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The map label for this gene is emrB [H]

Identifier: 146342984

GI number: 146342984

Start: 6393721

End: 6395370

Strand: Direct

Name: emrB [H]

Synonym: BRADO6167

Alternate gene names: 146342984

Gene position: 6393721-6395370 (Clockwise)

Preceding gene: 146342983

Following gene: 146342985

Centisome position: 85.75

GC content: 67.52

Gene sequence:

>1650_bases
ATGGCGGCAAGTGACCCGACGAGCGAGAGCATCAGCGCCGCCGATCGCGGACCGCTGTCGCGCGGCGGCGTCGCGCCGCA
GCCGCTGTTCGCGGTCGGCGCCGTGCTGCTCGGCTCCTTCCTCGCCAATTTCGACAGCCGTCTCACCTCGGTCGGTTTGC
CCGATCTGCGCGGCGCGTTCGGGCTCGGCTTCGACCAGGGCGCGTGGCTGTCGACCGCCGCGATCGGCTCGCAGATCTTC
ATCGCGCCCGCCGTGGCCTGGCTCGCCACGGCGTTCGGCCTGCGCCGCGTGCTCGGCGTTCCGAGCCTGATCTATGCCGC
GGTGTCGCTCATCATTCCGTTCGTGCGCGACTACCCGACCCTGATCACGCTCGCGATCATCCACGGCATGCTGCTCGGCA
CCTTCGTGCCGGCAACCTTGATGATCGTCTTCCGCAACCTGCCGATCCGCTGGTGGCTGCCGGCAATCTCGATCTACTCG
ATCCGCGTCGGCGTCGCACTGGACACCTCGACGTCGCTGGTCGGCTTCTATGTCGAGCATCTCGGCTGGCAGTGGCTGTA
CTGGCAGGGCGTCGTGCTGGCCCCCCTGATGGCACTGATGGTCTATCTCGGGACGCCGAGCGAACCGGTCAATCGCACGC
TGCTCGAGAACGCCGACTGGGGCGGCATGCTGCTGCTCGGCAGCGCCGTGTCGATGATCTATGCCGGCCTCGATCAGGGC
AACCGGCTCGACTGGCTGCAATCCGGCACGGTGGTGGCGCTGCTGGCCGGCGGCGCCGTGCTGTTCGTGCTGTTTCTCGT
CAACGAGGCGCTGGCGCCGCAGCCATGGGCGCATGTCAACGTGCTGCTTTCGCGCAACATCGGGCTCTCGCTGGCGGTCA
TTCTGCTGTACACGCTGACCAGCCTGTCGAACGCGTCGCTGGTGCCGAACTTCCTCGCTACGATCACGCAACTGCGCCCC
GAGCAGAGCGGCTCGCTGCTGTTGCTCTATGGCGCCTTGCCGATGCTCGTGCTGGTCCCGCTCTCGATCGCGATGCTGCG
CCATCTCGATCCCCGCATCGTCGTCGTGTTCGGCCTCAGCGCGTTCGCCGCGGCCAATCTGATGGGCACGCAGCTGACCC
ACGCCTGGGCGCGCGAGGACTTCATCGTCATCGTGGTACTGCAATCGATTGGTCAAGCGTTGACCCTGCTGCCGATCATC
ATTTTGGCGCTGTCCAACTCCGATCCGGCGCGCGCGACCTCGTTCGCCGCCTACATCCAGATCATGCGGCTCGGCGGCGC
CGAGATCGGCGTCGCGCTGATGGGCACGTGGCTGCGGGTGCGCGAGCAAATGCATTCGGCCACTCTCGGCCTCAACATCC
AGCGCGGCGACGGCGATGTCGCCCGCGCGCTGCAGCGGCTGACCGGCTTTTTCACCGCCCACGGCGGCGCCCAGGCCAAG
GCCCGCGCGATCGGGACGCTGGCCGCCGAGGTCCAGCGCGAGGCCAATGTGCTGGCCTATCTCGATGCGTTCTGGCTATG
CTTCTGGCTCGCGATCACCGCACTCGGGCTGGTCGCATTGATCACGCGCGCGCCGCCGGGTCCGTTCTCCCCGACCCCCG
TCCGCTTCGGCGCAGGCTGGGTGAAGCGCATCGCCGCAAGATTCACATAA

Upstream 100 bases:

>100_bases
ATTCCCGTGCGCATCGCGCTCGATGCCGATCAGCCGTTGCTCGCGCGCCTGCTGCCGGGCATGTCGGTGGTGACCCACAT
CCGCACCGCGGGCGCTGCCG

Downstream 100 bases:

>100_bases
GATAATCTCCGGGCCAGCGTGACTAACAACTAATCATCTGTTGCTTATTCGCGACAGACGGATCCGCGAGCAGTTGATCA
AATGATGATCATCCTCGCAT

Product: putative transport protein permease

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MAASDPTSESISAADRGPLSRGGVAPQPLFAVGAVLLGSFLANFDSRLTSVGLPDLRGAFGLGFDQGAWLSTAAIGSQIF
IAPAVAWLATAFGLRRVLGVPSLIYAAVSLIIPFVRDYPTLITLAIIHGMLLGTFVPATLMIVFRNLPIRWWLPAISIYS
IRVGVALDTSTSLVGFYVEHLGWQWLYWQGVVLAPLMALMVYLGTPSEPVNRTLLENADWGGMLLLGSAVSMIYAGLDQG
NRLDWLQSGTVVALLAGGAVLFVLFLVNEALAPQPWAHVNVLLSRNIGLSLAVILLYTLTSLSNASLVPNFLATITQLRP
EQSGSLLLLYGALPMLVLVPLSIAMLRHLDPRIVVVFGLSAFAAANLMGTQLTHAWAREDFIVIVVLQSIGQALTLLPII
ILALSNSDPARATSFAAYIQIMRLGGAEIGVALMGTWLRVREQMHSATLGLNIQRGDGDVARALQRLTGFFTAHGGAQAK
ARAIGTLAAEVQREANVLAYLDAFWLCFWLAITALGLVALITRAPPGPFSPTPVRFGAGWVKRIAARFT

Sequences:

>Translated_549_residues
MAASDPTSESISAADRGPLSRGGVAPQPLFAVGAVLLGSFLANFDSRLTSVGLPDLRGAFGLGFDQGAWLSTAAIGSQIF
IAPAVAWLATAFGLRRVLGVPSLIYAAVSLIIPFVRDYPTLITLAIIHGMLLGTFVPATLMIVFRNLPIRWWLPAISIYS
IRVGVALDTSTSLVGFYVEHLGWQWLYWQGVVLAPLMALMVYLGTPSEPVNRTLLENADWGGMLLLGSAVSMIYAGLDQG
NRLDWLQSGTVVALLAGGAVLFVLFLVNEALAPQPWAHVNVLLSRNIGLSLAVILLYTLTSLSNASLVPNFLATITQLRP
EQSGSLLLLYGALPMLVLVPLSIAMLRHLDPRIVVVFGLSAFAAANLMGTQLTHAWAREDFIVIVVLQSIGQALTLLPII
ILALSNSDPARATSFAAYIQIMRLGGAEIGVALMGTWLRVREQMHSATLGLNIQRGDGDVARALQRLTGFFTAHGGAQAK
ARAIGTLAAEVQREANVLAYLDAFWLCFWLAITALGLVALITRAPPGPFSPTPVRFGAGWVKRIAARFT
>Mature_548_residues
AASDPTSESISAADRGPLSRGGVAPQPLFAVGAVLLGSFLANFDSRLTSVGLPDLRGAFGLGFDQGAWLSTAAIGSQIFI
APAVAWLATAFGLRRVLGVPSLIYAAVSLIIPFVRDYPTLITLAIIHGMLLGTFVPATLMIVFRNLPIRWWLPAISIYSI
RVGVALDTSTSLVGFYVEHLGWQWLYWQGVVLAPLMALMVYLGTPSEPVNRTLLENADWGGMLLLGSAVSMIYAGLDQGN
RLDWLQSGTVVALLAGGAVLFVLFLVNEALAPQPWAHVNVLLSRNIGLSLAVILLYTLTSLSNASLVPNFLATITQLRPE
QSGSLLLLYGALPMLVLVPLSIAMLRHLDPRIVVVFGLSAFAAANLMGTQLTHAWAREDFIVIVVLQSIGQALTLLPIII
LALSNSDPARATSFAAYIQIMRLGGAEIGVALMGTWLRVREQMHSATLGLNIQRGDGDVARALQRLTGFFTAHGGAQAKA
RAIGTLAAEVQREANVLAYLDAFWLCFWLAITALGLVALITRAPPGPFSPTPVRFGAGWVKRIAARFT

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=491, Percent_Identity=22.6069246435845, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788710, Length=484, Percent_Identity=21.0743801652893, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58665; Mature: 58534

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS00213 LIPOCALIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASDPTSESISAADRGPLSRGGVAPQPLFAVGAVLLGSFLANFDSRLTSVGLPDLRGAF
CCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GLGFDQGAWLSTAAIGSQIFIAPAVAWLATAFGLRRVLGVPSLIYAAVSLIIPFVRDYPT
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LITLAIIHGMLLGTFVPATLMIVFRNLPIRWWLPAISIYSIRVGVALDTSTSLVGFYVEH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHEEEEEEEEEEECCCHHHHHHHHHH
LGWQWLYWQGVVLAPLMALMVYLGTPSEPVNRTLLENADWGGMLLLGSAVSMIYAGLDQG
CCCEEEHHCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
NRLDWLQSGTVVALLAGGAVLFVLFLVNEALAPQPWAHVNVLLSRNIGLSLAVILLYTLT
CCCCHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHEEEEEECCCCHHHHHHHHHHHH
SLSNASLVPNFLATITQLRPEQSGSLLLLYGALPMLVLVPLSIAMLRHLDPRIVVVFGLS
HCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHH
AFAAANLMGTQLTHAWAREDFIVIVVLQSIGQALTLLPIIILALSNSDPARATSFAAYIQ
HHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
IMRLGGAEIGVALMGTWLRVREQMHSATLGLNIQRGDGDVARALQRLTGFFTAHGGAQAK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHCCCCCHHH
ARAIGTLAAEVQREANVLAYLDAFWLCFWLAITALGLVALITRAPPGPFSPTPVRFGAGW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
VKRIAARFT
HHHHHHHCC
>Mature Secondary Structure 
AASDPTSESISAADRGPLSRGGVAPQPLFAVGAVLLGSFLANFDSRLTSVGLPDLRGAF
CCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GLGFDQGAWLSTAAIGSQIFIAPAVAWLATAFGLRRVLGVPSLIYAAVSLIIPFVRDYPT
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LITLAIIHGMLLGTFVPATLMIVFRNLPIRWWLPAISIYSIRVGVALDTSTSLVGFYVEH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHEEEEEEEEEEECCCHHHHHHHHHH
LGWQWLYWQGVVLAPLMALMVYLGTPSEPVNRTLLENADWGGMLLLGSAVSMIYAGLDQG
CCCEEEHHCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
NRLDWLQSGTVVALLAGGAVLFVLFLVNEALAPQPWAHVNVLLSRNIGLSLAVILLYTLT
CCCCHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHEEEEEECCCCHHHHHHHHHHHH
SLSNASLVPNFLATITQLRPEQSGSLLLLYGALPMLVLVPLSIAMLRHLDPRIVVVFGLS
HCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHH
AFAAANLMGTQLTHAWAREDFIVIVVLQSIGQALTLLPIIILALSNSDPARATSFAAYIQ
HHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
IMRLGGAEIGVALMGTWLRVREQMHSATLGLNIQRGDGDVARALQRLTGFFTAHGGAQAK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHCCCCCHHH
ARAIGTLAAEVQREANVLAYLDAFWLCFWLAITALGLVALITRAPPGPFSPTPVRFGAGW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
VKRIAARFT
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]