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The map label for this gene is minD [C]

Identifier: 146342080

GI number: 146342080

Start: 5435985

End: 5437253

Strand: Direct

Name: minD [C]

Synonym: BRADO5221

Alternate gene names: 146342080

Gene position: 5435985-5437253 (Clockwise)

Preceding gene: 146342079

Following gene: 146342084

Centisome position: 72.9

GC content: 67.22

Gene sequence:

>1269_bases
ATGATCAACGCGTCCCGTCACAGCCAGCATGAGCAGCCGGCGCCGAGCGGCCCGGTGCTCGACGATCACATCGCGCCGGT
TCCCCGCGTCTCGGTGCAGGCGTTCACCGAGACCGACCAGACCTCGTCCGCGGTGCTCGCGGCGAGCCAGGACCGGAGGC
TCGGCAAGGCGCACATCACGATCAAGGCGGGCGGCATGGCCCGCGCCATCGAGACCTATCATTCGCAGCCGACGCCCAAC
GTCATCGTCATCGAGACCAAGCCGGGCGGCGACATCATCGGCGGCCTCGACGATCTCGCCAGCGTCTGCGATCCCGGCAC
ACGCGTGGTGCTGATCGGAAGCCCGAGCGATGGCGTTGCCTATCGCGAGCTGGTGCGCCGCGGCGTCAACGACTACGTGA
TCGGCCCGGTCGAGACCATCGACGTGATCCGCTCGATCTGCAGCCTGTTCTCGGCCTCGGAGGCCGTCATCACCGGCCGC
ATCATCGCGGTGGTCGGCGCCAAGGGCGGCGTCGGCGCCTCCACGGTGGCGCACAACGTCGCCTGGAGCATGGCGCGCGA
CCTGACGCTGGATTCCGTGGTGATCGATCTCGACATGGCGTTCGGAACCGCCGGCCTTGACTACAACCAGGATCCGATGC
AGGGCATCGCCAACGCCATCTTCCAGCCGGACCGGCCGGACACGGCGCTGATGGAGCGCCTGCTCGCCAAATGCACCGAG
CACCTCAATCTGCTCGCAGCCCCCGCGACGCTGGACCGCGTCTATGATTTCGGCGCCGACGCGTTCGACGCGATCTTCGA
CACGCTGCGGCTAACGCAGCAATGCATCGTGCTCGACGTGCCGCACCAATGGTCGGCCTGGACGCGGCATGCGCTGATCA
ACGCCGATGACATCCTCGTCGTCGCCGAACCGGATCTCGCCAATCTGCGCAACGCCAAGAACCTCTTGAGCGTGCTGAAA
TCCTCGCGCCCCAACGACCGCCCGCCGCTGTACTGCCTGAACCAGGTCGGCATGCCCAAGCGGCCGGAGATCGATTTGAA
GCGCTTCGCCAAGACGGTGGAGGCCGAGCCGGTCGCGGTGATCCCGTTCGACTGCAAGCTGTTCGGCACCGCCGCCAACA
ACGGCCAGATGATCGCGGAGGTCGGTGCGCTCAGCCGCACGCCGAAGCTGTTCAAGGAGCTCGCGAACCGGCTGACCGGA
CGCGTCGAGCCGAAGCCGCAGCGCGGCTCGCTGCTGGCGCCGATCGCAAAGCTGCTCGGCGCGAAATAG

Upstream 100 bases:

>100_bases
CGCCGCGCCGCTCCGCTCTGTTCGAGAAGTACCGCAAGGGCGAGTCGACGGCCGTGACCTATCCGGAGACCGAGAAGGCC
AAGCTGAGCGATACAGGCAA

Downstream 100 bases:

>100_bases
CGCTCAGTCGTCGGAGCGGCTCAGCGCTTTTGCCAGCGCCTTGTCCGGCTGGCCCGCGACGGGCCGGTCCTGCCGCGGCG
ACAGCTCGCGCTGCGCGACC

Product: putative pilus assembly protein CpaE

Products: NA

Alternate protein names: Pilus Assembly Protein CpaE; Pilus Assembly Protein; Pilus Assembly ATPase CpaE; Response Regulator Receiver; Flp Pilus Assembly Protein ATPase CpaE; Flp Pilus Assembly Protein ATPase CpaE-Like Protein; Response Regulator Receiver Domain-Containing Protein; Two-Component Response Regulator; CtpF Protein; CpaE2 Pilus Assembly Protein; TadE-Like; Component Of Type IV Pilus; Components Of Type IV Pilus

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MINASRHSQHEQPAPSGPVLDDHIAPVPRVSVQAFTETDQTSSAVLAASQDRRLGKAHITIKAGGMARAIETYHSQPTPN
VIVIETKPGGDIIGGLDDLASVCDPGTRVVLIGSPSDGVAYRELVRRGVNDYVIGPVETIDVIRSICSLFSASEAVITGR
IIAVVGAKGGVGASTVAHNVAWSMARDLTLDSVVIDLDMAFGTAGLDYNQDPMQGIANAIFQPDRPDTALMERLLAKCTE
HLNLLAAPATLDRVYDFGADAFDAIFDTLRLTQQCIVLDVPHQWSAWTRHALINADDILVVAEPDLANLRNAKNLLSVLK
SSRPNDRPPLYCLNQVGMPKRPEIDLKRFAKTVEAEPVAVIPFDCKLFGTAANNGQMIAEVGALSRTPKLFKELANRLTG
RVEPKPQRGSLLAPIAKLLGAK

Sequences:

>Translated_422_residues
MINASRHSQHEQPAPSGPVLDDHIAPVPRVSVQAFTETDQTSSAVLAASQDRRLGKAHITIKAGGMARAIETYHSQPTPN
VIVIETKPGGDIIGGLDDLASVCDPGTRVVLIGSPSDGVAYRELVRRGVNDYVIGPVETIDVIRSICSLFSASEAVITGR
IIAVVGAKGGVGASTVAHNVAWSMARDLTLDSVVIDLDMAFGTAGLDYNQDPMQGIANAIFQPDRPDTALMERLLAKCTE
HLNLLAAPATLDRVYDFGADAFDAIFDTLRLTQQCIVLDVPHQWSAWTRHALINADDILVVAEPDLANLRNAKNLLSVLK
SSRPNDRPPLYCLNQVGMPKRPEIDLKRFAKTVEAEPVAVIPFDCKLFGTAANNGQMIAEVGALSRTPKLFKELANRLTG
RVEPKPQRGSLLAPIAKLLGAK
>Mature_422_residues
MINASRHSQHEQPAPSGPVLDDHIAPVPRVSVQAFTETDQTSSAVLAASQDRRLGKAHITIKAGGMARAIETYHSQPTPN
VIVIETKPGGDIIGGLDDLASVCDPGTRVVLIGSPSDGVAYRELVRRGVNDYVIGPVETIDVIRSICSLFSASEAVITGR
IIAVVGAKGGVGASTVAHNVAWSMARDLTLDSVVIDLDMAFGTAGLDYNQDPMQGIANAIFQPDRPDTALMERLLAKCTE
HLNLLAAPATLDRVYDFGADAFDAIFDTLRLTQQCIVLDVPHQWSAWTRHALINADDILVVAEPDLANLRNAKNLLSVLK
SSRPNDRPPLYCLNQVGMPKRPEIDLKRFAKTVEAEPVAVIPFDCKLFGTAANNGQMIAEVGALSRTPKLFKELANRLTG
RVEPKPQRGSLLAPIAKLLGAK

Specific function: ATPase Required For The Correct Placement Of The Division Site. Cell Division Inhibitors Minc And Mind Act In Concert To Form An Inhibitor Capable Of Blocking Formation Of The Polar Z Ring Septums. Rapidly Oscillates Between The Poles Of The Cell To Dest

COG id: COG4963

COG function: function code U; Flp pilus assembly protein, ATPase CpaE

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45290; Mature: 45290

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINASRHSQHEQPAPSGPVLDDHIAPVPRVSVQAFTETDQTSSAVLAASQDRRLGKAHIT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEEE
IKAGGMARAIETYHSQPTPNVIVIETKPGGDIIGGLDDLASVCDPGTRVVLIGSPSDGVA
EECCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHH
YRELVRRGVNDYVIGPVETIDVIRSICSLFSASEAVITGRIIAVVGAKGGVGASTVAHNV
HHHHHHCCCCCEEECCHHHHHHHHHHHHHHCCCCHHHEEEEEEEEECCCCCCHHHHHHHH
AWSMARDLTLDSVVIDLDMAFGTAGLDYNQDPMQGIANAIFQPDRPDTALMERLLAKCTE
HHHHHHCCCHHHEEEEEEHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
HLNLLAAPATLDRVYDFGADAFDAIFDTLRLTQQCIVLDVPHQWSAWTRHALINADDILV
HHHEEECCHHHHHHHHCCCHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHEECCCCEEE
VAEPDLANLRNAKNLLSVLKSSRPNDRPPLYCLNQVGMPKRPEIDLKRFAKTVEAEPVAV
EECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEHHCCCCCCCCCHHHHHHHHHHCCCCEEE
IPFDCKLFGTAANNGQMIAEVGALSRTPKLFKELANRLTGRVEPKPQRGSLLAPIAKLLG
EEECCEEEEEECCCCCEEEHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
AK
CC
>Mature Secondary Structure
MINASRHSQHEQPAPSGPVLDDHIAPVPRVSVQAFTETDQTSSAVLAASQDRRLGKAHIT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEEE
IKAGGMARAIETYHSQPTPNVIVIETKPGGDIIGGLDDLASVCDPGTRVVLIGSPSDGVA
EECCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHH
YRELVRRGVNDYVIGPVETIDVIRSICSLFSASEAVITGRIIAVVGAKGGVGASTVAHNV
HHHHHHCCCCCEEECCHHHHHHHHHHHHHHCCCCHHHEEEEEEEEECCCCCCHHHHHHHH
AWSMARDLTLDSVVIDLDMAFGTAGLDYNQDPMQGIANAIFQPDRPDTALMERLLAKCTE
HHHHHHCCCHHHEEEEEEHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
HLNLLAAPATLDRVYDFGADAFDAIFDTLRLTQQCIVLDVPHQWSAWTRHALINADDILV
HHHEEECCHHHHHHHHCCCHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHEECCCCEEE
VAEPDLANLRNAKNLLSVLKSSRPNDRPPLYCLNQVGMPKRPEIDLKRFAKTVEAEPVAV
EECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEHHCCCCCCCCCHHHHHHHHHHCCCCEEE
IPFDCKLFGTAANNGQMIAEVGALSRTPKLFKELANRLTGRVEPKPQRGSLLAPIAKLLG
EEECCEEEEEECCCCCEEEHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
AK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA