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The map label for this gene is hisS [H]

Identifier: 146342858

GI number: 146342858

Start: 6261491

End: 6263131

Strand: Reverse

Name: hisS [H]

Synonym: BRADO6038

Alternate gene names: 146342858

Gene position: 6263131-6261491 (Counterclockwise)

Preceding gene: 146342859

Following gene: 146342857

Centisome position: 83.99

GC content: 62.77

Gene sequence:

>1641_bases
ATGAACGACAAACCCAAGAAACCCCAGAAGCTGAAGGCGCGGCTGCCGCGCGGGCTCGAGGATCGCGGCCCGGCGGCGAT
CGCCGCCACCCGCGAAATGGTCGAGAAGATCCGCGCCGTCTACGAGCGCTACGGCTTCGAGCCGGTGGAGACGCCGGCAT
TCGAATACACCGATGCGCTCGGCAAATTCCTGCCCGACCAGGACCGCCCGAACGAGGGCGTGTTCTCGTTCCAGGACGAT
GACGAGCAGTGGATCTCGCTGCGCTACGACCTGACCGCGCCGCTGGCGCGCTACGTCGCCGAGAATTTCGACGCGCTGCC
GAAGCCGTATCGTTCTTATCGAGCAGGCTACGTGTTCCGCAACGAGAAGCCCGGTCCCGGCCGCTTCCGCCAGTTCATGC
AGTTCGACGCCGACACCGTCGGCTCCGGTTCGCCGGCGGCGGATGCCGAAATGTGCATGATGGCGGCGGACACGATGGAG
GCGCTCGGCATTGCCCGCGGCGACTACGCGGTCAAGGTCAATAGTCGAAAATTGCTCGATGGCGCCTTAGAGAGCATTGG
TCTTGGTGGCGATGACAATGCTGGCAGGCGACTCGCCGTTCTGCGAGCGCTCGACAAGTATGATCGTCTTGGTGACCGCG
GCGTGCGTGAACTGCTGGGGCCAGGACGGAAGGATGAAAGCGGTGACTTTACCAAAGGAGCCGGGCTCATCGAGGATGAT
ATTACCCAAATAATGGGACTTTTCGAGCTTACGCCGCAATCGGCAAGTGATTTTGACCATCTGTCGGAATGGGAAAAACA
AGAATCCAGCTACGTCGAAGTCAATTTCGATCAACGCCGTGGGCATCTGGTGAGGGAGATTCATTCGGATTTTGCGCCGG
GGCGACTTCCGATCAATAATTTGGCTACGATTAAACTCCTTCGCAGCTGGTTCGGAGATTCTGCCAAAGTCTCGGAAGGC
TTGGATGAGCTAGAACAAATTGCTCAGCTCGGGCAGGCTGCGCGCTATGGCTCCAAGCGGATCCGCATCGATCCATCGGT
CGTTCGTGGTCTTGAATACTACACCGGTCCGGTCTTCGAGGTTGAGCTTCTTACCGCGATTCCGAACGAGAAGGGCCAGC
CGGTGGTGTTCGGCTCGGTCGGCGGCGGCGGCCGCTACGATGGTCTCGTCTCGCGCTTCCGCGGCGAGCCGGTGCCGGCG
ACCGGGTTCTCAATCGGTGTCTCCCGTCTGCAGGCGGCGCTGACCCTGCTCGGCAAGCTCGACACGCGGCCGGCCTTCGG
CCCCGTTGTCGTCACCGTGTTCGACCGCGACCGCGTCGCCGACTATCAGAAGATGGTCTCGACCCTGCGCGGCCACGGCA
TCCGCGCCGAGCTCTATCTCGGCAACCCGAAGAACATGGGCAACCAGCTCAAATATGCCGACCGCCGCAACGCGCCCTGC
GTCATCATCCAGGGCTCGGACGAGAAGGCGCGCGGCGAGGTGCAGATCAAGGACCTGATCGAGGGCGCCAAGGCCGCCGC
CGCGATCGCCTCCAACCAGGAATGGCGCGAGAGCCGCCCGGCGCAGGTCTCCGCCAAGGAGGACGAACTCGTCGCCGCCG
TCCGCGAGGTGCTCGCGCGGCACGATGTGAGCTGGGGGTAA

