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The map label for this gene is emrA [H]

Identifier: 146342983

GI number: 146342983

Start: 6392604

End: 6393734

Strand: Direct

Name: emrA [H]

Synonym: BRADO6166

Alternate gene names: 146342983

Gene position: 6392604-6393734 (Clockwise)

Preceding gene: 146342981

Following gene: 146342984

Centisome position: 85.73

GC content: 70.38

Gene sequence:

>1131_bases
GTGAGTGAAACGAGCAACGTCGATGCGGCGTCGCGCGCGGCAGCGCCGCAACCGCCCTCGCCCGCTGCGTCGCCCGGCCT
CTGGGCGCGCCTCGCCATTCCGCTGCTGGCCGTCATCGTTGCGTTGGGGTTCGTGGCGCTCGCGACGACGCGCTGGGACG
CCTGGACCGGCAACGCCACGACGCAGTCGACCAACGACGCCTATGTCCGCGCCGACCTCACCCAGCTCTCGAGCCGCGTC
GCCGGCGAGGTGCTGAAGGTCGCGGTCAGCGATTTCCAGCAGGTCAAGGCCGGCGACATCCTGATCCAGATCGATCCGGC
CGACTACGAGGCGCAGGTCGCGCAGGCCGAGGCCAATGTCGCGGCGGCACAGGCGGCGCTTGACAATCTCGGCAACCAGA
TCGAGCTGCAATACGCGACCATCGCCCAGGCCGAGGCGCAGCAGGTCTCGGCGGGCGCCGCCGAGGTGCAGGCGCGGCAG
GAGGAGGAGCGCCAGCAGTCGCTGTCGCAGACCGAGGCCGGCACGCGGCAACGGCTCGAGCAAGCCACCGCCGCCTATGC
CAAGGCGCAGGCAGACGTGCGCGCCAGCCGCGCCGTCATCGCCGCGCAGCGCCATCAGCTGGAGGTGCTCACCGGCACCA
AGACGCAGCGCGGCGCCGACCTCGCCGGCGCCAAAGCGGCGCTGGCCGCCGCCAGGCTGAAGCTCGGCTACACCAAGATC
ACGGCGCCGTTCGACGGAGTCGTCGGTCAGCGCCAGGTGCAGACCGGCGACTACGTCAACATCGGCAGCAGCCTGATCGC
CGTGGTGCCGCTGCCGCAGGTCTTCATCATCGCGAATTACAAGGAGACCCAGCTCACGCATGTCGCGCCCGGTCAGCCGG
TCGACGTCACCGTGGACTCGTTTCCGGGCGAGAAGCTGCACGGCCGGGTCGAGCGGATCGCGCCGGCCTCCGGCTCGCAA
TTCGCGCTGCTGCCGCCTGACAATGCGACCGGCAATTTCACCAAGGTCGTGCAGCGCATTCCCGTGCGCATCGCGCTCGA
TGCCGATCAGCCGTTGCTCGCGCGCCTGCTGCCGGGCATGTCGGTGGTGACCCACATCCGCACCGCGGGCGCTGCCGATG
GCGGCAAGTGA

Upstream 100 bases:

>100_bases
GGTGTGCGCGCGCAGGCGCCATTCCGGAGTCGAGGTGGCGCGGGACGCAGAATGAGGTAGCAATAGTCCGGCGTCGAAAC
GTGATGCAGGAGGGCGCATC

Downstream 100 bases:

>100_bases
CCCGACGAGCGAGAGCATCAGCGCCGCCGATCGCGGACCGCTGTCGCGCGGCGGCGTCGCGCCGCAGCCGCTGTTCGCGG
TCGGCGCCGTGCTGCTCGGC

Product: HlyD family secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSETSNVDAASRAAAPQPPSPAASPGLWARLAIPLLAVIVALGFVALATTRWDAWTGNATTQSTNDAYVRADLTQLSSRV
AGEVLKVAVSDFQQVKAGDILIQIDPADYEAQVAQAEANVAAAQAALDNLGNQIELQYATIAQAEAQQVSAGAAEVQARQ
EEERQQSLSQTEAGTRQRLEQATAAYAKAQADVRASRAVIAAQRHQLEVLTGTKTQRGADLAGAKAALAAARLKLGYTKI
TAPFDGVVGQRQVQTGDYVNIGSSLIAVVPLPQVFIIANYKETQLTHVAPGQPVDVTVDSFPGEKLHGRVERIAPASGSQ
FALLPPDNATGNFTKVVQRIPVRIALDADQPLLARLLPGMSVVTHIRTAGAADGGK

Sequences:

>Translated_376_residues
MSETSNVDAASRAAAPQPPSPAASPGLWARLAIPLLAVIVALGFVALATTRWDAWTGNATTQSTNDAYVRADLTQLSSRV
AGEVLKVAVSDFQQVKAGDILIQIDPADYEAQVAQAEANVAAAQAALDNLGNQIELQYATIAQAEAQQVSAGAAEVQARQ
EEERQQSLSQTEAGTRQRLEQATAAYAKAQADVRASRAVIAAQRHQLEVLTGTKTQRGADLAGAKAALAAARLKLGYTKI
TAPFDGVVGQRQVQTGDYVNIGSSLIAVVPLPQVFIIANYKETQLTHVAPGQPVDVTVDSFPGEKLHGRVERIAPASGSQ
FALLPPDNATGNFTKVVQRIPVRIALDADQPLLARLLPGMSVVTHIRTAGAADGGK
>Mature_375_residues
SETSNVDAASRAAAPQPPSPAASPGLWARLAIPLLAVIVALGFVALATTRWDAWTGNATTQSTNDAYVRADLTQLSSRVA
GEVLKVAVSDFQQVKAGDILIQIDPADYEAQVAQAEANVAAAQAALDNLGNQIELQYATIAQAEAQQVSAGAAEVQARQE
EERQQSLSQTEAGTRQRLEQATAAYAKAQADVRASRAVIAAQRHQLEVLTGTKTQRGADLAGAKAALAAARLKLGYTKIT
APFDGVVGQRQVQTGDYVNIGSSLIAVVPLPQVFIIANYKETQLTHVAPGQPVDVTVDSFPGEKLHGRVERIAPASGSQF
ALLPPDNATGNFTKVVQRIPVRIALDADQPLLARLLPGMSVVTHIRTAGAADGGK

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=341, Percent_Identity=33.7243401759531, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1789041, Length=313, Percent_Identity=33.5463258785943, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790519, Length=321, Percent_Identity=30.5295950155763, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87081951, Length=300, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789637, Length=331, Percent_Identity=28.0966767371601, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790012, Length=246, Percent_Identity=28.0487804878049, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790024, Length=250, Percent_Identity=25.6, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082036, Length=227, Percent_Identity=26.8722466960352, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787013, Length=287, Percent_Identity=29.9651567944251, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39416; Mature: 39285

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETSNVDAASRAAAPQPPSPAASPGLWARLAIPLLAVIVALGFVALATTRWDAWTGNAT
CCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TQSTNDAYVRADLTQLSSRVAGEVLKVAVSDFQQVKAGDILIQIDPADYEAQVAQAEANV
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
AAAQAALDNLGNQIELQYATIAQAEAQQVSAGAAEVQARQEEERQQSLSQTEAGTRQRLE
HHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QATAAYAKAQADVRASRAVIAAQRHQLEVLTGTKTQRGADLAGAKAALAAARLKLGYTKI
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEE
TAPFDGVVGQRQVQTGDYVNIGSSLIAVVPLPQVFIIANYKETQLTHVAPGQPVDVTVDS
CCCCCCCCCCCEECCCCEEECCCCEEEEECCCEEEEEECCCCCEEEECCCCCCEEEEECC
FPGEKLHGRVERIAPASGSQFALLPPDNATGNFTKVVQRIPVRIALDADQPLLARLLPGM
CCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHCCCH
SVVTHIRTAGAADGGK
HHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SETSNVDAASRAAAPQPPSPAASPGLWARLAIPLLAVIVALGFVALATTRWDAWTGNAT
CCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TQSTNDAYVRADLTQLSSRVAGEVLKVAVSDFQQVKAGDILIQIDPADYEAQVAQAEANV
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
AAAQAALDNLGNQIELQYATIAQAEAQQVSAGAAEVQARQEEERQQSLSQTEAGTRQRLE
HHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QATAAYAKAQADVRASRAVIAAQRHQLEVLTGTKTQRGADLAGAKAALAAARLKLGYTKI
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEE
TAPFDGVVGQRQVQTGDYVNIGSSLIAVVPLPQVFIIANYKETQLTHVAPGQPVDVTVDS
CCCCCCCCCCCEECCCCEEECCCCEEEEECCCEEEEEECCCCCEEEECCCCCCEEEEECC
FPGEKLHGRVERIAPASGSQFALLPPDNATGNFTKVVQRIPVRIALDADQPLLARLLPGM
CCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHCCCH
SVVTHIRTAGAADGGK
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]