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The map label for this gene is nifU [H]

Identifier: 147674136

GI number: 147674136

Start: 293164

End: 293547

Strand: Direct

Name: nifU [H]

Synonym: VC0395_A0278

Alternate gene names: 147674136

Gene position: 293164-293547 (Clockwise)

Preceding gene: 147674054

Following gene: 147674050

Centisome position: 9.69

GC content: 47.14

Gene sequence:

>384_bases
ATGGCATACAGCGAAAAGGTCATTGACCATTACGAGAACCCACGTAACGTTGGTTCATTTGATAAAGAAGATCCTTCTGT
AGGTAGCGGCATGGTTGGCGCACCTGCGTGTGGTGATGTAATGCGTTTGCAGATCAAAGTATCACCAGAAGGCATCATCG
AAGATGCAAAATTCAAAACTTACGGCTGTGGTAGCGCAATTGCTTCTAGCTCGCTAGTGACTGAGTGGGTGAAAGGCAAG
AGCATTGATGAAGCGGCAGCGATCAAGAACAGTGAAATCGCCGAAGAGCTCGAATTGCCACCGGTGAAAATTCACTGCTC
AATCCTAGCCGAAGATGCCATCAAAGCCGCTGTTGCGGACTACAAGAAAAAGCATCAACACTAA

Upstream 100 bases:

>100_bases
TCCACTTTGGGACATGTACAAAGACGGCGTAGATCTGAACACGGTTGAGTGGGCACACCACTAATCATTCCAAGGTTATA
AAGGATTCGAGGTAACGAAC

Downstream 100 bases:

>100_bases
TTCGTTTGGGAGCGTCGAGCGCTCCCACCCTTTAATTTATTGAAATCATAAGGTTGAAGTATGGCCGTCACTCTTAGCGA
AACCGCAGCAAGCCGAGTAA

Product: scaffold protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MAYSEKVIDHYENPRNVGSFDKEDPSVGSGMVGAPACGDVMRLQIKVSPEGIIEDAKFKTYGCGSAIASSSLVTEWVKGK
SIDEAAAIKNSEIAEELELPPVKIHCSILAEDAIKAAVADYKKKHQH

Sequences:

>Translated_127_residues
MAYSEKVIDHYENPRNVGSFDKEDPSVGSGMVGAPACGDVMRLQIKVSPEGIIEDAKFKTYGCGSAIASSSLVTEWVKGK
SIDEAAAIKNSEIAEELELPPVKIHCSILAEDAIKAAVADYKKKHQH
>Mature_126_residues
AYSEKVIDHYENPRNVGSFDKEDPSVGSGMVGAPACGDVMRLQIKVSPEGIIEDAKFKTYGCGSAIASSSLVTEWVKGKS
IDEAAAIKNSEIAEELELPPVKIHCSILAEDAIKAAVADYKKKHQH

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=124, Percent_Identity=71.7741935483871, Blast_Score=194, Evalue=1e-50,
Organism=Homo sapiens, GI24307953, Length=124, Percent_Identity=70.9677419354839, Blast_Score=192, Evalue=8e-50,
Organism=Escherichia coli, GI1788878, Length=126, Percent_Identity=87.3015873015873, Blast_Score=230, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17543474, Length=123, Percent_Identity=73.9837398373984, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6325122, Length=125, Percent_Identity=70.4, Blast_Score=188, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6324800, Length=125, Percent_Identity=69.6, Blast_Score=182, Evalue=1e-47,
Organism=Drosophila melanogaster, GI21355597, Length=124, Percent_Identity=75.8064516129032, Blast_Score=203, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011339
- InterPro:   IPR002871 [H]

Pfam domain/function: PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 13662; Mature: 13530

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSEKVIDHYENPRNVGSFDKEDPSVGSGMVGAPACGDVMRLQIKVSPEGIIEDAKFKT
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCC
YGCGSAIASSSLVTEWVKGKSIDEAAAIKNSEIAEELELPPVKIHCSILAEDAIKAAVAD
CCCCHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
YKKKHQH
HHHHCCC
>Mature Secondary Structure 
AYSEKVIDHYENPRNVGSFDKEDPSVGSGMVGAPACGDVMRLQIKVSPEGIIEDAKFKT
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCC
YGCGSAIASSSLVTEWVKGKSIDEAAAIKNSEIAEELELPPVKIHCSILAEDAIKAAVAD
CCCCHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
YKKKHQH
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]