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The map label for this gene is livJ [C]

Identifier: 146342976

GI number: 146342976

Start: 6384463

End: 6385650

Strand: Direct

Name: livJ [C]

Synonym: BRADO6159

Alternate gene names: 146342976

Gene position: 6384463-6385650 (Clockwise)

Preceding gene: 146342975

Following gene: 146342978

Centisome position: 85.62

GC content: 65.82

Gene sequence:

>1188_bases
GTGCCGAAGTTCGACACGCGGATGGCCAATGCGGCCGTGGCCGCGCAGGCCAGCGCGCAGCCCGCGGTCCAGGGCGTGAC
CGACACCGAGATCCGCTTCGGCATGGCCGCTCCGTTCTCGGGCCCAGCCCGGGAGATGGGCCGCCAGCTCAAGCTCGGCA
TCGAGCTCGCCTTCGCGCAGGTCAATGAGGACGGCGGCGTGCATGGCCGTCAGCTCAAGCTCACCACGGCCGACGATGCC
TACGAGCCGACGCGAACGCTGAATGCGATGAAGGACCTCTACGAGCAGCAGAAGGTGTTCGGCTTCGTGGAGAACTATGG
CAGCCCGACCGCGCTGATCTCCGTCCCCTACGCGCTCGAGCGCCATGCGTTGTATTTCGGCGCCTTCACCGGCGCGCCCT
CGCTGCGACGGGATCCGCCCGACCGCTACGTCTTCAACTACCGGGCTGGCTATGCCGAGGAGGCCGACGCGATCGTGCGC
TATCTCGTCAAGAACCGCCGGCTGCGTCCGCAGGACATCGGCGTACTCACCCAGCAGGACGCCTATGGCGATGCCGGCTG
GGACGGCGTTGTCAAGGCGATGCGCGGGCTGCGCGGCGGCAATGCCGGCGAGATCTTCCGCATGAGCTACAACCGCAACA
CCGTCAATGTCGACGAGGCCATCGCGCAGCTGAAGCGGCAGCGGCCTGTCATCAAGGCCGTCGTCCTCGTCGCCACCTTC
CGGGCCGCTGCGAAGTTCATCGAAAAGACCCGCGACCTCTATCCCGACATGATCTACGCGACGATCTCCGGCGTCGGCAG
CACGGGGCTCGCCGGCGAGCTCATGGTGCTCGGACCCAAATATGCCGATGGCGTGATCTGCACCCAGGTCACCCCGGCCG
TCGACGGCTACGCCTCTGTCACGCTGAGTTACAAGAAGGCGCTCGCCAAGTATTTTCCGGGCGAGCCGGCCGACTACACA
TCGCTGGAAGCCTATCTCACGGCCAATATCCTGATCGAGGGGCTGCGACGGACTGGTCCCGCGCTCGATACCGAGTCGCT
GGTCACCAATCTCGAGGCGATGCAGAACTACGAGCTCGGCGTCGGCTCCAAGGTGAGCTTCGGCCCCTCCGACCACCAGG
CGCTGCACAAAGTCTGGGGCACCCAGCTCGACAAGACCGGGCAGTATCACGCGATCGATCTGGACTAG

Upstream 100 bases:

>100_bases
CGCTTCGCAGGCGTTCGCCCAGGTGCAAGACGCGCCATCGGCGAACGCCGCTCCCGCGATTGCTCCCGATGCTGGCGCGC
GCCTGAGGGAGGAGGACCGT

Downstream 100 bases:

>100_bases
CAGGTCCGGCCGACGTCCATCGGGCGGCACGGCAGCCATGGCGAGGGGCGGTCAGCGCCGATCTCGAATGACGCTTCTCT
TGGCGCCGGCGGCCTACTTC

Product: putative branched-chain amino acid ABC transporter substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: None

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MPKFDTRMANAAVAAQASAQPAVQGVTDTEIRFGMAAPFSGPAREMGRQLKLGIELAFAQVNEDGGVHGRQLKLTTADDA
YEPTRTLNAMKDLYEQQKVFGFVENYGSPTALISVPYALERHALYFGAFTGAPSLRRDPPDRYVFNYRAGYAEEADAIVR
YLVKNRRLRPQDIGVLTQQDAYGDAGWDGVVKAMRGLRGGNAGEIFRMSYNRNTVNVDEAIAQLKRQRPVIKAVVLVATF
RAAAKFIEKTRDLYPDMIYATISGVGSTGLAGELMVLGPKYADGVICTQVTPAVDGYASVTLSYKKALAKYFPGEPADYT
SLEAYLTANILIEGLRRTGPALDTESLVTNLEAMQNYELGVGSKVSFGPSDHQALHKVWGTQLDKTGQYHAIDLD

Sequences:

>Translated_395_residues
MPKFDTRMANAAVAAQASAQPAVQGVTDTEIRFGMAAPFSGPAREMGRQLKLGIELAFAQVNEDGGVHGRQLKLTTADDA
YEPTRTLNAMKDLYEQQKVFGFVENYGSPTALISVPYALERHALYFGAFTGAPSLRRDPPDRYVFNYRAGYAEEADAIVR
YLVKNRRLRPQDIGVLTQQDAYGDAGWDGVVKAMRGLRGGNAGEIFRMSYNRNTVNVDEAIAQLKRQRPVIKAVVLVATF
RAAAKFIEKTRDLYPDMIYATISGVGSTGLAGELMVLGPKYADGVICTQVTPAVDGYASVTLSYKKALAKYFPGEPADYT
SLEAYLTANILIEGLRRTGPALDTESLVTNLEAMQNYELGVGSKVSFGPSDHQALHKVWGTQLDKTGQYHAIDLD
>Mature_394_residues
PKFDTRMANAAVAAQASAQPAVQGVTDTEIRFGMAAPFSGPAREMGRQLKLGIELAFAQVNEDGGVHGRQLKLTTADDAY
EPTRTLNAMKDLYEQQKVFGFVENYGSPTALISVPYALERHALYFGAFTGAPSLRRDPPDRYVFNYRAGYAEEADAIVRY
LVKNRRLRPQDIGVLTQQDAYGDAGWDGVVKAMRGLRGGNAGEIFRMSYNRNTVNVDEAIAQLKRQRPVIKAVVLVATFR
AAAKFIEKTRDLYPDMIYATISGVGSTGLAGELMVLGPKYADGVICTQVTPAVDGYASVTLSYKKALAKYFPGEPADYTS
LEAYLTANILIEGLRRTGPALDTESLVTNLEAMQNYELGVGSKVSFGPSDHQALHKVWGTQLDKTGQYHAIDLD

Specific function: This Protein Is A Component Of The Leucine, Isoleucine, Valine, (Threonine) Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 43130; Mature: 42999

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKFDTRMANAAVAAQASAQPAVQGVTDTEIRFGMAAPFSGPAREMGRQLKLGIELAFAQ
CCCCHHHHHHHHHHHHCCCCCHHCCCCCCCEEEEECCCCCCHHHHHCHHHHHCEEEEEEE
VNEDGGVHGRQLKLTTADDAYEPTRTLNAMKDLYEQQKVFGFVENYGSPTALISVPYALE
ECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
RHALYFGAFTGAPSLRRDPPDRYVFNYRAGYAEEADAIVRYLVKNRRLRPQDIGVLTQQD
HCEEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHCCEEECCC
AYGDAGWDGVVKAMRGLRGGNAGEIFRMSYNRNTVNVDEAIAQLKRQRPVIKAVVLVATF
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHH
RAAAKFIEKTRDLYPDMIYATISGVGSTGLAGELMVLGPKYADGVICTQVTPAVDGYASV
HHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCHHHE
TLSYKKALAKYFPGEPADYTSLEAYLTANILIEGLRRTGPALDTESLVTNLEAMQNYELG
EHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
VGSKVSFGPSDHQALHKVWGTQLDKTGQYHAIDLD
CCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECC
>Mature Secondary Structure 
PKFDTRMANAAVAAQASAQPAVQGVTDTEIRFGMAAPFSGPAREMGRQLKLGIELAFAQ
CCCHHHHHHHHHHHHCCCCCHHCCCCCCCEEEEECCCCCCHHHHHCHHHHHCEEEEEEE
VNEDGGVHGRQLKLTTADDAYEPTRTLNAMKDLYEQQKVFGFVENYGSPTALISVPYALE
ECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
RHALYFGAFTGAPSLRRDPPDRYVFNYRAGYAEEADAIVRYLVKNRRLRPQDIGVLTQQD
HCEEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHCCEEECCC
AYGDAGWDGVVKAMRGLRGGNAGEIFRMSYNRNTVNVDEAIAQLKRQRPVIKAVVLVATF
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHH
RAAAKFIEKTRDLYPDMIYATISGVGSTGLAGELMVLGPKYADGVICTQVTPAVDGYASV
HHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCHHHE
TLSYKKALAKYFPGEPADYTSLEAYLTANILIEGLRRTGPALDTESLVTNLEAMQNYELG
EHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
VGSKVSFGPSDHQALHKVWGTQLDKTGQYHAIDLD
CCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA