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The map label for this gene is radA [H]

Identifier: 147673564

GI number: 147673564

Start: 2050984

End: 2052363

Strand: Reverse

Name: radA [H]

Synonym: VC0395_A1922

Alternate gene names: 147673564

Gene position: 2052363-2050984 (Counterclockwise)

Preceding gene: 147674858

Following gene: 147673153

Centisome position: 67.87

GC content: 49.57

Gene sequence:

>1380_bases
TTGATTGTGAAAGCTAAGCGCGCCTATGTATGTAATGAATGTGGAGCGGATTTTCCGCGTTGGCAAGGACAGTGTAATGC
CTGTGGTTCGTGGAATTCGATTTCAGAAGTTCGTTTAGCGGCTTCACCGCAAGTGGCGCGCAATGAAAGGTTGGTCGGTT
ATGCGGGAGCTCTTGAAGCGAAAGTACAAACTTTGGCTGATATTGATTTACAAGAAGTGCCTCGATTCACCAGTGGCTTT
AAAGAACTAGATCGCGTACTTGGCGGCGGAATTGTGCCGGGTGCGGCGATTTTGATTGGTGGTAATCCGGGCGCGGGTAA
ATCGACCTTACTACTGCAAACCATGTGTTTACTTTCCGGACAGATGCCGACGTTGTACGTGACGGGCGAAGAATCGTTGC
AGCAGGTCGCGATGCGTGCTTCACGGCTTGGTTTGCCGAAACAGCATCTAAAAATGCTCTCAGAAACCAATGTGGATCGG
ATTTGCCAGATTGCTGAGCAAGAAAAGCCGCGCATTATGGTCATCGATTCGATTCAAGTGATGCATGTGGCGGATGTGCA
ATCGTCACCGGGGAGTGTCGCGCAAGTGCGTGAGTCGGCCACGGCGCTTACGCGTTATGCTAAACAAAATAATGTGGCGG
TATTTATTGTGGGCCACGTAACCAAAGATGGTACGTTGGCGGGACCTAAAGTTCTTGAACACATCATCGATTGCTCGATT
CTGCTTGATGGCGATACCGACAGCCGTTTTCGTACACTCCGTAGTCATAAAAACCGTTTTGGTGCGGTCAATGAACTCGG
TGTGTTTGCGATGACTGGACAAGGGATGCGTGAGGTGAGTAATCCATCGGCGATTTTCCTTTCTCGTGGAGAAGAAGCCA
CATCTGGTAGCTCAGTGATGGTGGTGTGGGAAGGAACTCGCCCACTCTTAGTTGAGATTCAAGCGTTGGTCGATTACTCA
CAACTCGCTAACCCACGACGCGTTGCGGTTGGCTTAGAACAAAACCGTCTCTCTTTATTACTGGCCGTGCTGCATAAACA
CGGTGGTTTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTAGTCGGTGGCGTGAAAGTGACGGAGACCAGTGCAGATT
TAGCGCTACTCATGGCGTTATTGTCGAGTTTTCGTGATCGTCCGCTGCCAAAAGATGTCGTGGTTTTTGGTGAAGTCGGA
TTGGCTGGAGAAATTCGTCCCGTACCGAGTGGTCAAGAGCGCCTTAACGAAGCCTTCAAACATGGTTTTAAGAAAGCGAT
TGTGCCGATTGCCAATATGCCGAAAGGTGGTATTGAAGGTATGCAGATCCACGGTGTGAAAAAGCTTTCTGATGCGATCG
CGGCTTTTGATGAGCTGTAA

Upstream 100 bases:

>100_bases
TGATGCATCGGCATCAACATACTAATCTTTAGCGATATGTTGCAGCCGTTCCACTTTTTCTTAAAGTATTACGCCTGATA
AGTGAGAGCAAGTTCGAGGA

Downstream 100 bases:

>100_bases
TGGGTAGTTGGTTTTGAACACGAATGACGCCAAAAAGTGCAGCGTAAAAATATCATGGTTGCGCCTCGCGAGCCGTTTGA
GGATCACCTATAGTTTCTCA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MIVKAKRAYVCNECGADFPRWQGQCNACGSWNSISEVRLAASPQVARNERLVGYAGALEAKVQTLADIDLQEVPRFTSGF
KELDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCLLSGQMPTLYVTGEESLQQVAMRASRLGLPKQHLKMLSETNVDR
ICQIAEQEKPRIMVIDSIQVMHVADVQSSPGSVAQVRESATALTRYAKQNNVAVFIVGHVTKDGTLAGPKVLEHIIDCSI
LLDGDTDSRFRTLRSHKNRFGAVNELGVFAMTGQGMREVSNPSAIFLSRGEEATSGSSVMVVWEGTRPLLVEIQALVDYS
QLANPRRVAVGLEQNRLSLLLAVLHKHGGLQMADQDVFVNVVGGVKVTETSADLALLMALLSSFRDRPLPKDVVVFGEVG
LAGEIRPVPSGQERLNEAFKHGFKKAIVPIANMPKGGIEGMQIHGVKKLSDAIAAFDEL

Sequences:

>Translated_459_residues
MIVKAKRAYVCNECGADFPRWQGQCNACGSWNSISEVRLAASPQVARNERLVGYAGALEAKVQTLADIDLQEVPRFTSGF
KELDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCLLSGQMPTLYVTGEESLQQVAMRASRLGLPKQHLKMLSETNVDR
ICQIAEQEKPRIMVIDSIQVMHVADVQSSPGSVAQVRESATALTRYAKQNNVAVFIVGHVTKDGTLAGPKVLEHIIDCSI
LLDGDTDSRFRTLRSHKNRFGAVNELGVFAMTGQGMREVSNPSAIFLSRGEEATSGSSVMVVWEGTRPLLVEIQALVDYS
QLANPRRVAVGLEQNRLSLLLAVLHKHGGLQMADQDVFVNVVGGVKVTETSADLALLMALLSSFRDRPLPKDVVVFGEVG
LAGEIRPVPSGQERLNEAFKHGFKKAIVPIANMPKGGIEGMQIHGVKKLSDAIAAFDEL
>Mature_459_residues
MIVKAKRAYVCNECGADFPRWQGQCNACGSWNSISEVRLAASPQVARNERLVGYAGALEAKVQTLADIDLQEVPRFTSGF
KELDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCLLSGQMPTLYVTGEESLQQVAMRASRLGLPKQHLKMLSETNVDR
ICQIAEQEKPRIMVIDSIQVMHVADVQSSPGSVAQVRESATALTRYAKQNNVAVFIVGHVTKDGTLAGPKVLEHIIDCSI
LLDGDTDSRFRTLRSHKNRFGAVNELGVFAMTGQGMREVSNPSAIFLSRGEEATSGSSVMVVWEGTRPLLVEIQALVDYS
QLANPRRVAVGLEQNRLSLLLAVLHKHGGLQMADQDVFVNVVGGVKVTETSADLALLMALLSSFRDRPLPKDVVVFGEVG
LAGEIRPVPSGQERLNEAFKHGFKKAIVPIANMPKGGIEGMQIHGVKKLSDAIAAFDEL

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=455, Percent_Identity=80.8791208791209, Blast_Score=772, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49412; Mature: 49412

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVKAKRAYVCNECGADFPRWQGQCNACGSWNSISEVRLAASPQVARNERLVGYAGALEA
CEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEECCCCCHHCCCEEEEEHHHHHH
KVQTLADIDLQEVPRFTSGFKELDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCLLSG
HHHHHHCCCHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCC
QMPTLYVTGEESLQQVAMRASRLGLPKQHLKMLSETNVDRICQIAEQEKPRIMVIDSIQV
CCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECEEE
MHVADVQSSPGSVAQVRESATALTRYAKQNNVAVFIVGHVTKDGTLAGPKVLEHIIDCSI
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHCEEE
LLDGDTDSRFRTLRSHKNRFGAVNELGVFAMTGQGMREVSNPSAIFLSRGEEATSGSSVM
EEECCCCHHHHHHHHHHHHCCCHHHCCEEEECCCCHHHCCCCCEEEEECCCCCCCCCEEE
VVWEGTRPLLVEIQALVDYSQLANPRRVAVGLEQNRLSLLLAVLHKHGGLQMADQDVFVN
EEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEECCCEEEE
VVGGVKVTETSADLALLMALLSSFRDRPLPKDVVVFGEVGLAGEIRPVPSGQERLNEAFK
EECCEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
HGFKKAIVPIANMPKGGIEGMQIHGVKKLSDAIAAFDEL
HHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIVKAKRAYVCNECGADFPRWQGQCNACGSWNSISEVRLAASPQVARNERLVGYAGALEA
CEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEECCCCCHHCCCEEEEEHHHHHH
KVQTLADIDLQEVPRFTSGFKELDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCLLSG
HHHHHHCCCHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCC
QMPTLYVTGEESLQQVAMRASRLGLPKQHLKMLSETNVDRICQIAEQEKPRIMVIDSIQV
CCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECEEE
MHVADVQSSPGSVAQVRESATALTRYAKQNNVAVFIVGHVTKDGTLAGPKVLEHIIDCSI
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHCEEE
LLDGDTDSRFRTLRSHKNRFGAVNELGVFAMTGQGMREVSNPSAIFLSRGEEATSGSSVM
EEECCCCHHHHHHHHHHHHCCCHHHCCEEEECCCCHHHCCCCCEEEEECCCCCCCCCEEE
VVWEGTRPLLVEIQALVDYSQLANPRRVAVGLEQNRLSLLLAVLHKHGGLQMADQDVFVN
EEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEECCCEEEE
VVGGVKVTETSADLALLMALLSSFRDRPLPKDVVVFGEVGLAGEIRPVPSGQERLNEAFK
EECCEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
HGFKKAIVPIANMPKGGIEGMQIHGVKKLSDAIAAFDEL
HHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]