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The map label for this gene is ispG [H]

Identifier: 147673394

GI number: 147673394

Start: 301801

End: 302931

Strand: Direct

Name: ispG [H]

Synonym: VC0395_A0288

Alternate gene names: 147673394

Gene position: 301801-302931 (Clockwise)

Preceding gene: 147675566

Following gene: 147674318

Centisome position: 9.98

GC content: 50.04

Gene sequence:

>1131_bases
ATGTCTATGCAACATGAGTCTCCTATCAAACGTCGCCCCTCGACACGTATCTATGTGGGCGACGTTCCTATTGGTGATGG
TGCGCCCATCGCCGTACAGTCAATGACCAACACCCGAACCACTGACGTGGCTGCCACGGTTGCGCAGATCAAGTCTTTGG
AAAAAGTGGGGGCGGACATTGTGCGTGTTTCGGTTCCGACTATGGAAGCTGCCGAAGCGTTCAAACTGATCAAACAACAA
GTCTCTGTGCCTTTAGTGGCCGATATCCACTTCGATTATCGTATTGCGCTAAAAGTGGCGGAATACGGTGTGGATTGTCT
ACGCATCAACCCGGGCAATATCGGTAACGAAGCGCGGATTCGTTCGGTTGTGGATTGTGCGCGCGACAAAGGCATTCCGA
TTCGAATCGGGGTCAATGGCGGCTCGCTAGAAAAAGATCTGCAACTGAAATACGGTGAACCGACGCCAGAAGCCTTAGTG
GAATCGGCGATGCGTCATGTGGATATTTTAGATCGCCTCAACTTTGATCAGTTCAAAGTCAGCGTAAAAGCCTCCGATGT
TTTCCTTGCGGTAGACTCCTATCGACTGCTGGCGAAAAAGATTGATCAGCCTTTGCATCTTGGCATTACTGAAGCGGGGG
GCGCGCGTGCTGGTTCAGTGAAGTCAGCCGTAGGCCTAGGTATGCTGCTTGCGGAAGGTATTGGCGATACGCTGCGTATT
TCGCTGGCGGCCGATCCGGTAGAAGAAATCAAAGTTGGTTTTGATATTTTAAAATCGCTGCGCATTCGTTCGCGTGGCAT
TAACTTTATTGCATGCCCAAGCTGTTCACGTCAGGAGTTTGATGTGATTGGCACCGTGAATGCCCTTGAGCAGCGTCTAG
AAGACGTGCTGACACCAATGGATGTATCAATCATTGGCTGTGTGGTAAACGGTCCCGGTGAAGCTGAGGTGTCTCACTTA
GGCTTAGCGGGCAGTAATAAGAAAAGTGCGTTCTATGAAGATGGCGTGCGCCAGAAAGAGCGATTTGATAACGATGATCT
CGTCGCTCAACTGGAAGCGAAAATTCGCGCTAAAGCCGCGCGATTGGATGAAAAAAATCGCATCGATATCAAGCACGTAG
AACAAGATTAA

Upstream 100 bases:

>100_bases
GGTGCACCAGAAGGGGTTTCAATCACATTAGCGAGTGAACCCGTCGACCTTTCTGGGTATACTTCCGGCAAAGTAGCAAG
ATTGACCTTACCTTAGAAAA

Downstream 100 bases:

>100_bases
TCTCAACGATATAACGGTAAGAATTGTGGCAAAAACTATTCAAGCAATCCGAGGCATGAACGATTGTCTCCCAACCCAGT
CTCCACTTTGGCAAAAAGTG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSMQHESPIKRRPSTRIYVGDVPIGDGAPIAVQSMTNTRTTDVAATVAQIKSLEKVGADIVRVSVPTMEAAEAFKLIKQQ
VSVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEARIRSVVDCARDKGIPIRIGVNGGSLEKDLQLKYGEPTPEALV
ESAMRHVDILDRLNFDQFKVSVKASDVFLAVDSYRLLAKKIDQPLHLGITEAGGARAGSVKSAVGLGMLLAEGIGDTLRI
SLAADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVIGTVNALEQRLEDVLTPMDVSIIGCVVNGPGEAEVSHL
GLAGSNKKSAFYEDGVRQKERFDNDDLVAQLEAKIRAKAARLDEKNRIDIKHVEQD

