Click here to switch to the map view.

The map label for this gene is braD [H]

Identifier: 146342706

GI number: 146342706

Start: 6091857

End: 6092750

Strand: Reverse

Name: braD [H]

Synonym: BRADO5873

Alternate gene names: 146342706

Gene position: 6092750-6091857 (Counterclockwise)

Preceding gene: 146342707

Following gene: 146342705

Centisome position: 81.71

GC content: 63.87

Gene sequence:

>894_bases
ATGCTCGAGGTTCTGTTTATCGGTCTCAGCCTGGGATCGATCCTGCTCCTGGTCGCGCTCGGTCTCGCCATCACCTATGG
CGCGATGGGCGTCATCAACATGGCGCATGGCGAGATGGTCATGGTCGGCGCCTACGTGACTGTGCTCTCCGGCCTGTGGC
TCGGCACCAACATCTTCGTCGCGATCCCGCTGGCCTTCATCGTCACCGCGCTGCTTGGGCTGCTGATCGAGCGCGTCGTG
GTGCGCAAGCTCTATGGCCGGCTGCTCGACACGCTGCTCGCGACCTGGGGCATCGCCATTCTGATTCAGCAGGCGGTGCG
GCTCGAATTCGGGCTGTCGTTCCTCGGCATTCACATCCAGGGCCTTGGGCCGGGTCTGCAGAACGTGAGTGTGCCCGACA
TCCTGCAGGGCACGTTCCGCTTTGCCGGCTCCGACATCAACCGTTACCGCGCCTTCATCATTCTCATCACTGCCATTCTC
GCCATTGCCACCTGGTTCGTGATCTACCGCACCGTGGCTGGCACGCAGGTCCGCGCCATCATCCGCAATCCGAAGATGGC
GGCGGCCTGCGGCATCGACGTCACCCGGGTCAACGCGCTGACGTTCGCATTCGGATCGGGGCTCGCCGGAGTGGCCGGCG
TGATGATGTCCGGCTTCAAGACGGTCTTCCCGGACATGGGCACGTCGATGGTGGTCGACGGTTTCCTCGTTGTCGTGATG
GGTGGCGTCGGCAGCCTGCTCGGCTCGGTCCTGTCGGCCGGCATTCTCGGTGAGATCAACGGCCTCGTTGCTGCGGCGAC
CAACGACATCCTCGCCCGCGCTGTCGTGTTCGGCGTCGTGATCGCCATCATCGTGCTCAAGCCGAAGGGCCTGTTTGCCC
TCAAGGGACGCTGA

Upstream 100 bases:

>100_bases
CGGCAAGGGGCTCGACGCGGCCAAGCTGAAAACCTGACGTCAAGGAAACCCTGTCCACCGCGCGCCTCCCTCGCGCGGTG
GATGGCGGGAGGCATCTGTC

Downstream 100 bases:

>100_bases
TCATGATGTCGAAGCGACGCATCGACTGGGCGATCTACGCGATCTTCATTGTCGCGATCATGCTCGTGCCGCTGATCATG
GACTCGTTCTGGCTCAACCG

Product: branched chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLEVLFIGLSLGSILLLVALGLAITYGAMGVINMAHGEMVMVGAYVTVLSGLWLGTNIFVAIPLAFIVTALLGLLIERVV
VRKLYGRLLDTLLATWGIAILIQQAVRLEFGLSFLGIHIQGLGPGLQNVSVPDILQGTFRFAGSDINRYRAFIILITAIL
AIATWFVIYRTVAGTQVRAIIRNPKMAAACGIDVTRVNALTFAFGSGLAGVAGVMMSGFKTVFPDMGTSMVVDGFLVVVM
GGVGSLLGSVLSAGILGEINGLVAAATNDILARAVVFGVVIAIIVLKPKGLFALKGR

Sequences:

>Translated_297_residues
MLEVLFIGLSLGSILLLVALGLAITYGAMGVINMAHGEMVMVGAYVTVLSGLWLGTNIFVAIPLAFIVTALLGLLIERVV
VRKLYGRLLDTLLATWGIAILIQQAVRLEFGLSFLGIHIQGLGPGLQNVSVPDILQGTFRFAGSDINRYRAFIILITAIL
AIATWFVIYRTVAGTQVRAIIRNPKMAAACGIDVTRVNALTFAFGSGLAGVAGVMMSGFKTVFPDMGTSMVVDGFLVVVM
GGVGSLLGSVLSAGILGEINGLVAAATNDILARAVVFGVVIAIIVLKPKGLFALKGR
>Mature_297_residues
MLEVLFIGLSLGSILLLVALGLAITYGAMGVINMAHGEMVMVGAYVTVLSGLWLGTNIFVAIPLAFIVTALLGLLIERVV
VRKLYGRLLDTLLATWGIAILIQQAVRLEFGLSFLGIHIQGLGPGLQNVSVPDILQGTFRFAGSDINRYRAFIILITAIL
AIATWFVIYRTVAGTQVRAIIRNPKMAAACGIDVTRVNALTFAFGSGLAGVAGVMMSGFKTVFPDMGTSMVVDGFLVVVM
GGVGSLLGSVLSAGILGEINGLVAAATNDILARAVVFGVVIAIIVLKPKGLFALKGR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=308, Percent_Identity=29.8701298701299, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31089; Mature: 31089

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEVLFIGLSLGSILLLVALGLAITYGAMGVINMAHGEMVMVGAYVTVLSGLWLGTNIFV
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEHHHHHHHHHHHHHHCCHHHH
AIPLAFIVTALLGLLIERVVVRKLYGRLLDTLLATWGIAILIQQAVRLEFGLSFLGIHIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
GLGPGLQNVSVPDILQGTFRFAGSDINRYRAFIILITAILAIATWFVIYRTVAGTQVRAI
ECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
IRNPKMAAACGIDVTRVNALTFAFGSGLAGVAGVMMSGFKTVFPDMGTSMVVDGFLVVVM
HCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GGVGSLLGSVLSAGILGEINGLVAAATNDILARAVVFGVVIAIIVLKPKGLFALKGR
HHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
>Mature Secondary Structure
MLEVLFIGLSLGSILLLVALGLAITYGAMGVINMAHGEMVMVGAYVTVLSGLWLGTNIFV
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEHHHHHHHHHHHHHHCCHHHH
AIPLAFIVTALLGLLIERVVVRKLYGRLLDTLLATWGIAILIQQAVRLEFGLSFLGIHIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
GLGPGLQNVSVPDILQGTFRFAGSDINRYRAFIILITAILAIATWFVIYRTVAGTQVRAI
ECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
IRNPKMAAACGIDVTRVNALTFAFGSGLAGVAGVMMSGFKTVFPDMGTSMVVDGFLVVVM
HCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GGVGSLLGSVLSAGILGEINGLVAAATNDILARAVVFGVVIAIIVLKPKGLFALKGR
HHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]