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The map label for this gene is amiC [H]

Identifier: 146342707

GI number: 146342707

Start: 6092814

End: 6094070

Strand: Reverse

Name: amiC [H]

Synonym: BRADO5874

Alternate gene names: 146342707

Gene position: 6094070-6092814 (Counterclockwise)

Preceding gene: 146342708

Following gene: 146342706

Centisome position: 81.73

GC content: 60.7

Gene sequence:

>1257_bases
ATGGCAGTCGATCGACGCAAATTCTTGAAGAGCGGTGCCCTGGCGGGCGGCGCGCTGATTACCTCGCCATGGGTGTTCTC
GACCGGCGCGCAGGCGGCCGGCGAGATCAAGGTGGGCGTGCTGTTCTCGCTTACCGGCGGTCTTTCGATCGTCGAGAAGT
CGCTGCACGATGCGACGATGATGGCGATCAGCGAGATCAACGCCGCCGGTGGCGTCAAGGGGATGAAAATCTCTGCGATC
GCCGAGGATGGCGCATCCGATCCCAAGACGTATAACGAGAAGGCGTCGAAGCTCGTCATCCAGGACAAGGTCCCGACCGT
GTTCGGCTCCTATACGTCCGCCAGCCGCAAGGCGGTCCTGCCGGTGTTCGAGAAGCGCAACAATCTCTATTTCTATCCGA
CCTATTATGAAGGCTTCGAGTGCTCGAAGAACGTCGTCTATACCGGCGCCGTTCCGAACCAGCAGCTGTCGAACTTCATT
CCCTGGATCATCAAGACGCTCGGCAAGAAGAAGTTCTTCATCGTCGGGTCGAACTACATCTATCCGCGCGAGATGGCGAA
GGTCTCCAAGATCCTGATCGAGAAGAACGGCGGCGAGTGGATCGCCGACGAATATCTCGAGCTCGGTCATTCTGAATGGG
GCTCGATGGTCAACAAGATCAAGAGCTCCGGCTGCGACGTCGTGCTGTCCAACGTGGTCGGCGACTCCGTGATCGCGTTC
TATCGCGAGTACAAGAACCAGGGCCTGACCCACGACAAGCTGCCGATCTGCGCGACGGTCACCTCTGAGATCGAGATCGC
GGCGATGGGCGCCGAATACGCCGTCGGCTCCTTCACCTCGTTCCCGTACTTCCAGGCGATCGACACCGACCGCAACAAGG
CGTTCATCGAGCGCTACCGTAAGTTCGTCAAGGACCCCAAGGCGGTGACGCACCACGCGCTTGAATCGTCGTACTTCCAG
GTCTTCCTGTGGAAGCAGGCGGTGGAGAAGGCCGCCGAGATCACGCCGGTGGCGATCCGCGAGGCGGTGAAGGGCCAGGA
GTTCGACGCGCCCAACGGCCACGTCAAGATCGAGGCCGAGAACCTGCATACGTACCTGACGCCGCGCATCGCGCAGTGGC
TGCCGGACGGGCAGGGCAAGATCATCGATGCGTACAAGGAGCCGGTCATGCCGCTTCCTTACGTCGCCTACGGCGAAACT
CCGACGAACCTCTTCTGCACCGGCAAGGGGCTCGACGCGGCCAAGCTGAAAACCTGA

Upstream 100 bases:

>100_bases
CGTCGTCGGAGCTCGATCCGCTCTGGGCGATTTTTGGCAACAAGCAGCGTGCTTCGGCGCGAACGAGGACGCGTGCCTGG
ACAAACGGGGAGTGTGCGGT

Downstream 100 bases:

>100_bases
CGTCAAGGAAACCCTGTCCACCGCGCGCCTCCCTCGCGCGGTGGATGGCGGGAGGCATCTGTCATGCTCGAGGTTCTGTT
TATCGGTCTCAGCCTGGGAT

Product: putative aliphatic amidase expression-regulating protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MAVDRRKFLKSGALAGGALITSPWVFSTGAQAAGEIKVGVLFSLTGGLSIVEKSLHDATMMAISEINAAGGVKGMKISAI
AEDGASDPKTYNEKASKLVIQDKVPTVFGSYTSASRKAVLPVFEKRNNLYFYPTYYEGFECSKNVVYTGAVPNQQLSNFI
PWIIKTLGKKKFFIVGSNYIYPREMAKVSKILIEKNGGEWIADEYLELGHSEWGSMVNKIKSSGCDVVLSNVVGDSVIAF
YREYKNQGLTHDKLPICATVTSEIEIAAMGAEYAVGSFTSFPYFQAIDTDRNKAFIERYRKFVKDPKAVTHHALESSYFQ
VFLWKQAVEKAAEITPVAIREAVKGQEFDAPNGHVKIEAENLHTYLTPRIAQWLPDGQGKIIDAYKEPVMPLPYVAYGET
PTNLFCTGKGLDAAKLKT

Sequences:

>Translated_418_residues
MAVDRRKFLKSGALAGGALITSPWVFSTGAQAAGEIKVGVLFSLTGGLSIVEKSLHDATMMAISEINAAGGVKGMKISAI
AEDGASDPKTYNEKASKLVIQDKVPTVFGSYTSASRKAVLPVFEKRNNLYFYPTYYEGFECSKNVVYTGAVPNQQLSNFI
PWIIKTLGKKKFFIVGSNYIYPREMAKVSKILIEKNGGEWIADEYLELGHSEWGSMVNKIKSSGCDVVLSNVVGDSVIAF
YREYKNQGLTHDKLPICATVTSEIEIAAMGAEYAVGSFTSFPYFQAIDTDRNKAFIERYRKFVKDPKAVTHHALESSYFQ
VFLWKQAVEKAAEITPVAIREAVKGQEFDAPNGHVKIEAENLHTYLTPRIAQWLPDGQGKIIDAYKEPVMPLPYVAYGET
PTNLFCTGKGLDAAKLKT
>Mature_417_residues
AVDRRKFLKSGALAGGALITSPWVFSTGAQAAGEIKVGVLFSLTGGLSIVEKSLHDATMMAISEINAAGGVKGMKISAIA
EDGASDPKTYNEKASKLVIQDKVPTVFGSYTSASRKAVLPVFEKRNNLYFYPTYYEGFECSKNVVYTGAVPNQQLSNFIP
WIIKTLGKKKFFIVGSNYIYPREMAKVSKILIEKNGGEWIADEYLELGHSEWGSMVNKIKSSGCDVVLSNVVGDSVIAFY
REYKNQGLTHDKLPICATVTSEIEIAAMGAEYAVGSFTSFPYFQAIDTDRNKAFIERYRKFVKDPKAVTHHALESSYFQV
FLWKQAVEKAAEITPVAIREAVKGQEFDAPNGHVKIEAENLHTYLTPRIAQWLPDGQGKIIDAYKEPVMPLPYVAYGETP
TNLFCTGKGLDAAKLKT

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 46002; Mature: 45871

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVDRRKFLKSGALAGGALITSPWVFSTGAQAAGEIKVGVLFSLTGGLSIVEKSLHDATM
CCCCHHHHHHHCCCCCCEEEECCEEEECCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHH
MAISEINAAGGVKGMKISAIAEDGASDPKTYNEKASKLVIQDKVPTVFGSYTSASRKAVL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHEEEEECCCCHHHCCCCCCCCCEEC
PVFEKRNNLYFYPTYYEGFECSKNVVYTGAVPNQQLSNFIPWIIKTLGKKKFFIVGSNYI
HHHHCCCCEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCEE
YPREMAKVSKILIEKNGGEWIADEYLELGHSEWGSMVNKIKSSGCDVVLSNVVGDSVIAF
CHHHHHHHHHHHEECCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
YREYKNQGLTHDKLPICATVTSEIEIAAMGAEYAVGSFTSFPYFQAIDTDRNKAFIERYR
HHHHHHCCCCCCCCCEEEEECCCEEEEEECCCHHCCCCCCCCCEEEECCCCHHHHHHHHH
KFVKDPKAVTHHALESSYFQVFLWKQAVEKAAEITPVAIREAVKGQEFDAPNGHVKIEAE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEEEC
NLHTYLTPRIAQWLPDGQGKIIDAYKEPVMPLPYVAYGETPTNLFCTGKGLDAAKLKT
CCEEHHCHHHHHHCCCCCCCEEHHHHCCCCCCCEEEECCCCCCEEEECCCCCHHCCCC
>Mature Secondary Structure 
AVDRRKFLKSGALAGGALITSPWVFSTGAQAAGEIKVGVLFSLTGGLSIVEKSLHDATM
CCCHHHHHHHCCCCCCEEEECCEEEECCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHH
MAISEINAAGGVKGMKISAIAEDGASDPKTYNEKASKLVIQDKVPTVFGSYTSASRKAVL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHEEEEECCCCHHHCCCCCCCCCEEC
PVFEKRNNLYFYPTYYEGFECSKNVVYTGAVPNQQLSNFIPWIIKTLGKKKFFIVGSNYI
HHHHCCCCEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCEE
YPREMAKVSKILIEKNGGEWIADEYLELGHSEWGSMVNKIKSSGCDVVLSNVVGDSVIAF
CHHHHHHHHHHHEECCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
YREYKNQGLTHDKLPICATVTSEIEIAAMGAEYAVGSFTSFPYFQAIDTDRNKAFIERYR
HHHHHHCCCCCCCCCEEEEECCCEEEEEECCCHHCCCCCCCCCEEEECCCCHHHHHHHHH
KFVKDPKAVTHHALESSYFQVFLWKQAVEKAAEITPVAIREAVKGQEFDAPNGHVKIEAE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEEEC
NLHTYLTPRIAQWLPDGQGKIIDAYKEPVMPLPYVAYGETPTNLFCTGKGLDAAKLKT
CCEEHHCHHHHHHCCCCCCCEEHHHHCCCCCCCEEEECCCCCCEEEECCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]