Click here to switch to the map view.

The map label for this gene is rfbD [H]

Identifier: 146343779

GI number: 146343779

Start: 7329984

End: 7330892

Strand: Reverse

Name: rfbD [H]

Synonym: BRADO7026

Alternate gene names: 146343779

Gene position: 7330892-7329984 (Counterclockwise)

Preceding gene: 146343780

Following gene: 146343778

Centisome position: 98.31

GC content: 72.72

Gene sequence:

>909_bases
ATGCGCATTCTGATCACGGGCACCAGCGGACAGGTCGGCGGCGCGCTGCGCAGGCTGCTGCCGGCCGGTGGAACCGATGA
GCTGATCTGCCCGCCGCGCGCGGCGTTCGATCTGACGCAGCCCGCCAGCCTCGCGGGCGCGCTGGACCGGCTCGCGCCCG
ACCTCATTCTCAATCCGGCGGCCTATACCGCCGTCGATCGCGCCGAGGACGAGGCCGAGCTCGCGATGACCGTCAACGGC
CAGGCGCCGGGCGTGATCGCGCAATGGGCGGCGCCGCGGCGCGTGCCGCTGCTGCATGTCTCGACCGACTACGTGTTCGA
CGGCGCGGGCGAGCACCCCTGGCGCGAGAGCGACGCCGTCGGTCCGCTCTCGGCCTATGGCCGCAGCAAGCTCGCCGGCG
AGGACGCGGTGCGCAGCGCCGGCGGCCCGCATCTGATCGTGCGCACGGCCTGGGTGTTCGCGTCCGCAGGCGCGAATTTC
ATGCGCACGATCGTCCGCCTGGCGCGGGAGCGCGAGGCCCTGCGTGTCGTCGCCGACCAGAAGGGGACGCCGACCTCGGC
GCGGACCATCGCGGAGGTGATCGCCGCGATCCTCGCGCAAGGTGCCGGCGACCTGCCGGGCGCATTCGCACGGGCCGAGG
GCCTCGTGCATCTGACCAATTCCGGCGCCACCAGCTGGCACGGCTTCGCCAGCGCCATCGTCGACGGTCTGCGACAGCGT
AACGTGCCGGTGAAGGCTCGGGCGGTCGAGGCGATCACCACGGCGGATTTTCCGACCAAGGCGCGGCGGCCGGCCAATTC
TCAGCTCGATCTGTCGCGCCTCCGTGGCGCCTACGGCCTCGTGCCGCCGTCCTGGCAGCAGGCCCTGTCCCGCGAGCTCG
ACGAGCTCGTCGCGGCCGAGCAGCGCTAG

Upstream 100 bases:

>100_bases
GCATCGACTGGGGCGTGCCAGAGCCGAAGCTGTCGCCCAAGGACGCCGAGGCGCCGCTGCTGGCGGAGGCCCGGAATCTT
CCCGTGTACGGGCAGATCTG

Downstream 100 bases:

>100_bases
GACCTGTTCGCCACAGTCGCCCGCGCGCTGACGGGCCGTTCGCCCAAGCCTGTTGTCTTTTCCCAGGGGATCGGCAAAGA
CCACGGCTGCCACCCGTCGA

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MRILITGTSGQVGGALRRLLPAGGTDELICPPRAAFDLTQPASLAGALDRLAPDLILNPAAYTAVDRAEDEAELAMTVNG
QAPGVIAQWAAPRRVPLLHVSTDYVFDGAGEHPWRESDAVGPLSAYGRSKLAGEDAVRSAGGPHLIVRTAWVFASAGANF
MRTIVRLAREREALRVVADQKGTPTSARTIAEVIAAILAQGAGDLPGAFARAEGLVHLTNSGATSWHGFASAIVDGLRQR
NVPVKARAVEAITTADFPTKARRPANSQLDLSRLRGAYGLVPPSWQQALSRELDELVAAEQR

Sequences:

>Translated_302_residues
MRILITGTSGQVGGALRRLLPAGGTDELICPPRAAFDLTQPASLAGALDRLAPDLILNPAAYTAVDRAEDEAELAMTVNG
QAPGVIAQWAAPRRVPLLHVSTDYVFDGAGEHPWRESDAVGPLSAYGRSKLAGEDAVRSAGGPHLIVRTAWVFASAGANF
MRTIVRLAREREALRVVADQKGTPTSARTIAEVIAAILAQGAGDLPGAFARAEGLVHLTNSGATSWHGFASAIVDGLRQR
NVPVKARAVEAITTADFPTKARRPANSQLDLSRLRGAYGLVPPSWQQALSRELDELVAAEQR
>Mature_302_residues
MRILITGTSGQVGGALRRLLPAGGTDELICPPRAAFDLTQPASLAGALDRLAPDLILNPAAYTAVDRAEDEAELAMTVNG
QAPGVIAQWAAPRRVPLLHVSTDYVFDGAGEHPWRESDAVGPLSAYGRSKLAGEDAVRSAGGPHLIVRTAWVFASAGANF
MRTIVRLAREREALRVVADQKGTPTSARTIAEVIAAILAQGAGDLPGAFARAEGLVHLTNSGATSWHGFASAIVDGLRQR
NVPVKARAVEAITTADFPTKARRPANSQLDLSRLRGAYGLVPPSWQQALSRELDELVAAEQR

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=286, Percent_Identity=26.2237762237762, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI11034825, Length=286, Percent_Identity=26.2237762237762, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788352, Length=299, Percent_Identity=40.133779264214, Blast_Score=221, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 31884; Mature: 31884

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILITGTSGQVGGALRRLLPAGGTDELICPPRAAFDLTQPASLAGALDRLAPDLILNPA
CEEEEECCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHCHHHHCCCH
AYTAVDRAEDEAELAMTVNGQAPGVIAQWAAPRRVPLLHVSTDYVFDGAGEHPWRESDAV
HHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCCCCEEEEEECCEEECCCCCCCCCCCCCC
GPLSAYGRSKLAGEDAVRSAGGPHLIVRTAWVFASAGANFMRTIVRLAREREALRVVADQ
CCHHHHCCHHCCCHHHHHHCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
KGTPTSARTIAEVIAAILAQGAGDLPGAFARAEGLVHLTNSGATSWHGFASAIVDGLRQR
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHC
NVPVKARAVEAITTADFPTKARRPANSQLDLSRLRGAYGLVPPSWQQALSRELDELVAAE
CCCCHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
QR
CC
>Mature Secondary Structure
MRILITGTSGQVGGALRRLLPAGGTDELICPPRAAFDLTQPASLAGALDRLAPDLILNPA
CEEEEECCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHCHHHHCCCH
AYTAVDRAEDEAELAMTVNGQAPGVIAQWAAPRRVPLLHVSTDYVFDGAGEHPWRESDAV
HHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCCCCEEEEEECCEEECCCCCCCCCCCCCC
GPLSAYGRSKLAGEDAVRSAGGPHLIVRTAWVFASAGANFMRTIVRLAREREALRVVADQ
CCHHHHCCHHCCCHHHHHHCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
KGTPTSARTIAEVIAAILAQGAGDLPGAFARAEGLVHLTNSGATSWHGFASAIVDGLRQR
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHC
NVPVKARAVEAITTADFPTKARRPANSQLDLSRLRGAYGLVPPSWQQALSRELDELVAAE
CCCCHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
QR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]