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The map label for this gene is rffG [C]

Identifier: 146342016

GI number: 146342016

Start: 5357979

End: 5358995

Strand: Reverse

Name: rffG [C]

Synonym: BRADO5153

Alternate gene names: 146342016

Gene position: 5358995-5357979 (Counterclockwise)

Preceding gene: 146342017

Following gene: 146342015

Centisome position: 71.87

GC content: 62.83

Gene sequence:

>1017_bases
ATGGCAAACGAACCCATCCTCGTCACCGGCGCCGCCGGCTTCATCGGGTTTCATCTGACCCAGCAACTGCTCGCTGAGGG
GCGGCAGGTCGTCGGCCTCGACAACATCAATTCCTACTACGATCCGACGCTCAAGGAGGCGCGACTTGCCCTCCTGCAGG
CGCAGCCCGGCTTCAGCTTCCACAAGCTCGATCTCGTCGACCGCGCCGCCATCAAGGCGCTGTTCGCCGCGCACCGCTTT
CCCGCCGTGGTGCATCTCGCCGCGCAGGCCGGGGTGCGCTACTCGCTGGAAAATCCGCACGCCTATGTCGATGCCAACCT
CGAGGGCTTCATCAACGTGCTCGAGGGCTGCCGGCACCACGGCTGCGGGCATCTGCTGTTCGCCTCCTCGTCGTCTGTCT
ATGGCGCCAACACCAAGCTGCCGTTCTCGGTCAAGGACAATGTCGACCATCCGATCAGCCTCTATGCGGCGAGCAAGAAG
GCGAACGAGCTGATGGCCCACTCCTACAGCCATCTGTACCGGTTGCCGGCCACCGGCCTGCGCTTCTTTACCGTCTATGG
TCCATGGGGCCGGCCCGACATGGCGATGTTCATCTTTGCCAAGGCCATCCTCGCCGGACAGCCGGTCAGGCTGTTCAATC
ACGGCCAGATGCGGCGCGATTTTACCTATGTGGACGACATCGTGCAGGCCATCGTGCGATTGATCGGCCGTCCGCCGCAG
GGAAACCCGGATTGGAACGGAAACACGCCGGATCCGTCGAGCAGCCGGGCACCATGGCGGATTTACAATATCGGCAACAA
TCATCCCGAGCAGCTGACCGACGTGATAACCCTCCTGGAGAAGGAGTTCGGGCGGCCGGCGATCAAGGAGATGCTGCCGA
TGCAGCCCGGAGACGTCGAGGCGACCTACGCCGATGTCAGCGATCTCGAGCGCGATATCGGCTTCCGGCCAGCGACCTCG
ATCGTCGACGGCATCGCGCGGTTCGCAAGGTGGTATCGCGACTATCATCGGATTTGA

Upstream 100 bases:

>100_bases
CTGCGCGAGGGGAGCGGCCTCGTACTCGATGTCAAGATGAAGCTCGACCGCGCGAGCAAACCCGGCGCCATCGAGCTGTG
GCGGCCCTGACGGAGCGACC

Downstream 100 bases:

>100_bases
GGATCTCATTTCATGACCGGACGGATCATTCCCCTCATCATGTGCGGCGGCGCTGGAACCCGGCTGTGGCCGGCCTCGCG
CGAGGTCAGGCCGAAACAGT

Product: nucleotide sugar epimerase capsular polysaccharide biosynthesis protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MANEPILVTGAAGFIGFHLTQQLLAEGRQVVGLDNINSYYDPTLKEARLALLQAQPGFSFHKLDLVDRAAIKALFAAHRF
PAVVHLAAQAGVRYSLENPHAYVDANLEGFINVLEGCRHHGCGHLLFASSSSVYGANTKLPFSVKDNVDHPISLYAASKK
ANELMAHSYSHLYRLPATGLRFFTVYGPWGRPDMAMFIFAKAILAGQPVRLFNHGQMRRDFTYVDDIVQAIVRLIGRPPQ
GNPDWNGNTPDPSSSRAPWRIYNIGNNHPEQLTDVITLLEKEFGRPAIKEMLPMQPGDVEATYADVSDLERDIGFRPATS
IVDGIARFARWYRDYHRI

Sequences:

>Translated_338_residues
MANEPILVTGAAGFIGFHLTQQLLAEGRQVVGLDNINSYYDPTLKEARLALLQAQPGFSFHKLDLVDRAAIKALFAAHRF
PAVVHLAAQAGVRYSLENPHAYVDANLEGFINVLEGCRHHGCGHLLFASSSSVYGANTKLPFSVKDNVDHPISLYAASKK
ANELMAHSYSHLYRLPATGLRFFTVYGPWGRPDMAMFIFAKAILAGQPVRLFNHGQMRRDFTYVDDIVQAIVRLIGRPPQ
GNPDWNGNTPDPSSSRAPWRIYNIGNNHPEQLTDVITLLEKEFGRPAIKEMLPMQPGDVEATYADVSDLERDIGFRPATS
IVDGIARFARWYRDYHRI
>Mature_337_residues
ANEPILVTGAAGFIGFHLTQQLLAEGRQVVGLDNINSYYDPTLKEARLALLQAQPGFSFHKLDLVDRAAIKALFAAHRFP
AVVHLAAQAGVRYSLENPHAYVDANLEGFINVLEGCRHHGCGHLLFASSSSVYGANTKLPFSVKDNVDHPISLYAASKKA
NELMAHSYSHLYRLPATGLRFFTVYGPWGRPDMAMFIFAKAILAGQPVRLFNHGQMRRDFTYVDDIVQAIVRLIGRPPQG
NPDWNGNTPDPSSSRAPWRIYNIGNNHPEQLTDVITLLEKEFGRPAIKEMLPMQPGDVEATYADVSDLERDIGFRPATSI
VDGIARFARWYRDYHRI

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI56237023, Length=352, Percent_Identity=26.9886363636364, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI56118217, Length=352, Percent_Identity=26.9886363636364, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI189083684, Length=352, Percent_Identity=26.9886363636364, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=25.2225519287834, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=23.5820895522388, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI48994969, Length=349, Percent_Identity=25.5014326647564, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788353, Length=354, Percent_Identity=24.2937853107345, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1786974, Length=355, Percent_Identity=25.0704225352113, Blast_Score=98, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=349, Percent_Identity=26.0744985673352, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71982038, Length=351, Percent_Identity=25.6410256410256, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17568069, Length=346, Percent_Identity=25.1445086705202, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=336, Percent_Identity=24.1071428571429, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=137, Percent_Identity=34.3065693430657, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19923002, Length=353, Percent_Identity=24.0793201133144, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21356223, Length=335, Percent_Identity=22.3880597014925, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37698; Mature: 37567

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANEPILVTGAAGFIGFHLTQQLLAEGRQVVGLDNINSYYDPTLKEARLALLQAQPGFSF
CCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCC
HKLDLVDRAAIKALFAAHRFPAVVHLAAQAGVRYSLENPHAYVDANLEGFINVLEGCRHH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCHHHHHHHHHHHHHC
GCGHLLFASSSSVYGANTKLPFSVKDNVDHPISLYAASKKANELMAHSYSHLYRLPATGL
CCCEEEEECCCCCCCCCCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCE
RFFTVYGPWGRPDMAMFIFAKAILAGQPVRLFNHGQMRRDFTYVDDIVQAIVRLIGRPPQ
EEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC
GNPDWNGNTPDPSSSRAPWRIYNIGNNHPEQLTDVITLLEKEFGRPAIKEMLPMQPGDVE
CCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCC
ATYADVSDLERDIGFRPATSIVDGIARFARWYRDYHRI
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANEPILVTGAAGFIGFHLTQQLLAEGRQVVGLDNINSYYDPTLKEARLALLQAQPGFSF
CCCCEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCC
HKLDLVDRAAIKALFAAHRFPAVVHLAAQAGVRYSLENPHAYVDANLEGFINVLEGCRHH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCHHHHHHHHHHHHHC
GCGHLLFASSSSVYGANTKLPFSVKDNVDHPISLYAASKKANELMAHSYSHLYRLPATGL
CCCEEEEECCCCCCCCCCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCE
RFFTVYGPWGRPDMAMFIFAKAILAGQPVRLFNHGQMRRDFTYVDDIVQAIVRLIGRPPQ
EEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC
GNPDWNGNTPDPSSSRAPWRIYNIGNNHPEQLTDVITLLEKEFGRPAIKEMLPMQPGDVE
CCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCC
ATYADVSDLERDIGFRPATSIVDGIARFARWYRDYHRI
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]