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The map label for this gene is napC [H]

Identifier: 146342746

GI number: 146342746

Start: 6136963

End: 6137625

Strand: Direct

Name: napC [H]

Synonym: BRADO5918

Alternate gene names: 146342746

Gene position: 6136963-6137625 (Clockwise)

Preceding gene: 146342745

Following gene: 146342747

Centisome position: 82.3

GC content: 64.86

Gene sequence:

>663_bases
ATGAGCAGCGCCGACATCCCGCGGGGGACGGGAGCGCCGGTCCCCGAGAAACGCCTCGGCCTCGTCAGACGCACCTGGCA
ATTCGTGCTCGATCTGATCGACGTGCTGCTCAAGCCGAGCTCGATCTTCGGGCTCGGCGTGCTGGTGCTCGCCGGCTTCG
TCGCCGGTGTGATCTTCTGGGGCGGCTTCAACACCGCGCTGGAGATCACCAACACCGAGAAATTCTGCACCGGCTGTCAC
GAGATGAAGGACAACGTGTTCGCCGAGTTGAAGACCACCGTGCACTTCTCCAATCGCTCCGGCGTCCGCGCCTCCTGCCC
GGACTGCCACGTGCCGCACAATTGGACCGACAAGATCGCGCGCAAGATGCAGGCGTCGAAGGAGGTCTGGGGCCACCTGT
TCGGCTCGATCGACACCCGCGAGAAGTTCACTGGTCGACGGCTGGAGCTGGCACTGCACGAATGGGCACGCTTCAAGGCG
AACGACTCGCTGGAATGCCGCAACTGCCACAGCGCGCAGTCGATGGACATCACCAAGCAGTCGCCCCGCGCCGTCGAAGC
CCACCAGCGCTTCCTCTTCACCGGCGAGAAGACCTGCATCGACTGCCACAAGGGCATCGCGCACCACCTTCCGGACATGC
GCGGCGTCCCGGGCTGGCAGTAG

Upstream 100 bases:

>100_bases
CGCAGTGCCATGTGTCGCAAAGTGTCGCCACCCCGCCGGTCAGCAACGATTTCGTCGACATCGACACGGTGCTGTCGCAC
GACAAGCCGGGTGCCAAGCG

Downstream 100 bases:

>100_bases
GTTCTTCTGAGTTGGCGAACTTTCGCCACACGCGAGGTCAACTCCCCTCCCCCTTGCGGGGAGGGGTTGGGGGTGGGGGT
CCACACGGGCAGTCTTTGCT

Product: periplasmic nitrate reductase, four-heme c-type cytochrome (NapC/NirT)

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSSADIPRGTGAPVPEKRLGLVRRTWQFVLDLIDVLLKPSSIFGLGVLVLAGFVAGVIFWGGFNTALEITNTEKFCTGCH
EMKDNVFAELKTTVHFSNRSGVRASCPDCHVPHNWTDKIARKMQASKEVWGHLFGSIDTREKFTGRRLELALHEWARFKA
NDSLECRNCHSAQSMDITKQSPRAVEAHQRFLFTGEKTCIDCHKGIAHHLPDMRGVPGWQ

Sequences:

>Translated_220_residues
MSSADIPRGTGAPVPEKRLGLVRRTWQFVLDLIDVLLKPSSIFGLGVLVLAGFVAGVIFWGGFNTALEITNTEKFCTGCH
EMKDNVFAELKTTVHFSNRSGVRASCPDCHVPHNWTDKIARKMQASKEVWGHLFGSIDTREKFTGRRLELALHEWARFKA
NDSLECRNCHSAQSMDITKQSPRAVEAHQRFLFTGEKTCIDCHKGIAHHLPDMRGVPGWQ
>Mature_219_residues
SSADIPRGTGAPVPEKRLGLVRRTWQFVLDLIDVLLKPSSIFGLGVLVLAGFVAGVIFWGGFNTALEITNTEKFCTGCHE
MKDNVFAELKTTVHFSNRSGVRASCPDCHVPHNWTDKIARKMQASKEVWGHLFGSIDTREKFTGRRLELALHEWARFKAN
DSLECRNCHSAQSMDITKQSPRAVEAHQRFLFTGEKTCIDCHKGIAHHLPDMRGVPGWQ

Specific function: Mediates electron flow from quinones to the NapAB complex [H]

COG id: COG3005

COG function: function code C; Nitrate/TMAO reductases, membrane-bound tetraheme cytochrome c subunit

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788530, Length=205, Percent_Identity=51.219512195122, Blast_Score=225, Evalue=2e-60,
Organism=Escherichia coli, GI1787230, Length=183, Percent_Identity=43.1693989071038, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1788181, Length=150, Percent_Identity=45.3333333333333, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005126
- InterPro:   IPR011885 [H]

Pfam domain/function: PF03264 Cytochrom_NNT [H]

EC number: NA

Molecular weight: Translated: 24672; Mature: 24540

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSADIPRGTGAPVPEKRLGLVRRTWQFVLDLIDVLLKPSSIFGLGVLVLAGFVAGVIFW
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GGFNTALEITNTEKFCTGCHEMKDNVFAELKTTVHFSNRSGVRASCPDCHVPHNWTDKIA
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCHHHHHH
RKMQASKEVWGHLFGSIDTREKFTGRRLELALHEWARFKANDSLECRNCHSAQSMDITKQ
HHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
SPRAVEAHQRFLFTGEKTCIDCHKGIAHHLPDMRGVPGWQ
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SSADIPRGTGAPVPEKRLGLVRRTWQFVLDLIDVLLKPSSIFGLGVLVLAGFVAGVIFW
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GGFNTALEITNTEKFCTGCHEMKDNVFAELKTTVHFSNRSGVRASCPDCHVPHNWTDKIA
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCHHHHHH
RKMQASKEVWGHLFGSIDTREKFTGRRLELALHEWARFKANDSLECRNCHSAQSMDITKQ
HHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
SPRAVEAHQRFLFTGEKTCIDCHKGIAHHLPDMRGVPGWQ
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7639719 [H]