Sequences:

>Translated_376_residues
MSMQHESPIKRRPSTRIYVGDVPIGDGAPIAVQSMTNTRTTDVAATVAQIKSLEKVGADIVRVSVPTMEAAEAFKLIKQQ
VSVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEARIRSVVDCARDKGIPIRIGVNGGSLEKDLQLKYGEPTPEALV
ESAMRHVDILDRLNFDQFKVSVKASDVFLAVDSYRLLAKKIDQPLHLGITEAGGARAGSVKSAVGLGMLLAEGIGDTLRI
SLAADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVIGTVNALEQRLEDVLTPMDVSIIGCVVNGPGEAEVSHL
GLAGSNKKSAFYEDGVRQKERFDNDDLVAQLEAKIRAKAARLDEKNRIDIKHVEQD
>Mature_375_residues
SMQHESPIKRRPSTRIYVGDVPIGDGAPIAVQSMTNTRTTDVAATVAQIKSLEKVGADIVRVSVPTMEAAEAFKLIKQQV
SVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEARIRSVVDCARDKGIPIRIGVNGGSLEKDLQLKYGEPTPEALVE
SAMRHVDILDRLNFDQFKVSVKASDVFLAVDSYRLLAKKIDQPLHLGITEAGGARAGSVKSAVGLGMLLAEGIGDTLRIS
LAADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVIGTVNALEQRLEDVLTPMDVSIIGCVVNGPGEAEVSHLG
LAGSNKKSAFYEDGVRQKERFDNDDLVAQLEAKIRAKAARLDEKNRIDIKHVEQD

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=373, Percent_Identity=85.254691689008, Blast_Score=655, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 40863; Mature: 40732

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMQHESPIKRRPSTRIYVGDVPIGDGAPIAVQSMTNTRTTDVAATVAQIKSLEKVGADI
CCCCCCCCHHCCCCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCE
VRVSVPTMEAAEAFKLIKQQVSVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEARI
EEEECCCHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEECCCCEEEEECCCCCCCHHHH
RSVVDCARDKGIPIRIGVNGGSLEKDLQLKYGEPTPEALVESAMRHVDILDRLNFDQFKV
HHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
SVKASDVFLAVDSYRLLAKKIDQPLHLGITEAGGARAGSVKSAVGLGMLLAEGIGDTLRI
EEEECEEEEEEHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
SLAADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVIGTVNALEQRLEDVLTPM
EEECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHEECHHHHHHHHHHHCCCC
DVSIIGCVVNGPGEAEVSHLGLAGSNKKSAFYEDGVRQKERFDNDDLVAQLEAKIRAKAA
CEEEEEEEECCCCCCCHHEEECCCCCCCCHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHH
RLDEKNRIDIKHVEQD
HCCCCCCCCCEECCCC
>Mature Secondary Structure 
SMQHESPIKRRPSTRIYVGDVPIGDGAPIAVQSMTNTRTTDVAATVAQIKSLEKVGADI
CCCCCCCHHCCCCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCE
VRVSVPTMEAAEAFKLIKQQVSVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEARI
EEEECCCHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEECCCCEEEEECCCCCCCHHHH
RSVVDCARDKGIPIRIGVNGGSLEKDLQLKYGEPTPEALVESAMRHVDILDRLNFDQFKV
HHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
SVKASDVFLAVDSYRLLAKKIDQPLHLGITEAGGARAGSVKSAVGLGMLLAEGIGDTLRI
EEEECEEEEEEHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
SLAADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVIGTVNALEQRLEDVLTPM
EEECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHEECHHHHHHHHHHHCCCC
DVSIIGCVVNGPGEAEVSHLGLAGSNKKSAFYEDGVRQKERFDNDDLVAQLEAKIRAKAA
CEEEEEEEECCCCCCCHHEEECCCCCCCCHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHH
RLDEKNRIDIKHVEQD
HCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]