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The map label for this gene is rfaF [H]

Identifier: 146342063

GI number: 146342063

Start: 5416356

End: 5417420

Strand: Direct

Name: rfaF [H]

Synonym: BRADO5203

Alternate gene names: 146342063

Gene position: 5416356-5417420 (Clockwise)

Preceding gene: 146342058

Following gene: 146342064

Centisome position: 72.64

GC content: 70.99

Gene sequence:

>1065_bases
ATGAATTCCAATTCGGCCAACATCGCGGTGGACGCTGACGACACCAGCCCGATCCTGATCATTCCCTATATGTGGATCGG
CGATTTCGTCCGCGGCCACACCGTGGTGCGGGTGCTCAAGGAGCGCTGGCCGAATCGGCCCGTGGACCTGCTGGTGACCT
CGTTGTGCGCGCCGCTGGTCGATTACATGCCCGGCGTCCGCTCCGGCATCGTCTGGGACCTGCCGCGCAGCCGGCTGGCG
CTGGCCAGGCAGTGGCATCTCGCCAGCCTGTTGCGCGAGCGCCACTATGGTACCGCCCTGGTGATGCCGCGCACGTGGAA
GTCCGCCTTGGCGCCGGCGCTTGCCGCCATTCCCGAACGCGTCGGCTTCGTCGGCGAGATCAGGTTCGGCCTGCTGAACC
GCTGGCGCTGGGGCGAGAAGGCGCTGCCCCGCTTCATCGACAAGAACGCGGCCCTCGCTTTGCCCGACGGCGCGCCGCTG
CCGCCGGAATGGCCGGTGCCGCAGCTCCGGGTGCCGCCGGGAGATGTCGCGCGCTGGCGCGAGGCGAACGGCCTGGGGAG
TCGGCCCGCGGTCGCGCTGGCGCCGGGGTCGGTCGGCGCCGCGAAGCGCTGGCCCTTCTACGGCGAGGCAGCCCGACTGC
TGCACGCGGCGGGCCTCGATGTCTGGGTGGTCGGCGGTCCCGGCGAGAAGGCGCTCGCCGCCGAGATCGTCGCCACAGGC
GGCCCGGAGGTCCGCGACCTCACCGGAACCGACCTGCGCAACGGCATCCTGGCGATGGCGGCGGCCGGTGTCGCCATCTC
CAACGATTCGGGCCTGATGCACATCGCCGCCGCCCTGGGCACGCCGACCATGGGTATCTTCGGCCCGACCAGCCCGTACC
ATTGGGCGCCGCTGAACGGCCTCGCCGCGACCGTGCAGACAAAGACGCAGGTGCCCTGCCAGCCTTGCCATCGTCCGGTC
TGCCGGATGAACGATCACCGCTGCATGCGGGACATCCCGGCCATCGATGTGGCCGCGATCGCCCAGCGCGTGCTGGCTGA
GGCGGCGATCCCGGTCGCCCCCTAG

Upstream 100 bases:

>100_bases
GCGGCGACAAAGTGTAAGCGCTTTGGTGACGGCCGAGACCGGCCACTTTACCTGTGGCGGCTGAGCGGCTACCAGCGCGC
CGGAGCCAGGCAGACAAAGA

Downstream 100 bases:

>100_bases
CTTGCGCGGGGACGGCGCGCCCCCTACACCGGCCCTATCGCAATCCGAGCCGCCATCGTGACCGACGCACTGGACAGCCT
GATCTCCAACCATCTCGCCC

Product: ADP-heptose--LPS heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MNSNSANIAVDADDTSPILIIPYMWIGDFVRGHTVVRVLKERWPNRPVDLLVTSLCAPLVDYMPGVRSGIVWDLPRSRLA
LARQWHLASLLRERHYGTALVMPRTWKSALAPALAAIPERVGFVGEIRFGLLNRWRWGEKALPRFIDKNAALALPDGAPL
PPEWPVPQLRVPPGDVARWREANGLGSRPAVALAPGSVGAAKRWPFYGEAARLLHAAGLDVWVVGGPGEKALAAEIVATG
GPEVRDLTGTDLRNGILAMAAAGVAISNDSGLMHIAAALGTPTMGIFGPTSPYHWAPLNGLAATVQTKTQVPCQPCHRPV
CRMNDHRCMRDIPAIDVAAIAQRVLAEAAIPVAP

Sequences:

>Translated_354_residues
MNSNSANIAVDADDTSPILIIPYMWIGDFVRGHTVVRVLKERWPNRPVDLLVTSLCAPLVDYMPGVRSGIVWDLPRSRLA
LARQWHLASLLRERHYGTALVMPRTWKSALAPALAAIPERVGFVGEIRFGLLNRWRWGEKALPRFIDKNAALALPDGAPL
PPEWPVPQLRVPPGDVARWREANGLGSRPAVALAPGSVGAAKRWPFYGEAARLLHAAGLDVWVVGGPGEKALAAEIVATG
GPEVRDLTGTDLRNGILAMAAAGVAISNDSGLMHIAAALGTPTMGIFGPTSPYHWAPLNGLAATVQTKTQVPCQPCHRPV
CRMNDHRCMRDIPAIDVAAIAQRVLAEAAIPVAP
>Mature_354_residues
MNSNSANIAVDADDTSPILIIPYMWIGDFVRGHTVVRVLKERWPNRPVDLLVTSLCAPLVDYMPGVRSGIVWDLPRSRLA
LARQWHLASLLRERHYGTALVMPRTWKSALAPALAAIPERVGFVGEIRFGLLNRWRWGEKALPRFIDKNAALALPDGAPL
PPEWPVPQLRVPPGDVARWREANGLGSRPAVALAPGSVGAAKRWPFYGEAARLLHAAGLDVWVVGGPGEKALAAEIVATG
GPEVRDLTGTDLRNGILAMAAAGVAISNDSGLMHIAAALGTPTMGIFGPTSPYHWAPLNGLAATVQTKTQVPCQPCHRPV
CRMNDHRCMRDIPAIDVAAIAQRVLAEAAIPVAP

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=309, Percent_Identity=35.9223300970874, Blast_Score=163, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 38044; Mature: 38044

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSNSANIAVDADDTSPILIIPYMWIGDFVRGHTVVRVLKERWPNRPVDLLVTSLCAPLV
CCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
DYMPGVRSGIVWDLPRSRLALARQWHLASLLRERHYGTALVMPRTWKSALAPALAAIPER
HHCCCHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHH
VGFVGEIRFGLLNRWRWGEKALPRFIDKNAALALPDGAPLPPEWPVPQLRVPPGDVARWR
HCHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEECCCCHHHHHH
EANGLGSRPAVALAPGSVGAAKRWPFYGEAARLLHAAGLDVWVVGGPGEKALAAEIVATG
HCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCC
GPEVRDLTGTDLRNGILAMAAAGVAISNDSGLMHIAAALGTPTMGIFGPTSPYHWAPLNG
CCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCC
LAATVQTKTQVPCQPCHRPVCRMNDHRCMRDIPAIDVAAIAQRVLAEAAIPVAP
CEEEEECCCCCCCHHHCCCHHCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNSNSANIAVDADDTSPILIIPYMWIGDFVRGHTVVRVLKERWPNRPVDLLVTSLCAPLV
CCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
DYMPGVRSGIVWDLPRSRLALARQWHLASLLRERHYGTALVMPRTWKSALAPALAAIPER
HHCCCHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHH
VGFVGEIRFGLLNRWRWGEKALPRFIDKNAALALPDGAPLPPEWPVPQLRVPPGDVARWR
HCHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEECCCCHHHHHH
EANGLGSRPAVALAPGSVGAAKRWPFYGEAARLLHAAGLDVWVVGGPGEKALAAEIVATG
HCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCC
GPEVRDLTGTDLRNGILAMAAAGVAISNDSGLMHIAAALGTPTMGIFGPTSPYHWAPLNG
CCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCC
LAATVQTKTQVPCQPCHRPVCRMNDHRCMRDIPAIDVAAIAQRVLAEAAIPVAP
CEEEEECCCCCCCHHHCCCHHCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]