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The map label for this gene is glgX [H]

Identifier: 146342651

GI number: 146342651

Start: 6030546

End: 6032621

Strand: Direct

Name: glgX [H]

Synonym: BRADO5818

Alternate gene names: 146342651

Gene position: 6030546-6032621 (Clockwise)

Preceding gene: 146342650

Following gene: 146342652

Centisome position: 80.88

GC content: 65.22

Gene sequence:

>2076_bases
ATGCGCCTGGCCGCAGGAAGTCACGCCCGCCTCGGTGCCACCTGGGACGGGCGAGGGACCAACTTTGCCCTGTTCTCCGC
CAATGCCGAGAAGGTCGAGCTCTGCCTGTTCGACAGCCAGGGCCGCCGCGAGATCGAGCGCATCGAGCTGCCGGAGCGGA
CGGAGGACGTCTGGCACGGCTATCTCAACGACGTCTCGCCCGGGCAGCTCTACGGCTACCGCGTCTATGGCCCCTATGAG
CCGGAGCGCGGCCACCGCTTCAATGCCAACAAGCTGCTGCTCGACCCCTATGCCAAGCGGCTCGCCGGCCGCCTTGTCTG
GAGCGACGCGCATTTCGCCTACCGTGCCGGCTCGCCGCGCGAGGACCTCTCCTTCGACCGCCGCGACAATGCGCGCGGCA
TGCCGAAGGCCGTCGTCATCGACGAGACCTTCAACTGGGGCCGGCGCGAGATGCGCCCGCAGATCCCGTGGGAGGACACG
ATCATCTACGAGGCCCACGTCAAGGGCCTGACCAACAGACGCGACGACGTGCCGCCGAACCTGCGCGGCACCTATGGCGG
GCTGTCGTCGCCGGCGATGATCAAGCATCTGAAGCGCATGGGCGTCACCACGATCGAGCTGCTGCCGATCCACGCCTTCA
TCGACGACCGCATGCTGGTCGAGAAGAAGCTGGTCAACTATTGGGGCTACAACACCATCTCGTTCTTCGCGCCGGAGCAG
CGCTACGCGCAGGACAACCCGCTCGACGCTTTCAGGACCACGGTGGCGCGGCTGCACGATGCCGGCATCGAGGTGATGCT
CGACGTCGTCTACAACCACACTGCCGAGGGCAATCATCTCGGCCCGACGCTGTGCTACCGCGGCATCGACAACGCCTCCT
ACTACTGGCTGAAGCCGGACAATCCGCGCTTCTATGACGACTTCACCGGCTGCGGCTCGTCGGTCAACCTGACGCATCCA
CGCGTGCTGCAGATGGTGATGGACTCTCTTCGCTACTGGGTCGAGGTCTGCCATGTCGACGGCTTCCGCTTCGACCTCGC
CACCACGCTCGCCCGCGAGAAGCACGGCTTCGACCGGCGCAGCGGCTTCCTCACCGCGATCCGCCAGGACCCGGTGCTCG
CCGGCGTCAAGCTGGTCGCCGAGCCCTGGGACGTCGGCCTTGGCGGCTATCAGGTCGGCGCGTTCCCGTCGCAATGGTCG
GAATGGAACGACCGCTATCGCAGCGCCATGCGCCGCTACTGGAGCGGCGAAGGCAGCCTGATCGGCGAGGTGTCGAGCCG
CATGACCGGCTCCTCTGACATCTTCAATCACGACGGCCGCACCCAGCGCGCCAGCGTCAACCACGTCACCGTCCACGATG
GCTTCACCCTGGCCGATCTCTTCGCCTACAACAGCAAGCATAACGAGGCGAACGGCGAGGACAATCGCGACGGCTCCAAC
GACAACCACAGCAACAATTTCGGTCACGAGGGTCCGACCGACGACGTCGTGATCAACGCGCTGCGCCGGCAGTCGCGCAA
GAACCAGCTCGCCTGTCTGTTCCTCGCACAAGGCTTGCCGCTTTTGCTCGCGGGCGACGAGGTCGGCAACTCGCAAGCCG
GCAACAACAACGCCTATTGCCAGGACAACGAGGTCGGCTGGATCGACTGGAGCGGCATGGGCCGCGAGGGCGACGACCTC
ATCGATTTCATCGCGCATATGACCGAGTTGCGCCGCCGCTTCGGCCAGATCCGTGCGCGGCGCTGGCTGGACGGCCGCCG
CTCGGACGGCAGCTTCGGCGTGTTGTGGCTGACGCCTTCGGCGGAGGAGATGACGCAAACCGACTGGACCTTTCCCGATG
GACGATTTCTCGCCTATGTGCTCGCCCCGGTGGAACAGGAGCAGGCTCCGATCTTTATCGTTCTGAACGCGGCCCCCGAA
GAGATCGGATTCAAATTGCCCAAGCTCGCCGAAACCAAGATTTGGCAACAGGTTCTGAACACCACCGAAGCCCAGCAGAA
GCCGATCGACTTCGCGTCCGGCACGGACCTCAAGGCCCCGCCCCGCTCCGTCCTGGCGTTTGCGGGCCTGTCATGA

Upstream 100 bases:

>100_bases
GTCGGCAATGTCGGCCAGGTCCATGCCTCTGAAGATCAGCACCTCAACCTCATCCTCCCGCCCATGGCAGCGGTATTTCT
CGTCCCGGAAGCCTGACGCG

Downstream 100 bases:

>100_bases
CCGCGCGGCAGTTCGGACCCGCGATCACGGCAAACGGCACGACCTTCCGGCTGTGGGCGCCCGCGGCGAAGCGGGTTGAT
CTGGTGCTCGACGGTCAACA

Product: glycosyl hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MRLAAGSHARLGATWDGRGTNFALFSANAEKVELCLFDSQGRREIERIELPERTEDVWHGYLNDVSPGQLYGYRVYGPYE
PERGHRFNANKLLLDPYAKRLAGRLVWSDAHFAYRAGSPREDLSFDRRDNARGMPKAVVIDETFNWGRREMRPQIPWEDT
IIYEAHVKGLTNRRDDVPPNLRGTYGGLSSPAMIKHLKRMGVTTIELLPIHAFIDDRMLVEKKLVNYWGYNTISFFAPEQ
RYAQDNPLDAFRTTVARLHDAGIEVMLDVVYNHTAEGNHLGPTLCYRGIDNASYYWLKPDNPRFYDDFTGCGSSVNLTHP
RVLQMVMDSLRYWVEVCHVDGFRFDLATTLAREKHGFDRRSGFLTAIRQDPVLAGVKLVAEPWDVGLGGYQVGAFPSQWS
EWNDRYRSAMRRYWSGEGSLIGEVSSRMTGSSDIFNHDGRTQRASVNHVTVHDGFTLADLFAYNSKHNEANGEDNRDGSN
DNHSNNFGHEGPTDDVVINALRRQSRKNQLACLFLAQGLPLLLAGDEVGNSQAGNNNAYCQDNEVGWIDWSGMGREGDDL
IDFIAHMTELRRRFGQIRARRWLDGRRSDGSFGVLWLTPSAEEMTQTDWTFPDGRFLAYVLAPVEQEQAPIFIVLNAAPE
EIGFKLPKLAETKIWQQVLNTTEAQQKPIDFASGTDLKAPPRSVLAFAGLS

Sequences:

