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The map label for this gene is ligE [H]

Identifier: 146342812

GI number: 146342812

Start: 6218942

End: 6219631

Strand: Reverse

Name: ligE [H]

Synonym: BRADO5989

Alternate gene names: 146342812

Gene position: 6219631-6218942 (Counterclockwise)

Preceding gene: 146342813

Following gene: 146342810

Centisome position: 83.41

GC content: 67.97

Gene sequence:

>690_bases
TTGAAGCTGTTCGAACTCGTCGGGCGTGACGCGGGGCGTCCGTTCAGCCCATTTTGCTGGCGCACGCGGATGGCGCTGGC
GCACAAGGGCCTGAGCGCCGAGTCCATCCCCTGGCGCTTCACCGAGAAGGGAGCGATCGCGGCGCACGGCTCGGAGAAGG
TCCCGGTCATGCTCGACGGCGACCGCGCCGTAGCGGACTCCTGGGCCATCGCGAACTATTTGGAAGACAATCATCCGGAC
CGGCCGTCGCTGTTCGGCGGCGCCGGCGGCCGTGCGATGGCGCGGTTCATGAACGCCTGGGGCGACATCGCCATCGTCGG
CGGCATCTTCCCGCTGATCATCGCCGATATCCCGAACAATCTGGCGGCGGAAGACGCCGCCTATTTCCGTGCGTCGCGCG
AGGCGCGGTTCGGCAAGAGCCTCGAGGACATCATGGCGACGCGCGACCAGGCCGTGACCGGCTTCCGCCGCTCGCTCGAG
CCGCTGCGCCAGACCTTGAAGACGCAACCCTTCATCGGCGGCGACGCGCCCAATTATGCCGACTACATCGTGTTCGGCGG
CTTCCAATGGGCGCGGGTGGTCAGCCCGTTCCGCCTGCTGGAGGGGAGCGACAGCGTCTACGCCTGGCGCGAGCGGCTGC
TCGATGCTTTCGATGGGCTGGCGCGCAAGTCGCCGGGCTTCGCGCTCTAG

Upstream 100 bases:

>100_bases
GCATCGTCGTCACCGAGGCCGGCGCGCGACTGCTGCGCGACAACGGCCGTGTCCTGGGCTAGAACCGGTCCGTCAAGGGG
AGGATGGACCGGGCATGGCG

Downstream 100 bases:

>100_bases
GCGGCAGGCGACCGGCGCGACCGCCACGCTGTCAGGCCATCTTCCAGATCGCGGCTGCCGACCAGCCGCGCGAATTGTTC
GCTCTCGACCTGCAGCCCTT

Product: putative glutathione S-transferase (GST)

Products: NA

Alternate protein names: Beta-aryl ether cleaving enzyme [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKLFELVGRDAGRPFSPFCWRTRMALAHKGLSAESIPWRFTEKGAIAAHGSEKVPVMLDGDRAVADSWAIANYLEDNHPD
RPSLFGGAGGRAMARFMNAWGDIAIVGGIFPLIIADIPNNLAAEDAAYFRASREARFGKSLEDIMATRDQAVTGFRRSLE
PLRQTLKTQPFIGGDAPNYADYIVFGGFQWARVVSPFRLLEGSDSVYAWRERLLDAFDGLARKSPGFAL

Sequences:

>Translated_229_residues
MKLFELVGRDAGRPFSPFCWRTRMALAHKGLSAESIPWRFTEKGAIAAHGSEKVPVMLDGDRAVADSWAIANYLEDNHPD
RPSLFGGAGGRAMARFMNAWGDIAIVGGIFPLIIADIPNNLAAEDAAYFRASREARFGKSLEDIMATRDQAVTGFRRSLE
PLRQTLKTQPFIGGDAPNYADYIVFGGFQWARVVSPFRLLEGSDSVYAWRERLLDAFDGLARKSPGFAL
>Mature_229_residues
MKLFELVGRDAGRPFSPFCWRTRMALAHKGLSAESIPWRFTEKGAIAAHGSEKVPVMLDGDRAVADSWAIANYLEDNHPD
RPSLFGGAGGRAMARFMNAWGDIAIVGGIFPLIIADIPNNLAAEDAAYFRASREARFGKSLEDIMATRDQAVTGFRRSLE
PLRQTLKTQPFIGGDAPNYADYIVFGGFQWARVVSPFRLLEGSDSVYAWRERLLDAFDGLARKSPGFAL

Specific function: Able to degrade various dimeric lignin compounds. Catalyzes the unique and reductive cleavage of arylglycerol-beta- aryl ether [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 25274; Mature: 25274

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFELVGRDAGRPFSPFCWRTRMALAHKGLSAESIPWRFTEKGAIAAHGSEKVPVMLDG
CCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEECCCCCCCEEECC
DRAVADSWAIANYLEDNHPDRPSLFGGAGGRAMARFMNAWGDIAIVGGIFPLIIADIPNN
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
LAAEDAAYFRASREARFGKSLEDIMATRDQAVTGFRRSLEPLRQTLKTQPFIGGDAPNYA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DYIVFGGFQWARVVSPFRLLEGSDSVYAWRERLLDAFDGLARKSPGFAL
CEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLFELVGRDAGRPFSPFCWRTRMALAHKGLSAESIPWRFTEKGAIAAHGSEKVPVMLDG
CCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEECCCCCCCEEECC
DRAVADSWAIANYLEDNHPDRPSLFGGAGGRAMARFMNAWGDIAIVGGIFPLIIADIPNN
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
LAAEDAAYFRASREARFGKSLEDIMATRDQAVTGFRRSLEPLRQTLKTQPFIGGDAPNYA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DYIVFGGFQWARVVSPFRLLEGSDSVYAWRERLLDAFDGLARKSPGFAL
CEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1744051; 8495726 [H]