAAAGACAAATACCAAGGTTTGGATATCGGTGACATCATCGGTGTGCAAGGTGCCCTGCACAAATCCGGTA
AAGGCGATTTGTACGTCAACATGGAGCAATTCCAACTGCTGACCAAAGCACTGCGCCCGCTGCCAGAGAAATTCCACGGT
CTGACTGACCAAGAAATGCGTTACCGCCAGCGTTACGTTGACCTGATCGTCAACGAAAACTCTCGCAACGCTTTCATTGT
ACGTTCTAAGGTGATGTCAGCCATTCGTAACTTCATGATCTCAAAACAGTTCATGGAAGTGGAAACGCCGATGATGCACG
TGATCCCTGGTGGTGCGAGTGCGCGCCCATTCATCACGCACCACAACGCGCTCGACATGCCGATGTACCTGCGTATCGCG
CCTGAGCTGTACCTGAAGCGTCTGGTTGTCGGTGGTTTTGATCGTGTATTCGAAATCAACCGTAACTTCCGTAACGAAGG
TCTTTCTCCACGTCACAACCCAGAATTCACCATGATGGAATTCTATATGGCGTACGCGGACTACAAAGATCTGATGGATC
TGACGGAAGAGCTGCTCAGCTCTGTGGCGTTAGAAGTTCTCGGTTCAACCTCCATGCCTTACGGTGAGCATACCGTTGAA
TTTGGTGGTAAATACGCGCGTATGAGCATGTTTGATGCGATCAAGCACTACAACCCAAATCACGCGCAGATCCAAGCTCT
GACCGAAGAAGACATCCAAAACCGTGACCTAATGGTTTCTATCGCGAAATCCGTTCACGTTGATGTGGAGCCTTTCTGGA
CCTGTGGTCAGCTTCTCGAAGAGATCTTTGGTGAAACGGCTGAGCCGAAACTGATGCAGCCAACTTTCATCACTGGCTAC
CCAGCAGATATCTCTCCACTGGCACGTCGTAGCGATGACAACCCGTTCTTCACTGACCGCTTTGAGTTCTTCATCGGTGG
CCGTGAAGTGGCAAACGGCTTCTCAGAGCTGAACGATGCTGAAGACCAAGATGCGCGTTTCAAAGCCCAAGTGGAAGCGA
AAGAGTCTGGTGATGATGAAGCCATGTTCTATGATGCCGACTACATTACCGCTTTAGAGCACGGCTTACCGCCAACAGCG
GGTCAAGGTATTGGTATTGATCGTCTGGTTATGCTCCTGACCAATACTCACACTATCCGTGATGTGATTTTGTTCCCAGC
GATGCGCCCTCAGCAATAA

Upstream 100 bases:

>100_bases
AAACCGCAACACGCAAGCGGTACTCGATGGTGACTTAGACAAATTTATCGAAGCTAGCCTGAAATCAGGCCTGTAAGCTT
TTTACCGACAAACAAGGTAC

Downstream 100 bases:

>100_bases
TCACTGTAGATTGATTAAAAAGCCGCTCTATTGAGCGGCTTTTTTTATTTCCTCAAGAAATAATCGTGCCAAGTCGGTGC
AATGTAAGTCATTCTTTTTA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MQMTDAVQNEINQEQIAQEENKLIAERRSKLDHIRKNCKANGHPNSFRRDSLAGDLQKKFGEKSKEELEALNHVVSIAGR
VMAKRGPFLVIQETSGRIQAYASKEVQQELKDKYQGLDIGDIIGVQGALHKSGKGDLYVNMEQFQLLTKALRPLPEKFHG
LTDQEMRYRQRYVDLIVNENSRNAFIVRSKVMSAIRNFMISKQFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIA
PELYLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSVALEVLGSTSMPYGEHTVE
FGGKYARMSMFDAIKHYNPNHAQIQALTEEDIQNRDLMVSIAKSVHVDVEPFWTCGQLLEEIFGETAEPKLMQPTFITGY
PADISPLARRSDDNPFFTDRFEFFIGGREVANGFSELNDAEDQDARFKAQVEAKESGDDEAMFYDADYITALEHGLPPTA
GQGIGIDRLVMLLTNTHTIRDVILFPAMRPQQ

Sequences:

>Translated_512_residues
MQMTDAVQNEINQEQIAQEENKLIAERRSKLDHIRKNCKANGHPNSFRRDSLAGDLQKKFGEKSKEELEALNHVVSIAGR
VMAKRGPFLVIQETSGRIQAYASKEVQQELKDKYQGLDIGDIIGVQGALHKSGKGDLYVNMEQFQLLTKALRPLPEKFHG
LTDQEMRYRQRYVDLIVNENSRNAFIVRSKVMSAIRNFMISKQFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIA
PELYLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSVALEVLGSTSMPYGEHTVE
FGGKYARMSMFDAIKHYNPNHAQIQALTEEDIQNRDLMVSIAKSVHVDVEPFWTCGQLLEEIFGETAEPKLMQPTFITGY
PADISPLARRSDDNPFFTDRFEFFIGGREVANGFSELNDAEDQDARFKAQVEAKESGDDEAMFYDADYITALEHGLPPTA
GQGIGIDRLVMLLTNTHTIRDVILFPAMRPQQ
>Mature_512_residues
MQMTDAVQNEINQEQIAQEENKLIAERRSKLDHIRKNCKANGHPNSFRRDSLAGDLQKKFGEKSKEELEALNHVVSIAGR
VMAKRGPFLVIQETSGRIQAYASKEVQQELKDKYQGLDIGDIIGVQGALHKSGKGDLYVNMEQFQLLTKALRPLPEKFHG
LTDQEMRYRQRYVDLIVNENSRNAFIVRSKVMSAIRNFMISKQFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIA
PELYLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSVALEVLGSTSMPYGEHTVE
FGGKYARMSMFDAIKHYNPNHAQIQALTEEDIQNRDLMVSIAKSVHVDVEPFWTCGQLLEEIFGETAEPKLMQPTFITGY
PADISPLARRSDDNPFFTDRFEFFIGGREVANGFSELNDAEDQDARFKAQVEAKESGDDEAMFYDADYITALEHGLPPTA
GQGIGIDRLVMLLTNTHTIRDVILFPAMRPQQ

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=507, Percent_Identity=42.0118343195266, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI194272210, Length=507, Percent_Identity=42.0118343195266, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI40789249, Length=244, Percent_Identity=25.4098360655738, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1790571, Length=498, Percent_Identity=60.4417670682731, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1789256, Length=496, Percent_Identity=60.2822580645161, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI87082379, Length=335, Percent_Identity=30.7462686567164, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1788173, Length=157, Percent_Identity=30.5732484076433, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=504, Percent_Identity=40.2777777777778, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17535927, Length=504, Percent_Identity=40.2777777777778, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71994340, Length=504, Percent_Identity=40.2777777777778, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32566633, Length=311, Percent_Identity=26.0450160771704, Blast_Score=96, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320242, Length=512, Percent_Identity=41.796875, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324256, Length=390, Percent_Identity=31.025641025641, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6323011, Length=355, Percent_Identity=24.7887323943662, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24640851, Length=510, Percent_Identity=40.9803921568627, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640849, Length=510, Percent_Identity=40.9803921568627, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24584738, Length=244, Percent_Identity=27.8688524590164, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 58337; Mature: 58337

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMTDAVQNEINQEQIAQEENKLIAERRSKLDHIRKNCKANGHPNSFRRDSLAGDLQKKF
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
GEKSKEELEALNHVVSIAGRVMAKRGPFLVIQETSGRIQAYASKEVQQELKDKYQGLDIG
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHH
DIIGVQGALHKSGKGDLYVNMEQFQLLTKALRPLPEKFHGLTDQEMRYRQRYVDLIVNEN
HHHCCCHHHCCCCCCCEEEEHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHEEECC
SRNAFIVRSKVMSAIRNFMISKQFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIA
CCCEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCHHHHCC
PELYLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLS
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SVALEVLGSTSMPYGEHTVEFGGKYARMSMFDAIKHYNPNHAQIQALTEEDIQNRDLMVS
HHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCHHHEEEECHHCCCCCHHHEE
IAKSVHVDVEPFWTCGQLLEEIFGETAEPKLMQPTFITGYPADISPLARRSDDNPFFTDR
EHHHHEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHCCCCCCCCCCH
FEFFIGGREVANGFSELNDAEDQDARFKAQVEAKESGDDEAMFYDADYITALEHGLPPTA
HHHEECCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEECCHHHHHHHHCCCCCC
GQGIGIDRLVMLLTNTHTIRDVILFPAMRPQQ
CCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQMTDAVQNEINQEQIAQEENKLIAERRSKLDHIRKNCKANGHPNSFRRDSLAGDLQKKF
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
GEKSKEELEALNHVVSIAGRVMAKRGPFLVIQETSGRIQAYASKEVQQELKDKYQGLDIG
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHH
DIIGVQGALHKSGKGDLYVNMEQFQLLTKALRPLPEKFHGLTDQEMRYRQRYVDLIVNEN
HHHCCCHHHCCCCCCCEEEEHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHEEECC
SRNAFIVRSKVMSAIRNFMISKQFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIA
CCCEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCHHHHCC
PELYLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLS
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SVALEVLGSTSMPYGEHTVEFGGKYARMSMFDAIKHYNPNHAQIQALTEEDIQNRDLMVS
HHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCHHHEEEECHHCCCCCHHHEE
IAKSVHVDVEPFWTCGQLLEEIFGETAEPKLMQPTFITGYPADISPLARRSDDNPFFTDR
EHHHHEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHCCCCCCCCCCH
FEFFIGGREVANGFSELNDAEDQDARFKAQVEAKESGDDEAMFYDADYITALEHGLPPTA
HHHEECCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEECCHHHHHHHHCCCCCC
GQGIGIDRLVMLLTNTHTIRDVILFPAMRPQQ
CCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]