Click here to switch to the map view.

The map label for this gene is nqrF

Identifier: 147675137

GI number: 147675137

Start: 1998265

End: 1999491

Strand: Reverse

Name: nqrF

Synonym: VC0395_A1879

Alternate gene names: 147675137

Gene position: 1999491-1998265 (Counterclockwise)

Preceding gene: 147674521

Following gene: 147673710

Centisome position: 66.12

GC content: 46.94

Gene sequence:

>1227_bases
ATGTCTACTATTATTTTTGGTGTAGTGATGTTTACCCTGATTATACTGGCTTTGGTTCTGGTGATTCTGTTCGCCAAATC
CAAGCTAGTACCAACAGGTGACATTACAATTTCTATTAATGGCGATCCTGAAAAAGCGATCGTAACCCAACCGGGTGGTA
AGTTGCTGACTGCACTGGCAGGCGCTGGTGTATTCGTATCTTCAGCTTGTGGTGGTGGTGGTTCATGTGGCCAATGCCGT
GTAAAAATCAAATCAGGTGGCGGTGATATTCTGCCGACAGAACTGGATCACATCAGCAAAGGCGAAGCCCGTGAAGGTGA
GCGTTTGGCGTGTCAGGTTGCTGTAAAAGCAGATATGGATCTGGAACTGCCAGAAGAGATCTTTGGCGTGAAGAAGTGGG
AATGTACGGTTATCTCTAACGATAACAAAGCCACTTTCATCAAAGAGCTGAAGCTTGCGATTCCTGATGGTGAATCAGTA
CCATTCCGTGCGGGTGGTTACATTCAGATTGAAGCGCCTGCGCACCATGTTAAGTACGCCGATTTTGATGTACCTGAGAA
ATACCGTGGTGACTGGGACAAATTCAATCTGTTCCGTTATGAGTCTAAAGTGGATGAGCCAATCATCCGTGCATACTCAA
TGGCGAACTACCCTGAAGAGTTTGGCATCATCATGCTGAACGTGCGTATCGCGACTCCGCCGCCAAATAATCCAAATGTG
CCACCAGGCCAAATGTCTTCTTACATCTGGTCACTGAAAGCCGGTGATAAATGTACGATTTCTGGTCCGTTCGGTGAGTT
CTTCGCGAAAGACACGGATGCAGAGATGGTATTTATCGGTGGTGGTGCGGGTATGGCGCCAATGCGTTCGCATATCTTTG
ACCAACTGAAGCGTCTGAAGTCTAAGCGTAAGATGTCTTATTGGTACGGTGCGCGTTCTAAGCGTGAAATGTTCTACGTG
GAAGATTTCGATGGCCTAGCGGCTGAGAATGACAACTTCGTATGGCACTGTGCTCTGTCAGATCCTCAACCAGAAGATAA
CTGGACAGGCTACACCGGTTTCATCCATAACGTACTGTATGAAAACTATCTGAAGGATCACGAAGCTCCGGAAGATTGTG
AATACTACATGTGTGGACCTCCGATGATGAACGCGGCAGTGATCAACATGCTGAAAAACCTAGGTGTAGAAGAGGAAAAC
ATCCTACTGGATGACTTCGGTGGTTAA

Upstream 100 bases:

>100_bases
TATTACCTTTATCACGGCTGGCTTGATGGCGTTGGGCTTCATGTCTTTCTCTGGTGTTCAACTGTAAGTCGGGTAAACCG
CAGCAATAAAGGAATAGTTA

Downstream 100 bases:

>100_bases
TCGATAACTCGCTACACTGCGATGGCTGGCTCGTTCGAGTCAGCCATTGTTTTTTACAAATTGGATATTGAAAGTGTCGA
TGGTTATCTGGTTTAGGGTA

Product: Na(+)-translocating NADH-quinone reductase subunit F

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSTIIFGVVMFTLIILALVLVILFAKSKLVPTGDITISINGDPEKAIVTQPGGKLLTALAGAGVFVSSACGGGGSCGQCR
VKIKSGGGDILPTELDHISKGEAREGERLACQVAVKADMDLELPEEIFGVKKWECTVISNDNKATFIKELKLAIPDGESV
PFRAGGYIQIEAPAHHVKYADFDVPEKYRGDWDKFNLFRYESKVDEPIIRAYSMANYPEEFGIIMLNVRIATPPPNNPNV
PPGQMSSYIWSLKAGDKCTISGPFGEFFAKDTDAEMVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSYWYGARSKREMFYV
EDFDGLAAENDNFVWHCALSDPQPEDNWTGYTGFIHNVLYENYLKDHEAPEDCEYYMCGPPMMNAAVINMLKNLGVEEEN
ILLDDFGG

Sequences:

>Translated_408_residues
MSTIIFGVVMFTLIILALVLVILFAKSKLVPTGDITISINGDPEKAIVTQPGGKLLTALAGAGVFVSSACGGGGSCGQCR
VKIKSGGGDILPTELDHISKGEAREGERLACQVAVKADMDLELPEEIFGVKKWECTVISNDNKATFIKELKLAIPDGESV
PFRAGGYIQIEAPAHHVKYADFDVPEKYRGDWDKFNLFRYESKVDEPIIRAYSMANYPEEFGIIMLNVRIATPPPNNPNV
PPGQMSSYIWSLKAGDKCTISGPFGEFFAKDTDAEMVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSYWYGARSKREMFYV
EDFDGLAAENDNFVWHCALSDPQPEDNWTGYTGFIHNVLYENYLKDHEAPEDCEYYMCGPPMMNAAVINMLKNLGVEEEN
ILLDDFGG
>Mature_407_residues
STIIFGVVMFTLIILALVLVILFAKSKLVPTGDITISINGDPEKAIVTQPGGKLLTALAGAGVFVSSACGGGGSCGQCRV
KIKSGGGDILPTELDHISKGEAREGERLACQVAVKADMDLELPEEIFGVKKWECTVISNDNKATFIKELKLAIPDGESVP
FRAGGYIQIEAPAHHVKYADFDVPEKYRGDWDKFNLFRYESKVDEPIIRAYSMANYPEEFGIIMLNVRIATPPPNNPNVP
PGQMSSYIWSLKAGDKCTISGPFGEFFAKDTDAEMVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSYWYGARSKREMFYVE
DFDGLAAENDNFVWHCALSDPQPEDNWTGYTGFIHNVLYENYLKDHEAPEDCEYYMCGPPMMNAAVINMLKNLGVEEENI
LLDDFGG

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

Organism=Saccharomyces cerevisiae, GI6323552, Length=191, Percent_Identity=26.7015706806283, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323510, Length=154, Percent_Identity=25.3246753246753, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NQRF_VIBC3 (A5F5Y4)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F5Y4
- SMR:   A5F5Y4
- STRING:   A5F5Y4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1879
- eggNOG:   COG2871
- HOGENOM:   HBG680293
- OMA:   VQLECPP
- ProtClustDB:   PRK05464
- BioCyc:   VCHO345073:VC0395_A1879-MONOMER
- HAMAP:   MF_00430
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938
- Gene3D:   G3DSA:3.10.20.30
- TIGRFAMs:   TIGR01941

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1; SSF54292 Ferredoxin; SSF63380 Riboflavin_synthase_like_b-brl

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 45067; Mature: 44936

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00197 2FE2S_FER_1; PS51085 2FE2S_FER_2; PS51384 FAD_FR

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x19b0b744)-;

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIIFGVVMFTLIILALVLVILFAKSKLVPTGDITISINGDPEKAIVTQPGGKLLTALA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHH
GAGVFVSSACGGGGSCGQCRVKIKSGGGDILPTELDHISKGEAREGERLACQVAVKADMD
CCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCCEEEEEEEEECCCC
LELPEEIFGVKKWECTVISNDNKATFIKELKLAIPDGESVPFRAGGYIQIEAPAHHVKYA
CCCCHHHHCCCEEEEEEEECCCCEEEEEEEEEECCCCCCCCEECCCEEEEECCCCEEEEC
DFDVPEKYRGDWDKFNLFRYESKVDEPIIRAYSMANYPEEFGIIMLNVRIATPPPNNPNV
CCCCCHHHCCCCCCEEEEEEHHHCCHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCC
PPGQMSSYIWSLKAGDKCTISGPFGEFFAKDTDAEMVFIGGGAGMAPMRSHIFDQLKRLK
CCCHHHHHEEEECCCCEEEEECCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
SKRKMSYWYGARSKREMFYVEDFDGLAAENDNFVWHCALSDPQPEDNWTGYTGFIHNVLY
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHH
ENYLKDHEAPEDCEYYMCGPPMMNAAVINMLKNLGVEEENILLDDFGG
HHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCH
>Mature Secondary Structure 
STIIFGVVMFTLIILALVLVILFAKSKLVPTGDITISINGDPEKAIVTQPGGKLLTALA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHH
GAGVFVSSACGGGGSCGQCRVKIKSGGGDILPTELDHISKGEAREGERLACQVAVKADMD
CCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCCEEEEEEEEECCCC
LELPEEIFGVKKWECTVISNDNKATFIKELKLAIPDGESVPFRAGGYIQIEAPAHHVKYA
CCCCHHHHCCCEEEEEEEECCCCEEEEEEEEEECCCCCCCCEECCCEEEEECCCCEEEEC
DFDVPEKYRGDWDKFNLFRYESKVDEPIIRAYSMANYPEEFGIIMLNVRIATPPPNNPNV
CCCCCHHHCCCCCCEEEEEEHHHCCHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCC
PPGQMSSYIWSLKAGDKCTISGPFGEFFAKDTDAEMVFIGGGAGMAPMRSHIFDQLKRLK
CCCHHHHHEEEECCCCEEEEECCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
SKRKMSYWYGARSKREMFYVEDFDGLAAENDNFVWHCALSDPQPEDNWTGYTGFIHNVLY
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHH
ENYLKDHEAPEDCEYYMCGPPMMNAAVINMLKNLGVEEENILLDDFGG
HHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA