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The map label for this gene is rfbA [H]

Identifier: 146343781

GI number: 146343781

Start: 7331558

End: 7332433

Strand: Direct

Name: rfbA [H]

Synonym: BRADO7028

Alternate gene names: 146343781

Gene position: 7331558-7332433 (Clockwise)

Preceding gene: 146343777

Following gene: 146343782

Centisome position: 98.32

GC content: 63.36

Gene sequence:

>876_bases
ATGAAGGGCATTATCCTCGCCGGAGGCACGGGCTCGCGCCTGTATCCGGTGACGACGGTTGTTTCCAAGCAATTGCTGCC
GGTGTTCGACAAGCCGATGATCTATTATCCGCTGTCGACGCTGATGCTGGCCGGTATTCGCGACATTCTCATCATTTCGA
CGCCGCAGGACAAGCCGCTGTTCCAGCGGCTGCTCGGCGACGGCAGTGAGATCGGGCTGCGATTTCAATATGCGACCCAG
GAGACACCGCGCGGACTCGCGGACGCGTTCATCGTCGGCCGCGAGTTCATCGGCAATGATTCCGTCGCGCTGATCCTCGG
CGACAACATCTTCTACGGCCACGGCCTGCCGAGCATGCTGTCGAGCGCCTCGTTCCGCAAGAAAGGCGCGACCGTGTTCG
GCTATGTCGTCAACGAGCCGCAGGCCTATGGCGTGGTCGAGCTCGACGGCACGGGACGTGCGCTCTCGATCGAGGAGAAG
CCGAAGCAGCCGAAGTCCAACGTCGCGGTCACCGGCCTCTATTTCTACGACAACGACGTCGTCAGCATCGCCGCGGACGT
CAAGCCGTCGGCGCGTGGCGAGATCGAGATCACCGACGTCAACAACGCCTATCTGAAGCGCGGTGACCTCTATGTCGAGG
TGCTCGGCCGCGGCTTCGCCTGGCTCGACACCGGCACGCATGCCTCCCTCGTCGAGGCCAGCCATTTCGTGCAGATCCTC
GAGCAGCGCCAGGGCCTGCGCATCGCCTGCCCCGAGGAGATCGCGCTGCGCCAGGGCTACATCTCGCTGGAGCAGTTCGA
CAAGGTCGCAGCCCGAACCGCCAAGAGCAGCTACGGCGAATATCTGCAGTCCGTCTCCCGCTCCTTCCACCGCTGA

Upstream 100 bases:

>100_bases
CTAAATGGATTGCGCTTCTACAATTTACTGTATCCCTTTATCCAACTTGTGCAACGCACGCTATCTGTTAACATAACAAT
TATTCGCTGAGTGAGGTCCC

Downstream 100 bases:

>100_bases
CGCCTGGAGGCATCGATGCGTTTCAAGGGCTCGACCATCTTCGTCACCGGCGGCGCCGGCTTCATCGGCTCGGCCGTGGT
GCGGCACCTCTTGCGCGACA

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 1; dTDP-glucose pyrophosphorylase 1; dTDP-glucose synthase 1 [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKGIILAGGTGSRLYPVTTVVSKQLLPVFDKPMIYYPLSTLMLAGIRDILIISTPQDKPLFQRLLGDGSEIGLRFQYATQ
ETPRGLADAFIVGREFIGNDSVALILGDNIFYGHGLPSMLSSASFRKKGATVFGYVVNEPQAYGVVELDGTGRALSIEEK
PKQPKSNVAVTGLYFYDNDVVSIAADVKPSARGEIEITDVNNAYLKRGDLYVEVLGRGFAWLDTGTHASLVEASHFVQIL
EQRQGLRIACPEEIALRQGYISLEQFDKVAARTAKSSYGEYLQSVSRSFHR

Sequences:

>Translated_291_residues
MKGIILAGGTGSRLYPVTTVVSKQLLPVFDKPMIYYPLSTLMLAGIRDILIISTPQDKPLFQRLLGDGSEIGLRFQYATQ
ETPRGLADAFIVGREFIGNDSVALILGDNIFYGHGLPSMLSSASFRKKGATVFGYVVNEPQAYGVVELDGTGRALSIEEK
PKQPKSNVAVTGLYFYDNDVVSIAADVKPSARGEIEITDVNNAYLKRGDLYVEVLGRGFAWLDTGTHASLVEASHFVQIL
EQRQGLRIACPEEIALRQGYISLEQFDKVAARTAKSSYGEYLQSVSRSFHR
>Mature_291_residues
MKGIILAGGTGSRLYPVTTVVSKQLLPVFDKPMIYYPLSTLMLAGIRDILIISTPQDKPLFQRLLGDGSEIGLRFQYATQ
ETPRGLADAFIVGREFIGNDSVALILGDNIFYGHGLPSMLSSASFRKKGATVFGYVVNEPQAYGVVELDGTGRALSIEEK
PKQPKSNVAVTGLYFYDNDVVSIAADVKPSARGEIEITDVNNAYLKRGDLYVEVLGRGFAWLDTGTHASLVEASHFVQIL
EQRQGLRIACPEEIALRQGYISLEQFDKVAARTAKSSYGEYLQSVSRSFHR

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761619, Length=252, Percent_Identity=26.5873015873016, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI11761621, Length=252, Percent_Identity=26.5873015873016, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788351, Length=286, Percent_Identity=65.7342657342657, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1790224, Length=291, Percent_Identity=61.8556701030928, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1787488, Length=242, Percent_Identity=26.4462809917355, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788355, Length=237, Percent_Identity=23.6286919831224, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133931050, Length=226, Percent_Identity=26.9911504424779, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320148, Length=253, Percent_Identity=25.6916996047431, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355443, Length=249, Percent_Identity=24.8995983935743, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24644084, Length=249, Percent_Identity=24.8995983935743, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 31921; Mature: 31921

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGTGSRLYPVTTVVSKQLLPVFDKPMIYYPLSTLMLAGIRDILIISTPQDKPL
CCCEEEECCCCCCEEEHHHHHHHHHHHHHCCCEEEECHHHHHHHCCCEEEEEECCCCCHH
FQRLLGDGSEIGLRFQYATQETPRGLADAFIVGREFIGNDSVALILGDNIFYGHGLPSML
HHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCHHHH
SSASFRKKGATVFGYVVNEPQAYGVVELDGTGRALSIEEKPKQPKSNVAVTGLYFYDNDV
HHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCCE
VSIAADVKPSARGEIEITDVNNAYLKRGDLYVEVLGRGFAWLDTGTHASLVEASHFVQIL
EEEEECCCCCCCCCEEEEECCHHHHHCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHH
EQRQGLRIACPEEIALRQGYISLEQFDKVAARTAKSSYGEYLQSVSRSFHR
HHHCCCEEECCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGIILAGGTGSRLYPVTTVVSKQLLPVFDKPMIYYPLSTLMLAGIRDILIISTPQDKPL
CCCEEEECCCCCCEEEHHHHHHHHHHHHHCCCEEEECHHHHHHHCCCEEEEEECCCCCHH
FQRLLGDGSEIGLRFQYATQETPRGLADAFIVGREFIGNDSVALILGDNIFYGHGLPSML
HHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCHHHH
SSASFRKKGATVFGYVVNEPQAYGVVELDGTGRALSIEEKPKQPKSNVAVTGLYFYDNDV
HHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCCE
VSIAADVKPSARGEIEITDVNNAYLKRGDLYVEVLGRGFAWLDTGTHASLVEASHFVQIL
EEEEECCCCCCCCCEEEEECCHHHHHCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHH
EQRQGLRIACPEEIALRQGYISLEQFDKVAARTAKSSYGEYLQSVSRSFHR
HHHCCCEEECCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503; 7517390 [H]