Upstream 100 bases:

>100_bases
GGTGGGCAAAGGACCGCGAAGCGGGCCGTGCCCACCGTTCCGGTATTTGTCAGCCATGGTGGGCACGCTGCGCTTTGCCC
ACCCTACGCTCTGGTATCAA

Downstream 100 bases:

>100_bases
CGAGACCAGGCAATCATCCGGCTCGGTTATCTTGCCGTCATTGCGAGCGAAGCGAAGCAATCCAGGGGCCAAACAGAACG
ATCTTTCGGAGTAACCCTGG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MNDKPKKPQKLKARLPRGLEDRGPAAIAATREMVEKIRAVYERYGFEPVETPAFEYTDALGKFLPDQDRPNEGVFSFQDD
DEQWISLRYDLTAPLARYVAENFDALPKPYRSYRAGYVFRNEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTME
ALGIARGDYAVKVNSRKLLDGALESIGLGGDDNAGRRLAVLRALDKYDRLGDRGVRELLGPGRKDESGDFTKGAGLIEDD
ITQIMGLFELTPQSASDFDHLSEWEKQESSYVEVNFDQRRGHLVREIHSDFAPGRLPINNLATIKLLRSWFGDSAKVSEG
LDELEQIAQLGQAARYGSKRIRIDPSVVRGLEYYTGPVFEVELLTAIPNEKGQPVVFGSVGGGGRYDGLVSRFRGEPVPA
TGFSIGVSRLQAALTLLGKLDTRPAFGPVVVTVFDRDRVADYQKMVSTLRGHGIRAELYLGNPKNMGNQLKYADRRNAPC
VIIQGSDEKARGEVQIKDLIEGAKAAAAIASNQEWRESRPAQVSAKEDELVAAVREVLARHDVSWG

Sequences:

>Translated_546_residues
MNDKPKKPQKLKARLPRGLEDRGPAAIAATREMVEKIRAVYERYGFEPVETPAFEYTDALGKFLPDQDRPNEGVFSFQDD
DEQWISLRYDLTAPLARYVAENFDALPKPYRSYRAGYVFRNEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTME
ALGIARGDYAVKVNSRKLLDGALESIGLGGDDNAGRRLAVLRALDKYDRLGDRGVRELLGPGRKDESGDFTKGAGLIEDD
ITQIMGLFELTPQSASDFDHLSEWEKQESSYVEVNFDQRRGHLVREIHSDFAPGRLPINNLATIKLLRSWFGDSAKVSEG
LDELEQIAQLGQAARYGSKRIRIDPSVVRGLEYYTGPVFEVELLTAIPNEKGQPVVFGSVGGGGRYDGLVSRFRGEPVPA
TGFSIGVSRLQAALTLLGKLDTRPAFGPVVVTVFDRDRVADYQKMVSTLRGHGIRAELYLGNPKNMGNQLKYADRRNAPC
VIIQGSDEKARGEVQIKDLIEGAKAAAAIASNQEWRESRPAQVSAKEDELVAAVREVLARHDVSWG
>Mature_546_residues
MNDKPKKPQKLKARLPRGLEDRGPAAIAATREMVEKIRAVYERYGFEPVETPAFEYTDALGKFLPDQDRPNEGVFSFQDD
DEQWISLRYDLTAPLARYVAENFDALPKPYRSYRAGYVFRNEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTME
ALGIARGDYAVKVNSRKLLDGALESIGLGGDDNAGRRLAVLRALDKYDRLGDRGVRELLGPGRKDESGDFTKGAGLIEDD
ITQIMGLFELTPQSASDFDHLSEWEKQESSYVEVNFDQRRGHLVREIHSDFAPGRLPINNLATIKLLRSWFGDSAKVSEG
LDELEQIAQLGQAARYGSKRIRIDPSVVRGLEYYTGPVFEVELLTAIPNEKGQPVVFGSVGGGGRYDGLVSRFRGEPVPA
TGFSIGVSRLQAALTLLGKLDTRPAFGPVVVTVFDRDRVADYQKMVSTLRGHGIRAELYLGNPKNMGNQLKYADRRNAPC
VIIQGSDEKARGEVQIKDLIEGAKAAAAIASNQEWRESRPAQVSAKEDELVAAVREVLARHDVSWG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=507, Percent_Identity=29.9802761341223, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI15029520, Length=543, Percent_Identity=27.8084714548803, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1788861, Length=201, Percent_Identity=27.8606965174129, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71993686, Length=557, Percent_Identity=28.9048473967684, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71993693, Length=557, Percent_Identity=28.9048473967684, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6325290, Length=527, Percent_Identity=25.6166982922201, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24643061, Length=205, Percent_Identity=33.6585365853659, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24643059, Length=224, Percent_Identity=30.3571428571429, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 60369; Mature: 60369

