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The map label for this gene is bluB [H]

Identifier: 146341777

GI number: 146341777

Start: 5096269

End: 5096787

Strand: Reverse

Name: bluB [H]

Synonym: BRADO4896

Alternate gene names: 146341777

Gene position: 5096787-5096269 (Counterclockwise)

Preceding gene: 146341778

Following gene: 146341774

Centisome position: 68.35

GC content: 68.59

Gene sequence:

>519_bases
ATGCTCGCCTCCTACGCGCCTTCGGTCGGTTTTTGCCAGCCATGGCGCTACGTGCTGGTCGAGAGCGCGGCGCGGCGTGC
GGCTGTCATCGAGAATTTCAAGTCGGCCAATGAGGCGGCGCTGGCGGGCTATGACGGCGAGCGGCGGGCGCTGTATGCGC
GACTGAAGCTGGAAGGACTGACGCAGGCGCCGGTGCATCTCGTGGTCTGCGCCGACGAGGGCGCGGCGACCGGCCACCGT
CTCGGCCGCGCCACGATGCCTGAAACCCTGCGCTACTCAGTGGTGGCGGCTGTCCAGACGTTCTGGCTGGCGGCGCGCGC
GGAGGGGCTCGGCGTCGGCTGGGTATCGATCCTCGATCCCTTGGCCGCGTGCAGGAAGCTCGAGCTCCCGCCCGACTGGT
CCTTGATCGCCTATCTCTGCGTCGGCTGGCCGGAGGAGGAGCATGACGATCCCGAACTGGAGCGTCACGGCTGGGAGAGG
CGGCTCGATCCGTCGGCGATCGAGCCGCTGAAGCGCTAA

Upstream 100 bases:

>100_bases
CGCCTTCAAGCAACGTTTCGCCGAGCTCGTGCGCTGGCGCCGCGACGTCCGGCGGTTTCGCACCGATCCTGTTGACCCGG
AACTGATCGACCATCTGCTC

Downstream 100 bases:

>100_bases
TTAGTTAGCGCATGATCCGGGAGAGCCGCTCTTCCAACCGGATCACTCCTCTCACGACGCCAGTGCCAGCGCAGGTGCGT
CCGGCGCGACGGGAGCTGCA

Product: putative Cob(II)yrinic acid a,c-diamide reductase, bluB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MLASYAPSVGFCQPWRYVLVESAARRAAVIENFKSANEAALAGYDGERRALYARLKLEGLTQAPVHLVVCADEGAATGHR
LGRATMPETLRYSVVAAVQTFWLAARAEGLGVGWVSILDPLAACRKLELPPDWSLIAYLCVGWPEEEHDDPELERHGWER
RLDPSAIEPLKR

Sequences:

>Translated_172_residues
MLASYAPSVGFCQPWRYVLVESAARRAAVIENFKSANEAALAGYDGERRALYARLKLEGLTQAPVHLVVCADEGAATGHR
LGRATMPETLRYSVVAAVQTFWLAARAEGLGVGWVSILDPLAACRKLELPPDWSLIAYLCVGWPEEEHDDPELERHGWER
RLDPSAIEPLKR
>Mature_172_residues
MLASYAPSVGFCQPWRYVLVESAARRAAVIENFKSANEAALAGYDGERRALYARLKLEGLTQAPVHLVVCADEGAATGHR
LGRATMPETLRYSVVAAVQTFWLAARAEGLGVGWVSILDPLAACRKLELPPDWSLIAYLCVGWPEEEHDDPELERHGWER
RLDPSAIEPLKR

Specific function: Maybe catalyzes the reduction of Co(2+) to Co(+) in cob(II)yrinic acid A,C-diamide to allow adenosylation to procede [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012825
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.16.8.1 [H]

Molecular weight: Translated: 19040; Mature: 19040

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLASYAPSVGFCQPWRYVLVESAARRAAVIENFKSANEAALAGYDGERRALYARLKLEGL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHEEEEEEEECCC
TQAPVHLVVCADEGAATGHRLGRATMPETLRYSVVAAVQTFWLAARAEGLGVGWVSILDP
CCCCEEEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LAACRKLELPPDWSLIAYLCVGWPEEEHDDPELERHGWERRLDPSAIEPLKR
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCHHCCCCCHHHCCCCC
>Mature Secondary Structure
MLASYAPSVGFCQPWRYVLVESAARRAAVIENFKSANEAALAGYDGERRALYARLKLEGL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHEEEEEEEECCC
TQAPVHLVVCADEGAATGHRLGRATMPETLRYSVVAAVQTFWLAARAEGLGVGWVSILDP
CCCCEEEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LAACRKLELPPDWSLIAYLCVGWPEEEHDDPELERHGWERRLDPSAIEPLKR
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCHHCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7635831; 9256491 [H]