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The map label for this gene is poxB [H]

Identifier: 146343413

GI number: 146343413

Start: 6895160

End: 6896890

Strand: Reverse

Name: poxB [H]

Synonym: BRADO6642

Alternate gene names: 146343413

Gene position: 6896890-6895160 (Counterclockwise)

Preceding gene: 146343418

Following gene: 146343412

Centisome position: 92.49

GC content: 66.38

Gene sequence:

>1731_bases
ATGACCACCATCGCAGATCAGATCGTCGAGACTCTCGAGATTGCCGGCGTGAAGCGCATCTACGGCGTCGTCGGCGACAG
CCTCAACGGGCTGACGGAGTCGCTGCGCGTCCGCAAGACGATCGACTGGGTGCATGTCCGTCATGAGGAAGCCGCAGCGT
TTGCAGCTTCGGGAGAATCGCAGATCACCGGCGGCCTCGCGGTTTGCGCCGGCTCTTGTGGCCCGGGCAATCTTCATCTC
ATCAACGGTCTCTACGACGCGCAACGCAGCCGCACGCCGGTGCTGGCGATCGCCGCGCACATTCCGTCGAGCGAGATCGG
CGGCGGCTACTTCCAGGAGACTCATCCCCAGAATCTGTTCCGCGAATGCAGCGTCTATTGCGAGCTGATCTCCGATCCGA
CGCAGATGCCGTTCGTGCTGGACAACGCGATCCGCGCCGCGATCGGCGAGCGCGGCGTCGCCGTGATCGTCATTCCCGGC
GACGTCGCGCTGAAGGCGGCGCCGAAGCGCGCGCTGCCGCCAGCGATCGGCCTGCGGCCGCCGAAGCCGGTGGTGCGCCC
GGCCGAGCCGGAGCTCGATGCGCTGGCGGCGCTGCTCAATGGCGCTCGGCGTGTGACGTTGTTCTGCGGCCGCGGCTGCG
CCGGCGCGCATGCCGAGCTGATGCAGCTCGCCGAGACGCTGCAAAGCCCGATCGTGCATGCACTCGGCGGCAAGGAGCAT
GTCGAATACGACAATCTCTATGACGTCGGCCTCACCGGCTTCATCGGCTTCTCCTCGGGCTATCACGCCATGCACAGCTG
CGACACGCTGCTGATGCTCGGCACCGATTTTCCCTACAAGCAGTTCATTCCGTCGGACATCAAGATCGCCCAGGTCGACA
TCCGCCCGAGCCAGCTCGGCCGCCGCGCCCGCCTCGACGTCGGCGTGGTCGGCGATGTCGGGGAGACTCTTCGCGCATTG
CTGCCGAAGCTCACGGCGAAGTCCGACCGCGGCCATCTCGACGAGAACCTCAAGCGCTATGTCGCCGCCCGCAAGGGCCT
CGACGATCTCGCCGTCGGCACGCCTGGCCGTCCGCCGATCCATCCGCAATATCTGACGCGGCTGATCAGCGAGAACGCCG
CCGATGATGCCGTCTTCACCTTCGACGTCGGCACTCCCACGATCTGGGCCGCGCGCTACATCGCGATGAACGGCCGCCGC
CGGCTCGTGGGCTCGCTGGTGCATGGCTCGATGGCCAATGCGATGTCGCAAGGCATCGGCGTCCAGGCCGCGCAGGCGGG
GCGGCAGGTGATCTCGTTGTCGGGCGACGGCGGCTTCGCGATGCTGATGGGCGATCTGATCACGCTCAAGCAGATGAAGC
TGCCCGTGAAGGTCGCGATCTACAACAACGGCACGCTCGGCTTCGTCGCATTGGAGATGAAGGCGTCCGGCTTCGTCGAG
CACGGCACCGATCTGGAGAATCCGGATTTCGCCGCGATGGCCAAGGCGATGGGCATCCATGGCCGCCGCGTCGAGGATCC
CGGCGAGCTGCCGGGCGCGGTCGCCGAATGGCTCGCGCATGATGGCCCCGCGGTGCTCGACGTCGTCACCGCCAAGCAGG
AGCTGTCGATGCCGCCGACCATCGGCCTGGAACAGGTCAAGGGCTTCAGCGTCTGGCTGATCCGTGCCGTCATGAGCGGC
CGCGGCGACGAGGTGATCGACCTGGCAAAAGAAAATCTGCTGCCGCGATGA

Upstream 100 bases:

>100_bases
CACGCGGTTTTGATTGGGTTGGCTTGGCTTATGCAAGGTACGAGACCTGATCGACCCCGCCGGACTACGCGGTCCTTCAT
GATCAAGGAATCATCAGCTC

Downstream 100 bases:

>100_bases
GGGAAAGATTGCGCCGCGGACGATGCCTGTGGCCCGAAGGCCCGCGATGTCCCCTCATGGTGAGGAGCGCCGCGCCCGAG
TTGAACTTTTGCGACGCGTG

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MTTIADQIVETLEIAGVKRIYGVVGDSLNGLTESLRVRKTIDWVHVRHEEAAAFAASGESQITGGLAVCAGSCGPGNLHL
INGLYDAQRSRTPVLAIAAHIPSSEIGGGYFQETHPQNLFRECSVYCELISDPTQMPFVLDNAIRAAIGERGVAVIVIPG
DVALKAAPKRALPPAIGLRPPKPVVRPAEPELDALAALLNGARRVTLFCGRGCAGAHAELMQLAETLQSPIVHALGGKEH
VEYDNLYDVGLTGFIGFSSGYHAMHSCDTLLMLGTDFPYKQFIPSDIKIAQVDIRPSQLGRRARLDVGVVGDVGETLRAL
LPKLTAKSDRGHLDENLKRYVAARKGLDDLAVGTPGRPPIHPQYLTRLISENAADDAVFTFDVGTPTIWAARYIAMNGRR
RLVGSLVHGSMANAMSQGIGVQAAQAGRQVISLSGDGGFAMLMGDLITLKQMKLPVKVAIYNNGTLGFVALEMKASGFVE
HGTDLENPDFAAMAKAMGIHGRRVEDPGELPGAVAEWLAHDGPAVLDVVTAKQELSMPPTIGLEQVKGFSVWLIRAVMSG
RGDEVIDLAKENLLPR

Sequences:

>Translated_576_residues
MTTIADQIVETLEIAGVKRIYGVVGDSLNGLTESLRVRKTIDWVHVRHEEAAAFAASGESQITGGLAVCAGSCGPGNLHL
INGLYDAQRSRTPVLAIAAHIPSSEIGGGYFQETHPQNLFRECSVYCELISDPTQMPFVLDNAIRAAIGERGVAVIVIPG
DVALKAAPKRALPPAIGLRPPKPVVRPAEPELDALAALLNGARRVTLFCGRGCAGAHAELMQLAETLQSPIVHALGGKEH
VEYDNLYDVGLTGFIGFSSGYHAMHSCDTLLMLGTDFPYKQFIPSDIKIAQVDIRPSQLGRRARLDVGVVGDVGETLRAL
LPKLTAKSDRGHLDENLKRYVAARKGLDDLAVGTPGRPPIHPQYLTRLISENAADDAVFTFDVGTPTIWAARYIAMNGRR
RLVGSLVHGSMANAMSQGIGVQAAQAGRQVISLSGDGGFAMLMGDLITLKQMKLPVKVAIYNNGTLGFVALEMKASGFVE
HGTDLENPDFAAMAKAMGIHGRRVEDPGELPGAVAEWLAHDGPAVLDVVTAKQELSMPPTIGLEQVKGFSVWLIRAVMSG
RGDEVIDLAKENLLPR
>Mature_575_residues
TTIADQIVETLEIAGVKRIYGVVGDSLNGLTESLRVRKTIDWVHVRHEEAAAFAASGESQITGGLAVCAGSCGPGNLHLI
NGLYDAQRSRTPVLAIAAHIPSSEIGGGYFQETHPQNLFRECSVYCELISDPTQMPFVLDNAIRAAIGERGVAVIVIPGD
VALKAAPKRALPPAIGLRPPKPVVRPAEPELDALAALLNGARRVTLFCGRGCAGAHAELMQLAETLQSPIVHALGGKEHV
EYDNLYDVGLTGFIGFSSGYHAMHSCDTLLMLGTDFPYKQFIPSDIKIAQVDIRPSQLGRRARLDVGVVGDVGETLRALL
PKLTAKSDRGHLDENLKRYVAARKGLDDLAVGTPGRPPIHPQYLTRLISENAADDAVFTFDVGTPTIWAARYIAMNGRRR
LVGSLVHGSMANAMSQGIGVQAAQAGRQVISLSGDGGFAMLMGDLITLKQMKLPVKVAIYNNGTLGFVALEMKASGFVEH
GTDLENPDFAAMAKAMGIHGRRVEDPGELPGAVAEWLAHDGPAVLDVVTAKQELSMPPTIGLEQVKGFSVWLIRAVMSGR
GDEVIDLAKENLLPR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=598, Percent_Identity=24.0802675585284, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1787096, Length=572, Percent_Identity=60.3146853146853, Blast_Score=695, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=533, Percent_Identity=27.5797373358349, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1790104, Length=551, Percent_Identity=27.2232304900181, Blast_Score=175, Evalue=7e-45,
Organism=Escherichia coli, GI1786717, Length=491, Percent_Identity=24.2362525458248, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1788716, Length=473, Percent_Identity=22.8329809725159, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17531301, Length=587, Percent_Identity=25.7240204429302, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531299, Length=587, Percent_Identity=25.7240204429302, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=569, Percent_Identity=23.5500878734622, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6320816, Length=502, Percent_Identity=23.1075697211155, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922626, Length=555, Percent_Identity=24.8648648648649, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 61477; Mature: 61345

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIADQIVETLEIAGVKRIYGVVGDSLNGLTESLRVRKTIDWVHVRHEEAAAFAASGES
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHCCCCC
QITGGLAVCAGSCGPGNLHLINGLYDAQRSRTPVLAIAAHIPSSEIGGGYFQETHPQNLF
CCCCCEEEEECCCCCCCEEEECCHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHH
RECSVYCELISDPTQMPFVLDNAIRAAIGERGVAVIVIPGDVALKAAPKRALPPAIGLRP
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCCCCCCC
PKPVVRPAEPELDALAALLNGARRVTLFCGRGCAGAHAELMQLAETLQSPIVHALGGKEH
CCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VEYDNLYDVGLTGFIGFSSGYHAMHSCDTLLMLGTDFPYKQFIPSDIKIAQVDIRPSQLG
CCCCCEEECCCCEEEECCCCCHHHHHCCEEEEECCCCCHHHHCCCCCEEEEEECCHHHHC
RRARLDVGVVGDVGETLRALLPKLTAKSDRGHLDENLKRYVAARKGLDDLAVGTPGRPPI
CHHCCCEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCC
HPQYLTRLISENAADDAVFTFDVGTPTIWAARYIAMNGRRRLVGSLVHGSMANAMSQGIG
CHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
VQAAQAGRQVISLSGDGGFAMLMGDLITLKQMKLPVKVAIYNNGTLGFVALEMKASGFVE
CHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEEEECCCCHH
HGTDLENPDFAAMAKAMGIHGRRVEDPGELPGAVAEWLAHDGPAVLDVVTAKQELSMPPT
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHCCCCCC
IGLEQVKGFSVWLIRAVMSGRGDEVIDLAKENLLPR
CCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TTIADQIVETLEIAGVKRIYGVVGDSLNGLTESLRVRKTIDWVHVRHEEAAAFAASGES
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHCCCCC
QITGGLAVCAGSCGPGNLHLINGLYDAQRSRTPVLAIAAHIPSSEIGGGYFQETHPQNLF
CCCCCEEEEECCCCCCCEEEECCHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHH
RECSVYCELISDPTQMPFVLDNAIRAAIGERGVAVIVIPGDVALKAAPKRALPPAIGLRP
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCCCCCCC
PKPVVRPAEPELDALAALLNGARRVTLFCGRGCAGAHAELMQLAETLQSPIVHALGGKEH
CCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VEYDNLYDVGLTGFIGFSSGYHAMHSCDTLLMLGTDFPYKQFIPSDIKIAQVDIRPSQLG
CCCCCEEECCCCEEEECCCCCHHHHHCCEEEEECCCCCHHHHCCCCCEEEEEECCHHHHC
RRARLDVGVVGDVGETLRALLPKLTAKSDRGHLDENLKRYVAARKGLDDLAVGTPGRPPI
CHHCCCEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCC
HPQYLTRLISENAADDAVFTFDVGTPTIWAARYIAMNGRRRLVGSLVHGSMANAMSQGIG
CHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
VQAAQAGRQVISLSGDGGFAMLMGDLITLKQMKLPVKVAIYNNGTLGFVALEMKASGFVE
CHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEEEECCCCHH
HGTDLENPDFAAMAKAMGIHGRRVEDPGELPGAVAEWLAHDGPAVLDVVTAKQELSMPPT
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHCCCCCC
IGLEQVKGFSVWLIRAVMSGRGDEVIDLAKENLLPR
CCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]