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The map label for this gene is vibA

Identifier: 147674539

GI number: 147674539

Start: 320889

End: 321677

Strand: Direct

Name: vibA

Synonym: VC0395_A0303

Alternate gene names: 147674539

Gene position: 320889-321677 (Clockwise)

Preceding gene: 147674293

Following gene: 147673071

Centisome position: 10.61

GC content: 50.44

Gene sequence:

>789_bases
ATGAAAGATATGTCGTTGGTTAACAGCAAGGTTCTTCTAGTGGGGTCAGCGCGTGGGATAGGGTTCTCCGTGCTTGAACA
TTTATTGCAAGCGGGAGCGCAAGTGATGGCGGCAGATTGTGAGTGGCAGTTGTTGCTAGAGCAGAGTGAGTCGTTATTGG
GGCGTTATCCGGACCAGCTCACTTTAAAAAAACTCGACTTAGCAGAGCCTGAAGCGGTGCGCGAGCAAGTCAATCAATGG
GCTGAGCAAGTGGCGGGATTTGATCATCTGGTCTGCTGTGCAGGAATCCTACATGTTGCGCCATTGCATGAAATGCCAAT
GGAACAAGTGAGTTCTATTTTTACCGTGAATGCTTTTGGTGTGTTGGCCTGTATGCAAGGTGTCGCTTCAAGTATGAAAG
CGCGTCAGCAGGGAAGTATGGTGATTATTGGGTCTAATGCTGCCAATACGCCGCGCATGTCGATTGGGGCTTATGGCGCT
TCGAAAGCCGCATTACACATGCTGGTTAAGTGCATTGGTATGGAGCTAGCCCCTTACGGAATTCGTTGTAATTTGGTCAG
TCCCGGCTCCACTCGCACCGCTATGCAGCAACAGCTTTGGACTGAGCAGTATGGTGAGGCACAAGTCATTGCCGGAGATG
CTGCGCAATTTCGGTTGGGGATCCCGCTCAATAAAATTGCCGAGCCAGCGGATATTGCTCAAGCGGTCCTGTTTTTGTTA
TCCGATAACGCAGGTCACATCACGCTGCATGATTTACGAGTGGATGGTGGAGCCACGCTCGATCATTGA

Upstream 100 bases:

>100_bases
TTCAAATGAGAATAGTTTTTATTAATAAATGCAAGCAATTTCCATTTGGTCTGATAATCAAAGTGATGATTGTGAAGGTT
GATCTCGTTTGTGATAGGGG

Downstream 100 bases:

>100_bases
TCGTTCATCTTTCAACTGGCGATAGCCCGTGTGGAGGTGGTGGCTGCCAATGCCGCAGGCTCTGCGAGTAGGCGTGCAAC
ATCTTGCTCCACCTGTTGGA

Product: 2,3-dihydroxybenzoate-2,3-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MKDMSLVNSKVLLVGSARGIGFSVLEHLLQAGAQVMAADCEWQLLLEQSESLLGRYPDQLTLKKLDLAEPEAVREQVNQW
AEQVAGFDHLVCCAGILHVAPLHEMPMEQVSSIFTVNAFGVLACMQGVASSMKARQQGSMVIIGSNAANTPRMSIGAYGA
SKAALHMLVKCIGMELAPYGIRCNLVSPGSTRTAMQQQLWTEQYGEAQVIAGDAAQFRLGIPLNKIAEPADIAQAVLFLL
SDNAGHITLHDLRVDGGATLDH

Sequences:

>Translated_262_residues
MKDMSLVNSKVLLVGSARGIGFSVLEHLLQAGAQVMAADCEWQLLLEQSESLLGRYPDQLTLKKLDLAEPEAVREQVNQW
AEQVAGFDHLVCCAGILHVAPLHEMPMEQVSSIFTVNAFGVLACMQGVASSMKARQQGSMVIIGSNAANTPRMSIGAYGA
SKAALHMLVKCIGMELAPYGIRCNLVSPGSTRTAMQQQLWTEQYGEAQVIAGDAAQFRLGIPLNKIAEPADIAQAVLFLL
SDNAGHITLHDLRVDGGATLDH
>Mature_262_residues
MKDMSLVNSKVLLVGSARGIGFSVLEHLLQAGAQVMAADCEWQLLLEQSESLLGRYPDQLTLKKLDLAEPEAVREQVNQW
AEQVAGFDHLVCCAGILHVAPLHEMPMEQVSSIFTVNAFGVLACMQGVASSMKARQQGSMVIIGSNAANTPRMSIGAYGA
SKAALHMLVKCIGMELAPYGIRCNLVSPGSTRTAMQQQLWTEQYGEAQVIAGDAAQFRLGIPLNKIAEPADIAQAVLFLL
SDNAGHITLHDLRVDGGATLDH

Specific function: Involved in an early step of the biosynthesis of the catechol siderophore vibriobactin. Vibriobactin is a chelating compound involved in transporting iron from the bacterial environment into the cell cytoplasm

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family

Homologues:

Organism=Homo sapiens, GI7705925, Length=256, Percent_Identity=30.859375, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI32483357, Length=253, Percent_Identity=28.0632411067194, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI5031737, Length=260, Percent_Identity=27.6923076923077, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI19923817, Length=255, Percent_Identity=27.0588235294118, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI15277342, Length=256, Percent_Identity=28.515625, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI40254992, Length=250, Percent_Identity=27.2, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI126723750, Length=263, Percent_Identity=25.4752851711027, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI33667109, Length=195, Percent_Identity=28.7179487179487, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI10190704, Length=257, Percent_Identity=23.3463035019455, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI119392083, Length=195, Percent_Identity=27.6923076923077, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI66933014, Length=233, Percent_Identity=24.4635193133047, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI4503817, Length=196, Percent_Identity=25, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1786812, Length=257, Percent_Identity=39.2996108949416, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1790717, Length=261, Percent_Identity=27.9693486590038, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787335, Length=246, Percent_Identity=31.7073170731707, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=26.2548262548263, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788459, Length=180, Percent_Identity=32.2222222222222, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787820, Length=180, Percent_Identity=26.1111111111111, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI2367175, Length=255, Percent_Identity=27.0588235294118, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786701, Length=221, Percent_Identity=28.0542986425339, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=259, Percent_Identity=32.4324324324324, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71994604, Length=209, Percent_Identity=25.3588516746411, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994600, Length=269, Percent_Identity=24.1635687732342, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560676, Length=266, Percent_Identity=25.5639097744361, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17555706, Length=259, Percent_Identity=28.957528957529, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17508651, Length=254, Percent_Identity=25.9842519685039, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25147288, Length=259, Percent_Identity=26.6409266409266, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193210355, Length=192, Percent_Identity=31.25, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=271, Percent_Identity=27.3062730627306, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=265, Percent_Identity=26.0377358490566, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=257, Percent_Identity=24.9027237354086, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562908, Length=275, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=255, Percent_Identity=27.0588235294118, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=266, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193204405, Length=268, Percent_Identity=26.1194029850746, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=232, Percent_Identity=24.1379310344828, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322226, Length=212, Percent_Identity=26.8867924528302, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24643142, Length=250, Percent_Identity=29.2, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI23397609, Length=266, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571526, Length=258, Percent_Identity=27.906976744186, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21355319, Length=249, Percent_Identity=26.5060240963855, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21357041, Length=171, Percent_Identity=29.8245614035088, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356301, Length=240, Percent_Identity=25, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24644337, Length=193, Percent_Identity=26.9430051813472, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24639444, Length=253, Percent_Identity=24.901185770751, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28571387, Length=244, Percent_Identity=27.8688524590164, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24644339, Length=257, Percent_Identity=24.9027237354086, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): VIBA_VIBCH (Q56632)

Other databases:

- EMBL:   U52150
- EMBL:   AE003852
- PIR:   G82280
- RefSeq:   NP_230423.1
- ProteinModelPortal:   Q56632
- SMR:   Q56632
- GeneID:   2615317
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0774
- TIGR:   VC_0774
- HOGENOM:   HBG750976
- OMA:   NNIVESD
- ProtClustDB:   PRK08220
- BioCyc:   MetaCyc:VC0774-MONOMER
- BioCyc:   VCHO:VC0774-MONOMER
- BRENDA:   1.3.1.28
- GO:   GO:0005488
- InterPro:   IPR002198
- InterPro:   IPR003560
- InterPro:   IPR016040
- InterPro:   IPR020904
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR19410
- PRINTS:   PR01397

Pfam domain/function: PF00106 adh_short

EC number: =1.3.1.28

Molecular weight: Translated: 28094; Mature: 28094

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00061 ADH_SHORT

Important sites: ACT_SITE 158-158 BINDING 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDMSLVNSKVLLVGSARGIGFSVLEHLLQAGAQVMAADCEWQLLLEQSESLLGRYPDQL
CCCCHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCHH
TLKKLDLAEPEAVREQVNQWAEQVAGFDHLVCCAGILHVAPLHEMPMEQVSSIFTVNAFG
HHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHH
VLACMQGVASSMKARQQGSMVIIGSNAANTPRMSIGAYGASKAALHMLVKCIGMELAPYG
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCC
IRCNLVSPGSTRTAMQQQLWTEQYGEAQVIAGDAAQFRLGIPLNKIAEPADIAQAVLFLL
EEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCHHHHCCHHHHHHHHHHHH
SDNAGHITLHDLRVDGGATLDH
CCCCCEEEEEEEEECCCCCCCC
>Mature Secondary Structure
MKDMSLVNSKVLLVGSARGIGFSVLEHLLQAGAQVMAADCEWQLLLEQSESLLGRYPDQL
CCCCHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCHH
TLKKLDLAEPEAVREQVNQWAEQVAGFDHLVCCAGILHVAPLHEMPMEQVSSIFTVNAFG
HHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHH
VLACMQGVASSMKARQQGSMVIIGSNAANTPRMSIGAYGASKAALHMLVKCIGMELAPYG
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCC
IRCNLVSPGSTRTAMQQQLWTEQYGEAQVIAGDAAQFRLGIPLNKIAEPADIAQAVLFLL
EEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCHHHHCCHHHHHHHHHHHH
SDNAGHITLHDLRVDGGATLDH
CCCCCEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371453; 10952301