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The map label for this gene is phoB [H]

Identifier: 147674650

GI number: 147674650

Start: 262977

End: 263666

Strand: Direct

Name: phoB [H]

Synonym: VC0395_A0253

Alternate gene names: 147674650

Gene position: 262977-263666 (Clockwise)

Preceding gene: 147675451

Following gene: 147675436

Centisome position: 8.7

GC content: 49.86

Gene sequence:

>690_bases
ATGTCTAGAAGGATTCTGGTTGTTGAAGACGAAGCACCGATTCGTGAAATGTTGTGTTTCGTGCTTGAACAAAAGGGCTA
TCAGGCGGTTGAGGCTGAAGATTATGATTCGGCGATGAGTAAGCTGGCTGAGCCTTTTCCTGATCTCGTCCTGCTTGACT
GGATGTTGCCTGGTGGTAGTGGCATCAACCTCATCAAGCATATGAAGCGCGAAGAGATGACGCGCAACATTCCTGTTGTG
ATGCTCACCGCCCGTGGTGAAGAAGAAGATAAAGTTCGCGGCTTAGAAGTGGGCGCGGATGATTACATTACCAAACCGTT
TTCACCTAAAGAGTTGGTTGCCCGTTTAAAAGCGGTGATCCGCCGTGTCACTCCGACGGCGTTGGAGGATGTGATTGATG
TTCAAGGTTTGAAGTTAGATCCGGTCTCACACCGTGTGACCGCTAACGATCAGCCGTTGGATATGGGACCGACTGAGTTC
AAGATGTTACACTTCTTCATGACACACCAAGAGCGTGTGTATAGCCGCGAGCAGCTACTCAATAATGTTTGGGGCACCAA
CGTGTATGTGGAAGATCGCACGGTAGACGTCCACATTCGTCGTCTACGTAAAGCCTTAGAAGACGCTGGACATGATAAGC
TGATCCAGACGGTGCGCGGCGCGGGGTATCGTTTTTCAACCAAAGCCTAA

Upstream 100 bases:

>100_bases
GCCTGAACCTTATATGAAAATTTGTTTGCAAAGGTCACAAAAGTGTCATAAACATTTCCCATAATGCATGGCGTTGGTGA
AAAACAAAGGTTAATCAATT

Downstream 100 bases:

>100_bases
GAGGGTGTAGAACAAATCAGTATGGTTGAACGTTTAACATGGAAAAAGCTGGCTTGGGAGCTGGCTTTTTTTTACTTGCC
ATGGGTTGTGGTGGGATGGA

Product: DNA-binding response regulator PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDSAMSKLAEPFPDLVLLDWMLPGGSGINLIKHMKREEMTRNIPVV
MLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVIRRVTPTALEDVIDVQGLKLDPVSHRVTANDQPLDMGPTEF
KMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA

Sequences:

>Translated_229_residues
MSRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDSAMSKLAEPFPDLVLLDWMLPGGSGINLIKHMKREEMTRNIPVV
MLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVIRRVTPTALEDVIDVQGLKLDPVSHRVTANDQPLDMGPTEF
KMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA
>Mature_228_residues
SRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDSAMSKLAEPFPDLVLLDWMLPGGSGINLIKHMKREEMTRNIPVVM
LTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVIRRVTPTALEDVIDVQGLKLDPVSHRVTANDQPLDMGPTEFK
MLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=80.7017543859649, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=39.0350877192982, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1789809, Length=232, Percent_Identity=37.5, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=35.1351351351351, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=35, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1790860, Length=224, Percent_Identity=37.5, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=34.9775784753363, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=37.9912663755458, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=33.3333333333333, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=36.3636363636364, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=33.7777777777778, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=32.7272727272727, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=32.6086956521739, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI145693140, Length=238, Percent_Identity=29.8319327731092, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=39.4495412844037, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=29.7520661157025, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790299, Length=116, Percent_Identity=31.8965517241379, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26193; Mature: 26061

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDSAMSKLAEPFPDLVLLDWMLPGGS
CCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCC
GINLIKHMKREEMTRNIPVVMLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVI
CHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRVTPTALEDVIDVQGLKLDPVSHRVTANDQPLDMGPTEFKMLHFFMTHQERVYSREQLL
HHCCHHHHHHHHHHCCCEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA
HHHCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCC
>Mature Secondary Structure 
SRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDSAMSKLAEPFPDLVLLDWMLPGGS
CCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCC
GINLIKHMKREEMTRNIPVVMLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVI
CHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRVTPTALEDVIDVQGLKLDPVSHRVTANDQPLDMGPTEFKMLHFFMTHQERVYSREQLL
HHCCHHHHHHHHHHCCCEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA
HHHCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]