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The map label for this gene is rsmE [H]

Identifier: 146342924

GI number: 146342924

Start: 6332143

End: 6332892

Strand: Direct

Name: rsmE [H]

Synonym: BRADO6105

Alternate gene names: 146342924

Gene position: 6332143-6332892 (Clockwise)

Preceding gene: 146342920

Following gene: 146342925

Centisome position: 84.92

GC content: 68.27

Gene sequence:

>750_bases
ATGCCCGATTACGACTTCACCGCCCCTCGCCTGTTCGTCGACGCTCCGCTGATCGAGGGCGGCGCGGTCGCGCTGGATCG
CAACCAGAGCAACTATCTCGGTAACGTGCTGCGTCTCGGCGCCGGCGATCAGGTGCTGGTTTTCAACGGCCGCGAGGGCG
AGTGGCAGGCCACGATCGCCGGCCGCAAGCGGGCGGACCTGCTGACCATCCAGCAGCGCCTCCGGCCGCAGGACCAGCTC
CCCGACATCACCTACGTCTTCGCCCCGCTGAAGCATGCGCGGCTCGACTACATCGTGCAGAAGGCGGTCGAGATGGGCGC
GGCCGTGTTGCAGCCGGTCCTCACCCGGCACACGCAGGTGTCGCGCCTGAACGGCGAGCGGATGCGCGCCAATGTGATCG
AGGCGGCTGAGCAATGCGGCATCCTCAGCCTGGCCGAGGTCAGGGATCCAGTGCCACTGGAGCGGTTCCTGCGCGAGCGC
ATGGCCTCACGTCTGCTGGTGTTCTGCGACGAGGCGGCCGAGATCGCCGATCCCGTTCAGGCGTTGCGCGGCGCGGCCGC
AAACGCCGGCATCGATCTCCTGATCGGCCCTGAAGGCGGCTTCGCCGCGGAGGAGCGTGAGCTGTTGCTGCGGCAAAGAC
AGGTGACCAGGTTGGCGCTCGGGCCGCGGATCCTGCGGGCTGATACCGCGGGGGTCGCGGCGCTCGCTTTGCTGCAGGCA
ACGCTCGGGGATTGGCGACAAAGCCGTTAA

Upstream 100 bases:

>100_bases
CAAAACGAGCCATCGCGCGCGCACCCTGCAAACCGTTCGTGGCTTTCCGGCCGGCACCCGGTCGGATAAGCAACATCCCT
GACCTCACAGCCCAATTGCC

Downstream 100 bases:

>100_bases
GGGCCGCGGGCGATTGTCGCGCAGGAGCGACCATTCGGTGTCGAAACCGCGCAATGAGCATGCTAAGGGGCTGCCGCTGC
CCGCCGGGCGGCGCGCATCC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MPDYDFTAPRLFVDAPLIEGGAVALDRNQSNYLGNVLRLGAGDQVLVFNGREGEWQATIAGRKRADLLTIQQRLRPQDQL
PDITYVFAPLKHARLDYIVQKAVEMGAAVLQPVLTRHTQVSRLNGERMRANVIEAAEQCGILSLAEVRDPVPLERFLRER
MASRLLVFCDEAAEIADPVQALRGAAANAGIDLLIGPEGGFAAEERELLLRQRQVTRLALGPRILRADTAGVAALALLQA
TLGDWRQSR

Sequences:

>Translated_249_residues
MPDYDFTAPRLFVDAPLIEGGAVALDRNQSNYLGNVLRLGAGDQVLVFNGREGEWQATIAGRKRADLLTIQQRLRPQDQL
PDITYVFAPLKHARLDYIVQKAVEMGAAVLQPVLTRHTQVSRLNGERMRANVIEAAEQCGILSLAEVRDPVPLERFLRER
MASRLLVFCDEAAEIADPVQALRGAAANAGIDLLIGPEGGFAAEERELLLRQRQVTRLALGPRILRADTAGVAALALLQA
TLGDWRQSR
>Mature_248_residues
PDYDFTAPRLFVDAPLIEGGAVALDRNQSNYLGNVLRLGAGDQVLVFNGREGEWQATIAGRKRADLLTIQQRLRPQDQLP
DITYVFAPLKHARLDYIVQKAVEMGAAVLQPVLTRHTQVSRLNGERMRANVIEAAEQCGILSLAEVRDPVPLERFLRERM
ASRLLVFCDEAAEIADPVQALRGAAANAGIDLLIGPEGGFAAEERELLLRQRQVTRLALGPRILRADTAGVAALALLQAT
LGDWRQSR

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=249, Percent_Identity=28.1124497991968, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27394; Mature: 27263

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDYDFTAPRLFVDAPLIEGGAVALDRNQSNYLGNVLRLGAGDQVLVFNGREGEWQATIA
CCCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHCCCCCCEEEEEECCCCCEEEEEC
GRKRADLLTIQQRLRPQDQLPDITYVFAPLKHARLDYIVQKAVEMGAAVLQPVLTRHTQV
CCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRLNGERMRANVIEAAEQCGILSLAEVRDPVPLERFLRERMASRLLVFCDEAAEIADPVQ
HHCCCHHHHHHHHHHHHHCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
ALRGAAANAGIDLLIGPEGGFAAEERELLLRQRQVTRLALGPRILRADTAGVAALALLQA
HHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHEECCHHHHHHHHHHHH
TLGDWRQSR
HHHHHHCCC
>Mature Secondary Structure 
PDYDFTAPRLFVDAPLIEGGAVALDRNQSNYLGNVLRLGAGDQVLVFNGREGEWQATIA
CCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHCCCCCCEEEEEECCCCCEEEEEC
GRKRADLLTIQQRLRPQDQLPDITYVFAPLKHARLDYIVQKAVEMGAAVLQPVLTRHTQV
CCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRLNGERMRANVIEAAEQCGILSLAEVRDPVPLERFLRERMASRLLVFCDEAAEIADPVQ
HHCCCHHHHHHHHHHHHHCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
ALRGAAANAGIDLLIGPEGGFAAEERELLLRQRQVTRLALGPRILRADTAGVAALALLQA
HHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHEECCHHHHHHHHHHHH
TLGDWRQSR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]