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The map label for this gene is phnJ [H]

Identifier: 146342201

GI number: 146342201

Start: 5561249

End: 5562133

Strand: Reverse

Name: phnJ [H]

Synonym: BRADO5353

Alternate gene names: 146342201

Gene position: 5562133-5561249 (Counterclockwise)

Preceding gene: 146342202

Following gene: 146342200

Centisome position: 74.59

GC content: 63.16

Gene sequence:

>885_bases
ATGAACGCGCCGACCTACAACTTCGCCTATCTCGACGAGCAGACCAAGCGGATGATCCGCCGCGCGATCCTGAAGGCGAT
CGCGATCCCGGGCTACCAGGTGCCGTTCGCGAGCCGCGAGATGCCGATGCCCTATGGCTGGGGCACCGGCGGCGTCCAGG
TCACCGCGGCCATCCTCGGTCCCGACGACGTGCTCAAGGTGATCGATCAGGGCTCGGACGACACCACCAATGCGATCTCG
ATCCGTAAGTTCTTCGCCAAGACCGCGGGCGTCGCGACCACGACGTCGACGGATGCTGCGACCGTGATCCAGACCCGCCA
CCGCATTCCCGAGGCGCCGCTGCGCGCGGGACAGGTGCTGGTGTATCAGGTGCCGATCCCGGAGCCGCTGCGGTTCCTCG
AACCGCGCGAGACCGAGACGCGGCGCATGCATGCGTTGGCCGAATATGGTCTGATGCACGTCAAGCTGTATGAGGACATT
GCACGCTTCGGCCACATCGCGACCTCCTATGCCTATCCGGTCAAGGTCAATGCGCGCTATGTAATGGACCCGTCGCCGAC
GCCGAAGTTCGACAATCCCAAGATGGACAATTGCCCGGCCTTGCAATTGTTCGGTGCCGGCCGCGAAAAGCGCATCTACG
CTATTCCGCCTTATACGTCGGTGGTGTCGCTCGATTTCGAGGACCATCCGTTCACGCGCTACAAGTTCGATGCGCCCTGT
GCGCTGTGTGGAGCGGAAGATTCTTATCTCGACGAGATCGTGACCAACAACAAGGGCAGCCGGATGTACGTCTGCTCCGA
TACCGACTATTGCGAGGGCCGCCAGGCGGCCGGACATTGCGGCACCGAGAGCGCCGCTCCGCACAAGGAGGCGGCCCATG
GCTGA

Upstream 100 bases:

>100_bases
CGCACTACGTCGACTTCCAGTCCGAACTCGGCCTGCTGCGAAAACTGCGTCAGGAATTTGCCGACGCCAATGCCGCTCCC
GCGATGAAGGAGGCTGCGGA

Downstream 100 bases:

>100_bases
GGTGACCGGCGCATCGGATCAGCCGCTGCTCGCCGCCGAGGGGCTGACCAAGAGCTATGGCCGTCTCGCGGCCTGCCGCG
ACGTATCCTTCGTGCTCTAT

Product: putative PhnJ protein, phosphonate metabolism

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MNAPTYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILGPDDVLKVIDQGSDDTTNAIS
IRKFFAKTAGVATTTSTDAATVIQTRHRIPEAPLRAGQVLVYQVPIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDI
ARFGHIATSYAYPVKVNARYVMDPSPTPKFDNPKMDNCPALQLFGAGREKRIYAIPPYTSVVSLDFEDHPFTRYKFDAPC
ALCGAEDSYLDEIVTNNKGSRMYVCSDTDYCEGRQAAGHCGTESAAPHKEAAHG

Sequences:

>Translated_294_residues
MNAPTYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILGPDDVLKVIDQGSDDTTNAIS
IRKFFAKTAGVATTTSTDAATVIQTRHRIPEAPLRAGQVLVYQVPIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDI
ARFGHIATSYAYPVKVNARYVMDPSPTPKFDNPKMDNCPALQLFGAGREKRIYAIPPYTSVVSLDFEDHPFTRYKFDAPC
ALCGAEDSYLDEIVTNNKGSRMYVCSDTDYCEGRQAAGHCGTESAAPHKEAAHG
>Mature_294_residues
MNAPTYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILGPDDVLKVIDQGSDDTTNAIS
IRKFFAKTAGVATTTSTDAATVIQTRHRIPEAPLRAGQVLVYQVPIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDI
ARFGHIATSYAYPVKVNARYVMDPSPTPKFDNPKMDNCPALQLFGAGREKRIYAIPPYTSVVSLDFEDHPFTRYKFDAPC
ALCGAEDSYLDEIVTNNKGSRMYVCSDTDYCEGRQAAGHCGTESAAPHKEAAHG

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=278, Percent_Identity=74.8201438848921, Blast_Score=456, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 32577; Mature: 32577

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPTYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILG
CCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEC
PDDVLKVIDQGSDDTTNAISIRKFFAKTAGVATTTSTDAATVIQTRHRIPEAPLRAGQVL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCCCCCCEE
VYQVPIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDIARFGHIATSYAYPVKVNARY
EEECCCCCHHHHCCCCCHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHCEEEEEEECEEE
VMDPSPTPKFDNPKMDNCPALQLFGAGREKRIYAIPPYTSVVSLDFEDHPFTRYKFDAPC
EECCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEEEEECCCCCCCEEEEECCCE
ALCGAEDSYLDEIVTNNKGSRMYVCSDTDYCEGRQAAGHCGTESAAPHKEAAHG
EEECCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MNAPTYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILG
CCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEC
PDDVLKVIDQGSDDTTNAISIRKFFAKTAGVATTTSTDAATVIQTRHRIPEAPLRAGQVL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCCCCCCEE
VYQVPIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDIARFGHIATSYAYPVKVNARY
EEECCCCCHHHHCCCCCHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHCEEEEEEECEEE
VMDPSPTPKFDNPKMDNCPALQLFGAGREKRIYAIPPYTSVVSLDFEDHPFTRYKFDAPC
EECCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEEEEECCCCCCCEEEEECCCE
ALCGAEDSYLDEIVTNNKGSRMYVCSDTDYCEGRQAAGHCGTESAAPHKEAAHG
EEECCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]