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The map label for this gene is ilvE [H]

Identifier: 146342859

GI number: 146342859

Start: 6263418

End: 6264497

Strand: Reverse

Name: ilvE [H]

Synonym: BRADO6039

Alternate gene names: 146342859

Gene position: 6264497-6263418 (Counterclockwise)

Preceding gene: 146342863

Following gene: 146342858

Centisome position: 84.01

GC content: 67.59

Gene sequence:

>1080_bases
ATGACTTTGACATTCGACATCAGGCCGACGGCGAACCCGACCTCCGACCAGGAGCGGGCGGCCAAGCTCGCCAATCCCGG
CTTCGGCCGCGTGTTCACCGATCACATGGCCGTGGTCCAGTACAGCCAGGACAAGGGCTGGCACAGCGCGCGGGTCGAAT
CCCGTGCCAACTTCCCGCTCGATCCGGCCGCGGCCGTGCTGCACTACGCGCAGGAGATCTTCGAGGGGCTGAAGGCCTAC
AAGCGCGACGATGGCGGCGTGAACCTGTTCCGCCCCGACGCCAACGCCAAGCGCTTCTGGAATTCGGCCGAGCGCATGGC
GATGGCGCAGCTGCCCGAGGCGGTCTTCATCGAGGCGGTCGAGCAGCTCGTCCGCATCGACCGCGCCTGGATCCCGGGCG
GCGAGGGCAGCCTGTATCTGCGCCCGTTCATGATCGCCAACGAGGTGTTCCTCGGCGTGAAGCCGTCGGCGGAGTACATT
TTCGCGGTCATCGCCTCGCCGGTCGGCTCCTATTTCAAGGGCGGCCCGGCCCCGGTCTCGATCTGGGTGTCGGAGAACTA
CACCCGGGCCGCGGTCGGCGGCACCGGCGGCGTCAAATGCGGCGGCAACTATGCCGCGAGCCTGAAGGCGCAGGCCGAGG
CGATGGCCCAGGGCTGCGATCAGGTCGTGTTCCTGGATGCGATCGAGCGGCGTTACGTCGAGGAGCTCGGCGGCATGAAC
GTGTTCTTCGTGTTCGCCGACGGCTCACTGTCGACGCCGCCGCTCGGCACCATCCTGCCGGGCATCACGCGAGACTCCAT
CATCAAGCTGGCGCGCGACGCCGGCCGCGAGGTGCGCGAGGAGGCCTATTCGATCGACCAGTGGCGCGCGGATGCCGCAA
GCGGCAAGCTCAAGGAAGCCTTCGCCTGCGGCACCGCCGCCGTGATCTCGCCGATCGGCAAGGTCCGCTCGGTGAGCGGC
GACGTCGTCATCAATGGCGGCGAGGCCGGCCCGGTGGCCATGGGCCTGCGCAAGCAGCTGGTCGACATCCAGTACGGCCG
AACTGCGGATCCGCATAATTGGATCAGGGACGTGAAGTAG

Upstream 100 bases:

>100_bases
CCGGTCACGGCAGGTCTGAAATTCAAAGGGCTGACCGACCCGGATCGACGGGGCGGCGCCAGAACTCGCGAACGGCGACC
AGAGCGGGGGAAGCAAGGAC

Downstream 100 bases:

>100_bases
GGGGCTGCTGCTGCTCCGTTCGCCGTCATGCCCGGGCTTGTCCCGGGCATCCGCGTCGTGCGGCATGAAAGTCGTGGATG
GCCGGGTCAAGCCCGGCCAT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTLTFDIRPTANPTSDQERAAKLANPGFGRVFTDHMAVVQYSQDKGWHSARVESRANFPLDPAAAVLHYAQEIFEGLKAY
KRDDGGVNLFRPDANAKRFWNSAERMAMAQLPEAVFIEAVEQLVRIDRAWIPGGEGSLYLRPFMIANEVFLGVKPSAEYI
FAVIASPVGSYFKGGPAPVSIWVSENYTRAAVGGTGGVKCGGNYAASLKAQAEAMAQGCDQVVFLDAIERRYVEELGGMN
VFFVFADGSLSTPPLGTILPGITRDSIIKLARDAGREVREEAYSIDQWRADAASGKLKEAFACGTAAVISPIGKVRSVSG
DVVINGGEAGPVAMGLRKQLVDIQYGRTADPHNWIRDVK

Sequences:

>Translated_359_residues
MTLTFDIRPTANPTSDQERAAKLANPGFGRVFTDHMAVVQYSQDKGWHSARVESRANFPLDPAAAVLHYAQEIFEGLKAY
KRDDGGVNLFRPDANAKRFWNSAERMAMAQLPEAVFIEAVEQLVRIDRAWIPGGEGSLYLRPFMIANEVFLGVKPSAEYI
FAVIASPVGSYFKGGPAPVSIWVSENYTRAAVGGTGGVKCGGNYAASLKAQAEAMAQGCDQVVFLDAIERRYVEELGGMN
VFFVFADGSLSTPPLGTILPGITRDSIIKLARDAGREVREEAYSIDQWRADAASGKLKEAFACGTAAVISPIGKVRSVSG
DVVINGGEAGPVAMGLRKQLVDIQYGRTADPHNWIRDVK
>Mature_358_residues
TLTFDIRPTANPTSDQERAAKLANPGFGRVFTDHMAVVQYSQDKGWHSARVESRANFPLDPAAAVLHYAQEIFEGLKAYK
RDDGGVNLFRPDANAKRFWNSAERMAMAQLPEAVFIEAVEQLVRIDRAWIPGGEGSLYLRPFMIANEVFLGVKPSAEYIF
AVIASPVGSYFKGGPAPVSIWVSENYTRAAVGGTGGVKCGGNYAASLKAQAEAMAQGCDQVVFLDAIERRYVEELGGMNV
FFVFADGSLSTPPLGTILPGITRDSIIKLARDAGREVREEAYSIDQWRADAASGKLKEAFACGTAAVISPIGKVRSVSGD
VVINGGEAGPVAMGLRKQLVDIQYGRTADPHNWIRDVK

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=332, Percent_Identity=40.6626506024096, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI38176287, Length=332, Percent_Identity=40.6626506024096, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI296010906, Length=332, Percent_Identity=40.6626506024096, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI50658084, Length=354, Percent_Identity=37.5706214689266, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI296010902, Length=332, Percent_Identity=36.4457831325301, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI296010900, Length=332, Percent_Identity=36.4457831325301, Blast_Score=198, Evalue=5e-51,
Organism=Homo sapiens, GI258614015, Length=293, Percent_Identity=37.2013651877133, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI48994963, Length=326, Percent_Identity=34.3558282208589, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17568601, Length=363, Percent_Identity=34.435261707989, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17565728, Length=349, Percent_Identity=34.0974212034384, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6322002, Length=366, Percent_Identity=37.9781420765027, Blast_Score=230, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6322608, Length=345, Percent_Identity=37.3913043478261, Blast_Score=222, Evalue=9e-59,
Organism=Drosophila melanogaster, GI24641779, Length=335, Percent_Identity=40.5970149253731, Blast_Score=236, Evalue=1e-62,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 38852; Mature: 38721

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTFDIRPTANPTSDQERAAKLANPGFGRVFTDHMAVVQYSQDKGWHSARVESRANFPL
CEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHEEEEECCCCCCCHHHHCCCCCCCC
DPAAAVLHYAQEIFEGLKAYKRDDGGVNLFRPDANAKRFWNSAERMAMAQLPEAVFIEAV
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
EQLVRIDRAWIPGGEGSLYLRPFMIANEVFLGVKPSAEYIFAVIASPVGSYFKGGPAPVS
HHHHHHHHHCCCCCCCCEEEEEEEEECEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEE
IWVSENYTRAAVGGTGGVKCGGNYAASLKAQAEAMAQGCDQVVFLDAIERRYVEELGGMN
EEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCE
VFFVFADGSLSTPPLGTILPGITRDSIIKLARDAGREVREEAYSIDQWRADAASGKLKEA
EEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FACGTAAVISPIGKVRSVSGDVVINGGEAGPVAMGLRKQLVDIQYGRTADPHNWIRDVK
HHCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
TLTFDIRPTANPTSDQERAAKLANPGFGRVFTDHMAVVQYSQDKGWHSARVESRANFPL
EEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHEEEEECCCCCCCHHHHCCCCCCCC
DPAAAVLHYAQEIFEGLKAYKRDDGGVNLFRPDANAKRFWNSAERMAMAQLPEAVFIEAV
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
EQLVRIDRAWIPGGEGSLYLRPFMIANEVFLGVKPSAEYIFAVIASPVGSYFKGGPAPVS
HHHHHHHHHCCCCCCCCEEEEEEEEECEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEE
IWVSENYTRAAVGGTGGVKCGGNYAASLKAQAEAMAQGCDQVVFLDAIERRYVEELGGMN
EEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCE
VFFVFADGSLSTPPLGTILPGITRDSIIKLARDAGREVREEAYSIDQWRADAASGKLKEA
EEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FACGTAAVISPIGKVRSVSGDVVINGGEAGPVAMGLRKQLVDIQYGRTADPHNWIRDVK
HHCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]