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The map label for this gene is fadE25 [H]

Identifier: 146343603

GI number: 146343603

Start: 7102692

End: 7103891

Strand: Reverse

Name: fadE25 [H]

Synonym: BRADO6841

Alternate gene names: 146343603

Gene position: 7103891-7102692 (Counterclockwise)

Preceding gene: 146343605

Following gene: 146343602

Centisome position: 95.27

GC content: 66.33

Gene sequence:

>1200_bases
ATGGTCGATTCAAAACTGGCCGATCTCGACACATTCCGCCGCGAAACGCGTGCCTGGCTCGAGGCGAATTGCCCGCCCGA
AATGCGCCGGCCGATGACCTCCGACGAGGACACTTTCTGGGGCGGCCGCAACGCCAAGTTCTCCTCGGAGCCGCAGCGCA
TCTGGTTCGAGCGCATGCGCGACAAGGGCTGGACGGTGCCGCACTGGCCGAAAGAATACGGGGGCGGCGGTCTCGACGGC
GAGGAGGCCAAGATCGTGCGTCAGGAGATGGCCGCGATCGGCGCGCGCGCCCCGATCACCTCGTTCGGCATCTCGATGCT
CGGGCCGGCGCTCTTGAAATACGGCACCGACGCGCAGAAGCGCGAACACCTGCCGAAGATCACCGCCGGTCTCATCCGCT
GGTGCCAGGGCTATTCCGAGCCGAACGCCGGCTCCGATCTCGCCTCGCTGCAGACCCGCGCCGAGAGCGATGGCGACGAC
TACATCATCAACGGCCAGAAGATCTGGACGTCCTACGCCAACTACGCCGACTGGATCTTCTGCCTGGTGCGCACCGATCC
CGCGGCGAAGAAGCATGACGGCATCAGTTTCATCCTGTTCGACATGACGTCGAAGGGGGTCTCGACCAAGCCGATCCTGC
TGATCTCCGGCCGCTCGCCGTTCTGCGAGACCTTCTTCGACAATGTCCGGGTGCCCAAGGCCAATGTGCTCGGCACCGTC
AATCGCGGCTGGGACGTCGCGAAATATCTGCTGCAGCACGAGCGCGCAATGATCTCCGGCATGGGCGAGCGCGGCGGAAG
CCGTCCGCTCGGCCAGGTGGCGGCCGACTCCGTCGGCAGCGATGCGCAAGGCAAGCTCGACGATGCGCTGCTGCGCGCAC
AGATCGCGACCTTCGACGTCGACGAGGCGGCGCTCGCGGCCGCCGCCGAGCGCATGGTCGATCTCGCCAAGGCGGGGCAG
GGCCATCCGGCGTTCTCCTCAGCGATGAAATATTACGGCACCGAGCTCAACAAGCGCCGCCATGAGATCCTGATGTCGGC
GGGCGGCATCGACGCGCTGGAATGGGAAAGCGAGCGCTCTGGCGAGGGCGCCCGCCCGCGCGCCTGGCTGCGCACGAAAG
CGAACTCCATCGAAGGCGGCACCTCCGAGGTCATGCTCGGCATCGTCGCCAAGCGCATCCTCGATCTGCCGGGGGCGTAG

Upstream 100 bases:

>100_bases
GAGCGCAGCCCGATGCGGCGACATCGATATTGAATTTCAGTAAGGGCTGAGGTTAGGTCGTTCCAACGACAGCAGACATG
GAAACGCGCGAGGATCAGAC

Downstream 100 bases:

>100_bases
TTCACCCCACACTCCGTCATGCCCGGGCTCGACCCGGGCATCCACGTCCATCCGCACGAGGAGAGACGTGGATGGCCGGG
TCAAGCCCGGCCATGACGAG

Product: putative acyl-CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MVDSKLADLDTFRRETRAWLEANCPPEMRRPMTSDEDTFWGGRNAKFSSEPQRIWFERMRDKGWTVPHWPKEYGGGGLDG
EEAKIVRQEMAAIGARAPITSFGISMLGPALLKYGTDAQKREHLPKITAGLIRWCQGYSEPNAGSDLASLQTRAESDGDD
YIINGQKIWTSYANYADWIFCLVRTDPAAKKHDGISFILFDMTSKGVSTKPILLISGRSPFCETFFDNVRVPKANVLGTV
NRGWDVAKYLLQHERAMISGMGERGGSRPLGQVAADSVGSDAQGKLDDALLRAQIATFDVDEAALAAAAERMVDLAKAGQ
GHPAFSSAMKYYGTELNKRRHEILMSAGGIDALEWESERSGEGARPRAWLRTKANSIEGGTSEVMLGIVAKRILDLPGA

Sequences:

>Translated_399_residues
MVDSKLADLDTFRRETRAWLEANCPPEMRRPMTSDEDTFWGGRNAKFSSEPQRIWFERMRDKGWTVPHWPKEYGGGGLDG
EEAKIVRQEMAAIGARAPITSFGISMLGPALLKYGTDAQKREHLPKITAGLIRWCQGYSEPNAGSDLASLQTRAESDGDD
YIINGQKIWTSYANYADWIFCLVRTDPAAKKHDGISFILFDMTSKGVSTKPILLISGRSPFCETFFDNVRVPKANVLGTV
NRGWDVAKYLLQHERAMISGMGERGGSRPLGQVAADSVGSDAQGKLDDALLRAQIATFDVDEAALAAAAERMVDLAKAGQ
GHPAFSSAMKYYGTELNKRRHEILMSAGGIDALEWESERSGEGARPRAWLRTKANSIEGGTSEVMLGIVAKRILDLPGA
>Mature_399_residues
MVDSKLADLDTFRRETRAWLEANCPPEMRRPMTSDEDTFWGGRNAKFSSEPQRIWFERMRDKGWTVPHWPKEYGGGGLDG
EEAKIVRQEMAAIGARAPITSFGISMLGPALLKYGTDAQKREHLPKITAGLIRWCQGYSEPNAGSDLASLQTRAESDGDD
YIINGQKIWTSYANYADWIFCLVRTDPAAKKHDGISFILFDMTSKGVSTKPILLISGRSPFCETFFDNVRVPKANVLGTV
NRGWDVAKYLLQHERAMISGMGERGGSRPLGQVAADSVGSDAQGKLDDALLRAQIATFDVDEAALAAAAERMVDLAKAGQ
GHPAFSSAMKYYGTELNKRRHEILMSAGGIDALEWESERSGEGARPRAWLRTKANSIEGGTSEVMLGIVAKRILDLPGA

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI226958414, Length=217, Percent_Identity=34.1013824884793, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI226958412, Length=195, Percent_Identity=34.8717948717949, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI187960098, Length=256, Percent_Identity=30.078125, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4557231, Length=256, Percent_Identity=30.078125, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4501857, Length=398, Percent_Identity=24.1206030150754, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI4501859, Length=180, Percent_Identity=33.8888888888889, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI4557235, Length=155, Percent_Identity=36.1290322580645, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI76496475, Length=155, Percent_Identity=36.1290322580645, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI4557233, Length=266, Percent_Identity=29.6992481203008, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI21361497, Length=224, Percent_Identity=29.0178571428571, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI48976061, Length=188, Percent_Identity=29.7872340425532, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI211971008, Length=188, Percent_Identity=29.7872340425532, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17538396, Length=227, Percent_Identity=30.8370044052863, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17508101, Length=254, Percent_Identity=29.1338582677165, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17505929, Length=217, Percent_Identity=31.3364055299539, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17570075, Length=378, Percent_Identity=26.1904761904762, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17534899, Length=241, Percent_Identity=31.5352697095436, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17551932, Length=217, Percent_Identity=29.9539170506912, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17506239, Length=207, Percent_Identity=32.3671497584541, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71990804, Length=199, Percent_Identity=31.6582914572864, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI32563615, Length=200, Percent_Identity=33, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17569725, Length=214, Percent_Identity=30.8411214953271, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71985184, Length=154, Percent_Identity=35.0649350649351, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71985192, Length=154, Percent_Identity=35.0649350649351, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71982178, Length=190, Percent_Identity=28.4210526315789, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17533517, Length=190, Percent_Identity=28.4210526315789, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21356377, Length=254, Percent_Identity=33.0708661417323, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24666513, Length=348, Percent_Identity=28.448275862069, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24660351, Length=256, Percent_Identity=33.203125, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI281363737, Length=209, Percent_Identity=32.0574162679426, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21355753, Length=193, Percent_Identity=32.6424870466321, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24646207, Length=192, Percent_Identity=29.6875, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19920834, Length=181, Percent_Identity=31.4917127071823, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 43866; Mature: 43866

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDSKLADLDTFRRETRAWLEANCPPEMRRPMTSDEDTFWGGRNAKFSSEPQRIWFERMR
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
DKGWTVPHWPKEYGGGGLDGEEAKIVRQEMAAIGARAPITSFGISMLGPALLKYGTDAQK
HCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHH
REHLPKITAGLIRWCQGYSEPNAGSDLASLQTRAESDGDDYIINGQKIWTSYANYADWIF
HHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHCEEE
CLVRTDPAAKKHDGISFILFDMTSKGVSTKPILLISGRSPFCETFFDNVRVPKANVLGTV
EEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCHHHEECC
NRGWDVAKYLLQHERAMISGMGERGGSRPLGQVAADSVGSDAQGKLDDALLRAQIATFDV
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
DEAALAAAAERMVDLAKAGQGHPAFSSAMKYYGTELNKRRHEILMSAGGIDALEWESERS
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GEGARPRAWLRTKANSIEGGTSEVMLGIVAKRILDLPGA
CCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVDSKLADLDTFRRETRAWLEANCPPEMRRPMTSDEDTFWGGRNAKFSSEPQRIWFERMR
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
DKGWTVPHWPKEYGGGGLDGEEAKIVRQEMAAIGARAPITSFGISMLGPALLKYGTDAQK
HCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHH
REHLPKITAGLIRWCQGYSEPNAGSDLASLQTRAESDGDDYIINGQKIWTSYANYADWIF
HHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHCEEE
CLVRTDPAAKKHDGISFILFDMTSKGVSTKPILLISGRSPFCETFFDNVRVPKANVLGTV
EEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCHHHEECC
NRGWDVAKYLLQHERAMISGMGERGGSRPLGQVAADSVGSDAQGKLDDALLRAQIATFDV
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
DEAALAAAAERMVDLAKAGQGHPAFSSAMKYYGTELNKRRHEILMSAGGIDALEWESERS
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GEGARPRAWLRTKANSIEGGTSEVMLGIVAKRILDLPGA
CCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]