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The map label for this gene is frp [H]

Identifier: 147675451

GI number: 147675451

Start: 259261

End: 259983

Strand: Direct

Name: frp [H]

Synonym: VC0395_A0249

Alternate gene names: 147675451

Gene position: 259261-259983 (Clockwise)

Preceding gene: 147673086

Following gene: 147674650

Centisome position: 8.57

GC content: 45.5

Gene sequence:

>723_bases
ATGAATCCTGTTATTGATACCATACTTAGCCATCGTTCTATTCGTGCTTTTACCCAAGAGCCTATTTCTGCAACGCAACT
TGAGACGATCATTGCTTCTGGTATTGCAGCTTCGTCATCCAGCTTACTGCAAGTGATCTCGGTGATCCGAGTGACCGATG
GCAGTATGCGTGAGCAATTAGCAGAGTTTGCTGGCAATCAAGCTTATGTGGCGAGTGCGGCTGAGTTCTTAGTATTTTGC
ATTGATTATCAGCGGCACTATGCATTGAATCCGAACGTAAAACCTGAATTCATGGAGCTCACTTTAATCGGTGCTGTTGA
TGCGGGGATTATGGCGCAAAACTGTTTGTTAGCGGCAGAGTCGATGGGGTTAGGTGGTGTCTATATTGGTGGTTTACGTA
ATTCACCGAATGAAGTCGATGCTTTGCTTAACTTGCCGCCTTATACCGCGGTATTGTTTGGTATGTGTTTAGGGCACCCA
GCTCAGGAGCCAGAGTTAAAACCGCGCTTGGCTCCGAAGGTGATCTTGCATGAAAACCACTACCAGCCTTTGAATAAAGA
GGATGTCGCAGAATATGACCGAACCATGGTTGAGTATTACCAAAAACGTTCCGGTAATCCTAAACAGCAGGGATGGTCAG
AACAAATTACCGCTAAGCTTTCTGAAGAGTCTCGGCCATTTATGCTCAGCTATTTGCAGCGTAAAGGATTAGCTCTGAAG
TAA

Upstream 100 bases:

>100_bases
ACGTCAGTTGCTCGGTGCTGATTCTCATCTTGCTGTAGTGTTTATACCTTTGCAACGCACCGCTTATTATCTTGATGGAC
AACATTTCTGAGGAGGCCGC

Downstream 100 bases:

>100_bases
TAAAGAATCCCAGCACTTGGCTGGGATTTTCGATTTACGCGAGCCCTTGAATTACCACGAACTTTTCGAGTAGTTGCTCT
TCGGTTTCGCGATGGGCAGG

Product: nitroreductase A

Products: NA

Alternate protein names: Flavin reductase P; NADPH-FMN oxidoreductase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MNPVIDTILSHRSIRAFTQEPISATQLETIIASGIAASSSSLLQVISVIRVTDGSMREQLAEFAGNQAYVASAAEFLVFC
IDYQRHYALNPNVKPEFMELTLIGAVDAGIMAQNCLLAAESMGLGGVYIGGLRNSPNEVDALLNLPPYTAVLFGMCLGHP
AQEPELKPRLAPKVILHENHYQPLNKEDVAEYDRTMVEYYQKRSGNPKQQGWSEQITAKLSEESRPFMLSYLQRKGLALK

Sequences:

>Translated_240_residues
MNPVIDTILSHRSIRAFTQEPISATQLETIIASGIAASSSSLLQVISVIRVTDGSMREQLAEFAGNQAYVASAAEFLVFC
IDYQRHYALNPNVKPEFMELTLIGAVDAGIMAQNCLLAAESMGLGGVYIGGLRNSPNEVDALLNLPPYTAVLFGMCLGHP
AQEPELKPRLAPKVILHENHYQPLNKEDVAEYDRTMVEYYQKRSGNPKQQGWSEQITAKLSEESRPFMLSYLQRKGLALK
>Mature_240_residues
MNPVIDTILSHRSIRAFTQEPISATQLETIIASGIAASSSSLLQVISVIRVTDGSMREQLAEFAGNQAYVASAAEFLVFC
IDYQRHYALNPNVKPEFMELTLIGAVDAGIMAQNCLLAAESMGLGGVYIGGLRNSPNEVDALLNLPPYTAVLFGMCLGHP
AQEPELKPRLAPKVILHENHYQPLNKEDVAEYDRTMVEYYQKRSGNPKQQGWSEQITAKLSEESRPFMLSYLQRKGLALK

Specific function: Involved in bioluminescence. It is a good supplier of reduced flavin mononucleotide (FMNH2) to the bioluminescence reaction [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=240, Percent_Identity=51.6666666666667, Blast_Score=248, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26501; Mature: 26501

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVIDTILSHRSIRAFTQEPISATQLETIIASGIAASSSSLLQVISVIRVTDGSMREQL
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
AEFAGNQAYVASAAEFLVFCIDYQRHYALNPNVKPEFMELTLIGAVDAGIMAQNCLLAAE
HHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
SMGLGGVYIGGLRNSPNEVDALLNLPPYTAVLFGMCLGHPAQEPELKPRLAPKVILHENH
HCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCC
YQPLNKEDVAEYDRTMVEYYQKRSGNPKQQGWSEQITAKLSEESRPFMLSYLQRKGLALK
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNPVIDTILSHRSIRAFTQEPISATQLETIIASGIAASSSSLLQVISVIRVTDGSMREQL
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
AEFAGNQAYVASAAEFLVFCIDYQRHYALNPNVKPEFMELTLIGAVDAGIMAQNCLLAAE
HHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
SMGLGGVYIGGLRNSPNEVDALLNLPPYTAVLFGMCLGHPAQEPELKPRLAPKVILHENH
HCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCC
YQPLNKEDVAEYDRTMVEYYQKRSGNPKQQGWSEQITAKLSEESRPFMLSYLQRKGLALK
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8206832; 8885832 [H]