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The map label for this gene is hisS

Identifier: 147674318

GI number: 147674318

Start: 302957

End: 304225

Strand: Direct

Name: hisS

Synonym: VC0395_A0289

Alternate gene names: 147674318

Gene position: 302957-304225 (Clockwise)

Preceding gene: 147673394

Following gene: 147674532

Centisome position: 10.02

GC content: 49.65

Gene sequence:

>1269_bases
GTGGCAAAAACTATTCAAGCAATCCGAGGCATGAACGATTGTCTCCCAACCCAGTCTCCACTTTGGCAAAAAGTGGAAGG
CGTGGTGAAAAATGTAATCAGCGCTTACGGTTACAGCGAAGTTCGTATGCCAATCGTTGAGATGACTCATCTATTTAGCC
GCGCCATCGGTGAAGTGACCGATGTGGTGGAAAAAGAGATGTACACCTTTGAAGATCGCAATGGTGATAGCTTAACGCTG
CGACCTGAAGGTACGGCGGGCTGTGTGCGCTCTGGTATCGAAAATGGTTTGCTGTACAACCAAGAGCAACGTTTGTGGTA
CATGGGACCGATGTTCCGTCACGAACGTCCGCAAAAAGGTCGTTACCGTCAATTCCATCAGTGTGGTGTTGAAGTGTTTG
GTTTAGATGGCCCCGATGTGGACGCTGAACTGATCATGATGACGGCACGTCTGTGGCGCGAATTGGGTATTGCACAACAT
GTGCGTTTAGAGCTCAACTCGATTGGCTCTCTAGAGGCTCGCGCTAATTATCGCACCGCCTTGATTGACTATCTTGAGCA
GTACCAAAACGTACTGGATGAAGATTGCAAGCGCCGCATGTACACCAACCCGCTGCGTGTGCTTGATTCGAAGAATCCTG
ATGTACAAGCGATTTTAGGTGATGCCCCTCAGCTCTCTGATTATCTCGATGCTGAATCAAAACAACATTTTGCGGGCTTG
TGTGAACTTCTGGATGCGGCGGGTATCGAATACACGGTAAATCAACGTTTAGTTCGCGGCCTTGATTATTACAACCGCAC
GGTTTTTGAGTGGATCACCGAAAGTCTGGGATCGCAAGGTACCGTTTGTGGCGGCGGCCGCTATGATGGCTTGGTTGAAC
AACTGGGCGGTAAACCAACCCCTGCGGTAGGTTTCGCTATGGGCCTAGAGCGTTTAGTGCTGATGATGGAAACACTCGGT
AATACGGATGTCCGTCGCAGCGTAGATGTGTATATGGTTACTGCAGGTGAAGGCACCATGATGGCGGGAATGAAGCTTGC
GGAACAGTTACGTGAGCAAGTGCCCGGCCTACGTGTGATGACTCACTTCGGTGGCGGCAATTTTAAAAAGCAATTTAAAC
GCGCGGATAAAGTGGGCGCAGCGATTGCCTTGGTTTTGGGTGAAGATGAAGTTGCAGCCCAAACCGTTGTGGTAAAAGAT
TTGGCGGGAGGCGAGCAAAATACTGTTGCCCAAGCTGAAGTAGCTAAACTACTGGCACATTTAGCCTAA

Upstream 100 bases:

>100_bases
GCGAAAATTCGCGCTAAAGCCGCGCGATTGGATGAAAAAAATCGCATCGATATCAAGCACGTAGAACAAGATTAATCTCA
ACGATATAACGGTAAGAATT

Downstream 100 bases:

>100_bases
TTCTCCTGCAGCACGTTTTAAATAAGAGGACAGGAAGTGGAACTCTACGATACTGAAGAACAACAAGTTGAAGCGATCAA
AGATTGGTGGAAAGAGAACG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MAKTIQAIRGMNDCLPTQSPLWQKVEGVVKNVISAYGYSEVRMPIVEMTHLFSRAIGEVTDVVEKEMYTFEDRNGDSLTL
RPEGTAGCVRSGIENGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQCGVEVFGLDGPDVDAELIMMTARLWRELGIAQH
VRLELNSIGSLEARANYRTALIDYLEQYQNVLDEDCKRRMYTNPLRVLDSKNPDVQAILGDAPQLSDYLDAESKQHFAGL
CELLDAAGIEYTVNQRLVRGLDYYNRTVFEWITESLGSQGTVCGGGRYDGLVEQLGGKPTPAVGFAMGLERLVLMMETLG
NTDVRRSVDVYMVTAGEGTMMAGMKLAEQLREQVPGLRVMTHFGGGNFKKQFKRADKVGAAIALVLGEDEVAAQTVVVKD
LAGGEQNTVAQAEVAKLLAHLA

Sequences:

>Translated_422_residues
MAKTIQAIRGMNDCLPTQSPLWQKVEGVVKNVISAYGYSEVRMPIVEMTHLFSRAIGEVTDVVEKEMYTFEDRNGDSLTL
RPEGTAGCVRSGIENGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQCGVEVFGLDGPDVDAELIMMTARLWRELGIAQH
VRLELNSIGSLEARANYRTALIDYLEQYQNVLDEDCKRRMYTNPLRVLDSKNPDVQAILGDAPQLSDYLDAESKQHFAGL
CELLDAAGIEYTVNQRLVRGLDYYNRTVFEWITESLGSQGTVCGGGRYDGLVEQLGGKPTPAVGFAMGLERLVLMMETLG
NTDVRRSVDVYMVTAGEGTMMAGMKLAEQLREQVPGLRVMTHFGGGNFKKQFKRADKVGAAIALVLGEDEVAAQTVVVKD
LAGGEQNTVAQAEVAKLLAHLA
>Mature_421_residues
AKTIQAIRGMNDCLPTQSPLWQKVEGVVKNVISAYGYSEVRMPIVEMTHLFSRAIGEVTDVVEKEMYTFEDRNGDSLTLR
PEGTAGCVRSGIENGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQCGVEVFGLDGPDVDAELIMMTARLWRELGIAQHV
RLELNSIGSLEARANYRTALIDYLEQYQNVLDEDCKRRMYTNPLRVLDSKNPDVQAILGDAPQLSDYLDAESKQHFAGLC
ELLDAAGIEYTVNQRLVRGLDYYNRTVFEWITESLGSQGTVCGGGRYDGLVEQLGGKPTPAVGFAMGLERLVLMMETLGN
TDVRRSVDVYMVTAGEGTMMAGMKLAEQLREQVPGLRVMTHFGGGNFKKQFKRADKVGAAIALVLGEDEVAAQTVVVKDL
AGGEQNTVAQAEVAKLLAHLA

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=469, Percent_Identity=23.0277185501066, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI6996014, Length=458, Percent_Identity=22.2707423580786, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1788861, Length=423, Percent_Identity=74.468085106383, Blast_Score=660, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71993686, Length=451, Percent_Identity=24.1685144124169, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71993693, Length=451, Percent_Identity=24.1685144124169, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24643061, Length=465, Percent_Identity=23.4408602150538, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24643059, Length=480, Percent_Identity=23.5416666666667, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_VIBC3 (A5F3G1)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F3G1
- SMR:   A5F3G1
- STRING:   A5F3G1
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0289
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   VCHO345073:VC0395_A0289-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 46929; Mature: 46798

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTIQAIRGMNDCLPTQSPLWQKVEGVVKNVISAYGYSEVRMPIVEMTHLFSRAIGEVT
CCHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
DVVEKEMYTFEDRNGDSLTLRPEGTAGCVRSGIENGLLYNQEQRLWYMGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQCGVEVFGLDGPDVDAELIMMTARLWRELGIAQHVRLELNSIGSLEARANYRTA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHH
LIDYLEQYQNVLDEDCKRRMYTNPLRVLDSKNPDVQAILGDAPQLSDYLDAESKQHFAGL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEECCCCHHHHHHCCCHHHHHHHH
CELLDAAGIEYTVNQRLVRGLDYYNRTVFEWITESLGSQGTVCGGGRYDGLVEQLGGKPT
HHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCC
PAVGFAMGLERLVLMMETLGNTDVRRSVDVYMVTAGEGTMMAGMKLAEQLREQVPGLRVM
CHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEE
THFGGGNFKKQFKRADKVGAAIALVLGEDEVAAQTVVVKDLAGGEQNTVAQAEVAKLLAH
EECCCCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LA
CC
>Mature Secondary Structure 
AKTIQAIRGMNDCLPTQSPLWQKVEGVVKNVISAYGYSEVRMPIVEMTHLFSRAIGEVT
CHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
DVVEKEMYTFEDRNGDSLTLRPEGTAGCVRSGIENGLLYNQEQRLWYMGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQCGVEVFGLDGPDVDAELIMMTARLWRELGIAQHVRLELNSIGSLEARANYRTA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHH
LIDYLEQYQNVLDEDCKRRMYTNPLRVLDSKNPDVQAILGDAPQLSDYLDAESKQHFAGL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEECCCCHHHHHHCCCHHHHHHHH
CELLDAAGIEYTVNQRLVRGLDYYNRTVFEWITESLGSQGTVCGGGRYDGLVEQLGGKPT
HHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCC
PAVGFAMGLERLVLMMETLGNTDVRRSVDVYMVTAGEGTMMAGMKLAEQLREQVPGLRVM
CHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEE
THFGGGNFKKQFKRADKVGAAIALVLGEDEVAAQTVVVKDLAGGEQNTVAQAEVAKLLAH
EECCCCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA