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The map label for this gene is fusA-2 [H]

Identifier: 147673153

GI number: 147673153

Start: 2048626

End: 2050713

Strand: Reverse

Name: fusA-2 [H]

Synonym: VC0395_A1921

Alternate gene names: 147673153

Gene position: 2050713-2048626 (Counterclockwise)

Preceding gene: 147673564

Following gene: 147673874

Centisome position: 67.81

GC content: 47.17

Gene sequence:

>2088_bases
ATGGCAGATTTATCATTATACAGAAACATTGGTATCTTCGCGCACGTTGACGCGGGTAAGACCACTACCACTGAGCGTAT
CCTGAAGCTGACTGGTAAAATCCACCGTCTGGGCGAAGTACATGACGGCGCTTCAACGATGGACTTCATGGAACAAGAAG
CTGAGCGTGGTATCACTATCCAGTCTGCTGCGACTACCTGTTTCTGGAAAGGTCACCGTTTTAACGTAATCGACACCCCG
GGACACGTTGACTTCACCGTTGAAGTATACCGTTCTCTGAAAGTTCTTGACGGTGGTATCGGTGTATTCTGTGGTTCTGG
CGGTGTTGAACCTCAGTCAGAAACTAACTGGCGTTACGCGAACGAATCAGAAGTATCTCGTCTGATCTTCGTTAACAAAC
TAGACCGTATGGGTGCTGACTTCTTCCGCGTAGTTGAGCAAGTGAAGAAAGTTCTTGGCGCAAACCCACTGGTTATGACT
CTACCTATTGGCCGCGAAGATGAATTCGTTGGTGTGGTTGATGTACTAACTCGTCAAGCGTACGTATGGGATGACTCAGG
TCTTCCAGAAAACTTCGAAGTGAAAGAAGTTCCAGCAGACATGGTTGATCAGGTAGAAGAATACCGCGAAATGATGATCG
AAACTGCTGTTGAGCAAGATGACGAGCTGATGATGGCTTACATGGAAGGCGAAGAGCCAACTGTTGAGCAAATCAAAGCA
TGTATCCGTAAAGGTACACGTGATCTCGCGTTCTTCCCAACTTTCTGTGGTTCTGCGTTTAAAAACAAAGGTATGCAGCT
AGTTCTGGATGCAGTTGTTGACTACCTGCCTTCTCCAACAGAAGTTGAGCCACAACCACTGACTGATCCAGCAACGGGTG
AGCCTACTGGTGAAGTAGCAACAGTATCTGTTGATGCACCACTAAAAGCGCTAGCGTTCAAGATCATGGATGACCGTTTT
GGTGCCCTGACCTTCGTTCGTCTCTACTCAGGTAAGATTAAGAAGGGTGATACCATTCTTAACAGCGCAACAGGTAAAAC
TGAGCGTATCGGCCGTATGGTTGAGATGCACGCAAACGACCGTAACGAAGTGGAATCTGCACAAGCAGGTGACATCATCG
CGATCGTTGGTATGAAGAACGTTCAAACAGGTCACACTCTATGTGATCCTAAGCACGAATGTACTCTTGAGCCAATGATT
TTCCCAACACCAGTTATCTCTATCGCTGTTAAGCCTAAAGATAAGAACGGTTCTGAGAAAATGGGTATCGCGATTGGTAA
GATGGTTGCAGAAGATCCATCATTCCAAGTGGAGACTGACGAAGATTCTGGCGAAACCATCCTGAAAGGTATGGGTGAGC
TGCATCTAGACATCAAAGTAGACATCCTTAAGCGTACTTACGGCGTTGAGCTGGAAGTGGGTGCTCCTCAAGTTGCTTAC
CGTGAAACTATCACTAAAGCAGTTGAAGATAGCTACACGCATAAGAAACAGTCTGGTGGTTCAGGTCAGTTCGGTAAGAT
CGACTACCGTATCCGTCCAGGAGAGCAAAACTCTGGCTTCACCTTCAAGTCAACCGTTGTTGGTGGTAACGTTCCTAAGG
AATTCTGGCCAGCAGTTGAGAAAGGCTTCAAGTCTATGATGGATACCGGTACTCTTGCTGGCTTCCCTGTACTTGACGTT
GAAGTTGAACTGTTCGACGGTGGCTTCCACGCAGTTGACTCATCTGCAATCGCATTTGAAATCGCAGCGAAAGGCGCATT
CCGTCAGTCAATTCCAAAAGCGGCTCCTCAGCTTCTTGAGCCAATCATGAAAGTGGATGTGTTCACTCCGGAAGATCACG
TGGGTGACGTTATCGGTGACTTGAACCGTCGTCGTGGCATGATCAAAGACCAAGAAATGGGTCTTACAGGCGTACGCGTT
AAAGCAGACGTACCACTATCTGAGATGTTCGGCTACATCGGTTCTCTACGTACAATGACTTCTGGTCGTGGTCAGTTCTC
TATGGAGTTCTCTCACTACGCACCATGTCCAAACAACGTAGCTGAGCAAGTTATTGCTGAAGTTAAAGAGCGTAATGCGA
AGAAGTAA

Upstream 100 bases:

>100_bases
AGGGCATATTTTTTTTCAGCAAGCGCATGCAGGGGTATCAGTCTTGACAGATTGTGATATACTCTGCGCGCACTTTATAC
CTTATTAACAGAGTAAGACA

Downstream 100 bases:

>100_bases
TTCTTTGTTTTAAAGCTTAAAAAGCCCCGTAAGTGAAAGCTTACGGGGCTTTTGTCTTCCTGCCAGCGAGGACGGCTCGA
CAAGCAAGGCCGTCCGCTTA

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G 2 [H]

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MADLSLYRNIGIFAHVDAGKTTTTERILKLTGKIHRLGEVHDGASTMDFMEQEAERGITIQSAATTCFWKGHRFNVIDTP
GHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYANESEVSRLIFVNKLDRMGADFFRVVEQVKKVLGANPLVMT
LPIGREDEFVGVVDVLTRQAYVWDDSGLPENFEVKEVPADMVDQVEEYREMMIETAVEQDDELMMAYMEGEEPTVEQIKA
CIRKGTRDLAFFPTFCGSAFKNKGMQLVLDAVVDYLPSPTEVEPQPLTDPATGEPTGEVATVSVDAPLKALAFKIMDDRF
GALTFVRLYSGKIKKGDTILNSATGKTERIGRMVEMHANDRNEVESAQAGDIIAIVGMKNVQTGHTLCDPKHECTLEPMI
FPTPVISIAVKPKDKNGSEKMGIAIGKMVAEDPSFQVETDEDSGETILKGMGELHLDIKVDILKRTYGVELEVGAPQVAY
RETITKAVEDSYTHKKQSGGSGQFGKIDYRIRPGEQNSGFTFKSTVVGGNVPKEFWPAVEKGFKSMMDTGTLAGFPVLDV
EVELFDGGFHAVDSSAIAFEIAAKGAFRQSIPKAAPQLLEPIMKVDVFTPEDHVGDVIGDLNRRRGMIKDQEMGLTGVRV
KADVPLSEMFGYIGSLRTMTSGRGQFSMEFSHYAPCPNNVAEQVIAEVKERNAKK

Sequences:

>Translated_695_residues
MADLSLYRNIGIFAHVDAGKTTTTERILKLTGKIHRLGEVHDGASTMDFMEQEAERGITIQSAATTCFWKGHRFNVIDTP
GHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYANESEVSRLIFVNKLDRMGADFFRVVEQVKKVLGANPLVMT
LPIGREDEFVGVVDVLTRQAYVWDDSGLPENFEVKEVPADMVDQVEEYREMMIETAVEQDDELMMAYMEGEEPTVEQIKA
CIRKGTRDLAFFPTFCGSAFKNKGMQLVLDAVVDYLPSPTEVEPQPLTDPATGEPTGEVATVSVDAPLKALAFKIMDDRF
GALTFVRLYSGKIKKGDTILNSATGKTERIGRMVEMHANDRNEVESAQAGDIIAIVGMKNVQTGHTLCDPKHECTLEPMI
FPTPVISIAVKPKDKNGSEKMGIAIGKMVAEDPSFQVETDEDSGETILKGMGELHLDIKVDILKRTYGVELEVGAPQVAY
RETITKAVEDSYTHKKQSGGSGQFGKIDYRIRPGEQNSGFTFKSTVVGGNVPKEFWPAVEKGFKSMMDTGTLAGFPVLDV
EVELFDGGFHAVDSSAIAFEIAAKGAFRQSIPKAAPQLLEPIMKVDVFTPEDHVGDVIGDLNRRRGMIKDQEMGLTGVRV
KADVPLSEMFGYIGSLRTMTSGRGQFSMEFSHYAPCPNNVAEQVIAEVKERNAKK
>Mature_694_residues
ADLSLYRNIGIFAHVDAGKTTTTERILKLTGKIHRLGEVHDGASTMDFMEQEAERGITIQSAATTCFWKGHRFNVIDTPG
HVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYANESEVSRLIFVNKLDRMGADFFRVVEQVKKVLGANPLVMTL
PIGREDEFVGVVDVLTRQAYVWDDSGLPENFEVKEVPADMVDQVEEYREMMIETAVEQDDELMMAYMEGEEPTVEQIKAC
IRKGTRDLAFFPTFCGSAFKNKGMQLVLDAVVDYLPSPTEVEPQPLTDPATGEPTGEVATVSVDAPLKALAFKIMDDRFG
ALTFVRLYSGKIKKGDTILNSATGKTERIGRMVEMHANDRNEVESAQAGDIIAIVGMKNVQTGHTLCDPKHECTLEPMIF
PTPVISIAVKPKDKNGSEKMGIAIGKMVAEDPSFQVETDEDSGETILKGMGELHLDIKVDILKRTYGVELEVGAPQVAYR
ETITKAVEDSYTHKKQSGGSGQFGKIDYRIRPGEQNSGFTFKSTVVGGNVPKEFWPAVEKGFKSMMDTGTLAGFPVLDVE
VELFDGGFHAVDSSAIAFEIAAKGAFRQSIPKAAPQLLEPIMKVDVFTPEDHVGDVIGDLNRRRGMIKDQEMGLTGVRVK
ADVPLSEMFGYIGSLRTMTSGRGQFSMEFSHYAPCPNNVAEQVIAEVKERNAKK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=693, Percent_Identity=43.2900432900433, Blast_Score=550, Evalue=1e-156,
Organism=Homo sapiens, GI19923640, Length=706, Percent_Identity=37.9603399433428, Blast_Score=418, Evalue=1e-116,
Organism=Homo sapiens, GI25306287, Length=691, Percent_Identity=35.6005788712012, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI25306283, Length=391, Percent_Identity=43.7340153452685, Blast_Score=284, Evalue=2e-76,
Organism=Homo sapiens, GI157426893, Length=149, Percent_Identity=38.9261744966443, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI4503483, Length=498, Percent_Identity=24.6987951807229, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=35.8208955223881, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI310132016, Length=117, Percent_Identity=37.6068376068376, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310110807, Length=117, Percent_Identity=37.6068376068376, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310123363, Length=117, Percent_Identity=37.6068376068376, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789738, Length=695, Percent_Identity=54.8201438848921, Blast_Score=751, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=491, Percent_Identity=27.2912423625255, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1788922, Length=176, Percent_Identity=36.9318181818182, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI48994988, Length=141, Percent_Identity=38.2978723404255, Blast_Score=93, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=694, Percent_Identity=40.0576368876081, Blast_Score=505, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17556745, Length=711, Percent_Identity=29.1139240506329, Blast_Score=281, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI17506493, Length=834, Percent_Identity=23.621103117506, Blast_Score=133, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=150, Percent_Identity=40, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323098, Length=697, Percent_Identity=42.6111908177905, Blast_Score=560, Evalue=1e-160,
Organism=Saccharomyces cerevisiae, GI6322359, Length=792, Percent_Identity=30.8080808080808, Blast_Score=344, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6324707, Length=843, Percent_Identity=24.9110320284698, Blast_Score=164, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6320593, Length=843, Percent_Identity=24.9110320284698, Blast_Score=164, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=35.7615894039735, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=179, Percent_Identity=30.7262569832402, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24582462, Length=691, Percent_Identity=42.5470332850941, Blast_Score=551, Evalue=1e-157,
Organism=Drosophila melanogaster, GI221458488, Length=735, Percent_Identity=32.6530612244898, Blast_Score=354, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24585709, Length=481, Percent_Identity=25.1559251559252, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24585711, Length=481, Percent_Identity=25.1559251559252, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24585713, Length=481, Percent_Identity=25.1559251559252, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI78706572, Length=153, Percent_Identity=37.2549019607843, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28574573, Length=167, Percent_Identity=32.9341317365269, Blast_Score=86, Evalue=1e-16,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 76453; Mature: 76322

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLSLYRNIGIFAHVDAGKTTTTERILKLTGKIHRLGEVHDGASTMDFMEQEAERGITI
CCCHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEE
QSAATTCFWKGHRFNVIDTPGHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYA
EECCCEEEECCCEEEEECCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
NESEVSRLIFVNKLDRMGADFFRVVEQVKKVLGANPLVMTLPIGREDEFVGVVDVLTRQA
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCE
YVWDDSGLPENFEVKEVPADMVDQVEEYREMMIETAVEQDDELMMAYMEGEEPTVEQIKA
EEECCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
CIRKGTRDLAFFPTFCGSAFKNKGMQLVLDAVVDYLPSPTEVEPQPLTDPATGEPTGEVA
HHHCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEE
TVSVDAPLKALAFKIMDDRFGALTFVRLYSGKIKKGDTILNSATGKTERIGRMVEMHAND
EEEECCCHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCC
RNEVESAQAGDIIAIVGMKNVQTGHTLCDPKHECTLEPMIFPTPVISIAVKPKDKNGSEK
CHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCEECCCCEEEEEEECCCCCCCCH
MGIAIGKMVAEDPSFQVETDEDSGETILKGMGELHLDIKVDILKRTYGVELEVGAPQVAY
HHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCCEEEEEEEEEEHHHCCEEEECCCCCHHH
RETITKAVEDSYTHKKQSGGSGQFGKIDYRIRPGEQNSGFTFKSTVVGGNVPKEFWPAVE
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHH
KGFKSMMDTGTLAGFPVLDVEVELFDGGFHAVDSSAIAFEIAAKGAFRQSIPKAAPQLLE
HHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEEHHCCHHHHHHHHHHHHHHH
PIMKVDVFTPEDHVGDVIGDLNRRRGMIKDQEMGLTGVRVKADVPLSEMFGYIGSLRTMT
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHHHC
SGRGQFSMEFSHYAPCPNNVAEQVIAEVKERNAKK
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ADLSLYRNIGIFAHVDAGKTTTTERILKLTGKIHRLGEVHDGASTMDFMEQEAERGITI
CCHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEE
QSAATTCFWKGHRFNVIDTPGHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYA
EECCCEEEECCCEEEEECCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
NESEVSRLIFVNKLDRMGADFFRVVEQVKKVLGANPLVMTLPIGREDEFVGVVDVLTRQA
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCE
YVWDDSGLPENFEVKEVPADMVDQVEEYREMMIETAVEQDDELMMAYMEGEEPTVEQIKA
EEECCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
CIRKGTRDLAFFPTFCGSAFKNKGMQLVLDAVVDYLPSPTEVEPQPLTDPATGEPTGEVA
HHHCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEE
TVSVDAPLKALAFKIMDDRFGALTFVRLYSGKIKKGDTILNSATGKTERIGRMVEMHAND
EEEECCCHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCC
RNEVESAQAGDIIAIVGMKNVQTGHTLCDPKHECTLEPMIFPTPVISIAVKPKDKNGSEK
CHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCEECCCCEEEEEEECCCCCCCCH
MGIAIGKMVAEDPSFQVETDEDSGETILKGMGELHLDIKVDILKRTYGVELEVGAPQVAY
HHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCCEEEEEEEEEEHHHCCEEEECCCCCHHH
RETITKAVEDSYTHKKQSGGSGQFGKIDYRIRPGEQNSGFTFKSTVVGGNVPKEFWPAVE
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHH
KGFKSMMDTGTLAGFPVLDVEVELFDGGFHAVDSSAIAFEIAAKGAFRQSIPKAAPQLLE
HHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEEHHCCHHHHHHHHHHHHHHH
PIMKVDVFTPEDHVGDVIGDLNRRRGMIKDQEMGLTGVRVKADVPLSEMFGYIGSLRTMT
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHHHC
SGRGQFSMEFSHYAPCPNNVAEQVIAEVKERNAKK
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]