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The map label for this gene is rfbB [H]

Identifier: 146343782

GI number: 146343782

Start: 7332449

End: 7333522

Strand: Direct

Name: rfbB [H]

Synonym: BRADO7029

Alternate gene names: 146343782

Gene position: 7332449-7333522 (Clockwise)

Preceding gene: 146343781

Following gene: 146343797

Centisome position: 98.34

GC content: 66.95

Gene sequence:

>1074_bases
ATGCGTTTCAAGGGCTCGACCATCTTCGTCACCGGCGGCGCCGGCTTCATCGGCTCGGCCGTGGTGCGGCACCTCTTGCG
CGACACCCATGCCCGCGTGGTCAACATCGACAAGCTGACCTACGCCGCCAATCTCGATTCGCTGCCCGGCGCGGCCGGCA
ATCCGCATTACGCCTTCGAGCAGGCCGATATCTGCGACGCGGCCGCCTTGCGCAAGCTGTTCGACAAGTACCAGCCCGAC
GCGGTGATGAACCTCGCCGCCGAGAGCCATGTCGACCGCTCGATCGACGGCCCCGGCGAGTTCATCCAGACCAACATCGT
CGGCACCTTCACGCTGCTGCAGGAGGCGCTGCGCCACTTCCGCAGCCTGTCTCCGGAGAAGCGCGCCGGCTTCCGCTTCC
TGCACATCTCGACCGACGAGGTGTTCGGCACGCTCGGCGACACCGGCCTGTTCACCGAGACCACGGCCTACGCGCCCAAC
TCGCCCTATTCGGCGAGCAAGGCCTCGTCCGACCATCTGGTGCGGGCCTGGCGCGAGACCTACGAGCTGCCGACCATGAT
CACCAACTGCTCGAACAATTACGGGCCGTATCACTTCCCCGAGAAGCTGATCCCGCACATGATCATCAAGGGCCTCGGCT
TCGAGACGCTGCCGGTCTATGGCGACGGCCAGAACATCCGCGACTGGCTGTTCGTCGAGGACCACGCGAGGGCGTTGACC
CTGGTGCTGGAGCGCGGCCAGGTCGGCGAGACCTACAATGTCGGCGGCCGCAACGAGCGCACCAATCTGCACGTGGTCGA
GACCATCTGCGACCTGCTCGACGCGGTCGCGCCGGGGCCGCAAGGCGGCCGCCGCCAGCTGATCAGCTTCGTCACCGACC
GCCCCGGCCACGACCGCCGCTACGCCATCGACGCCTCCAAGCTGGAGCGCGAGCTCGGCTGGCGCGCCGAGGAGACTTTC
GAGACCGGCATCGCCAAGACCGTCCGCTGGTATGTCCAGGAGAAACCGTGGTGGCAGGCGATCCTGCAGCGCGGCTATCA
GACCGAGCGGCTCGGCCGCAAGGCAGCGCATTGA

Upstream 100 bases:

>100_bases
GCAGTTCGACAAGGTCGCAGCCCGAACCGCCAAGAGCAGCTACGGCGAATATCTGCAGTCCGTCTCCCGCTCCTTCCACC
GCTGACGCCTGGAGGCATCG

Downstream 100 bases:

>100_bases
CCCTCCGATGGTCAGCGCCGGCCTCGACCTCCGGGACGAGCCGCCGCGGCCTCGCGGCGCGATGAGCGCCCGAGCTGTGC
TGAAATCCGGACCCTCGCAA

Product: dTDP-D-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MRFKGSTIFVTGGAGFIGSAVVRHLLRDTHARVVNIDKLTYAANLDSLPGAAGNPHYAFEQADICDAAALRKLFDKYQPD
AVMNLAAESHVDRSIDGPGEFIQTNIVGTFTLLQEALRHFRSLSPEKRAGFRFLHISTDEVFGTLGDTGLFTETTAYAPN
SPYSASKASSDHLVRAWRETYELPTMITNCSNNYGPYHFPEKLIPHMIIKGLGFETLPVYGDGQNIRDWLFVEDHARALT
LVLERGQVGETYNVGGRNERTNLHVVETICDLLDAVAPGPQGGRRQLISFVTDRPGHDRRYAIDASKLERELGWRAEETF
ETGIAKTVRWYVQEKPWWQAILQRGYQTERLGRKAAH

Sequences:

>Translated_357_residues
MRFKGSTIFVTGGAGFIGSAVVRHLLRDTHARVVNIDKLTYAANLDSLPGAAGNPHYAFEQADICDAAALRKLFDKYQPD
AVMNLAAESHVDRSIDGPGEFIQTNIVGTFTLLQEALRHFRSLSPEKRAGFRFLHISTDEVFGTLGDTGLFTETTAYAPN
SPYSASKASSDHLVRAWRETYELPTMITNCSNNYGPYHFPEKLIPHMIIKGLGFETLPVYGDGQNIRDWLFVEDHARALT
LVLERGQVGETYNVGGRNERTNLHVVETICDLLDAVAPGPQGGRRQLISFVTDRPGHDRRYAIDASKLERELGWRAEETF
ETGIAKTVRWYVQEKPWWQAILQRGYQTERLGRKAAH
>Mature_357_residues
MRFKGSTIFVTGGAGFIGSAVVRHLLRDTHARVVNIDKLTYAANLDSLPGAAGNPHYAFEQADICDAAALRKLFDKYQPD
AVMNLAAESHVDRSIDGPGEFIQTNIVGTFTLLQEALRHFRSLSPEKRAGFRFLHISTDEVFGTLGDTGLFTETTAYAPN
SPYSASKASSDHLVRAWRETYELPTMITNCSNNYGPYHFPEKLIPHMIIKGLGFETLPVYGDGQNIRDWLFVEDHARALT
LVLERGQVGETYNVGGRNERTNLHVVETICDLLDAVAPGPQGGRRQLISFVTDRPGHDRRYAIDASKLERELGWRAEETF
ETGIAKTVRWYVQEKPWWQAILQRGYQTERLGRKAAH

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=335, Percent_Identity=33.7313432835821, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI42516563, Length=336, Percent_Identity=24.702380952381, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI56237023, Length=356, Percent_Identity=27.5280898876405, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI56118217, Length=356, Percent_Identity=27.5280898876405, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI189083684, Length=356, Percent_Identity=27.5280898876405, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4504031, Length=341, Percent_Identity=24.633431085044, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=63.8176638176638, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI1788353, Length=350, Percent_Identity=60.5714285714286, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1786974, Length=348, Percent_Identity=25.2873563218391, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788366, Length=304, Percent_Identity=25.6578947368421, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=336, Percent_Identity=34.5238095238095, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI115532424, Length=346, Percent_Identity=30.635838150289, Blast_Score=157, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI71982035, Length=355, Percent_Identity=27.3239436619718, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71982038, Length=357, Percent_Identity=26.6106442577031, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=360, Percent_Identity=22.2222222222222, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=342, Percent_Identity=25.7309941520468, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=342, Percent_Identity=25.7309941520468, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=342, Percent_Identity=25.7309941520468, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=342, Percent_Identity=25.7309941520468, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=342, Percent_Identity=25.7309941520468, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=342, Percent_Identity=23.9766081871345, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=369, Percent_Identity=24.9322493224932, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19923002, Length=357, Percent_Identity=25.2100840336134, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356223, Length=336, Percent_Identity=23.8095238095238, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24158427, Length=333, Percent_Identity=26.4264264264264, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40010; Mature: 40010

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFKGSTIFVTGGAGFIGSAVVRHLLRDTHARVVNIDKLTYAANLDSLPGAAGNPHYAFE
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEEHHHEEECCCCCCCCCCCCCCCCCC
QADICDAAALRKLFDKYQPDAVMNLAAESHVDRSIDGPGEFIQTNIVGTFTLLQEALRHF
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RSLSPEKRAGFRFLHISTDEVFGTLGDTGLFTETTAYAPNSPYSASKASSDHLVRAWRET
HCCCCCHHCCEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
YELPTMITNCSNNYGPYHFPEKLIPHMIIKGLGFETLPVYGDGQNIRDWLFVEDHARALT
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEECHHHHEEH
LVLERGQVGETYNVGGRNERTNLHVVETICDLLDAVAPGPQGGRRQLISFVTDRPGHDRR
HHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCE
YAIDASKLERELGWRAEETFETGIAKTVRWYVQEKPWWQAILQRGYQTERLGRKAAH
EEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure
MRFKGSTIFVTGGAGFIGSAVVRHLLRDTHARVVNIDKLTYAANLDSLPGAAGNPHYAFE
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEEHHHEEECCCCCCCCCCCCCCCCCC
QADICDAAALRKLFDKYQPDAVMNLAAESHVDRSIDGPGEFIQTNIVGTFTLLQEALRHF
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RSLSPEKRAGFRFLHISTDEVFGTLGDTGLFTETTAYAPNSPYSASKASSDHLVRAWRET
HCCCCCHHCCEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
YELPTMITNCSNNYGPYHFPEKLIPHMIIKGLGFETLPVYGDGQNIRDWLFVEDHARALT
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEECHHHHEEH
LVLERGQVGETYNVGGRNERTNLHVVETICDLLDAVAPGPQGGRRQLISFVTDRPGHDRR
HHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCE
YAIDASKLERELGWRAEETFETGIAKTVRWYVQEKPWWQAILQRGYQTERLGRKAAH
EEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8022265; 10710307 [H]