Click here to switch to the map view.

The map label for this gene is vibB

Identifier: 147674293

GI number: 147674293

Start: 316910

End: 317791

Strand: Direct

Name: vibB

Synonym: VC0395_A0300

Alternate gene names: 147674293

Gene position: 316910-317791 (Clockwise)

Preceding gene: 147673823

Following gene: 147674539

Centisome position: 10.48

GC content: 48.3

Gene sequence:

>882_bases
ATGGCTATTCCTAAAATCGCTTCTTACCCTTTACCCGTATCATTACCGACTAATAAAGTCGATTGGCGCATTGATGCATC
GCGGGCGGTACTGCTTATCCATGATATGCAGGAGTACTTTGTACACTATTTTGACTCGCAGGCAGAACCCATCCCAAGTT
TAATCAAGCATATACAACAGCTTAAAGCTCATGCCAAGCAAGCGGGGATCCCTGTGGTCTATACGGCTCAACCCGCGAAT
CAAGATCCTGCCGAGCGAGCACTATTGAGCGATTTTTGGGGGCCCGGTTTGAGTGAAGAGACGGCGATCATTGCGCCTCT
TGCTCCAGAATCAGGTGATGTTCAGCTCACCAAATGGCGTTATAGCGCGTTTAAAAAGTCGCCGTTATTGGATTGGCTGC
GTGAAACAGGGCGGGATCAGTTAATCATTACGGGTGTCTATGCGCACATTGGCATTCTCTCGACTGCGTTGGATGCCTTT
ATGTTCGATATTCAGCCTTTTGTGATTGGGGATGGCGTGGCAGATTTCTCGCTCAGTGATCACGAATTCAGTCTGCGTTA
TATCAGTGGTCGTACTGGTGCGGTCAAATCCACTCAACAAGCGTGTTTAGAGATTGCCGCCCAACACTCAAAGCTTACTG
GCTTAAGCTTGCGAACCATGCAACACGATGTGGCCGCTGCGCTCAATCTGAGTGTGGATGAAGTGGATGTGCAGGAGAAT
CTTCTGTTTCTCGGTTTAGATTCGATTCGCGCGATTCAGTTGTTGGAAAAATGGAAAGCCCAAGGTGCTGATATTAGCTT
TGCTCAACTGATGGAGCATGTCACTTTGCAGCAGTGGTGGCAGACCATTCAAGCCAATTTACATCAACCTTGCTCTGCGT
GA

Upstream 100 bases:

>100_bases
GAAGATTCGGCCGAATGGCTGCTTCTGCTGCGTCGTGCGATGACATAACAACACTAACAACGCTCAGTTGGCTGAGTATC
AATAAGGATGATGTACTTTC

Downstream 100 bases:

>100_bases
TTGAACGAGTTCCCTTCCTGCTTAACACACGGATTCTAAGTAGGAAGGGAACCGCTTATTCAAGGATTCCCTCTACTCAC
TCATTGTCTTTACATTGATT

Product: vibriobactin-specific isochorismatase

Products: NA

Alternate protein names: 2,3 dihydro-2,3 dihydroxybenzoate synthase; Isochorismate lyase-ArCP

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MAIPKIASYPLPVSLPTNKVDWRIDASRAVLLIHDMQEYFVHYFDSQAEPIPSLIKHIQQLKAHAKQAGIPVVYTAQPAN
QDPAERALLSDFWGPGLSEETAIIAPLAPESGDVQLTKWRYSAFKKSPLLDWLRETGRDQLIITGVYAHIGILSTALDAF
MFDIQPFVIGDGVADFSLSDHEFSLRYISGRTGAVKSTQQACLEIAAQHSKLTGLSLRTMQHDVAAALNLSVDEVDVQEN
LLFLGLDSIRAIQLLEKWKAQGADISFAQLMEHVTLQQWWQTIQANLHQPCSA

Sequences:

>Translated_293_residues
MAIPKIASYPLPVSLPTNKVDWRIDASRAVLLIHDMQEYFVHYFDSQAEPIPSLIKHIQQLKAHAKQAGIPVVYTAQPAN
QDPAERALLSDFWGPGLSEETAIIAPLAPESGDVQLTKWRYSAFKKSPLLDWLRETGRDQLIITGVYAHIGILSTALDAF
MFDIQPFVIGDGVADFSLSDHEFSLRYISGRTGAVKSTQQACLEIAAQHSKLTGLSLRTMQHDVAAALNLSVDEVDVQEN
LLFLGLDSIRAIQLLEKWKAQGADISFAQLMEHVTLQQWWQTIQANLHQPCSA
>Mature_292_residues
AIPKIASYPLPVSLPTNKVDWRIDASRAVLLIHDMQEYFVHYFDSQAEPIPSLIKHIQQLKAHAKQAGIPVVYTAQPANQ
DPAERALLSDFWGPGLSEETAIIAPLAPESGDVQLTKWRYSAFKKSPLLDWLRETGRDQLIITGVYAHIGILSTALDAFM
FDIQPFVIGDGVADFSLSDHEFSLRYISGRTGAVKSTQQACLEIAAQHSKLTGLSLRTMQHDVAAALNLSVDEVDVQENL
LFLGLDSIRAIQLLEKWKAQGADISFAQLMEHVTLQQWWQTIQANLHQPCSA

Specific function: Involved in the biosynthesis of the catechol siderophore vibriobactin. Vibriobactin is a chelating compound involved in transporting iron from the bacterial environment into the cell cytoplasm

COG id: COG1535

COG function: function code Q; Isochorismate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain

Homologues:

Organism=Escherichia coli, GI1786811, Length=289, Percent_Identity=44.2906574394464, Blast_Score=246, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): VIBB_VIBC3 (A5F3F4)

Other databases:

- EMBL:   AF287252
- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F3F4
- SMR:   A5F3F4
- STRING:   A5F3F4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0300
- eggNOG:   COG1535
- HOGENOM:   HBG289118
- OMA:   YASNRCA
- ProtClustDB:   CLSK874104
- BioCyc:   VCHO345073:VC0395_A0300-MONOMER
- InterPro:   IPR009081
- InterPro:   IPR016291
- InterPro:   IPR000868
- InterPro:   IPR006163
- Gene3D:   G3DSA:1.10.1200.10
- Gene3D:   G3DSA:3.40.50.850
- PIRSF:   PIRSF001111
- PRINTS:   PR01398

Pfam domain/function: PF00857 Isochorismatase; PF00550 PP-binding; SSF47336 ACP_like; SSF52499 Iscrsm_hydrolase

EC number: =3.3.2.1

Molecular weight: Translated: 32554; Mature: 32422

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPKIASYPLPVSLPTNKVDWRIDASRAVLLIHDMQEYFVHYFDSQAEPIPSLIKHIQQ
CCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHH
LKAHAKQAGIPVVYTAQPANQDPAERALLSDFWGPGLSEETAIIAPLAPESGDVQLTKWR
HHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEHH
YSAFKKSPLLDWLRETGRDQLIITGVYAHIGILSTALDAFMFDIQPFVIGDGVADFSLSD
HHHHCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
HEFSLRYISGRTGAVKSTQQACLEIAAQHSKLTGLSLRTMQHDVAAALNLSVDEVDVQEN
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHC
LLFLGLDSIRAIQLLEKWKAQGADISFAQLMEHVTLQQWWQTIQANLHQPCSA
EEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AIPKIASYPLPVSLPTNKVDWRIDASRAVLLIHDMQEYFVHYFDSQAEPIPSLIKHIQQ
CCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHH
LKAHAKQAGIPVVYTAQPANQDPAERALLSDFWGPGLSEETAIIAPLAPESGDVQLTKWR
HHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEHH
YSAFKKSPLLDWLRETGRDQLIITGVYAHIGILSTALDAFMFDIQPFVIGDGVADFSLSD
HHHHCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
HEFSLRYISGRTGAVKSTQQACLEIAAQHSKLTGLSLRTMQHDVAAALNLSVDEVDVQEN
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHC
LLFLGLDSIRAIQLLEKWKAQGADISFAQLMEHVTLQQWWQTIQANLHQPCSA
EEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA