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The map label for this gene is dksA [H]

Identifier: 146341910

GI number: 146341910

Start: 5236035

End: 5236436

Strand: Reverse

Name: dksA [H]

Synonym: BRADO5045

Alternate gene names: 146341910

Gene position: 5236436-5236035 (Counterclockwise)

Preceding gene: 146341911

Following gene: 146341909

Centisome position: 70.23

GC content: 62.69

Gene sequence:

>402_bases
TTGAAGAACTACAAACCTTCCGAAAAAGAGCCTTTCATGAACGACCGCCAGCGCGACTACTTCCGCGCCAAGCTGCTGGC
ATGGAAGGAGGAGATCCTGCGCGAATCGAAGCTCACCCTGCAGGCTCTGCAGGAGGAGAACGTGAACCACCCCGACCTCG
CCGATCGCGCGTCGTCGGAGACCGACCGGGCGATCGAGCTTCGCGCACGCGACCGCCAGCGCAAGCTGATCTCCAAGATC
GACGCCGCGCTCCAGCGCATCGAGGACAACACCTACGGCTATTGCGAGGAGACCGGCGAGCCGATTTCGCTGAAGCGGCT
CGAGGCCCGTCCGATCGCGACGCTGTCGGTGGAGGCGCAGGAGCGCCACGAGAAGCGTGAAAAGGTCTATCGCGACGAAT
AG

Upstream 100 bases:

>100_bases
CACATGAGGCCGCTATATAACGCGGCCGCGAACGGGCGTATTCCGTCCGCCGGTGCCGCGACCGGCGCCAGCACGTACAG
ATGGGCTGACCTTGGAAAAG

Downstream 100 bases:

>100_bases
GTCGCGGCGCTGACCAGACGAGCCCGATTGCGGCCGCCCGCCGGGTGCGGCCGCGTCACCGTTTGGTCTGCGATCACGCT
GAACAGGTCGGTCCGTTGAA

Product: DnaK suppressor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MKNYKPSEKEPFMNDRQRDYFRAKLLAWKEEILRESKLTLQALQEENVNHPDLADRASSETDRAIELRARDRQRKLISKI
DAALQRIEDNTYGYCEETGEPISLKRLEARPIATLSVEAQERHEKREKVYRDE

Sequences:

>Translated_133_residues
MKNYKPSEKEPFMNDRQRDYFRAKLLAWKEEILRESKLTLQALQEENVNHPDLADRASSETDRAIELRARDRQRKLISKI
DAALQRIEDNTYGYCEETGEPISLKRLEARPIATLSVEAQERHEKREKVYRDE
>Mature_133_residues
MKNYKPSEKEPFMNDRQRDYFRAKLLAWKEEILRESKLTLQALQEENVNHPDLADRASSETDRAIELRARDRQRKLISKI
DAALQRIEDNTYGYCEETGEPISLKRLEARPIATLSVEAQERHEKREKVYRDE

Specific function: Dosage-Dependent Suppressor Of A Dnak Deletion Mutation. It Suppressed Not Only The Temperature-Sensitive Growth But Also The Filamentous Phenotype Of The Dnak Deletion Strain, While The Defect Of Lambda Growth Is Not Suppressed. [C]

COG id: COG1734

COG function: function code T; DnaK suppressor protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 dksA C4-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786338, Length=125, Percent_Identity=43.2, Blast_Score=106, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012784
- InterPro:   IPR000962
- InterPro:   IPR020458 [H]

Pfam domain/function: PF01258 zf-dskA_traR [H]

EC number: NA

Molecular weight: Translated: 15820; Mature: 15820

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS01102 ZF_DKSA_1 ; PS51128 ZF_DKSA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYKPSEKEPFMNDRQRDYFRAKLLAWKEEILRESKLTLQALQEENVNHPDLADRASSE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCC
TDRAIELRARDRQRKLISKIDAALQRIEDNTYGYCEETGEPISLKRLEARPIATLSVEAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCEEEECCHH
ERHEKREKVYRDE
HHHHHHHHHCCCC
>Mature Secondary Structure
MKNYKPSEKEPFMNDRQRDYFRAKLLAWKEEILRESKLTLQALQEENVNHPDLADRASSE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCC
TDRAIELRARDRQRKLISKIDAALQRIEDNTYGYCEETGEPISLKRLEARPIATLSVEAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCEEEECCHH
ERHEKREKVYRDE
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9555902 [H]