Click here to switch to the map view.

The map label for this gene is fucA [C]

Identifier: 146341212

GI number: 146341212

Start: 4487609

End: 4488391

Strand: Reverse

Name: fucA [C]

Synonym: BRADO4286

Alternate gene names: 146341212

Gene position: 4488391-4487609 (Counterclockwise)

Preceding gene: 146341213

Following gene: 146341211

Centisome position: 60.19

GC content: 64.24

Gene sequence:

>783_bases
ATGAACGAGATCGTCGAACGACCCAGGAAGTTTGCGCTGGTCGAGCGCGCGCCCGCAGCCACCTTCGAGGAGGAGCGGCT
GCACCGCAAGCAGCGCCTTGCGGCGACCTTCCGCCTGTTCGCGCGCTACGGCTTCGATCAGGGTCTGGCCGGCCACGTCA
CCGTGCGCGATCCGGAATTCCCGGACCGGTTCTGGATCAATCCGCTATCGACGCATTTCAGCCAGATCAAGGTCTCCGAC
CTGCAACTCGTCGATCACGACGGCAACATCCTGATCGGCAACAAGCCGATCAACCAGGCCGGCTTCGTCATTCATTCCGC
GATCCACGCCGCCCATCCGCAGGTGATCGCTGCCGCGCATACGCATTCGACCTACGGCAAGGCGTTCTCCGCGCTGGGTC
GACTGCTCGATCCGCTGACGCAGGATTCCTGCGCCTTCTACGGCGACCACGTGCTCTTCGATCCGTTCACCGGCGTGGTG
CTCGATGCCGAAGAGGGCAAGAAGATCGCGGCCGCGCTCGGAGCCAAGAAGGCTGCGATCCTGCAGAACCACGGTCTGCT
GACGGTGGGACCCACCATCGAGGCGACGGCCTGGTGGTACATCGCGATGGACAACGCCGCGCGGACGCAATTGCTGGCCG
AGGCGGCCGGTCCCACCAAGCCCATCCCGCATGACATCGCGAAGCACACTTCGGGGCAGGTCGGCACCCACAAGGGCGGC
TATTTCAGCTTCCAGCCGCTGTGGGACTGGATCACGGCGCAAGAGCCGGATCTGTTCAACTGA

Upstream 100 bases:

>100_bases
AGGCGATCATCTCGCGCGCCGGCTCAATCGCCGCTTGAGGCAGCGCTGAGCTGACGCAGACACGGATACAATCGTCGCAG
CCGAGCAGGAGGAAACGACC

Downstream 100 bases:

>100_bases
GTCGAACCCCCGTAGCCCGCATGAGCGCCAGCGACATGCGGGTTCTCCTCGGCAGCTCGTTTGATCCCGGATGTCGCTTC
GCTCATCCGGGCTACCGGGA

Product: hypothetical protein

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNEIVERPRKFALVERAPAATFEEERLHRKQRLAATFRLFARYGFDQGLAGHVTVRDPEFPDRFWINPLSTHFSQIKVSD
LQLVDHDGNILIGNKPINQAGFVIHSAIHAAHPQVIAAAHTHSTYGKAFSALGRLLDPLTQDSCAFYGDHVLFDPFTGVV
LDAEEGKKIAAALGAKKAAILQNHGLLTVGPTIEATAWWYIAMDNAARTQLLAEAAGPTKPIPHDIAKHTSGQVGTHKGG
YFSFQPLWDWITAQEPDLFN

Sequences:

>Translated_260_residues
MNEIVERPRKFALVERAPAATFEEERLHRKQRLAATFRLFARYGFDQGLAGHVTVRDPEFPDRFWINPLSTHFSQIKVSD
LQLVDHDGNILIGNKPINQAGFVIHSAIHAAHPQVIAAAHTHSTYGKAFSALGRLLDPLTQDSCAFYGDHVLFDPFTGVV
LDAEEGKKIAAALGAKKAAILQNHGLLTVGPTIEATAWWYIAMDNAARTQLLAEAAGPTKPIPHDIAKHTSGQVGTHKGG
YFSFQPLWDWITAQEPDLFN
>Mature_260_residues
MNEIVERPRKFALVERAPAATFEEERLHRKQRLAATFRLFARYGFDQGLAGHVTVRDPEFPDRFWINPLSTHFSQIKVSD
LQLVDHDGNILIGNKPINQAGFVIHSAIHAAHPQVIAAAHTHSTYGKAFSALGRLLDPLTQDSCAFYGDHVLFDPFTGVV
LDAEEGKKIAAALGAKKAAILQNHGLLTVGPTIEATAWWYIAMDNAARTQLLAEAAGPTKPIPHDIAKHTSGQVGTHKGG
YFSFQPLWDWITAQEPDLFN

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Homo sapiens, GI29826325, Length=203, Percent_Identity=35.9605911330049, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI29826323, Length=203, Percent_Identity=35.9605911330049, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI29826321, Length=203, Percent_Identity=35.9605911330049, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI29826319, Length=203, Percent_Identity=35.9605911330049, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI9257190, Length=229, Percent_Identity=28.82096069869, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI89276787, Length=229, Percent_Identity=28.82096069869, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI297307160, Length=229, Percent_Identity=28.82096069869, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI297307158, Length=229, Percent_Identity=28.82096069869, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI9257192, Length=229, Percent_Identity=28.82096069869, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI9951927, Length=199, Percent_Identity=28.643216080402, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI62912453, Length=199, Percent_Identity=28.643216080402, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI9943848, Length=199, Percent_Identity=28.643216080402, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1789164, Length=199, Percent_Identity=26.6331658291457, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115533216, Length=229, Percent_Identity=31.8777292576419, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI115533218, Length=229, Percent_Identity=31.8777292576419, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71989560, Length=196, Percent_Identity=29.5918367346939, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71989541, Length=196, Percent_Identity=29.5918367346939, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71989555, Length=196, Percent_Identity=29.5918367346939, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71989550, Length=196, Percent_Identity=30.1020408163265, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71989544, Length=196, Percent_Identity=29.5918367346939, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281363755, Length=186, Percent_Identity=34.9462365591398, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281363751, Length=186, Percent_Identity=34.9462365591398, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24655690, Length=186, Percent_Identity=34.9462365591398, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24655685, Length=186, Percent_Identity=34.9462365591398, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI281363763, Length=184, Percent_Identity=35.3260869565217, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI281363757, Length=184, Percent_Identity=35.3260869565217, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17136738, Length=184, Percent_Identity=35.3260869565217, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI281363761, Length=184, Percent_Identity=35.3260869565217, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI281363759, Length=184, Percent_Identity=35.3260869565217, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI281363753, Length=184, Percent_Identity=35.3260869565217, Blast_Score=97, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 28676; Mature: 28676

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIVERPRKFALVERAPAATFEEERLHRKQRLAATFRLFARYGFDQGLAGHVTVRDPEF
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
PDRFWINPLSTHFSQIKVSDLQLVDHDGNILIGNKPINQAGFVIHSAIHAAHPQVIAAAH
CCCEECCHHHHHHHHEEEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEC
THSTYGKAFSALGRLLDPLTQDSCAFYGDHVLFDPFTGVVLDAEEGKKIAAALGAKKAAI
CCHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEECCCCCEEEECCCCCHHHHHHCCHHHHH
LQNHGLLTVGPTIEATAWWYIAMDNAARTQLLAEAAGPTKPIPHDIAKHTSGQVGTHKGG
HHCCCEEEECCCCEEEEEEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCC
YFSFQPLWDWITAQEPDLFN
EEECHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNEIVERPRKFALVERAPAATFEEERLHRKQRLAATFRLFARYGFDQGLAGHVTVRDPEF
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
PDRFWINPLSTHFSQIKVSDLQLVDHDGNILIGNKPINQAGFVIHSAIHAAHPQVIAAAH
CCCEECCHHHHHHHHEEEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEC
THSTYGKAFSALGRLLDPLTQDSCAFYGDHVLFDPFTGVVLDAEEGKKIAAALGAKKAAI
CCHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEECCCCCEEEECCCCCHHHHHHCCHHHHH
LQNHGLLTVGPTIEATAWWYIAMDNAARTQLLAEAAGPTKPIPHDIAKHTSGQVGTHKGG
HHCCCEEEECCCCEEEEEEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCC
YFSFQPLWDWITAQEPDLFN
EEECHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]