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The map label for this gene is cobB [H]

Identifier: 146341778

GI number: 146341778

Start: 5096922

End: 5098262

Strand: Reverse

Name: cobB [H]

Synonym: BRADO4897

Alternate gene names: 146341778

Gene position: 5098262-5096922 (Counterclockwise)

Preceding gene: 146341780

Following gene: 146341777

Centisome position: 68.37

GC content: 68.83

Gene sequence:

>1341_bases
ATGACCGGAACGGCGACCAAGGGACTTATCATCGCGGCCCCGCGCTCGGGGGCGGGGAAGACGACCATCACGCTCGGGCT
GTTGCGGGCGCTGGTGCGTCGCGGCGTGCGCGTGCAGCCGTTCAAATGCGGTCCCGACTACATCGATCTCGCCTTTCATG
CGGTCGCGGCCGAACGGACGAGCTACACGCTCGACAGCTGGGCGATGTCGAGGCCGCAGATCACCGGGCTGGTCTCCGAC
GTCGCTGCCGGCGCCGATTTCTCGATCGTCGAAGGTGTCATGGGCCTGTTCGACGGCGCGCCGCAGGCCGGTCGCGCCGC
CCGTGGCTCGGCCGCGGATCTCGCCGCGCTGCTCGGCTGGCCGGTGCTGCTCGTCCTCGATGTCAGCGGCCAGACCGAGA
CGGCGGCAGCGATCGCGCTCGGCTGCGCGCGCTTCCGCGACGACGTCGTCATCGCCGGCGTGATTCTCAATCAGGTTGCG
AGCGAGCGGCACAGGGCGCTGATCGCGCCCGCCTTCGCCACCATCGGCATCGACGTGCTGGGCGCGCTGGCGCGTGACCG
GCAACTGACCTTGCCGGAGCGTCATCTCGGCCTGGTCCAGGCCCAGGAGATCACCGCGATCGAGACGCATCTCGATCGGC
TGGCGGCGCAGATCTCCCAGTCGGTCGATCTGGGCGCAGTGCAGGCGCTTGCGCGGCCGATGCGCACATCCGGCGAAGCC
GTGGAGCGATCCGATTTCGGCATCAATCCTCCAGGGCAGCGCATCGCACTCGCATCGGACGCCGCATTCTCTTTCAACTA
TCCGCATCTGCAGCGGCACTGGCGGGCCGCAGGAGCCGACATCGTGCCGTTCTCGCCGCTGAATGACGACGCGCCCGATC
CGTCGGCTGATGCCGTGTGGCTGCCCGGCGGCTATCCCGAATTGCATGCCGGCCGCCTGGCGTCGGCGCCATCCTTCCTG
CAAGGCCTTCGCGCGATGGCCGCGCGCGGCGTTCCGATCCACGGCGAGTGTGGCGGCTACATGATGTTGGGAGCGGGGTT
GGAAGATTCTGATGGTACGCGGCACGCGATGGCGGGGCTGTTGTCGCTGGAAACCTCCTTCGCCAAGCGTCGCCTGCATC
TCGGCTACCGCCGGGCGGCGCTGCTGGCCGACTGCAGCCTCGGCCGAAAAGGGGCGGCCGTTCATGGTCACGAGTTCCAC
TACGCGACGATCCTGCAAGAGGGCGATGACCCCCTGATCGACTGCCGTGATGCGGCGGGACGCGCAACCGCCGAGCGTGG
CGCGCGGCGCGGAAGCGTGACGGGATCGTTTCTGCACGCGCTGAGCGGAGAGAGCGCATGA

Upstream 100 bases:

>100_bases
GTGGTCCAGCCACGCTTCAACGGTCGGTTCGGCGCCACAGCAGATCCCGTCATGGCCGATCGCATAGCATATTTTGCCGG
GCGGCGGCGGGGCCCCGACG

Downstream 100 bases:

>100_bases
GCATGACGAGGCCAGCGCCGCCACGGTTCGATGCCGCCTTCAAGCAACGTTTCGCCGAGCTCGTGCGCTGGCGCCGCGAC
GTCCGGCGGTTTCGCACCGA

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTGTATKGLIIAAPRSGAGKTTITLGLLRALVRRGVRVQPFKCGPDYIDLAFHAVAAERTSYTLDSWAMSRPQITGLVSD
VAAGADFSIVEGVMGLFDGAPQAGRAARGSAADLAALLGWPVLLVLDVSGQTETAAAIALGCARFRDDVVIAGVILNQVA
SERHRALIAPAFATIGIDVLGALARDRQLTLPERHLGLVQAQEITAIETHLDRLAAQISQSVDLGAVQALARPMRTSGEA
VERSDFGINPPGQRIALASDAAFSFNYPHLQRHWRAAGADIVPFSPLNDDAPDPSADAVWLPGGYPELHAGRLASAPSFL
QGLRAMAARGVPIHGECGGYMMLGAGLEDSDGTRHAMAGLLSLETSFAKRRLHLGYRRAALLADCSLGRKGAAVHGHEFH
YATILQEGDDPLIDCRDAAGRATAERGARRGSVTGSFLHALSGESA