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKPKKPQKLKARLPRGLEDRGPAAIAATREMVEKIRAVYERYGFEPVETPAFEYTDAL
CCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
GKFLPDQDRPNEGVFSFQDDDEQWISLRYDLTAPLARYVAENFDALPKPYRSYRAGYVFR
HHHCCCCCCCCCCCEECCCCCCEEEEEEECHHHHHHHHHHHHHCCCCCCHHHHCCCEEEE
NEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTMEALGIARGDYAVKVNSRKLLD
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHH
GALESIGLGGDDNAGRRLAVLRALDKYDRLGDRGVRELLGPGRKDESGDFTKGAGLIEDD
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
ITQIMGLFELTPQSASDFDHLSEWEKQESSYVEVNFDQRRGHLVREIHSDFAPGRLPINN
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCH
LATIKLLRSWFGDSAKVSEGLDELEQIAQLGQAARYGSKRIRIDPSVVRGLEYYTGPVFE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCEEE
VELLTAIPNEKGQPVVFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRLQAALTLLGKL
EEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
DTRPAFGPVVVTVFDRDRVADYQKMVSTLRGHGIRAELYLGNPKNMGNQLKYADRRNAPC
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHCCCCCE
VIIQGSDEKARGEVQIKDLIEGAKAAAAIASNQEWRESRPAQVSAKEDELVAAVREVLAR
EEEECCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHH
HDVSWG
CCCCCC
>Mature Secondary Structure
MNDKPKKPQKLKARLPRGLEDRGPAAIAATREMVEKIRAVYERYGFEPVETPAFEYTDAL
CCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
GKFLPDQDRPNEGVFSFQDDDEQWISLRYDLTAPLARYVAENFDALPKPYRSYRAGYVFR
HHHCCCCCCCCCCCEECCCCCCEEEEEEECHHHHHHHHHHHHHCCCCCCHHHHCCCEEEE
NEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTMEALGIARGDYAVKVNSRKLLD
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHH
GALESIGLGGDDNAGRRLAVLRALDKYDRLGDRGVRELLGPGRKDESGDFTKGAGLIEDD
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
ITQIMGLFELTPQSASDFDHLSEWEKQESSYVEVNFDQRRGHLVREIHSDFAPGRLPINN
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCH
LATIKLLRSWFGDSAKVSEGLDELEQIAQLGQAARYGSKRIRIDPSVVRGLEYYTGPVFE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCEEE
VELLTAIPNEKGQPVVFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRLQAALTLLGKL
EEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
DTRPAFGPVVVTVFDRDRVADYQKMVSTLRGHGIRAELYLGNPKNMGNQLKYADRRNAPC
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHCCCCCE
VIIQGSDEKARGEVQIKDLIEGAKAAAAIASNQEWRESRPAQVSAKEDELVAAVREVLAR
EEEECCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHH
HDVSWG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]