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The map label for this gene is iscS

Identifier: 147674054

GI number: 147674054

Start: 291913

End: 293127

Strand: Direct

Name: iscS

Synonym: VC0395_A0277

Alternate gene names: 147674054

Gene position: 291913-293127 (Clockwise)

Preceding gene: 147673230

Following gene: 147674136

Centisome position: 9.65

GC content: 49.3

Gene sequence:

>1215_bases
ATGAAACTGCCTATTTATCTAGATTATTCAGCGACATGCCCGGTTGATCCGCGCGTAGCTGAAAAAATGGTTCAGTACAT
GACGATGGATGGTACCTTCGGTAACCCAGCTTCGCGCTCGCACCGTTATGGCTGGCAGGCAGAAGAAGCGGTAGACACTG
CGCGTGAGCAAATCGCAGCCCTGCTGAATGCAGACCCACGTGAAATTGTGTTCACTTCGGGTGCTACTGAATCTGACAAC
CTCGCGATCAAAGGTGTTGCTCACTTCTATAACAAGCAAGGCAAGCACATCATCACCAGCAAAACCGAACACAAAGCGGT
ACTGGATACCATGCGCCAATTGGAGCGTGAAGGCTTTGAAGTGACTTATCTTGATCCAGAAAGCAATGGCTTGGTTGATC
TGGCTAAGCTTGAAGCCGCGATGCGTGATGACACTATTTTGGTTTCTATCATGCACGTGAACAATGAAATCGGCGTGGTG
CAAGATATCGCAGCGATCGGCGAACTGTGCCGTTCACGTAAAGTGGTGTTCCATGTTGATGCAGCGCAATCCGCGGGTAA
AGTGGCTATTGATGTCCAAGAGATGAAGGTTGATCTGATTTCTCTTTCTGCGCACAAAGCTTACGGCCCGAAAGGTATTG
GTGCTTTGTATGTTCGCCGTAAACCACGTATTCGTCTTGAAGCACAAATGCACGGTGGTGGTCATGAACGTGGTTTCCGT
TCTGGTACTCTGCCGACTCACCAAATTGTGGGTATGGGTGAAGCGTTCCGTATCGCGAAGGAAGAGCTGCAGCAAGATTA
CGATCACGCACTGAAGCTGCGTAATCGTCTGCTTGATGGCATTAAAGATATGGAAGCCGTGACCATTAACGGTGATCTGG
ATCAGCGTGTACCACACAATCTCAACGTCAGTTTTGCGTTTGTAGAAGGTGAATCTCTGCTGATGGCACTGAAAGATCTG
GCTGTATCGTCAGGCAGTGCATGTACTTCAGCCAGCCTTGAGCCTTCTTACGTATTGCGCGCTCTGGGTCTTAACGATGA
ACTGGCACACAGCTCAATTCGCTTCTCTTTTGGTCGTTTCACGACCGAAGCAGAGATTGATTATGCGATCGAACTGATCC
GCGTAGCAGTGGATAAACTGCGTGCGATGTCTCCACTTTGGGACATGTACAAAGACGGCGTAGATCTGAACACGGTTGAG
TGGGCACACCACTAA

Upstream 100 bases:

>100_bases
AAAGGGTTTCGCAAAACAACTCAAACACAGTCACCATTGGTGCTGCGCCTTTTGGTATTAATGTTCGCTCATAGCGGCAA
GGTTTACACTGGAGTAGAAA

Downstream 100 bases:

>100_bases
TCATTCCAAGGTTATAAAGGATTCGAGGTAACGAACATGGCATACAGCGAAAAGGTCATTGACCATTACGAGAACCCACG
TAACGTTGGTTCATTTGATA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAALLNADPREIVFTSGATESDN
LAIKGVAHFYNKQGKHIITSKTEHKAVLDTMRQLEREGFEVTYLDPESNGLVDLAKLEAAMRDDTILVSIMHVNNEIGVV
QDIAAIGELCRSRKVVFHVDAAQSAGKVAIDVQEMKVDLISLSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
SGTLPTHQIVGMGEAFRIAKEELQQDYDHALKLRNRLLDGIKDMEAVTINGDLDQRVPHNLNVSFAFVEGESLLMALKDL
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRFTTEAEIDYAIELIRVAVDKLRAMSPLWDMYKDGVDLNTVE
WAHH

Sequences:

>Translated_404_residues
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAALLNADPREIVFTSGATESDN
LAIKGVAHFYNKQGKHIITSKTEHKAVLDTMRQLEREGFEVTYLDPESNGLVDLAKLEAAMRDDTILVSIMHVNNEIGVV
QDIAAIGELCRSRKVVFHVDAAQSAGKVAIDVQEMKVDLISLSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
SGTLPTHQIVGMGEAFRIAKEELQQDYDHALKLRNRLLDGIKDMEAVTINGDLDQRVPHNLNVSFAFVEGESLLMALKDL
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRFTTEAEIDYAIELIRVAVDKLRAMSPLWDMYKDGVDLNTVE
WAHH
>Mature_404_residues
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAALLNADPREIVFTSGATESDN
LAIKGVAHFYNKQGKHIITSKTEHKAVLDTMRQLEREGFEVTYLDPESNGLVDLAKLEAAMRDDTILVSIMHVNNEIGVV
QDIAAIGELCRSRKVVFHVDAAQSAGKVAIDVQEMKVDLISLSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
SGTLPTHQIVGMGEAFRIAKEELQQDYDHALKLRNRLLDGIKDMEAVTINGDLDQRVPHNLNVSFAFVEGESLLMALKDL
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRFTTEAEIDYAIELIRVAVDKLRAMSPLWDMYKDGVDLNTVE
WAHH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=58.4577114427861, Blast_Score=512, Evalue=1e-145,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=32.3671497584541, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=79.2079207920792, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI1789175, Length=397, Percent_Identity=26.1964735516373, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787970, Length=348, Percent_Identity=25.8620689655172, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=59.5533498759305, Blast_Score=499, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17533177, Length=335, Percent_Identity=34.3283582089552, Blast_Score=166, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=57.356608478803, Blast_Score=500, Evalue=1e-142,
Organism=Drosophila melanogaster, GI20129463, Length=402, Percent_Identity=58.2089552238806, Blast_Score=488, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCS_VIBC3 (A5F3G4)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F3G4
- SMR:   A5F3G4
- STRING:   A5F3G4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0277
- eggNOG:   COG1104
- HOGENOM:   HBG635316
- OMA:   DMSPLWD
- ProtClustDB:   PRK14012
- BioCyc:   VCHO345073:VC0395_A0277-MONOMER
- HAMAP:   MF_00331
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572
- TIGRFAMs:   TIGR02006

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 44810; Mature: 44810

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAA
CCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LLNADPREIVFTSGATESDNLAIKGVAHFYNKQGKHIITSKTEHKAVLDTMRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLDPESNGLVDLAKLEAAMRDDTILVSIMHVNNEIGVVQDIAAIGELCRSRKVVFHVD
EEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEE
AAQSAGKVAIDVQEMKVDLISLSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
CCCCCCCEEEEHHHHEEEEEEEECCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCC
SGTLPTHQIVGMGEAFRIAKEELQQDYDHALKLRNRLLDGIKDMEAVTINGDLDQRVPHN
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCC
LNVSFAFVEGESLLMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRF
CCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEECCCC
TTEAEIDYAIELIRVAVDKLRAMSPLWDMYKDGVDLNTVEWAHH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAA
CCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LLNADPREIVFTSGATESDNLAIKGVAHFYNKQGKHIITSKTEHKAVLDTMRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLDPESNGLVDLAKLEAAMRDDTILVSIMHVNNEIGVVQDIAAIGELCRSRKVVFHVD
EEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEE
AAQSAGKVAIDVQEMKVDLISLSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
CCCCCCCEEEEHHHHEEEEEEEECCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCC
SGTLPTHQIVGMGEAFRIAKEELQQDYDHALKLRNRLLDGIKDMEAVTINGDLDQRVPHN
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCC
LNVSFAFVEGESLLMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRF
CCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEECCCC
TTEAEIDYAIELIRVAVDKLRAMSPLWDMYKDGVDLNTVEWAHH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA