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The map label for this gene is gltX1 [H]

Identifier: 146343277

GI number: 146343277

Start: 6746156

End: 6747025

Strand: Reverse

Name: gltX1 [H]

Synonym: BRADO6498

Alternate gene names: 146343277

Gene position: 6747025-6746156 (Counterclockwise)

Preceding gene: 146343284

Following gene: 146343276

Centisome position: 90.48

GC content: 66.55

Gene sequence:

>870_bases
ATGCCACCCGTTTTCCGATTTGCCCCCAGCCCCAACGGCTATCTCCATCTCGGCCACGCCTATTCGGCGCTGCTGAACGT
CCAGCGTGCGCGGGAGACCGGTGGCCGCCTGCTGTTGCGGATCGAGGACATCGACCCGACGCGCTGCCGGCCCGAATATG
AGCAGGCGATCACCGAGGATCTCGCCTGGCTCGGCATCGCCTGGGAGCAGCCGGTGCGGCGGCAGTCGCTGCATCTCGCT
GCGTATGGCGCTGCGCTGGAGCGGCTGCAGGATATGGGGCTCGTCTATCCGAGCTTCGAGAGCCGGGCCGAAATCGCGCG
GCTGGTCGCGCAGCGAGGTGTCGAGGGCAATTGGCCGCAGGATCCTGATGGGGCGCCGCTCTATCCCGGTGCGGCAAAGG
CAATGTCTGCAACACGCCGTGCAGAACTGATGAGCAGCGGCCTGCCCTATGCGCTGCGACTCGATATGGAAGCGGCGGTC
GTGCGCGCCGGAGGGCTGAGCTGGGTCGAGGTCGGCTGTGGGCCGAACGGCGAGAGCGGGACGATCGAGGCGACCCCGGC
AGTCTGGGGCGACGTCATCCTGGCGCGCAAGGAAACACCCACCAGCTACCATCTGTCCGTGGTCGTGGACGACGCGCTGC
AAGGCGTGACCGAGGTGGTGCGTGGACAGGATCTGTTTCATGCGACCAGCGTGCACCGGCTGCTGCAGGTCCTGCTCGGC
CTGCCGGCGCCGGCCTATCGGCACCACCAACTGATTCGCGACGATATGGGCCACAAGCTGTCCAAATCCACGCAAGCGAC
GGCTTTGCGCACATTGCGCGCCGAAGGCGTCTCGCCGGCGGAAATCCGACACAGGATCGGCTTGGTATAG

Upstream 100 bases:

>100_bases
GCGCGGGCTTGACCCGCGCATCCATCTCGGATCGAACGTTTTCGCAAAGGAGCATGAGTGTCGGATCACGCGAGCTCAAG
TCCTGCAAGGACGAACTGAC

Downstream 100 bases:

>100_bases
CCGTGTCCTGCCGCAAGTCCCGCTGTCTCCGAATGTTTCCGGCGGGGACAACCTCCGGGGCATCGTGACACTCGGAAACG
ACCGTGCCATGCTGACCAAT

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MPPVFRFAPSPNGYLHLGHAYSALLNVQRARETGGRLLLRIEDIDPTRCRPEYEQAITEDLAWLGIAWEQPVRRQSLHLA
AYGAALERLQDMGLVYPSFESRAEIARLVAQRGVEGNWPQDPDGAPLYPGAAKAMSATRRAELMSSGLPYALRLDMEAAV
VRAGGLSWVEVGCGPNGESGTIEATPAVWGDVILARKETPTSYHLSVVVDDALQGVTEVVRGQDLFHATSVHRLLQVLLG
LPAPAYRHHQLIRDDMGHKLSKSTQATALRTLRAEGVSPAEIRHRIGLV

Sequences:

>Translated_289_residues
MPPVFRFAPSPNGYLHLGHAYSALLNVQRARETGGRLLLRIEDIDPTRCRPEYEQAITEDLAWLGIAWEQPVRRQSLHLA
AYGAALERLQDMGLVYPSFESRAEIARLVAQRGVEGNWPQDPDGAPLYPGAAKAMSATRRAELMSSGLPYALRLDMEAAV
VRAGGLSWVEVGCGPNGESGTIEATPAVWGDVILARKETPTSYHLSVVVDDALQGVTEVVRGQDLFHATSVHRLLQVLLG
LPAPAYRHHQLIRDDMGHKLSKSTQATALRTLRAEGVSPAEIRHRIGLV
>Mature_288_residues
PPVFRFAPSPNGYLHLGHAYSALLNVQRARETGGRLLLRIEDIDPTRCRPEYEQAITEDLAWLGIAWEQPVRRQSLHLAA
YGAALERLQDMGLVYPSFESRAEIARLVAQRGVEGNWPQDPDGAPLYPGAAKAMSATRRAELMSSGLPYALRLDMEAAVV
RAGGLSWVEVGCGPNGESGTIEATPAVWGDVILARKETPTSYHLSVVVDDALQGVTEVVRGQDLFHATSVHRLLQVLLGL
PAPAYRHHQLIRDDMGHKLSKSTQATALRTLRAEGVSPAEIRHRIGLV

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=284, Percent_Identity=32.0422535211268, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788743, Length=276, Percent_Identity=30.7971014492754, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI226510925, Length=264, Percent_Identity=34.469696969697, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786895, Length=91, Percent_Identity=40.6593406593407, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=267, Percent_Identity=28.0898876404494, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6324540, Length=279, Percent_Identity=28.673835125448, Blast_Score=112, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321192, Length=226, Percent_Identity=27.8761061946903, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24665321, Length=285, Percent_Identity=31.2280701754386, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 31629; Mature: 31497

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPVFRFAPSPNGYLHLGHAYSALLNVQRARETGGRLLLRIEDIDPTRCRPEYEQAITED
CCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHH
LAWLGIAWEQPVRRQSLHLAAYGAALERLQDMGLVYPSFESRAEIARLVAQRGVEGNWPQ
HHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
DPDGAPLYPGAAKAMSATRRAELMSSGLPYALRLDMEAAVVRAGGLSWVEVGCGPNGESG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCEEEEECCCCCCCCC
TIEATPAVWGDVILARKETPTSYHLSVVVDDALQGVTEVVRGQDLFHATSVHRLLQVLLG
EEEECCHHHHHEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
LPAPAYRHHQLIRDDMGHKLSKSTQATALRTLRAEGVSPAEIRHRIGLV
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure 
PPVFRFAPSPNGYLHLGHAYSALLNVQRARETGGRLLLRIEDIDPTRCRPEYEQAITED
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHH
LAWLGIAWEQPVRRQSLHLAAYGAALERLQDMGLVYPSFESRAEIARLVAQRGVEGNWPQ
HHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
DPDGAPLYPGAAKAMSATRRAELMSSGLPYALRLDMEAAVVRAGGLSWVEVGCGPNGESG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCEEEEECCCCCCCCC
TIEATPAVWGDVILARKETPTSYHLSVVVDDALQGVTEVVRGQDLFHATSVHRLLQVLLG
EEEECCHHHHHEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
LPAPAYRHHQLIRDDMGHKLSKSTQATALRTLRAEGVSPAEIRHRIGLV
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA