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The map label for this gene is asnB [H]

Identifier: 146342019

GI number: 146342019

Start: 5361472

End: 5363442

Strand: Reverse

Name: asnB [H]

Synonym: BRADO5156

Alternate gene names: 146342019

Gene position: 5363442-5361472 (Counterclockwise)

Preceding gene: 146342023

Following gene: 146342017

Centisome position: 71.93

GC content: 66.31

Gene sequence:

>1971_bases
ATGTGTGGCATCGCGGGCTTGCTGCAGACCGAGCGGGCGACGGATCATGCCGGGTTGGTCTCGATCACCGATTGCATGAC
CGCGGCCTTGGCGCACCGTGGCCCGGATGCATCGGGCACATGGATAGACGCGCGGCATGGGGTCTGCTTTGGTCAGCGAC
GCCTCGCGATCCTCGATCTGTCCGAGGCTGGCGCGCAGCCGATGCATGACGCCTGCGGGCGCTACACGATCACCTTCAAT
GGCGAGATCTACAATCATCTGGACATAAGGGCCGAGCTGGATGCGGGTGGCGCCGCCCCGAATTGGCGCGGTCATTCCGA
TACCGAGACGCTGCTCGCCGCGATCAGGCAGTGGGGTGTGAATCGGGCGCTGCAGCGGCTGATCGGCATGTTTGCGTTCG
CGCTGTGGGACGCAGAGGCGCGTGAGCTTGTGCTGGCGCGTGACCGTTTCGGCGAGAAGCCACTGTTCTATGGCTGGATC
GGTGGCGACCTGGTGTTCGGATCGGAGCTCAAGGCGTTGGCCGCGCATCCGTCCTGGTCCCCGTCGCTGGATCGCGCGGC
GCTCACGGCGTTCATGCGCTACTCCTACGTTCCGGCACCGGCCACAATCTGGGGTGGCGTTCGTAAGCTTCCGCCCGCAT
CGTTCGTAAGCTTTCCCTCTGACGCCGTGCCGGGCGAGTTGCCCGAGCCGCGACCCTATTGGTCGCTGCGCGATCGGCTG
ACCTCAGGCCGGGGCGACCGGATCGACGGCGAAAGCGAAGCGATCGATCGGTTAGGGCAGCTTCTTTCGACCGCCGTCAA
GCGGCAGTGCCTTTCGGACGTGCCGCTCGGCGCGTTTCTCTCCGGCGGAATCGATTCATCGACGATTGTCGCCTTGATGC
AGGCGCAGGCCAGCCAGCCGGTGCGCACCTTCAGCATCGGCTTCGCCGAGGGCGGCTACAACGAGGCCGAGGATGCGCGC
CGCGTCGCGCGGCATCTCGGCACCGATCATACCGAGATGTATGTCGACGCGCGCACTGCGATGGACGTGATCCCGAAGCT
GCCGCGCATCTATGACGAGCCGTTCGCCGACTCCTCGCAGATCCCAACGCATCTCGTGGCCCAACTCGCCCGCCGGCACG
TCACGGTCGCGCTGTCCGGCGATGCCGGGGACGAGTTGTTCGGCGGCTACAACAGGCACGTTTGGGGCGGCGCCCTGCAA
GCGCGTCTCGGCCGTTGGCCGCTGCCGCTGCGGCGTGCTCTCGGGGCCGTGCTCGGCGCTATCGCTCCGGAACCGGCCGA
TACCGTTCTCGGGGCGTTGCAGCCGCTGCTGCCGCGCCGGTTCAAGGTCCGGCATCCCGGAGATCAGGTTGCCAAGCTTG
GACGTCTCATCGCGGCCGATGGATTCGACGGTTTGTACCGAGCGCTGTGCTCGATCGATCCGTCTCCGACGCGCACGGTC
TTGGACGGGGAGGAGCGCGCGCACTGGGCCGGCGAGCAGATGCGGCAAGGCGCAGCGGCTCTCGATCCGCTCGACCGCAT
GACGCTGGCCGATTCGCTCAGCTATCTCAGCGACGACATTCTGCAGAAGGTGGACCGTGCGGCCATGGCGGTGGCGCTGG
AGACGCGGGTGCCGTTCCTCGACAAGGACGTCGTCGAATTCGCAGCGCGGGTTCCGCCCGATATGAAGGTGCGCGACGGC
CGCGGCAAATGGCTGGTGAGGCAGGTGCTTTACCGGTATGTGCCGGCCGAAATGATCGACCGGCCGAAGACCGGCTTCAG
CATACCCCTGGACTCGTGGCTGCGCGGGCCGCTGAAATCATGGGCCTCAGACCTGCTGTCGCCGGAGCGGCTGAAGCGGC
AGGGTCTGTTCGATCCGGTGCGGGTGACGCGCATGTTGGACGAGCATCTCAGCCGACGGCACAATCATGCCTACTGGCTG
TGGAACGTGCTGATGGCCGAAGCCTGGTACGACGAATGGGCGCGGGCTTGA

