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The map label for this gene is napB [H]

Identifier: 146342745

GI number: 146342745

Start: 6136514

End: 6136966

Strand: Direct

Name: napB [H]

Synonym: BRADO5917

Alternate gene names: 146342745

Gene position: 6136514-6136966 (Clockwise)

Preceding gene: 146342744

Following gene: 146342746

Centisome position: 82.3

GC content: 65.56

Gene sequence:

>453_bases
ATGATGCTGCTCGCGGCCAGCCTGGCGGCCGGCTCGAGCGCCCTGCTCGCCCAGAGCGTCTCGTCGTCGCTGCGCGGCCC
GACACCGCTCAATGACGAAGGCCCGGCACCGCCGATGCAGCCGACCAAGAACACCGCAGAGAGAGAGGTGCGCAACTATC
CGGAGCAGCCGCCGGTGATCCCGCACTCCATCGACGGCTATCAGGTTGACATGCAGGGCAACAAATGCCTGTCCTGCCAT
GCGCGTGCGCGCACTGCGGAATCGAAGGCACCGATGCTGTCGATCACCCACTTCATGGACCGTGACGGCCAGTTCCTGGC
CTCGGTGTCGCCGCGGCGCTACTTCTGCACGCAGTGCCATGTGTCGCAAAGTGTCGCCACCCCGCCGGTCAGCAACGATT
TCGTCGACATCGACACGGTGCTGTCGCACGACAAGCCGGGTGCCAAGCGATGA

Upstream 100 bases:

>100_bases
TGACGCTCGACGCCACCGATCCGATCTCGCTGCAGACCGACTACAAGAAATGCGCCGTGCGCATCGAGCGGGTGTGACCG
ACATGATCAAGAAACTTGCG

Downstream 100 bases:

>100_bases
GCAGCGCCGACATCCCGCGGGGGACGGGAGCGCCGGTCCCCGAGAAACGCCTCGGCCTCGTCAGACGCACCTGGCAATTC
GTGCTCGATCTGATCGACGT

Product: periplasmic nitrate reductase small subunit diheme cytochrome c

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MMLLAASLAAGSSALLAQSVSSSLRGPTPLNDEGPAPPMQPTKNTAEREVRNYPEQPPVIPHSIDGYQVDMQGNKCLSCH
ARARTAESKAPMLSITHFMDRDGQFLASVSPRRYFCTQCHVSQSVATPPVSNDFVDIDTVLSHDKPGAKR

Sequences:

>Translated_150_residues
MMLLAASLAAGSSALLAQSVSSSLRGPTPLNDEGPAPPMQPTKNTAEREVRNYPEQPPVIPHSIDGYQVDMQGNKCLSCH
ARARTAESKAPMLSITHFMDRDGQFLASVSPRRYFCTQCHVSQSVATPPVSNDFVDIDTVLSHDKPGAKR
>Mature_150_residues
MMLLAASLAAGSSALLAQSVSSSLRGPTPLNDEGPAPPMQPTKNTAEREVRNYPEQPPVIPHSIDGYQVDMQGNKCLSCH
ARARTAESKAPMLSITHFMDRDGQFLASVSPRRYFCTQCHVSQSVATPPVSNDFVDIDTVLSHDKPGAKR

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=129, Percent_Identity=42.6356589147287, Blast_Score=102, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 16205; Mature: 16205

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLLAASLAAGSSALLAQSVSSSLRGPTPLNDEGPAPPMQPTKNTAEREVRNYPEQPPVI
CEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
PHSIDGYQVDMQGNKCLSCHARARTAESKAPMLSITHFMDRDGQFLASVSPRRYFCTQCH
CCCCCCEEEEECCCEEEEHHHHHHCCCCCCCEEEHHHHHCCCCCEEECCCCCCEEHHHHH
VSQSVATPPVSNDFVDIDTVLSHDKPGAKR
CCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MMLLAASLAAGSSALLAQSVSSSLRGPTPLNDEGPAPPMQPTKNTAEREVRNYPEQPPVI
CEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
PHSIDGYQVDMQGNKCLSCHARARTAESKAPMLSITHFMDRDGQFLASVSPRRYFCTQCH
CCCCCCEEEEECCCEEEEHHHHHHCCCCCCCEEEHHHHHCCCCCEEECCCCCCEEHHHHH
VSQSVATPPVSNDFVDIDTVLSHDKPGAKR
CCCCCCCCCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]