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The map label for this gene is capL [H]

Identifier: 146342017

GI number: 146342017

Start: 5359006

End: 5360319

Strand: Reverse

Name: capL [H]

Synonym: BRADO5154

Alternate gene names: 146342017

Gene position: 5360319-5359006 (Counterclockwise)

Preceding gene: 146342019

Following gene: 146342016

Centisome position: 71.89

GC content: 64.84

Gene sequence:

>1314_bases
ATGCCTCCGAAACGACCGGAATATCAATTTATGTCTCATGGCCGGAAGATTGCGGTGATAGGCCTTGGTTACGTCGGCCT
GCCGGTCGCCGCGGCGTTCGCGCGCGCCGGCTGCCCGGTGATCGGATTCGACATCGATGCTGCCAGGGTCGCCGAGCTGA
GGGACGGCAGGGACCGCACCTGTGAGGTCGAGGCCGCCGATCTTAAGCGGCCCGGCCTGCGCTTCACCAGCGATCCGGCT
GACATGGCTGCGGCCGATTTCTTCATTGTCACCGTCCCGACCCCGATCGACAGTGCTCGCCGGCCCGATTTGAGCGCAAT
GCGCTCCGCCACGCGCACCGTGAGGGCGGTGCTGAAGCGCGGCGACATCGTGGTCTATGAGTCGACGGTGTATCCTGGCG
CGGTCGAGGAGGACTGTGTTCCGCTGCTGGAGAGCGGCTCCGAGCTGAAGGCCGGCCTTGATTTCAACGTCGGCTACTCG
CCGGAGCGCATCAATCCGGGCGACAAGACGCATCGCTTCGAGACGATCACCAAGGTCGTCTCGGCACAGAATCCGCAGAC
GCTGCAGATCGTCGCCGAGGTCTACGGATCAGTGGTCAAAGCCGGTATTCATCGCGCGCCCTCGATCAGGGTTGCCGAGG
CTGCGAAGGTGATCGAGAACACGCAACGCGACCTCAACATCGCCTTCATGAACGAGCTCTCGCTGATCTTCCAGGCGCTG
GAGATCGACACCGGCGACGTGCTCGCGGCGGCCGCGACCAAGTGGAACTTCCTGCCGTTCACGCCGGGCCTCGTCGGCGG
ACACTGCATCGGCGTCGATCCCTATTATCTCACTTATCGCGCCGAGAAGGCGGGCTATCATCCCGAGGTGATCCTGGCGG
GCCGTCGGATCAATGACGAGATGGGCCGGCGCATCGCGCGCGAATGCATCCGCGGACTCCTGAAGCGGAAGGGGCGCAGC
GGCGTCGTGACCATTCTCGGCATGACGTTCAAGGAGAACGTGCCCGACATTCGCAATTCGCGGGTGATCGACATCATCAG
TGAGTTGCAGTCGTTTGGTATCGACGTTCAGGTGGCCGATCCCCTGGCCGATGCCGCGGCCGTGCACGAGGAGTATGGGA
TTGCCCTGACGGCGGCCGAGGCGCTGCGCCCGGCCGACGCGGTCGTGCTCGCCGTGTCGCATGATCAGTTCCTCGCCGGC
GGCTGGCCGCTGGTGCAGGGGCTGCTGCGCGAGGGGAGCGGCCTCGTACTCGATGTCAAGATGAAGCTCGACCGCGCGAG
CAAACCCGGCGCCATCGAGCTGTGGCGGCCCTGA

Upstream 100 bases:

>100_bases
CTGGCGGACCGGACCTCAGACGAGGCCCCGCGCCGTGGCGGCGGCCGACCCCTTGCGGTCCTGACGCCCCGGTGGCTAAA
CAGTCGCAGGGGTGGCGTCA

Downstream 100 bases:

>100_bases
CGGAGCGACCATGGCAAACGAACCCATCCTCGTCACCGGCGCCGCCGGCTTCATCGGGTTTCATCTGACCCAGCAACTGC
TCGCTGAGGGGCGGCAGGTC

Product: UDP-glucose/GDP-mannose dehydrogenase family protein

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MPPKRPEYQFMSHGRKIAVIGLGYVGLPVAAAFARAGCPVIGFDIDAARVAELRDGRDRTCEVEAADLKRPGLRFTSDPA
DMAAADFFIVTVPTPIDSARRPDLSAMRSATRTVRAVLKRGDIVVYESTVYPGAVEEDCVPLLESGSELKAGLDFNVGYS
PERINPGDKTHRFETITKVVSAQNPQTLQIVAEVYGSVVKAGIHRAPSIRVAEAAKVIENTQRDLNIAFMNELSLIFQAL
EIDTGDVLAAAATKWNFLPFTPGLVGGHCIGVDPYYLTYRAEKAGYHPEVILAGRRINDEMGRRIARECIRGLLKRKGRS
GVVTILGMTFKENVPDIRNSRVIDIISELQSFGIDVQVADPLADAAAVHEEYGIALTAAEALRPADAVVLAVSHDQFLAG
GWPLVQGLLREGSGLVLDVKMKLDRASKPGAIELWRP

Sequences:

>Translated_437_residues
MPPKRPEYQFMSHGRKIAVIGLGYVGLPVAAAFARAGCPVIGFDIDAARVAELRDGRDRTCEVEAADLKRPGLRFTSDPA
DMAAADFFIVTVPTPIDSARRPDLSAMRSATRTVRAVLKRGDIVVYESTVYPGAVEEDCVPLLESGSELKAGLDFNVGYS
PERINPGDKTHRFETITKVVSAQNPQTLQIVAEVYGSVVKAGIHRAPSIRVAEAAKVIENTQRDLNIAFMNELSLIFQAL
EIDTGDVLAAAATKWNFLPFTPGLVGGHCIGVDPYYLTYRAEKAGYHPEVILAGRRINDEMGRRIARECIRGLLKRKGRS
GVVTILGMTFKENVPDIRNSRVIDIISELQSFGIDVQVADPLADAAAVHEEYGIALTAAEALRPADAVVLAVSHDQFLAG
GWPLVQGLLREGSGLVLDVKMKLDRASKPGAIELWRP
>Mature_436_residues
PPKRPEYQFMSHGRKIAVIGLGYVGLPVAAAFARAGCPVIGFDIDAARVAELRDGRDRTCEVEAADLKRPGLRFTSDPAD
MAAADFFIVTVPTPIDSARRPDLSAMRSATRTVRAVLKRGDIVVYESTVYPGAVEEDCVPLLESGSELKAGLDFNVGYSP
ERINPGDKTHRFETITKVVSAQNPQTLQIVAEVYGSVVKAGIHRAPSIRVAEAAKVIENTQRDLNIAFMNELSLIFQALE
IDTGDVLAAAATKWNFLPFTPGLVGGHCIGVDPYYLTYRAEKAGYHPEVILAGRRINDEMGRRIARECIRGLLKRKGRSG
VVTILGMTFKENVPDIRNSRVIDIISELQSFGIDVQVADPLADAAAVHEEYGIALTAAEALRPADAVVLAVSHDQFLAGG
WPLVQGLLREGSGLVLDVKMKLDRASKPGAIELWRP

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI48994968, Length=409, Percent_Identity=31.7848410757946, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17560350, Length=458, Percent_Identity=26.2008733624454, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17136908, Length=371, Percent_Identity=23.7196765498652, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 47423; Mature: 47292

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPKRPEYQFMSHGRKIAVIGLGYVGLPVAAAFARAGCPVIGFDIDAARVAELRDGRDRT
CCCCCCCHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCE
CEVEAADLKRPGLRFTSDPADMAAADFFIVTVPTPIDSARRPDLSAMRSATRTVRAVLKR
EEEEHHHCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHC
GDIVVYESTVYPGAVEEDCVPLLESGSELKAGLDFNVGYSPERINPGDKTHRFETITKVV
CCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHH
SAQNPQTLQIVAEVYGSVVKAGIHRAPSIRVAEAAKVIENTQRDLNIAFMNELSLIFQAL
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHH
EIDTGDVLAAAATKWNFLPFTPGLVGGHCIGVDPYYLTYRAEKAGYHPEVILAGRRINDE
HCCCCCEEEEHHCCCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCEEEEECCCCCHH
MGRRIARECIRGLLKRKGRSGVVTILGMTFKENVPDIRNSRVIDIISELQSFGIDVQVAD
HHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECC
PLADAAAVHEEYGIALTAAEALRPADAVVLAVSHDQFLAGGWPLVQGLLREGSGLVLDVK
CHHHHHHHHHHCCEEEEHHHHCCCCCEEEEEEECCCEECCCHHHHHHHHHCCCCEEEEEE
MKLDRASKPGAIELWRP
EEECCCCCCCEEEECCC
>Mature Secondary Structure 
PPKRPEYQFMSHGRKIAVIGLGYVGLPVAAAFARAGCPVIGFDIDAARVAELRDGRDRT
CCCCCCHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCE
CEVEAADLKRPGLRFTSDPADMAAADFFIVTVPTPIDSARRPDLSAMRSATRTVRAVLKR
EEEEHHHCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHC
GDIVVYESTVYPGAVEEDCVPLLESGSELKAGLDFNVGYSPERINPGDKTHRFETITKVV
CCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHH
SAQNPQTLQIVAEVYGSVVKAGIHRAPSIRVAEAAKVIENTQRDLNIAFMNELSLIFQAL
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHH
EIDTGDVLAAAATKWNFLPFTPGLVGGHCIGVDPYYLTYRAEKAGYHPEVILAGRRINDE
HCCCCCEEEEHHCCCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCEEEEECCCCCHH
MGRRIARECIRGLLKRKGRSGVVTILGMTFKENVPDIRNSRVIDIISELQSFGIDVQVAD
HHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECC
PLADAAAVHEEYGIALTAAEALRPADAVVLAVSHDQFLAGGWPLVQGLLREGSGLVLDVK
CHHHHHHHHHHCCEEEEHHHHCCCCCEEEEEEECCCEECCCHHHHHHHHHCCCCEEEEEE
MKLDRASKPGAIELWRP
EEECCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]