Upstream 100 bases:

>100_bases
AAGAGAAGCTTCGAGAGAAAGATTGCGTGTTGTCGGCTGGGTCCATCGGCGTGTATAGCGCATCGGGTAAAATTCTGGGG
CAGGTTGGAAGATCTGGCAT

Downstream 100 bases:

>100_bases
GGTCGCTCAGTCCTCGACGGTAGCGGGGACCGCCAGCTTGCCCTGGCCCTTCTCGGCGCTGAGAATCCAGACGATGCCGC
CCATGCCGCCAACGATGAAG

Product: putative asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MCGIAGLLQTERATDHAGLVSITDCMTAALAHRGPDASGTWIDARHGVCFGQRRLAILDLSEAGAQPMHDACGRYTITFN
GEIYNHLDIRAELDAGGAAPNWRGHSDTETLLAAIRQWGVNRALQRLIGMFAFALWDAEARELVLARDRFGEKPLFYGWI
GGDLVFGSELKALAAHPSWSPSLDRAALTAFMRYSYVPAPATIWGGVRKLPPASFVSFPSDAVPGELPEPRPYWSLRDRL
TSGRGDRIDGESEAIDRLGQLLSTAVKRQCLSDVPLGAFLSGGIDSSTIVALMQAQASQPVRTFSIGFAEGGYNEAEDAR
RVARHLGTDHTEMYVDARTAMDVIPKLPRIYDEPFADSSQIPTHLVAQLARRHVTVALSGDAGDELFGGYNRHVWGGALQ
ARLGRWPLPLRRALGAVLGAIAPEPADTVLGALQPLLPRRFKVRHPGDQVAKLGRLIAADGFDGLYRALCSIDPSPTRTV
LDGEERAHWAGEQMRQGAAALDPLDRMTLADSLSYLSDDILQKVDRAAMAVALETRVPFLDKDVVEFAARVPPDMKVRDG
RGKWLVRQVLYRYVPAEMIDRPKTGFSIPLDSWLRGPLKSWASDLLSPERLKRQGLFDPVRVTRMLDEHLSRRHNHAYWL
WNVLMAEAWYDEWARA

Sequences:

>Translated_656_residues
MCGIAGLLQTERATDHAGLVSITDCMTAALAHRGPDASGTWIDARHGVCFGQRRLAILDLSEAGAQPMHDACGRYTITFN
GEIYNHLDIRAELDAGGAAPNWRGHSDTETLLAAIRQWGVNRALQRLIGMFAFALWDAEARELVLARDRFGEKPLFYGWI
GGDLVFGSELKALAAHPSWSPSLDRAALTAFMRYSYVPAPATIWGGVRKLPPASFVSFPSDAVPGELPEPRPYWSLRDRL
TSGRGDRIDGESEAIDRLGQLLSTAVKRQCLSDVPLGAFLSGGIDSSTIVALMQAQASQPVRTFSIGFAEGGYNEAEDAR
RVARHLGTDHTEMYVDARTAMDVIPKLPRIYDEPFADSSQIPTHLVAQLARRHVTVALSGDAGDELFGGYNRHVWGGALQ
ARLGRWPLPLRRALGAVLGAIAPEPADTVLGALQPLLPRRFKVRHPGDQVAKLGRLIAADGFDGLYRALCSIDPSPTRTV
LDGEERAHWAGEQMRQGAAALDPLDRMTLADSLSYLSDDILQKVDRAAMAVALETRVPFLDKDVVEFAARVPPDMKVRDG
RGKWLVRQVLYRYVPAEMIDRPKTGFSIPLDSWLRGPLKSWASDLLSPERLKRQGLFDPVRVTRMLDEHLSRRHNHAYWL
WNVLMAEAWYDEWARA
>Mature_656_residues
MCGIAGLLQTERATDHAGLVSITDCMTAALAHRGPDASGTWIDARHGVCFGQRRLAILDLSEAGAQPMHDACGRYTITFN
GEIYNHLDIRAELDAGGAAPNWRGHSDTETLLAAIRQWGVNRALQRLIGMFAFALWDAEARELVLARDRFGEKPLFYGWI
GGDLVFGSELKALAAHPSWSPSLDRAALTAFMRYSYVPAPATIWGGVRKLPPASFVSFPSDAVPGELPEPRPYWSLRDRL
TSGRGDRIDGESEAIDRLGQLLSTAVKRQCLSDVPLGAFLSGGIDSSTIVALMQAQASQPVRTFSIGFAEGGYNEAEDAR
RVARHLGTDHTEMYVDARTAMDVIPKLPRIYDEPFADSSQIPTHLVAQLARRHVTVALSGDAGDELFGGYNRHVWGGALQ
ARLGRWPLPLRRALGAVLGAIAPEPADTVLGALQPLLPRRFKVRHPGDQVAKLGRLIAADGFDGLYRALCSIDPSPTRTV
LDGEERAHWAGEQMRQGAAALDPLDRMTLADSLSYLSDDILQKVDRAAMAVALETRVPFLDKDVVEFAARVPPDMKVRDG
RGKWLVRQVLYRYVPAEMIDRPKTGFSIPLDSWLRGPLKSWASDLLSPERLKRQGLFDPVRVTRMLDEHLSRRHNHAYWL
WNVLMAEAWYDEWARA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=411, Percent_Identity=28.7104622871046, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI168229252, Length=411, Percent_Identity=28.7104622871046, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI168229250, Length=411, Percent_Identity=28.7104622871046, Blast_Score=127, Evalue=4e-29,
Organism=Escherichia coli, GI1786889, Length=409, Percent_Identity=31.2958435207824, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71993933, Length=403, Percent_Identity=26.5508684863524, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI25147557, Length=397, Percent_Identity=27.2040302267003, Blast_Score=119, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI25147560, Length=369, Percent_Identity=26.0162601626016, Blast_Score=103, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6325403, Length=405, Percent_Identity=27.6543209876543, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321563, Length=429, Percent_Identity=25.4079254079254, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45553209, Length=370, Percent_Identity=29.1891891891892, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72427; Mature: 72427

