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The map label for this gene is hisZ [H]

Identifier: 146342925

GI number: 146342925

Start: 6333014

End: 6334174

Strand: Direct

Name: hisZ [H]

Synonym: BRADO6106

Alternate gene names: 146342925

Gene position: 6333014-6334174 (Clockwise)

Preceding gene: 146342924

Following gene: 146342926

Centisome position: 84.93

GC content: 70.03

Gene sequence:

>1161_bases
ATGACCGGACCCGCTCCGACCGGACCCGCCGCGTGGGCGGACACGCTGCTGCAGTCCTTCGCCGAGGCCGGCTATGCCCG
CGCCGAGCCGGCGATCCTGCAGCCGGCCGAGCCGTTCCTCGACCTCTCCGGCGAGGACATTCGCAAGAGCCTGTATCTGA
CGACGGATGGCGGCGGCGAGGAGCTCTGCCTGCGCCCGGACCTGACCATTCCCGTCGCGCGCGACTATCTCGGCTCGGCT
CGCGCCGGCCAGCCCGCCGGATTCAGCTATCTCGGCCCGGTGTTTCGTTACCGCGGCGGCCGCCCGAGCGAGTTCCTGCA
GGGCGGCATCGAATCGTTCGGCCGCCAGGATCGTCCCGCCGCCGACGCCGAGATGCTGGCGCTCGCCATCGAGGCGACAG
CCGCGGCCGGCCTTGCCGAGGTCGAGATTCGCACCGGCGACGTCGCGCTGTTCACGGCCTTGATCGATGCGCTCGATCTT
TATCCGGTGTGGCGGCGCCGGCTGATCAAGGACTTCAACCGCCAGCGCACCTTGGCTCAGGATTTGGAGCGCCTGGCGAC
GGCCGAAGTGGCACCGCGCAACGAATATGAGGGCGTGCTCGCCGCGCTGGCCGGCTCAGACCGCAAGGCAGCGTTGGCGC
TGGTCACCGACCTGATGTCGATCGCCGGCGCCACCGCCGTGGGTGGCCGCACGGTCGCCGAGATCGCCGATCGATTCCTG
GAGCAATCCACCCTGAAAAGCGGCGCACTGCCGCGCGACGCGCTCGACCGCATCAAAAGATTTCTCGCCATCAGCGGCAG
CCCGGCTGACGCCGTCAAACAGCTCCGCGCGCTCGCCGCCGACGCCAAGCTCGACATCTCGGCTGCGATCGACGCCCTGG
ACGCGCGCACGGCCTTCATGGCCCAGCGCGGCATCGACGTCGCCAGAGTGCGCTTCGCCACCGCATTCGGCCGCGGCCTC
GACTACTACACCGGCTTCGAGTTCGAGCTGCACCACCCCGGCAATGGCGACGAGCCGCTGGTCGCCGGCGGCCGCTATGA
CGGCCTGCTGAACCAGCTCGGCGCCGCGTCCTTCATTCCGGCCGTCGGCTTCTCGATCTGGATCGAGCGGCTGGCGCAGA
GCAGCACGGCGGGCAAGGCCGCGCGCGGGAGAGCATCATGA

Upstream 100 bases:

>100_bases
CAGGAGCGACCATTCGGTGTCGAAACCGCGCAATGAGCATGCTAAGGGGCTGCCGCTGCCCGCCGGGCGGCGCGCATCCC
GATTTGGAACCAAGACCGAC

Downstream 100 bases:

>100_bases
GCGGCCCCCTCGTCCTCGCCGTTCCTTCCAAGGGTCGCCTGCAGGAGAACGCGGAGGCGTTCTTCGGCCGCGCCGGGCTG
AACCTGTCCAAGCCGGGCGG

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTGPAPTGPAAWADTLLQSFAEAGYARAEPAILQPAEPFLDLSGEDIRKSLYLTTDGGGEELCLRPDLTIPVARDYLGSA
RAGQPAGFSYLGPVFRYRGGRPSEFLQGGIESFGRQDRPAADAEMLALAIEATAAAGLAEVEIRTGDVALFTALIDALDL
YPVWRRRLIKDFNRQRTLAQDLERLATAEVAPRNEYEGVLAALAGSDRKAALALVTDLMSIAGATAVGGRTVAEIADRFL
EQSTLKSGALPRDALDRIKRFLAISGSPADAVKQLRALAADAKLDISAAIDALDARTAFMAQRGIDVARVRFATAFGRGL
DYYTGFEFELHHPGNGDEPLVAGGRYDGLLNQLGAASFIPAVGFSIWIERLAQSSTAGKAARGRAS