>Translated_691_residues
MRLAAGSHARLGATWDGRGTNFALFSANAEKVELCLFDSQGRREIERIELPERTEDVWHGYLNDVSPGQLYGYRVYGPYE
PERGHRFNANKLLLDPYAKRLAGRLVWSDAHFAYRAGSPREDLSFDRRDNARGMPKAVVIDETFNWGRREMRPQIPWEDT
IIYEAHVKGLTNRRDDVPPNLRGTYGGLSSPAMIKHLKRMGVTTIELLPIHAFIDDRMLVEKKLVNYWGYNTISFFAPEQ
RYAQDNPLDAFRTTVARLHDAGIEVMLDVVYNHTAEGNHLGPTLCYRGIDNASYYWLKPDNPRFYDDFTGCGSSVNLTHP
RVLQMVMDSLRYWVEVCHVDGFRFDLATTLAREKHGFDRRSGFLTAIRQDPVLAGVKLVAEPWDVGLGGYQVGAFPSQWS
EWNDRYRSAMRRYWSGEGSLIGEVSSRMTGSSDIFNHDGRTQRASVNHVTVHDGFTLADLFAYNSKHNEANGEDNRDGSN
DNHSNNFGHEGPTDDVVINALRRQSRKNQLACLFLAQGLPLLLAGDEVGNSQAGNNNAYCQDNEVGWIDWSGMGREGDDL
IDFIAHMTELRRRFGQIRARRWLDGRRSDGSFGVLWLTPSAEEMTQTDWTFPDGRFLAYVLAPVEQEQAPIFIVLNAAPE
EIGFKLPKLAETKIWQQVLNTTEAQQKPIDFASGTDLKAPPRSVLAFAGLS
>Mature_691_residues
MRLAAGSHARLGATWDGRGTNFALFSANAEKVELCLFDSQGRREIERIELPERTEDVWHGYLNDVSPGQLYGYRVYGPYE
PERGHRFNANKLLLDPYAKRLAGRLVWSDAHFAYRAGSPREDLSFDRRDNARGMPKAVVIDETFNWGRREMRPQIPWEDT
IIYEAHVKGLTNRRDDVPPNLRGTYGGLSSPAMIKHLKRMGVTTIELLPIHAFIDDRMLVEKKLVNYWGYNTISFFAPEQ
RYAQDNPLDAFRTTVARLHDAGIEVMLDVVYNHTAEGNHLGPTLCYRGIDNASYYWLKPDNPRFYDDFTGCGSSVNLTHP
RVLQMVMDSLRYWVEVCHVDGFRFDLATTLAREKHGFDRRSGFLTAIRQDPVLAGVKLVAEPWDVGLGGYQVGAFPSQWS
EWNDRYRSAMRRYWSGEGSLIGEVSSRMTGSSDIFNHDGRTQRASVNHVTVHDGFTLADLFAYNSKHNEANGEDNRDGSN
DNHSNNFGHEGPTDDVVINALRRQSRKNQLACLFLAQGLPLLLAGDEVGNSQAGNNNAYCQDNEVGWIDWSGMGREGDDL
IDFIAHMTELRRRFGQIRARRWLDGRRSDGSFGVLWLTPSAEEMTQTDWTFPDGRFLAYVLAPVEQEQAPIFIVLNAAPE
EIGFKLPKLAETKIWQQVLNTTEAQQKPIDFASGTDLKAPPRSVLAFAGLS

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=670, Percent_Identity=45.5223880597015, Blast_Score=558, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78237; Mature: 78237

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAAGSHARLGATWDGRGTNFALFSANAEKVELCLFDSQGRREIERIELPERTEDVWHG
CCCCCCCCCEECCEECCCCCEEEEEECCCCEEEEEEECCCCCHHHHHCCCCCHHHHHHHH
YLNDVSPGQLYGYRVYGPYEPERGHRFNANKLLLDPYAKRLAGRLVWSDAHFAYRAGSPR
HHCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHEEECCCCEEEECCCCH
EDLSFDRRDNARGMPKAVVIDETFNWGRREMRPQIPWEDTIIYEAHVKGLTNRRDDVPPN
HHCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCEEEEEEHHCCCCCCCCCCCCC
LRGTYGGLSSPAMIKHLKRMGVTTIELLPIHAFIDDRMLVEKKLVNYWGYNTISFFAPEQ
CCCCCCCCCCCHHHHHHHHCCCEEEEEEEEEHHHCCHHHHHHHHHHHCCCCEEEEECCHH
RYAQDNPLDAFRTTVARLHDAGIEVMLDVVYNHTAEGNHLGPTLCYRGIDNASYYWLKPD
HCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEECCC
NPRFYDDFTGCGSSVNLTHPRVLQMVMDSLRYWVEVCHVDGFRFDLATTLAREKHGFDRR
CCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHH
SGFLTAIRQDPVLAGVKLVAEPWDVGLGGYQVGAFPSQWSEWNDRYRSAMRRYWSGEGSL
CCCEEEHHCCCHHCCCEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCE
IGEVSSRMTGSSDIFNHDGRTQRASVNHVTVHDGFTLADLFAYNSKHNEANGEDNRDGSN
EHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCC
DNHSNNFGHEGPTDDVVINALRRQSRKNQLACLFLAQGLPLLLAGDEVGNSQAGNNNAYC
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCC
QDNEVGWIDWSGMGREGDDLIDFIAHMTELRRRFGQIRARRWLDGRRSDGSFGVLWLTPS
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
AEEMTQTDWTFPDGRFLAYVLAPVEQEQAPIFIVLNAAPEEIGFKLPKLAETKIWQQVLN
HHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCHHHCCCCCCHHHHHHHHHHHH
TTEAQQKPIDFASGTDLKAPPRSVLAFAGLS
HHHHHHCCCCCCCCCCCCCCHHHHHEECCCC
>Mature Secondary Structure
MRLAAGSHARLGATWDGRGTNFALFSANAEKVELCLFDSQGRREIERIELPERTEDVWHG
CCCCCCCCCEECCEECCCCCEEEEEECCCCEEEEEEECCCCCHHHHHCCCCCHHHHHHHH
YLNDVSPGQLYGYRVYGPYEPERGHRFNANKLLLDPYAKRLAGRLVWSDAHFAYRAGSPR
HHCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHEEECCCCEEEECCCCH
EDLSFDRRDNARGMPKAVVIDETFNWGRREMRPQIPWEDTIIYEAHVKGLTNRRDDVPPN
HHCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCEEEEEEHHCCCCCCCCCCCCC
LRGTYGGLSSPAMIKHLKRMGVTTIELLPIHAFIDDRMLVEKKLVNYWGYNTISFFAPEQ
CCCCCCCCCCCHHHHHHHHCCCEEEEEEEEEHHHCCHHHHHHHHHHHCCCCEEEEECCHH
RYAQDNPLDAFRTTVARLHDAGIEVMLDVVYNHTAEGNHLGPTLCYRGIDNASYYWLKPD
HCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEECCC
NPRFYDDFTGCGSSVNLTHPRVLQMVMDSLRYWVEVCHVDGFRFDLATTLAREKHGFDRR
CCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHH
SGFLTAIRQDPVLAGVKLVAEPWDVGLGGYQVGAFPSQWSEWNDRYRSAMRRYWSGEGSL
CCCEEEHHCCCHHCCCEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCE
IGEVSSRMTGSSDIFNHDGRTQRASVNHVTVHDGFTLADLFAYNSKHNEANGEDNRDGSN
EHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCC
DNHSNNFGHEGPTDDVVINALRRQSRKNQLACLFLAQGLPLLLAGDEVGNSQAGNNNAYC
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCC
QDNEVGWIDWSGMGREGDDLIDFIAHMTELRRRFGQIRARRWLDGRRSDGSFGVLWLTPS
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
AEEMTQTDWTFPDGRFLAYVLAPVEQEQAPIFIVLNAAPEEIGFKLPKLAETKIWQQVLN
HHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCHHHCCCCCCHHHHHHHHHHHH
TTEAQQKPIDFASGTDLKAPPRSVLAFAGLS
HHHHHHCCCCCCCCCCCCCCHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]