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The map label for this gene is manC [H]

Identifier: 146342015

GI number: 146342015

Start: 5356554

End: 5357966

Strand: Reverse

Name: manC [H]

Synonym: BRADO5152

Alternate gene names: 146342015

Gene position: 5357966-5356554 (Counterclockwise)

Preceding gene: 146342016

Following gene: 146342014

Centisome position: 71.86

GC content: 67.09

Gene sequence:

>1413_bases
ATGACCGGACGGATCATTCCCCTCATCATGTGCGGCGGCGCTGGAACCCGGCTGTGGCCGGCCTCGCGCGAGGTCAGGCC
GAAACAGTTCCTGCCGCTGTTCGGCGCCCGCTCGACCTTCCAGGACACGCTGCTGCGCGTCTCCGACGCCGATCTGTTTG
CGCGTCCCATCATCATCACCAACGCCGCCTATCGTTTCATGGTGCTGGAGCAACTCGCGGAGATCGGGATCGAGGCTGAC
GTGCTGCTGGAGCCGATGCGGCGCGATTCCGGGCCCGCGATCGCCGCGGGCGCAGCGTTTGCGCAGTCGCGCGATTCCGA
GGCCGTGGTGCTGGCGCTCGCCGCCGACCACGTGGTGCGGGATACCGCAGCGTTCCTCGCCGCGTGCCGCGCCGGGCTCG
CCGCGGCCGAGGCCGGCCGCATCGTCACCTTCGGTGTCAATCCGGAGCGCGCGGCCACCGAATACGGCTATATCAGCCCC
GGCGCGCCGGTCTCCGGAGAGGTACGGGCGGTCGCCAGATTCGTCGAGAAGCCGGACCGGCCGACCGCCGAGAGCTACAT
CCGCGAGGGCTATCTCTGGAACAGCGGCAACTTCATGTTTCGCGCCTCTGTCCTGCTCGACGAATACCGCAAGTACGATA
CCCCGAGCGTGCAGACGGTGAGCGACGCCGTGGCCAAGGCCGGACGCGACCTCAGCTTCATCATCCTGGACTCCGACGCC
TTCGCGGCAGCCAAGCCGATCTCGATCGACTATGCCGTCATGGAGAAGACCGAGCGGGCCGCCGTGGCCCCGGTGTCCTG
CGGCTGGTCCGATGTCGGCTCCTGGCATGCGGTCTGGGAATTGTCGGACAAGGACGGGCAGGGCAATGCCGCCCGCGGCA
CCGCCGTCTTCGAAGATTCCCGCAATTGCAACGTCGTCACCGACAAGGCGCTGGTCGCACTCGAAGGCGTCGACGATCTC
GTCGTGGTGGCCGCGCAGGACGCCGTGCTGGTGTCGCGCCAGCGCGACGCCAACGGGCTGAAGCGGCTCGTGGCCAAGCT
GAAGACGGTCGCGCCGGAGGTGACCGAGGCTCACATCAAGGTGCACCGCCCCTGGGGCAGCTATCAGTCGGTCGACAATG
GCGAGCGCCACCAGGTCAAGCGCATCGTCGTCAAGCCCGGCCAGCGGCTGTCGCTGCAGAAGCACTATCACCGCTCGGAG
CATTGGATCGTGGTGCGCGGCGCGGCGCGGGTCACCGTCAACGAGACCGTCAAGACCGTCCACGAGAACGAATCGATCTA
CATCCCGATGGGCGCGGTGCACCGGCTGGAAAACCCCGGCAAGATCCTGCTGGAACTGATCGAGGTCCAGACCGGATCCT
ATCTCGGCGAGGACGACATCATCCGCATCGAGGACGATTACCGCCGCGAGTAG

Upstream 100 bases:

>100_bases
GCGCGATATCGGCTTCCGGCCAGCGACCTCGATCGTCGACGGCATCGCGCGGTTCGCAAGGTGGTATCGCGACTATCATC
GGATTTGAGGATCTCATTTC

Downstream 100 bases:

>100_bases
GGCGGGCTCTCCCCGCGAATCACGCGAGCCGTGCTAGATAGCGCCGGAGTCCTCCGCCGGAGCGCATCCTTGCAAAGCAC
GATCGGATCGCGCGCGTTCC

Product: mannose-1-phosphate guanyltransferase capsular polysaccharide biosynthesis protein

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTGRIIPLIMCGGAGTRLWPASREVRPKQFLPLFGARSTFQDTLLRVSDADLFARPIIITNAAYRFMVLEQLAEIGIEAD
VLLEPMRRDSGPAIAAGAAFAQSRDSEAVVLALAADHVVRDTAAFLAACRAGLAAAEAGRIVTFGVNPERAATEYGYISP
GAPVSGEVRAVARFVEKPDRPTAESYIREGYLWNSGNFMFRASVLLDEYRKYDTPSVQTVSDAVAKAGRDLSFIILDSDA
FAAAKPISIDYAVMEKTERAAVAPVSCGWSDVGSWHAVWELSDKDGQGNAARGTAVFEDSRNCNVVTDKALVALEGVDDL
VVVAAQDAVLVSRQRDANGLKRLVAKLKTVAPEVTEAHIKVHRPWGSYQSVDNGERHQVKRIVVKPGQRLSLQKHYHRSE
HWIVVRGAARVTVNETVKTVHENESIYIPMGAVHRLENPGKILLELIEVQTGSYLGEDDIIRIEDDYRRE

Sequences:

>Translated_470_residues
MTGRIIPLIMCGGAGTRLWPASREVRPKQFLPLFGARSTFQDTLLRVSDADLFARPIIITNAAYRFMVLEQLAEIGIEAD
VLLEPMRRDSGPAIAAGAAFAQSRDSEAVVLALAADHVVRDTAAFLAACRAGLAAAEAGRIVTFGVNPERAATEYGYISP
GAPVSGEVRAVARFVEKPDRPTAESYIREGYLWNSGNFMFRASVLLDEYRKYDTPSVQTVSDAVAKAGRDLSFIILDSDA
FAAAKPISIDYAVMEKTERAAVAPVSCGWSDVGSWHAVWELSDKDGQGNAARGTAVFEDSRNCNVVTDKALVALEGVDDL
VVVAAQDAVLVSRQRDANGLKRLVAKLKTVAPEVTEAHIKVHRPWGSYQSVDNGERHQVKRIVVKPGQRLSLQKHYHRSE
HWIVVRGAARVTVNETVKTVHENESIYIPMGAVHRLENPGKILLELIEVQTGSYLGEDDIIRIEDDYRRE
>Mature_469_residues
TGRIIPLIMCGGAGTRLWPASREVRPKQFLPLFGARSTFQDTLLRVSDADLFARPIIITNAAYRFMVLEQLAEIGIEADV
LLEPMRRDSGPAIAAGAAFAQSRDSEAVVLALAADHVVRDTAAFLAACRAGLAAAEAGRIVTFGVNPERAATEYGYISPG
APVSGEVRAVARFVEKPDRPTAESYIREGYLWNSGNFMFRASVLLDEYRKYDTPSVQTVSDAVAKAGRDLSFIILDSDAF
AAAKPISIDYAVMEKTERAAVAPVSCGWSDVGSWHAVWELSDKDGQGNAARGTAVFEDSRNCNVVTDKALVALEGVDDLV
VVAAQDAVLVSRQRDANGLKRLVAKLKTVAPEVTEAHIKVHRPWGSYQSVDNGERHQVKRIVVKPGQRLSLQKHYHRSEH
WIVVRGAARVTVNETVKTVHENESIYIPMGAVHRLENPGKILLELIEVQTGSYLGEDDIIRIEDDYRRE

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=474, Percent_Identity=45.9915611814346, Blast_Score=413, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR018247
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 51491; Mature: 51360

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRIIPLIMCGGAGTRLWPASREVRPKQFLPLFGARSTFQDTLLRVSDADLFARPIIIT
CCCCEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHEECCCCHHCCCEEEE
NAAYRFMVLEQLAEIGIEADVLLEPMRRDSGPAIAAGAAFAQSRDSEAVVLALAADHVVR
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEHHHHHHCCCCCCEEEEEEEHHHHHH
DTAAFLAACRAGLAAAEAGRIVTFGVNPERAATEYGYISPGAPVSGEVRAVARFVEKPDR
HHHHHHHHHHHCHHHHCCCCEEEECCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCC
PTAESYIREGYLWNSGNFMFRASVLLDEYRKYDTPSVQTVSDAVAKAGRDLSFIILDSDA
CCHHHHHHCCEEECCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCC
FAAAKPISIDYAVMEKTERAAVAPVSCGWSDVGSWHAVWELSDKDGQGNAARGTAVFEDS
CCCCCCCEEEHHHHHCCCCCEECEECCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECC
RNCNVVTDKALVALEGVDDLVVVAAQDAVLVSRQRDANGLKRLVAKLKTVAPEVTEAHIK
CCCCEEECCEEEEECCCCCEEEEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEE
VHRPWGSYQSVDNGERHQVKRIVVKPGQRLSLQKHYHRSEHWIVVRGAARVTVNETVKTV
EECCCCCCCCCCCCCHHHHHEEEECCCCCCCHHHHHCCCCCEEEEECCEEEEHHHHHHHH
HENESIYIPMGAVHRLENPGKILLELIEVQTGSYLGEDDIIRIEDDYRRE
HCCCEEEEECHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECHHCCCC
>Mature Secondary Structure 
TGRIIPLIMCGGAGTRLWPASREVRPKQFLPLFGARSTFQDTLLRVSDADLFARPIIIT
CCCEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHEECCCCHHCCCEEEE
NAAYRFMVLEQLAEIGIEADVLLEPMRRDSGPAIAAGAAFAQSRDSEAVVLALAADHVVR
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEHHHHHHCCCCCCEEEEEEEHHHHHH
DTAAFLAACRAGLAAAEAGRIVTFGVNPERAATEYGYISPGAPVSGEVRAVARFVEKPDR
HHHHHHHHHHHCHHHHCCCCEEEECCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCC
PTAESYIREGYLWNSGNFMFRASVLLDEYRKYDTPSVQTVSDAVAKAGRDLSFIILDSDA
CCHHHHHHCCEEECCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCC
FAAAKPISIDYAVMEKTERAAVAPVSCGWSDVGSWHAVWELSDKDGQGNAARGTAVFEDS
CCCCCCCEEEHHHHHCCCCCEECEECCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECC
RNCNVVTDKALVALEGVDDLVVVAAQDAVLVSRQRDANGLKRLVAKLKTVAPEVTEAHIK
CCCCEEECCEEEEECCCCCEEEEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEE
VHRPWGSYQSVDNGERHQVKRIVVKPGQRLSLQKHYHRSEHWIVVRGAARVTVNETVKTV
EECCCCCCCCCCCCCHHHHHEEEECCCCCCCHHHHHCCCCCEEEEECCEEEEHHHHHHHH
HENESIYIPMGAVHRLENPGKILLELIEVQTGSYLGEDDIIRIEDDYRRE
HCCCEEEEECHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1383393 [H]