Sequences:

>Translated_446_residues
MTGTATKGLIIAAPRSGAGKTTITLGLLRALVRRGVRVQPFKCGPDYIDLAFHAVAAERTSYTLDSWAMSRPQITGLVSD
VAAGADFSIVEGVMGLFDGAPQAGRAARGSAADLAALLGWPVLLVLDVSGQTETAAAIALGCARFRDDVVIAGVILNQVA
SERHRALIAPAFATIGIDVLGALARDRQLTLPERHLGLVQAQEITAIETHLDRLAAQISQSVDLGAVQALARPMRTSGEA
VERSDFGINPPGQRIALASDAAFSFNYPHLQRHWRAAGADIVPFSPLNDDAPDPSADAVWLPGGYPELHAGRLASAPSFL
QGLRAMAARGVPIHGECGGYMMLGAGLEDSDGTRHAMAGLLSLETSFAKRRLHLGYRRAALLADCSLGRKGAAVHGHEFH
YATILQEGDDPLIDCRDAAGRATAERGARRGSVTGSFLHALSGESA
>Mature_445_residues
TGTATKGLIIAAPRSGAGKTTITLGLLRALVRRGVRVQPFKCGPDYIDLAFHAVAAERTSYTLDSWAMSRPQITGLVSDV
AAGADFSIVEGVMGLFDGAPQAGRAARGSAADLAALLGWPVLLVLDVSGQTETAAAIALGCARFRDDVVIAGVILNQVAS
ERHRALIAPAFATIGIDVLGALARDRQLTLPERHLGLVQAQEITAIETHLDRLAAQISQSVDLGAVQALARPMRTSGEAV
ERSDFGINPPGQRIALASDAAFSFNYPHLQRHWRAAGADIVPFSPLNDDAPDPSADAVWLPGGYPELHAGRLASAPSFLQ
GLRAMAARGVPIHGECGGYMMLGAGLEDSDGTRHAMAGLLSLETSFAKRRLHLGYRRAALLADCSLGRKGAAVHGHEFHY
ATILQEGDDPLIDCRDAAGRATAERGARRGSVTGSFLHALSGESA

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 46763; Mature: 46632

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTATKGLIIAAPRSGAGKTTITLGLLRALVRRGVRVQPFKCGPDYIDLAFHAVAAERT
CCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHC
SYTLDSWAMSRPQITGLVSDVAAGADFSIVEGVMGLFDGAPQAGRAARGSAADLAALLGW
CCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCC
PVLLVLDVSGQTETAAAIALGCARFRDDVVIAGVILNQVASERHRALIAPAFATIGIDVL
CEEEEEECCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHEEHHHHHHHHHHHHH
GALARDRQLTLPERHLGLVQAQEITAIETHLDRLAAQISQSVDLGAVQALARPMRTSGEA
HHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCH
VERSDFGINPPGQRIALASDAAFSFNYPHLQRHWRAAGADIVPFSPLNDDAPDPSADAVW
HHHCCCCCCCCCCEEEEECCCEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
LPGGYPELHAGRLASAPSFLQGLRAMAARGVPIHGECGGYMMLGAGLEDSDGTRHAMAGL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCCHHHHHHHH
LSLETSFAKRRLHLGYRRAALLADCSLGRKGAAVHGHEFHYATILQEGDDPLIDCRDAAG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEEEECCCCCCEECCCCCC
RATAERGARRGSVTGSFLHALSGESA
CHHHHHCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TGTATKGLIIAAPRSGAGKTTITLGLLRALVRRGVRVQPFKCGPDYIDLAFHAVAAERT
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHC
SYTLDSWAMSRPQITGLVSDVAAGADFSIVEGVMGLFDGAPQAGRAARGSAADLAALLGW
CCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCC
PVLLVLDVSGQTETAAAIALGCARFRDDVVIAGVILNQVASERHRALIAPAFATIGIDVL
CEEEEEECCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHEEHHHHHHHHHHHHH
GALARDRQLTLPERHLGLVQAQEITAIETHLDRLAAQISQSVDLGAVQALARPMRTSGEA
HHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCH
VERSDFGINPPGQRIALASDAAFSFNYPHLQRHWRAAGADIVPFSPLNDDAPDPSADAVW
HHHCCCCCCCCCCEEEEECCCEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
LPGGYPELHAGRLASAPSFLQGLRAMAARGVPIHGECGGYMMLGAGLEDSDGTRHAMAGL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCCHHHHHHHH
LSLETSFAKRRLHLGYRRAALLADCSLGRKGAAVHGHEFHYATILQEGDDPLIDCRDAAG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEEEECCCCCCEECCCCCC
RATAERGARRGSVTGSFLHALSGESA
CHHHHHCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]