Sequences:

>Translated_386_residues
MTGPAPTGPAAWADTLLQSFAEAGYARAEPAILQPAEPFLDLSGEDIRKSLYLTTDGGGEELCLRPDLTIPVARDYLGSA
RAGQPAGFSYLGPVFRYRGGRPSEFLQGGIESFGRQDRPAADAEMLALAIEATAAAGLAEVEIRTGDVALFTALIDALDL
YPVWRRRLIKDFNRQRTLAQDLERLATAEVAPRNEYEGVLAALAGSDRKAALALVTDLMSIAGATAVGGRTVAEIADRFL
EQSTLKSGALPRDALDRIKRFLAISGSPADAVKQLRALAADAKLDISAAIDALDARTAFMAQRGIDVARVRFATAFGRGL
DYYTGFEFELHHPGNGDEPLVAGGRYDGLLNQLGAASFIPAVGFSIWIERLAQSSTAGKAARGRAS
>Mature_385_residues
TGPAPTGPAAWADTLLQSFAEAGYARAEPAILQPAEPFLDLSGEDIRKSLYLTTDGGGEELCLRPDLTIPVARDYLGSAR
AGQPAGFSYLGPVFRYRGGRPSEFLQGGIESFGRQDRPAADAEMLALAIEATAAAGLAEVEIRTGDVALFTALIDALDLY
PVWRRRLIKDFNRQRTLAQDLERLATAEVAPRNEYEGVLAALAGSDRKAALALVTDLMSIAGATAVGGRTVAEIADRFLE
QSTLKSGALPRDALDRIKRFLAISGSPADAVKQLRALAADAKLDISAAIDALDARTAFMAQRGIDVARVRFATAFGRGLD
YYTGFEFELHHPGNGDEPLVAGGRYDGLLNQLGAASFIPAVGFSIWIERLAQSSTAGKAARGRAS

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 41000; Mature: 40869

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPAPTGPAAWADTLLQSFAEAGYARAEPAILQPAEPFLDLSGEDIRKSLYLTTDGGGE
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCHHHCCEEEEEECCCCC
ELCLRPDLTIPVARDYLGSARAGQPAGFSYLGPVFRYRGGRPSEFLQGGIESFGRQDRPA
EEEECCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
ADAEMLALAIEATAAAGLAEVEIRTGDVALFTALIDALDLYPVWRRRLIKDFNRQRTLAQ
CCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLERLATAEVAPRNEYEGVLAALAGSDRKAALALVTDLMSIAGATAVGGRTVAEIADRFL
HHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHH
EQSTLKSGALPRDALDRIKRFLAISGSPADAVKQLRALAADAKLDISAAIDALDARTAFM
HHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AQRGIDVARVRFATAFGRGLDYYTGFEFELHHPGNGDEPLVAGGRYDGLLNQLGAASFIP
HHHCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEECCCHHHHHHHHCHHHHHH
AVGFSIWIERLAQSSTAGKAARGRAS
HHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TGPAPTGPAAWADTLLQSFAEAGYARAEPAILQPAEPFLDLSGEDIRKSLYLTTDGGGE
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCHHHCCEEEEEECCCCC
ELCLRPDLTIPVARDYLGSARAGQPAGFSYLGPVFRYRGGRPSEFLQGGIESFGRQDRPA
EEEECCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
ADAEMLALAIEATAAAGLAEVEIRTGDVALFTALIDALDLYPVWRRRLIKDFNRQRTLAQ
CCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLERLATAEVAPRNEYEGVLAALAGSDRKAALALVTDLMSIAGATAVGGRTVAEIADRFL
HHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHH
EQSTLKSGALPRDALDRIKRFLAISGSPADAVKQLRALAADAKLDISAAIDALDARTAFM
HHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AQRGIDVARVRFATAFGRGLDYYTGFEFELHHPGNGDEPLVAGGRYDGLLNQLGAASFIP
HHHCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEECCCHHHHHHHHCHHHHHH
AVGFSIWIERLAQSSTAGKAARGRAS
HHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA