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The map label for this gene is tgt

Identifier: 147674126

GI number: 147674126

Start: 284951

End: 286090

Strand: Direct

Name: tgt

Synonym: VC0395_A0270

Alternate gene names: 147674126

Gene position: 284951-286090 (Clockwise)

Preceding gene: 147675363

Following gene: 147673510

Centisome position: 9.42

GC content: 48.07

Gene sequence:

>1140_bases
GTGAAATTAAAATTTGAACTGAAAAAGAAAAACGGTAATGCCCGTCGTGGTCAATTGATTTTTGAACGCGGCACAGTGCA
AACCCCTGCGTTTATGCCCGTAGGTACTTACGGCACGGTAAAAGGGATGACCCCTGAAGAAGTGAAAGAGACCGGCGCAC
AAATTCTGCTCGGTAACACTTTCCACCTCTGGCTGCGCCCTGGCCAAGAAGTGATGAAAATGCACGGTGACTTGCACGAT
TTCATGAACTGGCAAGGTCCTATCCTGACTGACTCTGGTGGTTTTCAGGTATTCAGCTTAGGCGATATCCGTAAGATCAC
CGAAGAAGGCGTGCATTTCCGCAACCCAGTAAACGGCGACAAAATCTTTATGGACGCCGAGAAATCGATGGAAATCCAAA
AAGATTTGGGTTCAGACATCGTGATGATTTTTGACGAGTGTACGCCATACCCAGCGACCCACGATGAAGCGAAAAAATCG
ATGGAAATGTCTCTGCGTTGGGCAAAACGTTCGCGTGACCACTTCGATAAGCTCGAAAACCCGAACAACCTGTTTGGTAT
CGTACAGGGTGGTGTGTATGAAGATTTGCGTGATGTATCGGTAAAAGGCCTGACGGAAATCGGTTTTGATGGTTATGCTG
TGGGTGGTCTTGCGGTGGGTGAGCCGAAAGAAGACATGCATCGTGTACTGGAACATACCTGTCCACAACTGCCGGAAGAC
AAGCCACGTTATCTGATGGGCGTCGGTAAACCGGAAGACTTGGTGGAAGGTGTGCGTCGTGGTATCGACATGTTTGACTG
TGTGATGCCAACGCGTAACGCACGTAACGGTCACCTGTTTGTGACGGGTGGTGTGATCAAGATCCGTAATGCAGCACATA
AAACCGATACAACACCACTGGATCTGCATTGCGACTGTTACACTTGCAAAAATTATTCGAAGTCATACCTGCATCATTTG
GATCGCTGTAACGAAATCCTCGGTGCACGCTTGAATACCATCCATAACCTGCGTTACTACCAACGCTTGATGGAAAGCAT
TCGTAAAGCGATTGATGAAGACCGTTTTGACCAATTTGTAGCCGAGTTCTACGCGCGTCGTAACCGCGAAGTGCCACCAC
TACAAAAAGACAAAGCCTGA

Upstream 100 bases:

>100_bases
GATGGCAATAAGGCTTGAATTATCGACCTGCTGAGAGCGTAAGTTTGCGCGATTAGGTGGGTAAAAATGGTCAGACTGTT
TCTCTGACATTGGAGGCTTC

Downstream 100 bases:

>100_bases
TTTCGTGCACTGGGTTGGATTTGCGTGGTAAGCCGCTTGAATTCACCCCTGTGCATCCCAATAGAATCAACATTAAACAA
CAATAACTTAGAGGACGTTT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKLKFELKKKNGNARRGQLIFERGTVQTPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFHLWLRPGQEVMKMHGDLHD
FMNWQGPILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGDKIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHDEAKKS
MEMSLRWAKRSRDHFDKLENPNNLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPED
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAAHKTDTTPLDLHCDCYTCKNYSKSYLHHL
DRCNEILGARLNTIHNLRYYQRLMESIRKAIDEDRFDQFVAEFYARRNREVPPLQKDKA

Sequences:

>Translated_379_residues
MKLKFELKKKNGNARRGQLIFERGTVQTPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFHLWLRPGQEVMKMHGDLHD
FMNWQGPILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGDKIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHDEAKKS
MEMSLRWAKRSRDHFDKLENPNNLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPED
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAAHKTDTTPLDLHCDCYTCKNYSKSYLHHL
DRCNEILGARLNTIHNLRYYQRLMESIRKAIDEDRFDQFVAEFYARRNREVPPLQKDKA
>Mature_379_residues
MKLKFELKKKNGNARRGQLIFERGTVQTPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFHLWLRPGQEVMKMHGDLHD
FMNWQGPILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGDKIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHDEAKKS
MEMSLRWAKRSRDHFDKLENPNNLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPED
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAAHKTDTTPLDLHCDCYTCKNYSKSYLHHL
DRCNEILGARLNTIHNLRYYQRLMESIRKAIDEDRFDQFVAEFYARRNREVPPLQKDKA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=363, Percent_Identity=42.9752066115703, Blast_Score=307, Evalue=9e-84,
Organism=Escherichia coli, GI1786607, Length=375, Percent_Identity=80.8, Blast_Score=656, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=41.4364640883978, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI25146502, Length=163, Percent_Identity=33.7423312883436, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920510, Length=361, Percent_Identity=46.2603878116344, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI21355311, Length=256, Percent_Identity=28.515625, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_VIBC3 (A5F3H2)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F3H2
- SMR:   A5F3H2
- STRING:   A5F3H2
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0270
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   VCHO345073:VC0395_A0270-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 43270; Mature: 43270

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: ACT_SITE 91-91 BINDING 92-92

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKFELKKKNGNARRGQLIFERGTVQTPAFMPVGTYGTVKGMTPEEVKETGAQILLGNT
CCEEEEEECCCCCCCCCEEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCE
FHLWLRPGQEVMKMHGDLHDFMNWQGPILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGD
EEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEECCHHHHHHHCCCCCCCCCCCC
KIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHDEAKKSMEMSLRWAKRSRDHFDKLEN
EEEEECHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PNNLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPED
CCCEEEEECCCHHHHHHHCCCCCHHHCCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCC
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAAHKTDTTPL
CCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCE
DLHCDCYTCKNYSKSYLHHLDRCNEILGARLNTIHNLRYYQRLMESIRKAIDEDRFDQFV
EEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
AEFYARRNREVPPLQKDKA
HHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLKFELKKKNGNARRGQLIFERGTVQTPAFMPVGTYGTVKGMTPEEVKETGAQILLGNT
CCEEEEEECCCCCCCCCEEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCE
FHLWLRPGQEVMKMHGDLHDFMNWQGPILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGD
EEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEECCHHHHHHHCCCCCCCCCCCC
KIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHDEAKKSMEMSLRWAKRSRDHFDKLEN
EEEEECHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PNNLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPED
CCCEEEEECCCHHHHHHHCCCCCHHHCCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCC
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAAHKTDTTPL
CCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCE
DLHCDCYTCKNYSKSYLHHLDRCNEILGARLNTIHNLRYYQRLMESIRKAIDEDRFDQFV
EEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
AEFYARRNREVPPLQKDKA
HHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA