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The map label for this gene is yafC [H]

Identifier: 146343447

GI number: 146343447

Start: 6938834

End: 6939745

Strand: Reverse

Name: yafC [H]

Synonym: BRADO6676

Alternate gene names: 146343447

Gene position: 6939745-6938834 (Counterclockwise)

Preceding gene: 146343450

Following gene: 146343445

Centisome position: 93.07

GC content: 70.29

Gene sequence:

>912_bases
ATGATGTTGGCCAGCGCGGATCGCGCCCGGGATCTGGAGGTGTTCTGCGCCGTCGCTGACAGCGGCAGCTTCTCGGCGGC
GGGCCGCGGCCTCGGGCTGACGCCGTCGGCGGTCAGCCGCACGCTCGACCGTATCGAGATCAGGCTCGGCGCGCGGCTGC
TCTTGCGGTCGACGCGGGCGCTGACGCTGACGGCTGAGGGGCAGGCCTATCTCGCGGCGGCGCGCCGCATCCTCGCCGAC
CTCAACGAGGCCGAGCAGGCGATCGCCGACCAAAGCGCGCCGCGCGGGCGCATCCGCGTCAGCGCCGCGCTCTCGCATGG
CCGCATCTGCATCGTGCCGCTGCTCGGCGAATTCGTGCGGCGCTATCCGAACATCCTGGTCGACATCAATCTCAGCGACG
GGCTCGTCGACATCGCGGCGGGGCAGGCGGATGTCGCGATCCGCGGCGGTCCGCTGGCCGACAGCGCGCTCACCGCCCGC
CGCCTCAGCGACAACGGCCGCAGCATCGTTGCCTCGCCGGATTATCTCGCGCGCTGCGGCGTGCCGCTGACGCCGGAGGA
TCTGCATCACCACAATTGCCTGAACTACGGCTTCCGCCGCGCCGAGCCGGTGTGGCCGTTCCGCCGCGACGGCGTCGACT
ATGCGCTGAAGCTGCGCGGCGCGATCGAAGCCAACAGCGGCGACACGCTGACCGAACTCGCGCTCGATGGCGTCGGCATC
GTCCGCGTCGGCGATTTCAGCCTTCGCAACGCCGTCGCCGAGGGCCGGCTGGTGCGGCTGCTCGAGGCGTTCAATCCCGG
TGACAGGGAGGTCTTCCACGCCGTGTTCGTCGGCGGCGCCAACACGCCCGCGCGGGTGCGCGTGTTCGTGGATTATCTGG
TCGAGCGGATGAATGTGAAGAAAGACGCGTAG

Upstream 100 bases:

>100_bases
GCGAGATCTCCTGGGCTTGTCGATGCACGCAAAGCTAGACCGCGGCGATCCGCAGTATTAGAGGGTAGTCACGCCAAGGA
TTTTTGATCTGGACGCAAAG

Downstream 100 bases:

>100_bases
ACCGAGGACTTGCCGCCCGCCAGGAGGAGAGAACTCCGGCGGGCGGCCGCGTGTCTGATTGATCAGGCCGGCTGCAGCGT
CAGCTCGACCTTGGGGAAGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MMLASADRARDLEVFCAVADSGSFSAAGRGLGLTPSAVSRTLDRIEIRLGARLLLRSTRALTLTAEGQAYLAAARRILAD
LNEAEQAIADQSAPRGRIRVSAALSHGRICIVPLLGEFVRRYPNILVDINLSDGLVDIAAGQADVAIRGGPLADSALTAR
RLSDNGRSIVASPDYLARCGVPLTPEDLHHHNCLNYGFRRAEPVWPFRRDGVDYALKLRGAIEANSGDTLTELALDGVGI
VRVGDFSLRNAVAEGRLVRLLEAFNPGDREVFHAVFVGGANTPARVRVFVDYLVERMNVKKDA

Sequences:

>Translated_303_residues
MMLASADRARDLEVFCAVADSGSFSAAGRGLGLTPSAVSRTLDRIEIRLGARLLLRSTRALTLTAEGQAYLAAARRILAD
LNEAEQAIADQSAPRGRIRVSAALSHGRICIVPLLGEFVRRYPNILVDINLSDGLVDIAAGQADVAIRGGPLADSALTAR
RLSDNGRSIVASPDYLARCGVPLTPEDLHHHNCLNYGFRRAEPVWPFRRDGVDYALKLRGAIEANSGDTLTELALDGVGI
VRVGDFSLRNAVAEGRLVRLLEAFNPGDREVFHAVFVGGANTPARVRVFVDYLVERMNVKKDA
>Mature_303_residues
MMLASADRARDLEVFCAVADSGSFSAAGRGLGLTPSAVSRTLDRIEIRLGARLLLRSTRALTLTAEGQAYLAAARRILAD
LNEAEQAIADQSAPRGRIRVSAALSHGRICIVPLLGEFVRRYPNILVDINLSDGLVDIAAGQADVAIRGGPLADSALTAR
RLSDNGRSIVASPDYLARCGVPLTPEDLHHHNCLNYGFRRAEPVWPFRRDGVDYALKLRGAIEANSGDTLTELALDGVGI
VRVGDFSLRNAVAEGRLVRLLEAFNPGDREVFHAVFVGGANTPARVRVFVDYLVERMNVKKDA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=36.8600682593857, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI87081978, Length=287, Percent_Identity=32.7526132404181, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=31.0810810810811, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1789440, Length=294, Percent_Identity=29.5918367346939, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787589, Length=287, Percent_Identity=29.2682926829268, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI145693193, Length=306, Percent_Identity=29.0849673202614, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787128, Length=286, Percent_Identity=29.3706293706294, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789173, Length=247, Percent_Identity=32.7935222672065, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=25, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1786448, Length=296, Percent_Identity=26.3513513513513, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790262, Length=196, Percent_Identity=28.0612244897959, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790011, Length=308, Percent_Identity=25, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787806, Length=150, Percent_Identity=30.6666666666667, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790208, Length=169, Percent_Identity=26.6272189349112, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI157672245, Length=171, Percent_Identity=28.6549707602339, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32651; Mature: 32651

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLASADRARDLEVFCAVADSGSFSAAGRGLGLTPSAVSRTLDRIEIRLGARLLLRSTRA
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCE
LTLTAEGQAYLAAARRILADLNEAEQAIADQSAPRGRIRVSAALSHGRICIVPLLGEFVR
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEHHHHHHHH
RYPNILVDINLSDGLVDIAAGQADVAIRGGPLADSALTARRLSDNGRSIVASPDYLARCG
HCCCEEEEEECCCCEEEEECCCCCEEEECCCCCHHHHHHHHHCCCCCEEEECCCHHHHCC
VPLTPEDLHHHNCLNYGFRRAEPVWPFRRDGVDYALKLRGAIEANSGDTLTELALDGVGI
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCE
VRVGDFSLRNAVAEGRLVRLLEAFNPGDREVFHAVFVGGANTPARVRVFVDYLVERMNVK
EEECCHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCC
KDA
CCC
>Mature Secondary Structure
MMLASADRARDLEVFCAVADSGSFSAAGRGLGLTPSAVSRTLDRIEIRLGARLLLRSTRA
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCE
LTLTAEGQAYLAAARRILADLNEAEQAIADQSAPRGRIRVSAALSHGRICIVPLLGEFVR
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEHHHHHHHH
RYPNILVDINLSDGLVDIAAGQADVAIRGGPLADSALTARRLSDNGRSIVASPDYLARCG
HCCCEEEEEECCCCEEEEECCCCCEEEECCCCCHHHHHHHHHCCCCCEEEECCCHHHHCC
VPLTPEDLHHHNCLNYGFRRAEPVWPFRRDGVDYALKLRGAIEANSGDTLTELALDGVGI
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCE
VRVGDFSLRNAVAEGRLVRLLEAFNPGDREVFHAVFVGGANTPARVRVFVDYLVERMNVK
EEECCHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCC
KDA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]