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The map label for this gene is phnI [H]

Identifier: 146342202

GI number: 146342202

Start: 5562130

End: 5563233

Strand: Reverse

Name: phnI [H]

Synonym: BRADO5354

Alternate gene names: 146342202

Gene position: 5563233-5562130 (Counterclockwise)

Preceding gene: 146342203

Following gene: 146342201

Centisome position: 74.61

GC content: 66.39

Gene sequence:

>1104_bases
ATGTATGTAGCGGTGAAGGGTGGCGAGCGCGCCATCGAGAATGCGCACCGGCTGCTGGCGCATGAGCGTCGCGGCGACCG
CAGCGTGCCGGAGCTGTCGCTGTCGCAGATCTCCGAGCAGCTCGGGCTCGCGGTCGATCGCGTCATGAGCGAGGGCTCGC
TGTATGACCGCGAGCTGGCGGCACTCGCGATCAAACAGGCGCGCGGCGATCTGATCGAGGCGATCTTTCTGGTGCGTGCC
TTCCGCGCGACCTTGCCGCGCTTCGGTGCGACCGAGCCGGTCAATACCGGCGGGATGCGCGTGCAGCGGCGGGTCTCGTC
CACCTTCAAGGACATTCCCGGCGGGCAGGTGCTGGGACCGACTTTCGACTACACGCATCGGCTGCTCGACCCGGGCCTGG
CACAAGGCGGCGATCCGGAAAATCCGGCCCTCGCTGCAACGTCCGATGTCCCGATGCCGCGCGTGACCGATATCCTCGGT
CAGGACGGACTGATCGAGCCGTCTCCCGCAGCGGAGGCCGATGCGCCGGTCGGCGATCTCACGCGCGATCCCCTGAGCTT
TCCGGCCGGTCGCGATCTGCGGCTGCAGAATCTGGCGCGCGGCGACGAGGGCTTCCTGCTCGCGCTGGGCTACTCGACCC
AGCGCGGCTACGGCCGCAATCATCCCTTCGCAGGCGAGATCCGCTTCGGCGAGGTCGAGGTAGAGTTCTTTGCTGAGGAG
ATCGGCTTCGCAGTGCCGCTCGGATCGATCGAGTTGACCGAATGCCAGATGGTCAATCAGTTCAAGGGCTCGGCCACCGA
GCCGCCGTGTTTCACGCGCGGTTACGGGCTGGCGTTCGGCCAGAGCGAGCGCAAGACGATGTCGATGGCGCTGGTCGATC
GCGCGCTGCGCGCGCGCGAACTCGGTGAGGACGTCATCGCGCCGGCGCAGGACGAGGAGTTCGTGATGTCGCACTCCGAC
AACGTCCAAGCGACCGGCTTCGTCGAGCATCTCAAGCTGCCGCACTACGTCGACTTCCAGTCCGAACTCGGCCTGCTGCG
AAAACTGCGTCAGGAATTTGCCGACGCCAATGCCGCTCCCGCGATGAAGGAGGCTGCGGAATGA

Upstream 100 bases:

>100_bases
AGGCGCTGTTTCCGCGCGGCATCGACGTGATCCTGGTCGATGATCGCTCGCTGGTCGCAATCCCGCGCACGACGCGCCTC
GCAGCCTCGGGAGTCTGACC

Downstream 100 bases:

>100_bases
ACGCGCCGACCTACAACTTCGCCTATCTCGACGAGCAGACCAAGCGGATGATCCGCCGCGCGATCCTGAAGGCGATCGCG
ATCCCGGGCTACCAGGTGCC

Product: putative PhnI protein, phosphonate metabolism

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MYVAVKGGERAIENAHRLLAHERRGDRSVPELSLSQISEQLGLAVDRVMSEGSLYDRELAALAIKQARGDLIEAIFLVRA
FRATLPRFGATEPVNTGGMRVQRRVSSTFKDIPGGQVLGPTFDYTHRLLDPGLAQGGDPENPALAATSDVPMPRVTDILG
QDGLIEPSPAAEADAPVGDLTRDPLSFPAGRDLRLQNLARGDEGFLLALGYSTQRGYGRNHPFAGEIRFGEVEVEFFAEE
IGFAVPLGSIELTECQMVNQFKGSATEPPCFTRGYGLAFGQSERKTMSMALVDRALRARELGEDVIAPAQDEEFVMSHSD
NVQATGFVEHLKLPHYVDFQSELGLLRKLRQEFADANAAPAMKEAAE

