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The map label for this gene is apbE [H]

Identifier: 147673710

GI number: 147673710

Start: 1997115

End: 1998218

Strand: Reverse

Name: apbE [H]

Synonym: VC0395_A1878

Alternate gene names: 147673710

Gene position: 1998218-1997115 (Counterclockwise)

Preceding gene: 147675137

Following gene: 147673696

Centisome position: 66.08

GC content: 46.01

Gene sequence:

>1104_bases
TTGTTTTTTACAAATTGGATATTGAAAGTGTCGATGGTTATCTGGTTTAGGGTATCGGTATTTCCCATATCAATTTTTAA
CTATGAAAGGAGTAAACAAGTGAGAAATTGGCTTGTTGCATTAGCTTCTCTTCTCTTGCTGGCTGGCTGTGAAAAGCCCG
CAGAGCAGGTACATCTCAGTGGCCCAACCATGGGAACCACTTACAACATCAAATACATTCAACAGCCGGGAATTGCGGAT
TCGAAAACACTGCAAACCGAGATTGATCGACTCCTTGAAGAAGTGAACGATCAAATGTCGACTTACCGTAAAGACTCTGA
GCTCAGCCGTTTTAACCAGCATACCAGTAGCGAACCTTTTGCCGTTTCTACACAAACGTTAACCGTGGTGAAAGAGGCGA
TTCGTTTGAACGGTCTTACTGAAGGTGCTTTAGATGTGACCGTCGGACCTTTGGTTAATCTGTGGGGATTTGGCCCTGAA
GCCCGTCCTGATGTGGTACCAACCGATGAAGAGCTGAATGCGCGTCGTGCTATTACTGGCATTGAGCATCTGACGATTGA
AGGGAATACGCTGAGTAAAGATATTCCTGAACTGTATGTCGACTTGTCTACGATTGCCAAAGGTTGGGGAGTAGATGTGG
TCGCCGATTACCTTCAATCTCAAGGTATCGAAAACTACATGGTAGAAATTGGCGGAGAAATCCGTTTGAAAGGACTGAAC
CGTGATGGTGTTCCCTGGCGTATTGCGATAGAAAAGCCAAGTGTTGATCAGCGTAGTGTACAAGAGATCATCGAACCGGG
TGATTATGCGATCGCAACCTCCGGTGATTACCGTAACTACTTTGAGCAAGATGGCGTTCGTTACTCGCATATCATCGATC
CAACTACCGGACGCCCAATCAATAACCGCGTGGTCTCCGTGACGGTACTGGATAAGTCTTGTATGACGGCGGATGGCTTA
GCGACAGGCTTGATGGTCATGGGCGAAGAGCGTGGTATGGCTGTCGCAGAAGCCAACCAGATCCCGGTTTTAATGATTGT
GAAAACGGATGATGGCTTTAAAGAGTACGCATCAAGCAGCTTTAAACCGTTTTTAAGTAAGTAA

Upstream 100 bases:

>100_bases
AAAAACCTAGGTGTAGAAGAGGAAAACATCCTACTGGATGACTTCGGTGGTTAATCGATAACTCGCTACACTGCGATGGC
TGGCTCGTTCGAGTCAGCCA

Downstream 100 bases:

>100_bases
TAGTGAGCATAAAACAATGAACACCTTTTTGATTACTTTTGGTTTCTTTCTGGCCGTGATTGCCGCCATGGCCGTTGGTT
ACATTTTCCAACGTAAAGTG

Product: thiamin biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MFFTNWILKVSMVIWFRVSVFPISIFNYERSKQVRNWLVALASLLLLAGCEKPAEQVHLSGPTMGTTYNIKYIQQPGIAD
SKTLQTEIDRLLEEVNDQMSTYRKDSELSRFNQHTSSEPFAVSTQTLTVVKEAIRLNGLTEGALDVTVGPLVNLWGFGPE
ARPDVVPTDEELNARRAITGIEHLTIEGNTLSKDIPELYVDLSTIAKGWGVDVVADYLQSQGIENYMVEIGGEIRLKGLN
RDGVPWRIAIEKPSVDQRSVQEIIEPGDYAIATSGDYRNYFEQDGVRYSHIIDPTTGRPINNRVVSVTVLDKSCMTADGL
ATGLMVMGEERGMAVAEANQIPVLMIVKTDDGFKEYASSSFKPFLSK

Sequences:

>Translated_367_residues
MFFTNWILKVSMVIWFRVSVFPISIFNYERSKQVRNWLVALASLLLLAGCEKPAEQVHLSGPTMGTTYNIKYIQQPGIAD
SKTLQTEIDRLLEEVNDQMSTYRKDSELSRFNQHTSSEPFAVSTQTLTVVKEAIRLNGLTEGALDVTVGPLVNLWGFGPE
ARPDVVPTDEELNARRAITGIEHLTIEGNTLSKDIPELYVDLSTIAKGWGVDVVADYLQSQGIENYMVEIGGEIRLKGLN
RDGVPWRIAIEKPSVDQRSVQEIIEPGDYAIATSGDYRNYFEQDGVRYSHIIDPTTGRPINNRVVSVTVLDKSCMTADGL
ATGLMVMGEERGMAVAEANQIPVLMIVKTDDGFKEYASSSFKPFLSK
>Mature_367_residues
MFFTNWILKVSMVIWFRVSVFPISIFNYERSKQVRNWLVALASLLLLAGCEKPAEQVHLSGPTMGTTYNIKYIQQPGIAD
SKTLQTEIDRLLEEVNDQMSTYRKDSELSRFNQHTSSEPFAVSTQTLTVVKEAIRLNGLTEGALDVTVGPLVNLWGFGPE
ARPDVVPTDEELNARRAITGIEHLTIEGNTLSKDIPELYVDLSTIAKGWGVDVVADYLQSQGIENYMVEIGGEIRLKGLN
RDGVPWRIAIEKPSVDQRSVQEIIEPGDYAIATSGDYRNYFEQDGVRYSHIIDPTTGRPINNRVVSVTVLDKSCMTADGL
ATGLMVMGEERGMAVAEANQIPVLMIVKTDDGFKEYASSSFKPFLSK

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=339, Percent_Identity=43.0678466076696, Blast_Score=284, Evalue=6e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 40849; Mature: 40849

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFTNWILKVSMVIWFRVSVFPISIFNYERSKQVRNWLVALASLLLLAGCEKPAEQVHLS
CCHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEC
GPTMGTTYNIKYIQQPGIADSKTLQTEIDRLLEEVNDQMSTYRKDSELSRFNQHTSSEPF
CCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCE
AVSTQTLTVVKEAIRLNGLTEGALDVTVGPLVNLWGFGPEARPDVVPTDEELNARRAITG
EEEHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCHHHCHHHHHCC
IEHLTIEGNTLSKDIPELYVDLSTIAKGWGVDVVADYLQSQGIENYMVEIGGEIRLKGLN
CEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEHEEECCEEEEEECC
RDGVPWRIAIEKPSVDQRSVQEIIEPGDYAIATSGDYRNYFEQDGVRYSHIIDPTTGRPI
CCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHCCCEEEEEECCCCCCCC
NNRVVSVTVLDKSCMTADGLATGLMVMGEERGMAVAEANQIPVLMIVKTDDGFKEYASSS
CCEEEEEEEECCCCCCCCHHHHHHEEEECCCCCEEEECCCCCEEEEEECCCCHHHHHCCC
FKPFLSK
CCHHHCC
>Mature Secondary Structure
MFFTNWILKVSMVIWFRVSVFPISIFNYERSKQVRNWLVALASLLLLAGCEKPAEQVHLS
CCHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEC
GPTMGTTYNIKYIQQPGIADSKTLQTEIDRLLEEVNDQMSTYRKDSELSRFNQHTSSEPF
CCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCE
AVSTQTLTVVKEAIRLNGLTEGALDVTVGPLVNLWGFGPEARPDVVPTDEELNARRAITG
EEEHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCHHHCHHHHHCC
IEHLTIEGNTLSKDIPELYVDLSTIAKGWGVDVVADYLQSQGIENYMVEIGGEIRLKGLN
CEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEHEEECCEEEEEECC
RDGVPWRIAIEKPSVDQRSVQEIIEPGDYAIATSGDYRNYFEQDGVRYSHIIDPTTGRPI
CCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHCCCEEEEEECCCCCCCC
NNRVVSVTVLDKSCMTADGLATGLMVMGEERGMAVAEANQIPVLMIVKTDDGFKEYASSS
CCEEEEEEEECCCCCCCCHHHHHHEEEECCCCCEEEECCCCCEEEEEECCCCHHHHHCCC
FKPFLSK
CCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]