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGLLQTERATDHAGLVSITDCMTAALAHRGPDASGTWIDARHGVCFGQRRLAILDL
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCEECCCCEEEEEC
SEAGAQPMHDACGRYTITFNGEIYNHLDIRAELDAGGAAPNWRGHSDTETLLAAIRQWGV
HHHCCCCHHHHCCCEEEEECCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
NRALQRLIGMFAFALWDAEARELVLARDRFGEKPLFYGWIGGDLVFGSELKALAAHPSWS
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEEECCCEEECCHHHHHHCCCCCC
PSLDRAALTAFMRYSYVPAPATIWGGVRKLPPASFVSFPSDAVPGELPEPRPYWSLRDRL
CCHHHHHHHHHHHHHCCCCCHHHHCCHHCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHH
TSGRGDRIDGESEAIDRLGQLLSTAVKRQCLSDVPLGAFLSGGIDSSTIVALMQAQASQP
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCC
VRTFSIGFAEGGYNEAEDARRVARHLGTDHTEMYVDARTAMDVIPKLPRIYDEPFADSSQ
CEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHEEEEHHHHHHHHHHHHHHHCCCCCCCCC
IPTHLVAQLARRHVTVALSGDAGDELFGGYNRHVWGGALQARLGRWPLPLRRALGAVLGA
CHHHHHHHHHHCCEEEEECCCCCHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
IAPEPADTVLGALQPLLPRRFKVRHPGDQVAKLGRLIAADGFDGLYRALCSIDPSPTRTV
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEE
LDGEERAHWAGEQMRQGAAALDPLDRMTLADSLSYLSDDILQKVDRAAMAVALETRVPFL
ECCHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DKDVVEFAARVPPDMKVRDGRGKWLVRQVLYRYVPAEMIDRPKTGFSIPLDSWLRGPLKS
CHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHH
WASDLLSPERLKRQGLFDPVRVTRMLDEHLSRRHNHAYWLWNVLMAEAWYDEWARA
HHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCGIAGLLQTERATDHAGLVSITDCMTAALAHRGPDASGTWIDARHGVCFGQRRLAILDL
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCEECCCCEEEEEC
SEAGAQPMHDACGRYTITFNGEIYNHLDIRAELDAGGAAPNWRGHSDTETLLAAIRQWGV
HHHCCCCHHHHCCCEEEEECCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
NRALQRLIGMFAFALWDAEARELVLARDRFGEKPLFYGWIGGDLVFGSELKALAAHPSWS
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEEECCCEEECCHHHHHHCCCCCC
PSLDRAALTAFMRYSYVPAPATIWGGVRKLPPASFVSFPSDAVPGELPEPRPYWSLRDRL
CCHHHHHHHHHHHHHCCCCCHHHHCCHHCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHH
TSGRGDRIDGESEAIDRLGQLLSTAVKRQCLSDVPLGAFLSGGIDSSTIVALMQAQASQP
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCC
VRTFSIGFAEGGYNEAEDARRVARHLGTDHTEMYVDARTAMDVIPKLPRIYDEPFADSSQ
CEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHEEEEHHHHHHHHHHHHHHHCCCCCCCCC
IPTHLVAQLARRHVTVALSGDAGDELFGGYNRHVWGGALQARLGRWPLPLRRALGAVLGA
CHHHHHHHHHHCCEEEEECCCCCHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
IAPEPADTVLGALQPLLPRRFKVRHPGDQVAKLGRLIAADGFDGLYRALCSIDPSPTRTV
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEE
LDGEERAHWAGEQMRQGAAALDPLDRMTLADSLSYLSDDILQKVDRAAMAVALETRVPFL
ECCHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DKDVVEFAARVPPDMKVRDGRGKWLVRQVLYRYVPAEMIDRPKTGFSIPLDSWLRGPLKS
CHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHH
WASDLLSPERLKRQGLFDPVRVTRMLDEHLSRRHNHAYWLWNVLMAEAWYDEWARA
HHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]