Sequences:

>Translated_367_residues
MYVAVKGGERAIENAHRLLAHERRGDRSVPELSLSQISEQLGLAVDRVMSEGSLYDRELAALAIKQARGDLIEAIFLVRA
FRATLPRFGATEPVNTGGMRVQRRVSSTFKDIPGGQVLGPTFDYTHRLLDPGLAQGGDPENPALAATSDVPMPRVTDILG
QDGLIEPSPAAEADAPVGDLTRDPLSFPAGRDLRLQNLARGDEGFLLALGYSTQRGYGRNHPFAGEIRFGEVEVEFFAEE
IGFAVPLGSIELTECQMVNQFKGSATEPPCFTRGYGLAFGQSERKTMSMALVDRALRARELGEDVIAPAQDEEFVMSHSD
NVQATGFVEHLKLPHYVDFQSELGLLRKLRQEFADANAAPAMKEAAE
>Mature_367_residues
MYVAVKGGERAIENAHRLLAHERRGDRSVPELSLSQISEQLGLAVDRVMSEGSLYDRELAALAIKQARGDLIEAIFLVRA
FRATLPRFGATEPVNTGGMRVQRRVSSTFKDIPGGQVLGPTFDYTHRLLDPGLAQGGDPENPALAATSDVPMPRVTDILG
QDGLIEPSPAAEADAPVGDLTRDPLSFPAGRDLRLQNLARGDEGFLLALGYSTQRGYGRNHPFAGEIRFGEVEVEFFAEE
IGFAVPLGSIELTECQMVNQFKGSATEPPCFTRGYGLAFGQSERKTMSMALVDRALRARELGEDVIAPAQDEEFVMSHSD
NVQATGFVEHLKLPHYVDFQSELGLLRKLRQEFADANAAPAMKEAAE

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3626

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnI family [H]

Homologues:

Organism=Escherichia coli, GI1790537, Length=354, Percent_Identity=60.4519774011299, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008773 [H]

Pfam domain/function: PF05861 PhnI [H]

EC number: NA

Molecular weight: Translated: 39983; Mature: 39983

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVAVKGGERAIENAHRLLAHERRGDRSVPELSLSQISEQLGLAVDRVMSEGSLYDRELA
CEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ALAIKQARGDLIEAIFLVRAFRATLPRFGATEPVNTGGMRVQRRVSSTFKDIPGGQVLGP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECC
TFDYTHRLLDPGLAQGGDPENPALAATSDVPMPRVTDILGQDGLIEPSPAAEADAPVGDL
CHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCC
TRDPLSFPAGRDLRLQNLARGDEGFLLALGYSTQRGYGRNHPFAGEIRFGEVEVEFFAEE
CCCCCCCCCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHH
IGFAVPLGSIELTECQMVNQFKGSATEPPCFTRGYGLAFGQSERKTMSMALVDRALRARE
HCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHH
LGEDVIAPAQDEEFVMSHSDNVQATGFVEHLKLPHYVDFQSELGLLRKLRQEFADANAAP
HCHHHCCCCCCCHHHHCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
AMKEAAE
CHHHHCC
>Mature Secondary Structure
MYVAVKGGERAIENAHRLLAHERRGDRSVPELSLSQISEQLGLAVDRVMSEGSLYDRELA
CEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ALAIKQARGDLIEAIFLVRAFRATLPRFGATEPVNTGGMRVQRRVSSTFKDIPGGQVLGP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECC
TFDYTHRLLDPGLAQGGDPENPALAATSDVPMPRVTDILGQDGLIEPSPAAEADAPVGDL
CHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCC
TRDPLSFPAGRDLRLQNLARGDEGFLLALGYSTQRGYGRNHPFAGEIRFGEVEVEFFAEE
CCCCCCCCCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHH
IGFAVPLGSIELTECQMVNQFKGSATEPPCFTRGYGLAFGQSERKTMSMALVDRALRARE
HCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHH
LGEDVIAPAQDEEFVMSHSDNVQATGFVEHLKLPHYVDFQSELGLLRKLRQEFADANAAP
HCHHHCCCCCCCHHHHCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
AMKEAAE
CHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]