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The map label for this gene is phnM [H]

Identifier: 146342198

GI number: 146342198

Start: 5558578

End: 5559735

Strand: Reverse

Name: phnM [H]

Synonym: BRADO5350

Alternate gene names: 146342198

Gene position: 5559735-5558578 (Counterclockwise)

Preceding gene: 146342199

Following gene: 146342197

Centisome position: 74.56

GC content: 67.79

Gene sequence:

>1158_bases
ATGAGCGCGAACATGTCCGACACCGTGATAGGCAACGCCCGGCTGGTGCTCGCCGACCGTGTCATCGAGCAGGGGTGGGT
GGCGTTGGCCGGAGGCCGGATCGCCGAGATCGGCGAAGGCGCCGCGCCCGCTGGCAGTCATGACGCAGGCGGCGATCTGC
TGATGCCCGGGCTTGTCGAGTTGCACACCGATCACCTCGAAGCGCATTTCGTGCCGCGCCCGAAAGTGTATTGGGATCCG
GTCGCCGCGGTGATTTCCTATGACGGACAGCTCGCGACGGCCGGCATCACCACGGTGCTGGATTCGCTGCGCGTCTGGAG
CGAGGAGGGCGCCGAAGGCGTCGATGGCCAGGCCAATGTGCTGGCGGATGCGATCGCGCGCGCGCGCGACGCACGCCTGC
TGCGCGCCGATCATTTCCTGCATCTGCGCTGCGAAGTGCCGATGCCGACCGTGGTCGAGGAGGCAAAGCAGTTGGCGGGC
CGCTCCGATGTCCGGCTGATGTCGCTGATGGACCACACGCCGGGCCAGCGCCAGTTCCGCGACGAGGGCAAGCTGCGGGA
CTACTACCGCGGCAAGAGCGGCGGCAAGACCGACGCGGAGCTCGACGTGATGTTCGCGCGTCGCTTCGCCTATCAGCAGG
CCTATGCGGCAGCGAACATGCGCGACATCGTCGCGCTCGCCAAGACCCATGACGTGCCGCTCGCCAGCCATGACGACACC
ACCGACGAGAACGTCGCCGACGCGATCAAGGACGGCATCGCCGTGGCGGAATTTCCGACCACGATGGAAGCGGCACGCGG
ACTTCACGAGGCTGGCATCAGCATCCTGATGGGTGCGCCCAACGTGGTCCGCGGCGGCTCGCATTCCGGTAACATTGCAG
CTGTCGATCTCGCGCGCGAGGGTCTGCTCGACATTCTGTCGTCGGACTATGTGCCGTCGAGCCTGCTGATGGGCGCGCTG
CAACTGCCACGGCGCGTCCCGGCGATCGACCTCGCTGCCGCGGTTCGCACCGTCACCAAGGCCCCGGCCGACGCGGTCGG
CCTGCGTGACCGCGGCGAGATCGCTCCTGGCAAGCGCGCGGATCTGATCCGCGTTCACGTCGCCGGCGACCATGCCGTCG
TACGCAGCGTCTGGCGGGAAGGGCTGCGTGTGGCATGA

Upstream 100 bases:

>100_bases
ATTGCGATCGTGCACGACGATGATATTCGTGCTGCCATCGCGGACCGCACGGTGGATGTGACGAATTTCGCAGCGGCGGC
GTAGCAGGCAGAGGGAGCAG

Downstream 100 bases:

>100_bases
GCGCGGCGTCGACTGGCTCGGATCACAACGCCCGCCTTGGTCCGGGCCGGCTGGTGCTCGTCGTCGGACCGAGCGGCGCG
GGCAAGGACACGTTGATCAA

Product: putative metal-dependent hydrolase involved in phosphonate metabolism/PhnM protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSANMSDTVIGNARLVLADRVIEQGWVALAGGRIAEIGEGAAPAGSHDAGGDLLMPGLVELHTDHLEAHFVPRPKVYWDP
VAAVISYDGQLATAGITTVLDSLRVWSEEGAEGVDGQANVLADAIARARDARLLRADHFLHLRCEVPMPTVVEEAKQLAG
RSDVRLMSLMDHTPGQRQFRDEGKLRDYYRGKSGGKTDAELDVMFARRFAYQQAYAAANMRDIVALAKTHDVPLASHDDT
TDENVADAIKDGIAVAEFPTTMEAARGLHEAGISILMGAPNVVRGGSHSGNIAAVDLAREGLLDILSSDYVPSSLLMGAL
QLPRRVPAIDLAAAVRTVTKAPADAVGLRDRGEIAPGKRADLIRVHVAGDHAVVRSVWREGLRVA

Sequences:

>Translated_385_residues
MSANMSDTVIGNARLVLADRVIEQGWVALAGGRIAEIGEGAAPAGSHDAGGDLLMPGLVELHTDHLEAHFVPRPKVYWDP
VAAVISYDGQLATAGITTVLDSLRVWSEEGAEGVDGQANVLADAIARARDARLLRADHFLHLRCEVPMPTVVEEAKQLAG
RSDVRLMSLMDHTPGQRQFRDEGKLRDYYRGKSGGKTDAELDVMFARRFAYQQAYAAANMRDIVALAKTHDVPLASHDDT
TDENVADAIKDGIAVAEFPTTMEAARGLHEAGISILMGAPNVVRGGSHSGNIAAVDLAREGLLDILSSDYVPSSLLMGAL
QLPRRVPAIDLAAAVRTVTKAPADAVGLRDRGEIAPGKRADLIRVHVAGDHAVVRSVWREGLRVA
>Mature_384_residues
SANMSDTVIGNARLVLADRVIEQGWVALAGGRIAEIGEGAAPAGSHDAGGDLLMPGLVELHTDHLEAHFVPRPKVYWDPV
AAVISYDGQLATAGITTVLDSLRVWSEEGAEGVDGQANVLADAIARARDARLLRADHFLHLRCEVPMPTVVEEAKQLAGR
SDVRLMSLMDHTPGQRQFRDEGKLRDYYRGKSGGKTDAELDVMFARRFAYQQAYAAANMRDIVALAKTHDVPLASHDDTT
DENVADAIKDGIAVAEFPTTMEAARGLHEAGISILMGAPNVVRGGSHSGNIAAVDLAREGLLDILSSDYVPSSLLMGALQ
LPRRVPAIDLAAAVRTVTKAPADAVGLRDRGEIAPGKRADLIRVHVAGDHAVVRSVWREGLRVA

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3454

COG function: function code P; Metal-dependent hydrolase involved in phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790533, Length=379, Percent_Identity=43.2717678100264, Blast_Score=291, Evalue=5e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059
- InterPro:   IPR012696 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: NA

Molecular weight: Translated: 41109; Mature: 40978

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANMSDTVIGNARLVLADRVIEQGWVALAGGRIAEIGEGAAPAGSHDAGGDLLMPGLVE
CCCCCCCEEECCEEEEHHHHHHHCCCEEECCCCHHCCCCCCCCCCCCCCCCCCCCCCHHH
LHTDHLEAHFVPRPKVYWDPVAAVISYDGQLATAGITTVLDSLRVWSEEGAEGVDGQANV
HHHHHHHEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LADAIARARDARLLRADHFLHLRCEVPMPTVVEEAKQLAGRSDVRLMSLMDHTPGQRQFR
HHHHHHHHHHHHHEECCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHH
DEGKLRDYYRGKSGGKTDAELDVMFARRFAYQQAYAAANMRDIVALAKTHDVPLASHDDT
HCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
TDENVADAIKDGIAVAEFPTTMEAARGLHEAGISILMGAPNVVRGGSHSGNIAAVDLARE
CCHHHHHHHHCCCEEECCCCHHHHHCCHHHCCCEEEECCCCCCCCCCCCCCEEEEHHHHH
GLLDILSSDYVPSSLLMGALQLPRRVPAIDLAAAVRTVTKAPADAVGLRDRGEIAPGKRA
HHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCC
DLIRVHVAGDHAVVRSVWREGLRVA
CEEEEEECCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SANMSDTVIGNARLVLADRVIEQGWVALAGGRIAEIGEGAAPAGSHDAGGDLLMPGLVE
CCCCCCEEECCEEEEHHHHHHHCCCEEECCCCHHCCCCCCCCCCCCCCCCCCCCCCHHH
LHTDHLEAHFVPRPKVYWDPVAAVISYDGQLATAGITTVLDSLRVWSEEGAEGVDGQANV
HHHHHHHEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LADAIARARDARLLRADHFLHLRCEVPMPTVVEEAKQLAGRSDVRLMSLMDHTPGQRQFR
HHHHHHHHHHHHHEECCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHH
DEGKLRDYYRGKSGGKTDAELDVMFARRFAYQQAYAAANMRDIVALAKTHDVPLASHDDT
HCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
TDENVADAIKDGIAVAEFPTTMEAARGLHEAGISILMGAPNVVRGGSHSGNIAAVDLARE
CCHHHHHHHHCCCEEECCCCHHHHHCCHHHCCCEEEECCCCCCCCCCCCCCEEEEHHHHH
GLLDILSSDYVPSSLLMGALQLPRRVPAIDLAAAVRTVTKAPADAVGLRDRGEIAPGKRA
HHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCC
DLIRVHVAGDHAVVRSVWREGLRVA
CEEEEEECCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]